3iiw - Revision history

OCA at 06:39, 3 April 2024

OCA — Wed, 03 Apr 2024 06:39:18 GMT

←Older revision		Revision as of 06:39, 3 April 2024
Line 3:		Line 3:
	<StructureSection load='3iiw' size='340' side='right'caption='[[3iiw]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='3iiw' size='340' side='right'caption='[[3iiw]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3iiw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IIW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IIW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3iiw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IIW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IIW FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"~~><scene name='pdbligand=M3L:N-TRIMETHYLLYSINE'>M3L</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
-	~~<tr~~ id~~='related'><td class~~="~~sblockLbl~~">~~<b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max~~-~~height: 3em;'>[[2qxv\|2qxv]]~~, [[~~3iiy~~\|~~3iiy~~]]~~, [[3ij0\|3ij0]], [[3ij1\|3ij1]], [[3ijc\|3ijc]]</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=M3L:N-TRIMETHYLLYSINE'>M3L</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~EED ([https~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3iiw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3iiw OCA], [https://pdbe.org/3iiw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3iiw RCSB], [https://www.ebi.ac.uk/pdbsum/3iiw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3iiw ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3iiw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3iiw OCA], [https://pdbe.org/3iiw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3iiw RCSB], [https://www.ebi.ac.uk/pdbsum/3iiw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3iiw ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/EED_HUMAN EED_HUMAN]] Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Also recognizes 'Lys-26' trimethylated histone H1 with the effect of inhibiting PRC2 complex methyltransferase activity on nucleosomal histone H3 'Lys-27', whereas H3 'Lys-27' recognition has the opposite effect, enabling the propagation of this repressive mark. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.<ref>PMID:9584199</ref> <ref>PMID:10581039</ref> <ref>PMID:14532106</ref> <ref>PMID:15385962</ref> <ref>PMID:15231737</ref> <ref>PMID:15225548</ref> <ref>PMID:16357870</ref> <ref>PMID:18285464</ref> <ref>PMID:20974918</ref>	+	[https://www.uniprot.org/uniprot/EED_HUMAN EED_HUMAN] Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Also recognizes 'Lys-26' trimethylated histone H1 with the effect of inhibiting PRC2 complex methyltransferase activity on nucleosomal histone H3 'Lys-27', whereas H3 'Lys-27' recognition has the opposite effect, enabling the propagation of this repressive mark. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.<ref>PMID:9584199</ref> <ref>PMID:10581039</ref> <ref>PMID:14532106</ref> <ref>PMID:15385962</ref> <ref>PMID:15231737</ref> <ref>PMID:15225548</ref> <ref>PMID:16357870</ref> <ref>PMID:18285464</ref> <ref>PMID:20974918</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3iiw ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3iiw ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Polycomb group proteins have an essential role in the epigenetic maintenance of repressive chromatin states. The gene-silencing activity of the Polycomb repressive complex 2 (PRC2) depends on its ability to trimethylate lysine 27 of histone H3 (H3K27) by the catalytic SET domain of the EZH2 subunit, and at least two other subunits of the complex: SUZ12 and EED. Here we show that the carboxy-terminal domain of EED specifically binds to histone tails carrying trimethyl-lysine residues associated with repressive chromatin marks, and that this leads to the allosteric activation of the methyltransferase activity of PRC2. Mutations in EED that prevent it from recognizing repressive trimethyl-lysine marks abolish the activation of PRC2 in vitro and, in Drosophila, reduce global methylation and disrupt development. These findings suggest a model for the propagation of the H3K27me3 mark that accounts for the maintenance of repressive chromatin domains and for the transmission of a histone modification from mother to daughter cells.
-
-	Role of the polycomb protein EED in the propagation of repressive histone marks.,Margueron R, Justin N, Ohno K, Sharpe ML, Son J, Drury WJ 3rd, Voigt P, Martin SR, Taylor WR, De Marco V, Pirrotta V, Reinberg D, Gamblin SJ Nature. 2009 Oct 8;461(7265):762-7. Epub 2009 Sep 20. PMID:19767730<ref>PMID:19767730</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3iiw" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
-	*[[Polycomb\|Polycomb]]	+	*[[Polycomb complex proteins 3D structures\|Polycomb complex proteins 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Gamblin, S J~~]]	+	[[Category: De Marco V]]
-	[[Category: ~~Justin, N~~]]	+	[[Category: Gamblin SJ]]
-	[[Category: ~~Marco, V De~~]]	+	[[Category: Justin N]]
-	[[Category: Martin, S]]	+	[[Category: Martin S]]
-	[[Category: Sharpe~~, M L~~]]	+	[[Category: Sharpe ML]]
-	[[Category: Taylor~~, W R]]~~	+	[[Category: Taylor WR]]
-	~~[[Category: Alternative initiation]]~~	+
-	~~[[Category: Alternative splicing]]~~	+
-	~~[[Category: Chromatin regulator]]~~	+
-	~~[[Category: Gene regulation]]~~	+
-	~~[[Category: Nucleus]]~~	+
-	~~[[Category: Phosphoprotein]]~~	+
-	~~[[Category: Repressor]]~~	+
-	~~[[Category: Transcription]]~~	+
-	~~[[Category: Transcription regulation]]~~	+
-	~~[[Category: Wd repeat]]~~	+
-	~~[[Category: Wd40 domain~~]]	+

