3iey - Revision history

OCA at 07:45, 6 September 2023

OCA — Wed, 06 Sep 2023 07:45:46 GMT

←Older revision		Revision as of 07:45, 6 September 2023
Line 3:		Line 3:
	<StructureSection load='3iey' size='340' side='right'caption='[[3iey]], [[Resolution\|resolution]] 2.11Å' scene=''>		<StructureSection load='3iey' size='340' side='right'caption='[[3iey]], [[Resolution\|resolution]] 2.11Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3iey]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"nanoarchaeum_equitans"_huber_et_al._2002 "nanoarchaeum~~ equitans~~" huber et al. 2002~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IEY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IEY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3iey]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Nanoarchaeum_equitans Nanoarchaeum equitans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IEY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IEY FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[3if0\|3if0]]</div></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.11Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">endA, NEQ205 ([https://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&srchmode=5&id=160232 "Nanoarchaeum equitans" Huber et al. 2002]), NEQ261 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=160232 "Nanoarchaeum equitans" Huber et al. 2002])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/tRNA-intron_endonuclease tRNA-intron endonuclease], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.9 3.1.27.9] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3iey FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3iey OCA], [https://pdbe.org/3iey PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3iey RCSB], [https://www.ebi.ac.uk/pdbsum/3iey PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3iey ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3iey FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3iey OCA], [https://pdbe.org/3iey PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3iey RCSB], [https://www.ebi.ac.uk/pdbsum/3iey PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3iey ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/ENDA_NANEQ ENDA_NANEQ]] Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp (By similarity).	+	[https://www.uniprot.org/uniprot/ENDA_NANEQ ENDA_NANEQ] Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 28:
	</div>		</div>
	<div class="pdbe-citations 3iey" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3iey" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Endonuclease 3D structures\|Endonuclease 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Nanoarchaeum equitans huber et al. 2002]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~TRNA-intron endonuclease~~]]	+	[[Category: Nanoarchaeum equitans]]
-	[[Category: Li, H]]	+	[[Category: Li H]]
-	[[Category: Mitchell, M]]	+	[[Category: Mitchell M]]
-	~~[[Category: Endonuclease]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Hydrolase-rna binding protein complex]]~~	+
-	~~[[Category: Nuclease]]~~	+
-	~~[[Category: Protein heterodimer]]~~	+
-	~~[[Category: Trna processing~~]]	+

OCA at 12:10, 13 October 2021

OCA — Wed, 13 Oct 2021 12:10:10 GMT

←Older revision		Revision as of 12:10, 13 October 2021
Line 1:		Line 1:

	==Crystal Structure of the functional Nanoarchaeum equitans tRNA splicing endonuclease==		==Crystal Structure of the functional Nanoarchaeum equitans tRNA splicing endonuclease==
-	<StructureSection load='3iey' size='340' side='right' caption='[[3iey]], [[Resolution\|resolution]] 2.11Å' scene=''>	+	<StructureSection load='3iey' size='340' side='right'caption='[[3iey]], [[Resolution\|resolution]] 2.11Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3iey]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"nanoarchaeum_equitans"_huber_et_al._2002 "nanoarchaeum equitans" huber et al. 2002]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IEY OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3IEY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3iey]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"nanoarchaeum_equitans"_huber_et_al._2002 "nanoarchaeum equitans" huber et al. 2002]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IEY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IEY FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3if0\|3if0]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3if0\|3if0]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">endA, NEQ205 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=160232 "Nanoarchaeum equitans" Huber et al. 2002]), NEQ261 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=160232 "Nanoarchaeum equitans" Huber et al. 2002])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">endA, NEQ205 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=160232 "Nanoarchaeum equitans" Huber et al. 2002]), NEQ261 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=160232 "Nanoarchaeum equitans" Huber et al. 2002])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/tRNA-intron_endonuclease tRNA-intron endonuclease], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.9 3.1.27.9] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/tRNA-intron_endonuclease tRNA-intron endonuclease], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.9 3.1.27.9] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3iey FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3iey OCA], [~~http~~://pdbe.org/3iey PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3iey RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3iey PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3iey ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3iey FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3iey OCA], [https://pdbe.org/3iey PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3iey RCSB], [https://www.ebi.ac.uk/pdbsum/3iey PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3iey ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/ENDA_NANEQ ENDA_NANEQ]] Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp (By similarity).	+	[[https://www.uniprot.org/uniprot/ENDA_NANEQ ENDA_NANEQ]] Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 32:

	==See Also==		==See Also==
-	*[[Endonuclease\|Endonuclease]]	+	*[[Endonuclease 3D structures\|Endonuclease 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Nanoarchaeum equitans huber et al. 2002]]		[[Category: Nanoarchaeum equitans huber et al. 2002]]
		+	[[Category: Large Structures]]
	[[Category: TRNA-intron endonuclease]]		[[Category: TRNA-intron endonuclease]]
	[[Category: Li, H]]		[[Category: Li, H]]

OCA at 10:07, 19 December 2018

OCA — Wed, 19 Dec 2018 10:07:13 GMT

←Older revision		Revision as of 10:07, 19 December 2018
Line 1:		Line 1:
		+
	==Crystal Structure of the functional Nanoarchaeum equitans tRNA splicing endonuclease==		==Crystal Structure of the functional Nanoarchaeum equitans tRNA splicing endonuclease==
	<StructureSection load='3iey' size='340' side='right' caption='[[3iey]], [[Resolution\|resolution]] 2.11Å' scene=''>		<StructureSection load='3iey' size='340' side='right' caption='[[3iey]], [[Resolution\|resolution]] 2.11Å' scene=''>
Line 6:		Line 7:
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">endA, NEQ205 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=160232 "Nanoarchaeum equitans" Huber et al. 2002]), NEQ261 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=160232 "Nanoarchaeum equitans" Huber et al. 2002])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">endA, NEQ205 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=160232 "Nanoarchaeum equitans" Huber et al. 2002]), NEQ261 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=160232 "Nanoarchaeum equitans" Huber et al. 2002])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/tRNA-intron_endonuclease tRNA-intron endonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.9 3.1.27.9] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/tRNA-intron_endonuclease tRNA-intron endonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.9 3.1.27.9] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3iey FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3iey OCA], [http://pdbe.org/3iey PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3iey RCSB], [http://www.ebi.ac.uk/pdbsum/3iey PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3iey FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3iey OCA], [http://pdbe.org/3iey PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3iey RCSB], [http://www.ebi.ac.uk/pdbsum/3iey PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3iey ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ie/3iey_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ie/3iey_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 13:50, 7 February 2016

OCA — Sun, 07 Feb 2016 13:50:58 GMT

←Older revision		Revision as of 13:50, 7 February 2016
Line 2:		Line 2:
	<StructureSection load='3iey' size='340' side='right' caption='[[3iey]], [[Resolution\|resolution]] 2.11Å' scene=''>		<StructureSection load='3iey' size='340' side='right' caption='[[3iey]], [[Resolution\|resolution]] 2.11Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3iey]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Nanoarchaeum_equitans Nanoarchaeum~~ equitans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IEY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3IEY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3iey]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"nanoarchaeum_equitans"_huber_et_al._2002 "nanoarchaeum equitans" huber et al. 2002]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IEY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3IEY FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3if0\|3if0]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3if0\|3if0]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">endA, NEQ205 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=160232 Nanoarchaeum equitans]), NEQ261 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=160232 Nanoarchaeum equitans])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">endA, NEQ205 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=160232 "Nanoarchaeum equitans" Huber et al. 2002]), NEQ261 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=160232 "Nanoarchaeum equitans" Huber et al. 2002])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/tRNA-intron_endonuclease tRNA-intron endonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.9 3.1.27.9] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/tRNA-intron_endonuclease tRNA-intron endonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.9 3.1.27.9] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3iey FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3iey OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3iey RCSB], [http://www.ebi.ac.uk/pdbsum/3iey PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3iey FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3iey OCA], [http://pdbe.org/3iey PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3iey RCSB], [http://www.ebi.ac.uk/pdbsum/3iey PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3iey ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3iey" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 35:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Nanoarchaeum equitans]]	+	[[Category: Nanoarchaeum equitans huber et al. 2002]]
	[[Category: TRNA-intron endonuclease]]		[[Category: TRNA-intron endonuclease]]
	[[Category: Li, H]]		[[Category: Li, H]]

