3i5v - Revision history

OCA at 06:39, 3 April 2024

OCA — Wed, 03 Apr 2024 06:39:07 GMT

←Older revision		Revision as of 06:39, 3 April 2024
Line 3:		Line 3:
	<StructureSection load='3i5v' size='340' side='right'caption='[[3i5v]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='3i5v' size='340' side='right'caption='[[3i5v]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3i5v]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Staphylococcus_aureus_rn4220~~ Staphylococcus aureus ~~rn4220~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I5V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3I5V FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3i5v]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_RN4220 Staphylococcus aureus subsp. aureus RN4220]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I5V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3I5V FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=DGA:DIACYL+GLYCEROL'>DGA</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.8Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'~~>~~[[3i41\|3i41]], [[3i46\|3i46]], [[3i48\|3i48]]~~<~~/div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DGA:DIACYL+GLYCEROL'>DGA</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">hlb ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=561307 Staphylococcus aureus RN4220])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3i5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i5v OCA], [https://pdbe.org/3i5v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3i5v RCSB], [https://www.ebi.ac.uk/pdbsum/3i5v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3i5v ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3i5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i5v OCA], [https://pdbe.org/3i5v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3i5v RCSB], [https://www.ebi.ac.uk/pdbsum/3i5v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3i5v ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/PHLC_STAAU PHLC_STAAU] Bacterial hemolysins are exotoxins that attack blood cell membranes and cause cell rupture. Beta-hemolysin is a phospholipase C with specific activity toward sphingomyelins. Has a high specificity for sphingomyelin, hydrolyzes lysophosphatidylcholine at a much lower rate, but has no activity towards phosphatidylcholine, phosphatidylethanolamine, or phosphatidylserine (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 19:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3i5v ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3i5v ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Beta toxin is a neutral sphingomyelinase secreted by certain strains of Staphylococcus aureus. This virulence factor lyses erythrocytes in order to evade the host immune system as well as scavenge nutrients. The structure of beta toxin was determined at 2.4-A resolution using crystals that were merohedrally twinned. This structure is similar to that of the sphingomyelinases of Listeria ivanovii and Bacillus cereus. Beta toxin belongs to the DNase I folding superfamily; in addition to sphingomyelinases, the proteins most structurally related to beta toxin include human endonuclease HAP1, Escherichia coli endonuclease III, bovine pancreatic DNase I, and the endonuclease domain of TRAS1 from Bombyx mori. Our biological assays demonstrated for the first time that beta toxin kills proliferating human lymphocytes. Structure-directed active site mutations show that biological activities, including hemolysis and lymphotoxicity, are due to the sphingomyelinase activity of the enzyme.
-
-	Structure and biological activities of beta toxin from Staphylococcus aureus.,Huseby M, Shi K, Brown CK, Digre J, Mengistu F, Seo KS, Bohach GA, Schlievert PM, Ohlendorf DH, Earhart CA J Bacteriol. 2007 Dec;189(23):8719-26. Epub 2007 Sep 14. PMID:17873030<ref>PMID:17873030</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3i5v" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Hemolysin 3D structures\|Hemolysin 3D structures]]		*[[Hemolysin 3D structures\|Hemolysin 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Staphylococcus aureus ~~rn4220~~]]	+	[[Category: Staphylococcus aureus subsp. aureus RN4220]]
-	[[Category: Huseby, M]]	+	[[Category: Huseby M]]
-	[[Category: Kruse~~, A C~~]]	+	[[Category: Kruse AC]]
-	[[Category: Ohlendorf~~, D H~~]]	+	[[Category: Ohlendorf DH]]
-	[[Category: Shi, K]]	+	[[Category: Shi K]]
-	~~[[Category: Beta toxin]]~~	+
-	~~[[Category: Hemolysin]]~~	+
-	~~[[Category: Sphingomyelinase]]~~	+
-	~~[[Category: Toxin~~]]	+

OCA at 10:58, 13 April 2022

OCA — Wed, 13 Apr 2022 10:58:34 GMT

←Older revision		Revision as of 10:58, 13 April 2022
Line 1:		Line 1:

	==Crystal structure of beta toxin 275-280 from Staphylococcus aureus==		==Crystal structure of beta toxin 275-280 from Staphylococcus aureus==
-	<StructureSection load='3i5v' size='340' side='right' caption='[[3i5v]], [[Resolution\|resolution]] 2.80Å' scene=''>	+	<StructureSection load='3i5v' size='340' side='right'caption='[[3i5v]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3i5v]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Staphylococcus_aureus_rn4220 Staphylococcus aureus rn4220]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I5V OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3I5V FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3i5v]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_rn4220 Staphylococcus aureus rn4220]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I5V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3I5V FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DGA:DIACYL+GLYCEROL'>DGA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DGA:DIACYL+GLYCEROL'>DGA</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3i41\|3i41]], [[3i46\|3i46]], [[3i48\|3i48]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3i41\|3i41]], [[3i46\|3i46]], [[3i48\|3i48]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">hlb ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=561307 Staphylococcus aureus RN4220])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">hlb ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=561307 Staphylococcus aureus RN4220])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3i5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i5v OCA], [~~http~~://pdbe.org/3i5v PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3i5v RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3i5v PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3i5v ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3i5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i5v OCA], [https://pdbe.org/3i5v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3i5v RCSB], [https://www.ebi.ac.uk/pdbsum/3i5v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3i5v ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 30:		Line 30:

	==See Also==		==See Also==
-	*[[Hemolysin\|Hemolysin]]	+	*[[Hemolysin 3D structures\|Hemolysin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Staphylococcus aureus rn4220]]		[[Category: Staphylococcus aureus rn4220]]
	[[Category: Huseby, M]]		[[Category: Huseby, M]]

OCA at 09:19, 19 December 2018

OCA — Wed, 19 Dec 2018 09:19:45 GMT

←Older revision		Revision as of 09:19, 19 December 2018
Line 13:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i5/3i5v_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i5/3i5v_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 20:20, 9 December 2016

OCA — Fri, 09 Dec 2016 20:20:09 GMT

←Older revision		Revision as of 20:20, 9 December 2016
Line 1:		Line 1:
		+
	==Crystal structure of beta toxin 275-280 from Staphylococcus aureus==		==Crystal structure of beta toxin 275-280 from Staphylococcus aureus==
	<StructureSection load='3i5v' size='340' side='right' caption='[[3i5v]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='3i5v' size='340' side='right' caption='[[3i5v]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3i5v]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Staphylococcus_aureus~~ Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I5V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3I5V FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3i5v]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_rn4220 Staphylococcus aureus rn4220]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I5V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3I5V FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DGA:DIACYL+GLYCEROL'>DGA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DGA:DIACYL+GLYCEROL'>DGA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3i41\|3i41]], [[3i46\|3i46]], [[3i48\|3i48]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3i41\|3i41]], [[3i46\|3i46]], [[3i48\|3i48]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">hlb ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~1280~~ Staphylococcus aureus])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">hlb ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=561307 Staphylococcus aureus RN4220])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3i5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i5v OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3i5v RCSB], [http://www.ebi.ac.uk/pdbsum/3i5v PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3i5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i5v OCA], [http://pdbe.org/3i5v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3i5v RCSB], [http://www.ebi.ac.uk/pdbsum/3i5v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3i5v ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 16:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3i5v ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 26:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3i5v" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 33:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Staphylococcus aureus]]	+	[[Category: Staphylococcus aureus rn4220]]
	[[Category: Huseby, M]]		[[Category: Huseby, M]]
	[[Category: Kruse, A C]]		[[Category: Kruse, A C]]

OCA at 08:43, 8 December 2014

OCA — Mon, 08 Dec 2014 08:43:22 GMT

←Older revision		Revision as of 08:43, 8 December 2014
Line 1:		Line 1:
-	[[~~Image:~~3i5v.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of beta toxin 275-280 from Staphylococcus aureus==
		+	<StructureSection load='3i5v' size='340' side='right' caption='[[3i5v]], [[Resolution\|resolution]] 2.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3i5v]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I5V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3I5V FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DGA:DIACYL+GLYCEROL'>DGA</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3i41\|3i41]], [[3i46\|3i46]], [[3i48\|3i48]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">hlb ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 Staphylococcus aureus])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3i5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i5v OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3i5v RCSB], [http://www.ebi.ac.uk/pdbsum/3i5v PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i5/3i5v_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Beta toxin is a neutral sphingomyelinase secreted by certain strains of Staphylococcus aureus. This virulence factor lyses erythrocytes in order to evade the host immune system as well as scavenge nutrients. The structure of beta toxin was determined at 2.4-A resolution using crystals that were merohedrally twinned. This structure is similar to that of the sphingomyelinases of Listeria ivanovii and Bacillus cereus. Beta toxin belongs to the DNase I folding superfamily; in addition to sphingomyelinases, the proteins most structurally related to beta toxin include human endonuclease HAP1, Escherichia coli endonuclease III, bovine pancreatic DNase I, and the endonuclease domain of TRAS1 from Bombyx mori. Our biological assays demonstrated for the first time that beta toxin kills proliferating human lymphocytes. Structure-directed active site mutations show that biological activities, including hemolysis and lymphotoxicity, are due to the sphingomyelinase activity of the enzyme.

-	~~{{STRUCTURE_3i5v\| PDB=3i5v \| SCENE= }}~~	+	Structure and biological activities of beta toxin from Staphylococcus aureus.,Huseby M, Shi K, Brown CK, Digre J, Mengistu F, Seo KS, Bohach GA, Schlievert PM, Ohlendorf DH, Earhart CA J Bacteriol. 2007 Dec;189(23):8719-26. Epub 2007 Sep 14. PMID:17873030<ref>PMID:17873030</ref>

-	~~===Crystal structure~~ of ~~beta toxin 275-280 from Staphylococcus aureus===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_17873030}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3i5v]] is a 4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Staphylococcus_aureus Staphylococcus aureus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3I5V OCA].~~	+