OCA at 03:33, 21 April 2022

OCA — Thu, 21 Apr 2022 03:33:56 GMT

←Older revision		Revision as of 03:33, 21 April 2022
Line 1:		Line 1:

	==Crystal structure of Eed in complex with a trimethylated histone H3K27 peptide==		==Crystal structure of Eed in complex with a trimethylated histone H3K27 peptide==
-	<StructureSection load='3iiw' size='340' side='right' caption='[[3iiw]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='3iiw' size='340' side='right'caption='[[3iiw]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3iiw]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IIW OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3IIW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3iiw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IIW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IIW FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=M3L:N-TRIMETHYLLYSINE'>M3L</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=M3L:N-TRIMETHYLLYSINE'>M3L</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2qxv\|2qxv]], [[3iiy\|3iiy]], [[3ij0\|3ij0]], [[3ij1\|3ij1]], [[3ijc\|3ijc]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2qxv\|2qxv]], [[3iiy\|3iiy]], [[3ij0\|3ij0]], [[3ij1\|3ij1]], [[3ijc\|3ijc]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EED ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EED ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3iiw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3iiw OCA], [~~http~~://pdbe.org/3iiw PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3iiw RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3iiw PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3iiw ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3iiw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3iiw OCA], [https://pdbe.org/3iiw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3iiw RCSB], [https://www.ebi.ac.uk/pdbsum/3iiw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3iiw ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/EED_HUMAN EED_HUMAN]] Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Also recognizes 'Lys-26' trimethylated histone H1 with the effect of inhibiting PRC2 complex methyltransferase activity on nucleosomal histone H3 'Lys-27', whereas H3 'Lys-27' recognition has the opposite effect, enabling the propagation of this repressive mark. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.<ref>PMID:9584199</ref> <ref>PMID:10581039</ref> <ref>PMID:14532106</ref> <ref>PMID:15385962</ref> <ref>PMID:15231737</ref> <ref>PMID:15225548</ref> <ref>PMID:16357870</ref> <ref>PMID:18285464</ref> <ref>PMID:20974918</ref>	+	[[https://www.uniprot.org/uniprot/EED_HUMAN EED_HUMAN]] Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Also recognizes 'Lys-26' trimethylated histone H1 with the effect of inhibiting PRC2 complex methyltransferase activity on nucleosomal histone H3 'Lys-27', whereas H3 'Lys-27' recognition has the opposite effect, enabling the propagation of this repressive mark. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.<ref>PMID:9584199</ref> <ref>PMID:10581039</ref> <ref>PMID:14532106</ref> <ref>PMID:15385962</ref> <ref>PMID:15231737</ref> <ref>PMID:15225548</ref> <ref>PMID:16357870</ref> <ref>PMID:18285464</ref> <ref>PMID:20974918</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Gamblin, S J]]		[[Category: Gamblin, S J]]
	[[Category: Justin, N]]		[[Category: Justin, N]]

