3i58 - Revision history

OCA at 07:37, 6 September 2023

OCA — Wed, 06 Sep 2023 07:37:38 GMT

←Older revision		Revision as of 07:37, 6 September 2023
Line 3:		Line 3:
	<StructureSection load='3i58' size='340' side='right'caption='[[3i58]], [[Resolution\|resolution]] 2.69Å' scene=''>		<StructureSection load='3i58' size='340' side='right'caption='[[3i58]], [[Resolution\|resolution]] 2.69Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3i58]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"streptomyces_carzinostaticus_subsp~~._neocarzinostaticus~~"_ishida_et_al. "streptomyces~~ carzinostaticus subsp. neocarzinostaticus~~" ishida et al.~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I58 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3I58 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3i58]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_carzinostaticus_subsp._neocarzinostaticus Streptomyces carzinostaticus subsp. neocarzinostaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I58 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3I58 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=7NA:2-HYDROXY-7-METHOXY-5-METHYL+NAPHTHOIC+ACID'>7NA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.69Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3i53\|3i53]], [[3i5u\|3i5u]], [[3i64\|3i64]]</div></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=7NA:2-HYDROXY-7-METHOXY-5-METHYL+NAPHTHOIC+ACID'>7NA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ncsb1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=167636 "Streptomyces carzinostaticus subsp. neocarzinostaticus" Ishida et al.])</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3i58 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i58 OCA], [https://pdbe.org/3i58 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3i58 RCSB], [https://www.ebi.ac.uk/pdbsum/3i58 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3i58 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3i58 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i58 OCA], [https://pdbe.org/3i58 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3i58 RCSB], [https://www.ebi.ac.uk/pdbsum/3i58 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3i58 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/NCSB1_STRCZ NCSB1_STRCZ]] S-adenosyl-L-methionine-dependent O-methyltransferase that catalyzes regiospecific methylation at the 7-hydroxy group of 2,7-dihydroxy-5-methyl-1-naphthoate in the biosynthesis of the naphthoate moiety of the neocarzinostatin chromophore. Also recognizes other dihydroxynaphthoate as substrates and catalyzes their regiospecific O-methylation. The carboxylate and its ortho-hydroxy groups of the substrate appear to be crucial for NcsB1 substrate recognition and binding, and O-methylation takes place only at the free hydroxy group of these dihydroxynaphthoic acids.<ref>PMID:18387946</ref>	+	[https://www.uniprot.org/uniprot/NCSB1_STRCZ NCSB1_STRCZ] S-adenosyl-L-methionine-dependent O-methyltransferase that catalyzes regiospecific methylation at the 7-hydroxy group of 2,7-dihydroxy-5-methyl-1-naphthoate in the biosynthesis of the naphthoate moiety of the neocarzinostatin chromophore. Also recognizes other dihydroxynaphthoate as substrates and catalyzes their regiospecific O-methylation. The carboxylate and its ortho-hydroxy groups of the substrate appear to be crucial for NcsB1 substrate recognition and binding, and O-methylation takes place only at the free hydroxy group of these dihydroxynaphthoic acids.<ref>PMID:18387946</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Streptomyces carzinostaticus subsp. neocarzinostaticus ishida et al]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Bruner, S D~~]]	+	[[Category: Streptomyces carzinostaticus subsp. neocarzinostaticus]]
-	[[Category: ~~Cooke, H A~~]]	+	[[Category: Bruner SD]]
-	[[Category: ~~Methyltransferase]]~~	+	[[Category: Cooke HA]]
-	~~[[Category: Rossmann-like fold]]~~	+
-	~~[[Category: Ternary complex]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 10:57, 13 April 2022

OCA — Wed, 13 Apr 2022 10:57:59 GMT

←Older revision		Revision as of 10:57, 13 April 2022
Line 1:		Line 1:

	==Crystal structure of an O-methyltransferase (NcsB1) from neocarzinostatin biosynthesis in complex with S-adenosyl-L-homocysteine (SAH) and 2-hydroxy-7-methoxy-5-methyl naphthoic acid (NA)==		==Crystal structure of an O-methyltransferase (NcsB1) from neocarzinostatin biosynthesis in complex with S-adenosyl-L-homocysteine (SAH) and 2-hydroxy-7-methoxy-5-methyl naphthoic acid (NA)==
-	<StructureSection load='3i58' size='340' side='right' caption='[[3i58]], [[Resolution\|resolution]] 2.69Å' scene=''>	+	<StructureSection load='3i58' size='340' side='right'caption='[[3i58]], [[Resolution\|resolution]] 2.69Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3i58]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"streptomyces_carzinostaticus_subsp._neocarzinostaticus"_ishida_et_al. "streptomyces carzinostaticus subsp. neocarzinostaticus" ishida et al.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I58 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3I58 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3i58]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"streptomyces_carzinostaticus_subsp._neocarzinostaticus"_ishida_et_al. "streptomyces carzinostaticus subsp. neocarzinostaticus" ishida et al.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I58 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3I58 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=7NA:2-HYDROXY-7-METHOXY-5-METHYL+NAPHTHOIC+ACID'>7NA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=7NA:2-HYDROXY-7-METHOXY-5-METHYL+NAPHTHOIC+ACID'>7NA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3i53\|3i53]], [[3i5u\|3i5u]], [[3i64\|3i64]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3i53\|3i53]], [[3i5u\|3i5u]], [[3i64\|3i64]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ncsb1 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=167636 "Streptomyces carzinostaticus subsp. neocarzinostaticus" Ishida et al.])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ncsb1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=167636 "Streptomyces carzinostaticus subsp. neocarzinostaticus" Ishida et al.])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3i58 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i58 OCA], [~~http~~://pdbe.org/3i58 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3i58 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3i58 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3i58 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3i58 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i58 OCA], [https://pdbe.org/3i58 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3i58 RCSB], [https://www.ebi.ac.uk/pdbsum/3i58 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3i58 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/NCSB1_STRCZ NCSB1_STRCZ]] S-adenosyl-L-methionine-dependent O-methyltransferase that catalyzes regiospecific methylation at the 7-hydroxy group of 2,7-dihydroxy-5-methyl-1-naphthoate in the biosynthesis of the naphthoate moiety of the neocarzinostatin chromophore. Also recognizes other dihydroxynaphthoate as substrates and catalyzes their regiospecific O-methylation. The carboxylate and its ortho-hydroxy groups of the substrate appear to be crucial for NcsB1 substrate recognition and binding, and O-methylation takes place only at the free hydroxy group of these dihydroxynaphthoic acids.<ref>PMID:18387946</ref>	+	[[https://www.uniprot.org/uniprot/NCSB1_STRCZ NCSB1_STRCZ]] S-adenosyl-L-methionine-dependent O-methyltransferase that catalyzes regiospecific methylation at the 7-hydroxy group of 2,7-dihydroxy-5-methyl-1-naphthoate in the biosynthesis of the naphthoate moiety of the neocarzinostatin chromophore. Also recognizes other dihydroxynaphthoate as substrates and catalyzes their regiospecific O-methylation. The carboxylate and its ortho-hydroxy groups of the substrate appear to be crucial for NcsB1 substrate recognition and binding, and O-methylation takes place only at the free hydroxy group of these dihydroxynaphthoic acids.<ref>PMID:18387946</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Streptomyces carzinostaticus subsp. neocarzinostaticus ishida et al]]		[[Category: Streptomyces carzinostaticus subsp. neocarzinostaticus ishida et al]]
		+	[[Category: Large Structures]]
	[[Category: Bruner, S D]]		[[Category: Bruner, S D]]
	[[Category: Cooke, H A]]		[[Category: Cooke, H A]]

OCA at 09:49, 19 December 2018

OCA — Wed, 19 Dec 2018 09:49:06 GMT

←Older revision		Revision as of 09:49, 19 December 2018
Line 1:		Line 1:
		+
	==Crystal structure of an O-methyltransferase (NcsB1) from neocarzinostatin biosynthesis in complex with S-adenosyl-L-homocysteine (SAH) and 2-hydroxy-7-methoxy-5-methyl naphthoic acid (NA)==		==Crystal structure of an O-methyltransferase (NcsB1) from neocarzinostatin biosynthesis in complex with S-adenosyl-L-homocysteine (SAH) and 2-hydroxy-7-methoxy-5-methyl naphthoic acid (NA)==
	<StructureSection load='3i58' size='340' side='right' caption='[[3i58]], [[Resolution\|resolution]] 2.69Å' scene=''>		<StructureSection load='3i58' size='340' side='right' caption='[[3i58]], [[Resolution\|resolution]] 2.69Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3i53\|3i53]], [[3i5u\|3i5u]], [[3i64\|3i64]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3i53\|3i53]], [[3i5u\|3i5u]], [[3i64\|3i64]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ncsb1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=167636 "Streptomyces carzinostaticus subsp. neocarzinostaticus" Ishida et al.])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ncsb1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=167636 "Streptomyces carzinostaticus subsp. neocarzinostaticus" Ishida et al.])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3i58 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i58 OCA], [http://pdbe.org/3i58 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3i58 RCSB], [http://www.ebi.ac.uk/pdbsum/3i58 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3i58 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i58 OCA], [http://pdbe.org/3i58 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3i58 RCSB], [http://www.ebi.ac.uk/pdbsum/3i58 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3i58 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 14:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i5/3i58_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i5/3i58_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 21:03, 7 February 2016