	==See Also==		==See Also==
	*[[Hemolysin\|Hemolysin]]		*[[Hemolysin\|Hemolysin]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:017873030<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Staphylococcus aureus]]		[[Category: Staphylococcus aureus]]
-	[[Category: Huseby, M.]]	+	[[Category: Huseby, M]]
-	[[Category: Kruse, A C.]]	+	[[Category: Kruse, A C]]
-	[[Category: Ohlendorf, D H.]]	+	[[Category: Ohlendorf, D H]]
-	[[Category: Shi, K.]]	+	[[Category: Shi, K]]
	[[Category: Beta toxin]]		[[Category: Beta toxin]]
	[[Category: Hemolysin]]		[[Category: Hemolysin]]
	[[Category: Sphingomyelinase]]		[[Category: Sphingomyelinase]]
	[[Category: Toxin]]		[[Category: Toxin]]

OCA at 11:29, 20 October 2012

OCA — Sat, 20 Oct 2012 11:29:29 GMT

←Older revision		Revision as of 11:29, 20 October 2012
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	==About this Structure==		==About this Structure==
-	[[3i5v]] is a 4 chain structure ~~of [[Hemolysin]]~~ with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I5V OCA].	+	[[3i5v]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I5V OCA].

	==See Also==		==See Also==

OCA: Protected "3i5v" [edit=sysop:move=sysop]

OCA — Thu, 26 Jul 2012 05:34:08 GMT

Protected "3i5v" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:34, 26 July 2012

OCA at 05:34, 26 July 2012

OCA — Thu, 26 Jul 2012 05:34:06 GMT

←Older revision		Revision as of 05:34, 26 July 2012
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-	~~{{Seed}}~~	+	[[Image:3i5v.png\|left\|200px]]
-	[[Image:3i5v.~~jpg~~\|left\|200px]]	+

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3i5v", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3i5v\| PDB=3i5v \| SCENE= }}		{{STRUCTURE_3i5v\| PDB=3i5v \| SCENE= }}

	===Crystal structure of beta toxin 275-280 from Staphylococcus aureus===		===Crystal structure of beta toxin 275-280 from Staphylococcus aureus===

		+	{{ABSTRACT_PUBMED_17873030}}

	==About this Structure==		==About this Structure==
-	~~3I5V~~ is a 4 ~~chains~~ structure with ~~sequences~~ from [http://en.wikipedia.org/wiki/~~Staphylococcus_aureus_rn4220~~ Staphylococcus aureus ~~rn4220~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I5V OCA].	+	[[3i5v]] is a 4 chain structure of [[Hemolysin]] with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I5V OCA].
		+
		+	==See Also==
		+	*[[Hemolysin\|Hemolysin]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~17873030~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:017873030</ref><references group="xtra"/>
-	[[Category: Staphylococcus aureus ~~rn4220~~]]	+	[[Category: Staphylococcus aureus]]
	[[Category: Huseby, M.]]		[[Category: Huseby, M.]]
	[[Category: Kruse, A C.]]		[[Category: Kruse, A C.]]
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	[[Category: Sphingomyelinase]]		[[Category: Sphingomyelinase]]
	[[Category: Toxin]]		[[Category: Toxin]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug 11 23:53:07 2010''

OCA at 19:45, 11 August 2010

OCA — Wed, 11 Aug 2010 19:45:51 GMT

←Older revision		Revision as of 19:45, 11 August 2010
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-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3i5v.jpg\|left\|200px]]

-	The ~~entry 3i5v~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3i5v", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3i5v\| PDB=3i5v \| SCENE= }}

-	~~Authors: Huseby, M., Shi, K., Kruse, A.C., Ohlendorf, D.H.~~	+	===Crystal structure of beta toxin 275-280 from Staphylococcus aureus===

-	Description: Crystal structure of beta toxin 275-280 from Staphylococcus aureus

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Nov 18 18~~:45:~~03 2009~~''	+	==About this Structure==
		+	3I5V is a 4 chains structure with sequences from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_rn4220 Staphylococcus aureus rn4220]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I5V OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:17873030</ref><references group="xtra"/>
		+	[[Category: Staphylococcus aureus rn4220]]
		+	[[Category: Huseby, M.]]
		+	[[Category: Kruse, A C.]]
		+	[[Category: Ohlendorf, D H.]]
		+	[[Category: Shi, K.]]
		+	[[Category: Beta toxin]]
		+	[[Category: Hemolysin]]
		+	[[Category: Sphingomyelinase]]
		+	[[Category: Toxin]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug 11 23:53:07 2010''

OCA at 16:45, 18 November 2009

OCA — Wed, 18 Nov 2009 16:45:04 GMT

←Older revision		Revision as of 16:45, 18 November 2009
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3i5v is ON HOLD until ~~sometime in the future~~	+	The entry 3i5v is ON HOLD until Paper Publication

-	Authors: Huseby, M., Shi, K., Kruse, A.C., Ohlendorf, D. H.	+	Authors: Huseby, M., Shi, K., Kruse, A.C., Ohlendorf, D.H.

-	Description: ~~Beta~~ toxin 275-280 from Staphylococcus aureus	+	Description: Crystal structure of beta toxin 275-280 from Staphylococcus aureus

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Jul 15 08~~:30:12 2009''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Nov 18 18:45:03 2009''