OCA at 09:54, 19 December 2018

OCA — Wed, 19 Dec 2018 09:54:10 GMT

←Older revision		Revision as of 09:54, 19 December 2018
Line 1:		Line 1:
		+
	==Crystal structure of Eed in complex with a trimethylated histone H3K27 peptide==		==Crystal structure of Eed in complex with a trimethylated histone H3K27 peptide==
	<StructureSection load='3iiw' size='340' side='right' caption='[[3iiw]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='3iiw' size='340' side='right' caption='[[3iiw]], [[Resolution\|resolution]] 1.80Å' scene=''>
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	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2qxv\|2qxv]], [[3iiy\|3iiy]], [[3ij0\|3ij0]], [[3ij1\|3ij1]], [[3ijc\|3ijc]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2qxv\|2qxv]], [[3iiy\|3iiy]], [[3ij0\|3ij0]], [[3ij1\|3ij1]], [[3ijc\|3ijc]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EED ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EED ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3iiw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3iiw OCA], [http://pdbe.org/3iiw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3iiw RCSB], [http://www.ebi.ac.uk/pdbsum/3iiw PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3iiw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3iiw OCA], [http://pdbe.org/3iiw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3iiw RCSB], [http://www.ebi.ac.uk/pdbsum/3iiw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3iiw ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 14:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ii/3iiw_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ii/3iiw_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 29:		Line 30:
	</div>		</div>
	<div class="pdbe-citations 3iiw" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3iiw" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Polycomb\|Polycomb]]
	== References ==		== References ==
	<references/>		<references/>
Line 41:		Line 45:
	[[Category: Taylor, W R]]		[[Category: Taylor, W R]]
	[[Category: Alternative initiation]]		[[Category: Alternative initiation]]
		+	[[Category: Alternative splicing]]
	[[Category: Chromatin regulator]]		[[Category: Chromatin regulator]]
	[[Category: Gene regulation]]		[[Category: Gene regulation]]

OCA at 15:27, 9 February 2016

OCA — Tue, 09 Feb 2016 15:27:55 GMT

←Older revision		Revision as of 15:27, 9 February 2016
Line 2:		Line 2:
	<StructureSection load='3iiw' size='340' side='right' caption='[[3iiw]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='3iiw' size='340' side='right' caption='[[3iiw]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3iiw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IIW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3IIW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3iiw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IIW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3IIW FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=M3L:N-TRIMETHYLLYSINE'>M3L</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=M3L:N-TRIMETHYLLYSINE'>M3L</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2qxv\|2qxv]], [[3iiy\|3iiy]], [[3ij0\|3ij0]], [[3ij1\|3ij1]], [[3ijc\|3ijc]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2qxv\|2qxv]], [[3iiy\|3iiy]], [[3ij0\|3ij0]], [[3ij1\|3ij1]], [[3ijc\|3ijc]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EED ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EED ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3iiw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3iiw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3iiw RCSB], [http://www.ebi.ac.uk/pdbsum/3iiw PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3iiw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3iiw OCA], [http://pdbe.org/3iiw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3iiw RCSB], [http://www.ebi.ac.uk/pdbsum/3iiw PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3iiw ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3iiw" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Gamblin, S J]]		[[Category: Gamblin, S J]]
	[[Category: Justin, N]]		[[Category: Justin, N]]