OCA — Sun, 07 Feb 2016 21:03:28 GMT

←Older revision		Revision as of 21:03, 7 February 2016
Line 2:		Line 2:
	<StructureSection load='3i58' size='340' side='right' caption='[[3i58]], [[Resolution\|resolution]] 2.69Å' scene=''>		<StructureSection load='3i58' size='340' side='right' caption='[[3i58]], [[Resolution\|resolution]] 2.69Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3i58]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Streptomyces_carzinostaticus_subsp~~._neocarzinostaticus ~~Streptomyces~~ carzinostaticus subsp. neocarzinostaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I58 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3I58 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3i58]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"streptomyces_carzinostaticus_subsp._neocarzinostaticus"_ishida_et_al. "streptomyces carzinostaticus subsp. neocarzinostaticus" ishida et al.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I58 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3I58 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=7NA:2-HYDROXY-7-METHOXY-5-METHYL+NAPHTHOIC+ACID'>7NA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=7NA:2-HYDROXY-7-METHOXY-5-METHYL+NAPHTHOIC+ACID'>7NA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3i53\|3i53]], [[3i5u\|3i5u]], [[3i64\|3i64]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3i53\|3i53]], [[3i5u\|3i5u]], [[3i64\|3i64]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ncsb1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=167636 Streptomyces carzinostaticus subsp. neocarzinostaticus])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ncsb1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=167636 "Streptomyces carzinostaticus subsp. neocarzinostaticus" Ishida et al.])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3i58 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i58 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3i58 RCSB], [http://www.ebi.ac.uk/pdbsum/3i58 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3i58 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i58 OCA], [http://pdbe.org/3i58 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3i58 RCSB], [http://www.ebi.ac.uk/pdbsum/3i58 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/~~Q84HC8_STRCZ Q84HC8_STRCZ~~]] S-adenosyl-L-methionine-dependent O-methyltransferase that catalyzes regiospecific methylation at the 7-hydroxy group of 2,7-dihydroxy-5-methyl-1-naphthoate in the biosynthesis of the naphthoate moiety of the neocarzinostatin chromophore. Also recognizes other dihydroxynaphthoate as substrates and catalyzes their regiospecific O-methylation. The carboxylate and its ortho-hydroxy groups of the substrate appear to be crucial for NcsB1 substrate recognition and binding, and O-methylation takes place only at the free hydroxy group of these dihydroxynaphthoic acids.<ref>PMID:18387946</ref>	+	[[http://www.uniprot.org/uniprot/NCSB1_STRCZ NCSB1_STRCZ]] S-adenosyl-L-methionine-dependent O-methyltransferase that catalyzes regiospecific methylation at the 7-hydroxy group of 2,7-dihydroxy-5-methyl-1-naphthoate in the biosynthesis of the naphthoate moiety of the neocarzinostatin chromophore. Also recognizes other dihydroxynaphthoate as substrates and catalyzes their regiospecific O-methylation. The carboxylate and its ortho-hydroxy groups of the substrate appear to be crucial for NcsB1 substrate recognition and binding, and O-methylation takes place only at the free hydroxy group of these dihydroxynaphthoic acids.<ref>PMID:18387946</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 18:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3i58 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3i58" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Streptomyces carzinostaticus subsp. neocarzinostaticus]]	+	[[Category: Streptomyces carzinostaticus subsp. neocarzinostaticus ishida et al]]
	[[Category: Bruner, S D]]		[[Category: Bruner, S D]]
	[[Category: Cooke, H A]]		[[Category: Cooke, H A]]

OCA at 18:14, 24 December 2014

OCA — Wed, 24 Dec 2014 18:14:16 GMT

←Older revision		Revision as of 18:14, 24 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3i58 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i58 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3i58 RCSB], [http://www.ebi.ac.uk/pdbsum/3i58 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3i58 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i58 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3i58 RCSB], [http://www.ebi.ac.uk/pdbsum/3i58 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/Q84HC8_STRCZ Q84HC8_STRCZ]] S-adenosyl-L-methionine-dependent O-methyltransferase that catalyzes regiospecific methylation at the 7-hydroxy group of 2,7-dihydroxy-5-methyl-1-naphthoate in the biosynthesis of the naphthoate moiety of the neocarzinostatin chromophore. Also recognizes other dihydroxynaphthoate as substrates and catalyzes their regiospecific O-methylation. The carboxylate and its ortho-hydroxy groups of the substrate appear to be crucial for NcsB1 substrate recognition and binding, and O-methylation takes place only at the free hydroxy group of these dihydroxynaphthoic acids.<ref>PMID:18387946</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 05:59, 18 December 2014