OCA at 15:38, 24 December 2014

OCA — Wed, 24 Dec 2014 15:38:03 GMT

←Older revision		Revision as of 15:38, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3iey]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Nanoarchaeum_equitans Nanoarchaeum equitans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IEY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3IEY FirstGlance]. <br>		<table><tr><td colspan='2'>[[3iey]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Nanoarchaeum_equitans Nanoarchaeum equitans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IEY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3IEY FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3if0\|3if0]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3if0\|3if0]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">endA, NEQ205 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=160232 Nanoarchaeum equitans]), NEQ261 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=160232 Nanoarchaeum equitans])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">endA, NEQ205 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=160232 Nanoarchaeum equitans]), NEQ261 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=160232 Nanoarchaeum equitans])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/tRNA-intron_endonuclease tRNA-intron endonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.9 3.1.27.9] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/tRNA-intron_endonuclease tRNA-intron endonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.9 3.1.27.9] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3iey FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3iey OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3iey RCSB], [http://www.ebi.ac.uk/pdbsum/3iey PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3iey FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3iey OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3iey RCSB], [http://www.ebi.ac.uk/pdbsum/3iey PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ENDA_NANEQ ENDA_NANEQ]] Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 37:
	[[Category: Nanoarchaeum equitans]]		[[Category: Nanoarchaeum equitans]]
	[[Category: TRNA-intron endonuclease]]		[[Category: TRNA-intron endonuclease]]
-	[[Category: Li, H.]]	+	[[Category: Li, H]]
-	[[Category: Mitchell, M.]]	+	[[Category: Mitchell, M]]
	[[Category: Endonuclease]]		[[Category: Endonuclease]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 13:31, 29 September 2014

OCA — Mon, 29 Sep 2014 13:31:55 GMT

←Older revision		Revision as of 13:31, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~3iey.~~png~~\|~~left~~\|200px]]	+	==Crystal Structure of the functional Nanoarchaeum equitans tRNA splicing endonuclease==
		+	<StructureSection load='3iey' size='340' side='right' caption='[[3iey]], [[Resolution\|resolution]] 2.11Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3iey]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Nanoarchaeum_equitans Nanoarchaeum equitans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IEY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3IEY FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3if0\|3if0]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">endA, NEQ205 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=160232 Nanoarchaeum equitans]), NEQ261 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=160232 Nanoarchaeum equitans])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/tRNA-intron_endonuclease tRNA-intron endonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.9 3.1.27.9] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3iey FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3iey OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3iey RCSB], [http://www.ebi.ac.uk/pdbsum/3iey PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ie/3iey_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The RNA splicing and processing endonuclease from Nanoarchaeum equitans (NEQ) belongs to the recently identified (alphabeta)(2) family of splicing endonucleases that require two different subunits for splicing activity. N. equitans splicing endonuclease comprises the catalytic subunit (NEQ205) and the structural subunit (NEQ261). Here, we report the crystal structure of the functional NEQ enzyme at 2.1 A containing both subunits, as well as that of the NEQ261 subunit alone at 2.2 A. The functional enzyme resembles previously known alpha(2) and alpha(4) endonucleases but forms a heterotetramer: a dimer of two heterodimers of the catalytic subunit (NEQ205) and the structural subunit (NEQ261). Surprisingly, NEQ261 alone forms a homodimer, similar to the previously known homodimer of the catalytic subunit. The homodimers of isolated subunits are inhibitory to heterodimerization as illustrated by a covalently linked catalytic homodimer that had no RNA cleavage activity upon mixing with the structural subunit. Detailed structural comparison reveals a more favorable hetero- than homodimerization interface, thereby suggesting a possible regulation mechanism of enzyme assembly through available subunits. Finally, the uniquely flexible active site of the NEQ endonuclease provides a possible explanation for its broader substrate specificity.