OCA at 07:43, 25 December 2014

OCA — Thu, 25 Dec 2014 07:43:35 GMT

←Older revision		Revision as of 07:43, 25 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3iiw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3iiw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3iiw RCSB], [http://www.ebi.ac.uk/pdbsum/3iiw PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3iiw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3iiw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3iiw RCSB], [http://www.ebi.ac.uk/pdbsum/3iiw PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/EED_HUMAN EED_HUMAN]] Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Also recognizes 'Lys-26' trimethylated histone H1 with the effect of inhibiting PRC2 complex methyltransferase activity on nucleosomal histone H3 'Lys-27', whereas H3 'Lys-27' recognition has the opposite effect, enabling the propagation of this repressive mark. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.<ref>PMID:9584199</ref> <ref>PMID:10581039</ref> <ref>PMID:14532106</ref> <ref>PMID:15385962</ref> <ref>PMID:15231737</ref> <ref>PMID:15225548</ref> <ref>PMID:16357870</ref> <ref>PMID:18285464</ref> <ref>PMID:20974918</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 06:15, 18 December 2014

OCA — Thu, 18 Dec 2014 06:15:12 GMT

←Older revision		Revision as of 06:15, 18 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3iiw\| PDB=3iiw \| SCENE= }}~~	+	==Crystal structure of Eed in complex with a trimethylated histone H3K27 peptide==
-	===Crystal structure of Eed in complex with a trimethylated histone H3K27 peptide===	+	<StructureSection load='3iiw' size='340' side='right' caption='[[3iiw]], [[Resolution\|resolution]] 1.80Å' scene=''>
-	~~{{ABSTRACT_PUBMED_19767730}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3iiw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IIW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3IIW FirstGlance]. <br>
		+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=M3L:N-TRIMETHYLLYSINE'>M3L</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2qxv\|2qxv]], [[3iiy\|3iiy]], [[3ij0\|3ij0]], [[3ij1\|3ij1]], [[3ijc\|3ijc]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EED ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3iiw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3iiw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3iiw RCSB], [http://www.ebi.ac.uk/pdbsum/3iiw PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ii/3iiw_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Polycomb group proteins have an essential role in the epigenetic maintenance of repressive chromatin states. The gene-silencing activity of the Polycomb repressive complex 2 (PRC2) depends on its ability to trimethylate lysine 27 of histone H3 (H3K27) by the catalytic SET domain of the EZH2 subunit, and at least two other subunits of the complex: SUZ12 and EED. Here we show that the carboxy-terminal domain of EED specifically binds to histone tails carrying trimethyl-lysine residues associated with repressive chromatin marks, and that this leads to the allosteric activation of the methyltransferase activity of PRC2. Mutations in EED that prevent it from recognizing repressive trimethyl-lysine marks abolish the activation of PRC2 in vitro and, in Drosophila, reduce global methylation and disrupt development. These findings suggest a model for the propagation of the H3K27me3 mark that accounts for the maintenance of repressive chromatin domains and for the transmission of a histone modification from mother to daughter cells.

-	~~==About this Structure==~~	+	Role of the polycomb protein EED in the propagation of repressive histone marks.,Margueron R, Justin N, Ohno K, Sharpe ML, Son J, Drury WJ 3rd, Voigt P, Martin SR, Taylor WR, De Marco V, Pirrotta V, Reinberg D, Gamblin SJ Nature. 2009 Oct 8;461(7265):762-7. Epub 2009 Sep 20. PMID:19767730<ref>PMID:19767730</ref>
-	~~[[3iiw]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http~~:~~//oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=3IIW OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	<~~ref group="xtra">PMID:019767730<~~/~~ref~~><~~references group="xtra"~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Gamblin, S J.]]	+	[[Category: Gamblin, S J]]
-	[[Category: Justin, N.]]	+	[[Category: Justin, N]]
-	[[Category: Marco, V De.]]	+	[[Category: Marco, V De]]
-	[[Category: Martin, S.]]	+	[[Category: Martin, S]]
-	[[Category: Sharpe, M L.]]	+	[[Category: Sharpe, M L]]
-	[[Category: Taylor, W R.]]	+	[[Category: Taylor, W R]]
	[[Category: Alternative initiation]]		[[Category: Alternative initiation]]
	[[Category: Chromatin regulator]]		[[Category: Chromatin regulator]]