OCA — Thu, 18 Dec 2014 05:59:57 GMT

←Older revision		Revision as of 05:59, 18 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3i58\| PDB=3i58 \| SCENE= }}~~	+	==Crystal structure of an O-methyltransferase (NcsB1) from neocarzinostatin biosynthesis in complex with S-adenosyl-L-homocysteine (SAH) and 2-hydroxy-7-methoxy-5-methyl naphthoic acid (NA)==
-	===Crystal structure of an O-methyltransferase (NcsB1) from neocarzinostatin biosynthesis in complex with S-adenosyl-L-homocysteine (SAH) and 2-hydroxy-7-methoxy-5-methyl naphthoic acid (NA)===	+	<StructureSection load='3i58' size='340' side='right' caption='[[3i58]], [[Resolution\|resolution]] 2.69Å' scene=''>
-	~~{{ABSTRACT_PUBMED_19702337}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3i58]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_carzinostaticus_subsp._neocarzinostaticus Streptomyces carzinostaticus subsp. neocarzinostaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I58 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3I58 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=7NA:2-HYDROXY-7-METHOXY-5-METHYL+NAPHTHOIC+ACID'>7NA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3i53\|3i53]], [[3i5u\|3i5u]], [[3i64\|3i64]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ncsb1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=167636 Streptomyces carzinostaticus subsp. neocarzinostaticus])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3i58 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i58 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3i58 RCSB], [http://www.ebi.ac.uk/pdbsum/3i58 PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i5/3i58_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The small molecule component of the chromoprotein enediyne antitumor antibiotics is biosynthesized through a convergent route, incorporating amino acid, polyketide and carbohydrate building blocks around a central enediyne hydrocarbon core. The naphthoic acid moiety of the enediyne neocarzinostatin plays key roles in the biological activity of the natural product by interacting with both the carrier protein and duplex DNA at the site of action. We have previously described the in vitro characterization of an S-adenosylmethionine-dependent O-methyltransferase (NcsB1) in the neocarzinostatin biosynthetic pathway (Luo, Y.; Lin, S.; Zhang, J.; Cooke, H. A.; Bruner, S. D. and Shen, B. (2008) J. Biol. Chem. 283, 14694-14702). Here we provide a structural basis for NcsB1 activity, illustrating that the enzyme shares an overall architecture with a large family of S-adenosylmethionine-dependent proteins. In addition, NcsB1 represents the first enzyme to be structurally characterized in the biosynthetic pathway of neocarzinostatin. By co-crystallizing the enzyme with various combinations of the cofactor and substrate analogs, details of the active site structure have been established. Changes in subdomain orientation were observed by comparing structures in the presence and absence of substrate, suggesting that reorientation of the enzyme is involved in binding the substrate. In addition, residues important for substrate discrimination were predicted and probed through site directed mutagenesis and in vitro biochemical characterization.

-	~~==About this Structure==~~	+	Molecular basis of substrate promiscuity for the SAM-dependent O-methyltransferase NcsB1, involved in the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin.,Cooke HA, Guenther EL, Luo Y, Shen B, Bruner SD Biochemistry. 2009 Aug 25. PMID:19702337<ref>PMID:19702337</ref>
-	~~[[3i58]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Streptomyces_carzinostaticus_subsp~~.~~_neocarzinostaticus Streptomyces carzinostaticus subsp. neocarzinostaticus]. Full crystallographic information is available from [http~~:/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3I58 OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	<~~ref group="xtra">PMID:019702337<~~/~~ref~~><~~references group="xtra"~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Streptomyces carzinostaticus subsp. neocarzinostaticus]]		[[Category: Streptomyces carzinostaticus subsp. neocarzinostaticus]]
-	[[Category: Bruner, S D.]]	+	[[Category: Bruner, S D]]
-	[[Category: Cooke, H A.]]	+	[[Category: Cooke, H A]]
	[[Category: Methyltransferase]]		[[Category: Methyltransferase]]
	[[Category: Rossmann-like fold]]		[[Category: Rossmann-like fold]]
	[[Category: Ternary complex]]		[[Category: Ternary complex]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA: Protected "3i58" [edit=sysop:move=sysop]