-	~~{{STRUCTURE_3iey\| PDB=3iey \| SCENE= }}~~	+	Crystal structure and assembly of the functional Nanoarchaeum equitans tRNA splicing endonuclease.,Mitchell M, Xue S, Erdman R, Randau L, Soll D, Li H Nucleic Acids Res. 2009 Sep;37(17):5793-802. Epub 2009 Jul 3. PMID:19578064<ref>PMID:19578064</ref>

-	~~===Crystal Structure~~ of the ~~functional Nanoarchaeum equitans tRNA splicing endonuclease===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_19578064}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3iey]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Nanoarchaeum_equitans Nanoarchaeum equitans]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3IEY OCA].~~	+

	==See Also==		==See Also==
	*[[Endonuclease\|Endonuclease]]		*[[Endonuclease\|Endonuclease]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:019578064<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Nanoarchaeum equitans]]		[[Category: Nanoarchaeum equitans]]
	[[Category: TRNA-intron endonuclease]]		[[Category: TRNA-intron endonuclease]]

OCA at 06:49, 20 October 2012

OCA — Sat, 20 Oct 2012 06:49:55 GMT

←Older revision		Revision as of 06:49, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3iey]] is a 2 chain structure ~~of [[Endonuclease]]~~ with sequence from [http://en.wikipedia.org/wiki/Nanoarchaeum_equitans Nanoarchaeum equitans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IEY OCA].	+	[[3iey]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Nanoarchaeum_equitans Nanoarchaeum equitans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IEY OCA].

	==See Also==		==See Also==

OCA: Protected "3iey" [edit=sysop:move=sysop]

OCA — Wed, 25 Jul 2012 14:26:13 GMT

Protected "3iey" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:26, 25 July 2012

OCA at 14:26, 25 July 2012

OCA — Wed, 25 Jul 2012 14:26:11 GMT

←Older revision		Revision as of 14:26, 25 July 2012
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-	{{Seed}}
	[[Image:3iey.png\|left\|200px]]		[[Image:3iey.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3iey", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
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	{{STRUCTURE_3iey\| PDB=3iey \| SCENE= }}		{{STRUCTURE_3iey\| PDB=3iey \| SCENE= }}

	===Crystal Structure of the functional Nanoarchaeum equitans tRNA splicing endonuclease===		===Crystal Structure of the functional Nanoarchaeum equitans tRNA splicing endonuclease===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_19578064}}, adds the Publication Abstract to the page
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	{{ABSTRACT_PUBMED_19578064}}		{{ABSTRACT_PUBMED_19578064}}

	==About this Structure==		==About this Structure==
-	~~3IEY~~ is a 2 ~~chains~~ structure of ~~sequences~~ from [http://en.wikipedia.org/wiki/Nanoarchaeum_equitans Nanoarchaeum equitans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IEY OCA].	+	[[3iey]] is a 2 chain structure of [[Endonuclease]] with sequence from [http://en.wikipedia.org/wiki/Nanoarchaeum_equitans Nanoarchaeum equitans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IEY OCA].
		+
		+	==See Also==
		+	*[[Endonuclease\|Endonuclease]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~19578064~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019578064</ref><references group="xtra"/>
	[[Category: Nanoarchaeum equitans]]		[[Category: Nanoarchaeum equitans]]
	[[Category: TRNA-intron endonuclease]]		[[Category: TRNA-intron endonuclease]]
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	[[Category: Endonuclease]]		[[Category: Endonuclease]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
-	[[Category: Hydrolase/rna binding protein complex]]	+	[[Category: Hydrolase-rna binding protein complex]]
	[[Category: Nuclease]]		[[Category: Nuclease]]
	[[Category: Protein heterodimer]]		[[Category: Protein heterodimer]]
	[[Category: Trna processing]]		[[Category: Trna processing]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Nov 11 20:28:11 2009''

OCA at 18:28, 11 November 2009

OCA — Wed, 11 Nov 2009 18:28:12 GMT

←Older revision		Revision as of 18:28, 11 November 2009
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	{{Seed}}		{{Seed}}
-	[[Image:3iey.~~jpg~~\|left\|200px]]	+	[[Image:3iey.png\|left\|200px]]

	<!--		<!--
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	[[Category: Trna processing]]		[[Category: Trna processing]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Thu Sep 3 15~~:38:08 2009''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Nov 11 20:28:11 2009''