OCA — Wed, 13 Mar 2013 10:41:16 GMT

Protected "3i58" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:41, 13 March 2013

OCA at 10:41, 13 March 2013

OCA — Wed, 13 Mar 2013 10:41:14 GMT

←Older revision		Revision as of 10:41, 13 March 2013
Line 1:		Line 1:
-	{{Seed}}
-	[[Image:3i58.png\|left\|200px]]
-
-	<!--
-	The line below this paragraph, containing "STRUCTURE_3i58", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3i58\| PDB=3i58 \| SCENE= }}		{{STRUCTURE_3i58\| PDB=3i58 \| SCENE= }}
-
	===Crystal structure of an O-methyltransferase (NcsB1) from neocarzinostatin biosynthesis in complex with S-adenosyl-L-homocysteine (SAH) and 2-hydroxy-7-methoxy-5-methyl naphthoic acid (NA)===		===Crystal structure of an O-methyltransferase (NcsB1) from neocarzinostatin biosynthesis in complex with S-adenosyl-L-homocysteine (SAH) and 2-hydroxy-7-methoxy-5-methyl naphthoic acid (NA)===
-
-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_19702337}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 19702337 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_19702337}}		{{ABSTRACT_PUBMED_19702337}}

	==About this Structure==		==About this Structure==
-	~~3I58~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Streptomyces_carzinostaticus_subsp._neocarzinostaticus Streptomyces carzinostaticus subsp. neocarzinostaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I58 OCA].	+	[[3i58]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_carzinostaticus_subsp._neocarzinostaticus Streptomyces carzinostaticus subsp. neocarzinostaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I58 OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~19702337~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019702337</ref><references group="xtra"/>
	[[Category: Streptomyces carzinostaticus subsp. neocarzinostaticus]]		[[Category: Streptomyces carzinostaticus subsp. neocarzinostaticus]]
	[[Category: Bruner, S D.]]		[[Category: Bruner, S D.]]
Line 31:		Line 15:
	[[Category: Ternary complex]]		[[Category: Ternary complex]]
	[[Category: Transferase]]		[[Category: Transferase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct 21 09:39:22 2009''

OCA at 07:39, 21 October 2009

OCA — Wed, 21 Oct 2009 07:39:22 GMT

←Older revision		Revision as of 07:39, 21 October 2009
Line 1:		Line 1:
	{{Seed}}		{{Seed}}
-	[[Image:3i58.~~jpg~~\|left\|200px]]	+	[[Image:3i58.png\|left\|200px]]

	<!--		<!--
Line 32:		Line 32:
	[[Category: Transferase]]		[[Category: Transferase]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Thu Sep 3 15~~:36:09 2009''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct 21 09:39:22 2009''

OCA at 12:36, 3 September 2009

OCA — Thu, 03 Sep 2009 12:36:12 GMT

←Older revision		Revision as of 12:36, 3 September 2009
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3i58.jpg\|left\|200px]]

-	The ~~entry 3i58~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3i58", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3i58\| PDB=3i58 \| SCENE= }}

-	~~Authors: Cooke, H.A., Bruner,~~ S~~.D.~~	+	===Crystal structure of an O-methyltransferase (NcsB1) from neocarzinostatin biosynthesis in complex with S-adenosyl-L-homocysteine (SAH) and 2-hydroxy-7-methoxy-5-methyl naphthoic acid (NA)===

-	Description: Crystal structure of an O-methyltransferase (NcsB1) from neocarzinostatin biosynthesis in complex with S-adenosyl-L-homocysteine (SAH) and 2-hydroxy-7-methoxy-5-methyl naphthoic acid (NA)

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Aug~~ ~~5 09~~:47:39 2009''	+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_19702337}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 19702337 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_19702337}}
		+
		+	==About this Structure==
		+	3I58 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Streptomyces_carzinostaticus_subsp._neocarzinostaticus Streptomyces carzinostaticus subsp. neocarzinostaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I58 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:19702337</ref><references group="xtra"/>
		+	[[Category: Streptomyces carzinostaticus subsp. neocarzinostaticus]]
		+	[[Category: Bruner, S D.]]
		+	[[Category: Cooke, H A.]]
		+	[[Category: Methyltransferase]]
		+	[[Category: Rossmann-like fold]]
		+	[[Category: Ternary complex]]
		+	[[Category: Transferase]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Sep 3 15:36:09 2009''