OCA: Protected "3iiw" [edit=sysop:move=sysop]

OCA — Wed, 13 Mar 2013 10:50:40 GMT

Protected "3iiw" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:50, 13 March 2013

OCA at 10:50, 13 March 2013

OCA — Wed, 13 Mar 2013 10:50:37 GMT

←Older revision		Revision as of 10:50, 13 March 2013
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-	{{Seed}}
-	[[Image:3iiw.png\|left\|200px]]
-
-	<!--
-	The line below this paragraph, containing "STRUCTURE_3iiw", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3iiw\| PDB=3iiw \| SCENE= }}		{{STRUCTURE_3iiw\| PDB=3iiw \| SCENE= }}
-
	===Crystal structure of Eed in complex with a trimethylated histone H3K27 peptide===		===Crystal structure of Eed in complex with a trimethylated histone H3K27 peptide===
-
-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_19767730}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 19767730 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_19767730}}		{{ABSTRACT_PUBMED_19767730}}

	==About this Structure==		==About this Structure==
-	~~3IIW~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IIW OCA].	+	[[3iiw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IIW OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~19767730~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019767730</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Gamblin, S J.]]		[[Category: Gamblin, S J.]]
Line 32:		Line 16:
	[[Category: Taylor, W R.]]		[[Category: Taylor, W R.]]
	[[Category: Alternative initiation]]		[[Category: Alternative initiation]]
-	[[Category: Alternative splicing]]
	[[Category: Chromatin regulator]]		[[Category: Chromatin regulator]]
	[[Category: Gene regulation]]		[[Category: Gene regulation]]
Line 42:		Line 25:
	[[Category: Wd repeat]]		[[Category: Wd repeat]]
	[[Category: Wd40 domain]]		[[Category: Wd40 domain]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct 21 09:47:32 2009''

OCA at 07:47, 21 October 2009

OCA — Wed, 21 Oct 2009 07:47:32 GMT

←Older revision		Revision as of 07:47, 21 October 2009
Line 1:		Line 1:
	{{Seed}}		{{Seed}}
-	[[Image:3iiw.~~jpg~~\|left\|200px]]	+	[[Image:3iiw.png\|left\|200px]]

	<!--		<!--
Line 12:		Line 12:
	===Crystal structure of Eed in complex with a trimethylated histone H3K27 peptide===		===Crystal structure of Eed in complex with a trimethylated histone H3K27 peptide===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_19767730}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 19767730 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_19767730}}

	==About this Structure==		==About this Structure==
	3IIW is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IIW OCA].		3IIW is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IIW OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:19767730</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Gamblin, S J.]]		[[Category: Gamblin, S J.]]
Line 34:		Line 43:
	[[Category: Wd40 domain]]		[[Category: Wd40 domain]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Sep 16~~ 09:12:21 2009''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct 21 09:47:32 2009''

OCA at 06:12, 16 September 2009

OCA — Wed, 16 Sep 2009 06:12:22 GMT

←Older revision		Revision as of 06:12, 16 September 2009
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3iiw.jpg\|left\|200px]]

-	The ~~entry 3iiw~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3iiw", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3iiw\| PDB=3iiw \| SCENE= }}

-	~~Authors: Justin, N., Sharpe, M.L., Martin, S., Taylor, W.R., De Marco, V., Gamblin, S.J.~~	+	===Crystal structure of Eed in complex with a trimethylated histone H3K27 peptide===

-	Description: Crystal structure of Eed in complex with a trimethylated histone H3K27 peptide

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Aug 19 12~~:36:02 2009''	+	==About this Structure==
		+	3IIW is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IIW OCA].
		+	[[Category: Homo sapiens]]
		+	[[Category: Gamblin, S J.]]
		+	[[Category: Justin, N.]]
		+	[[Category: Marco, V De.]]
		+	[[Category: Martin, S.]]
		+	[[Category: Sharpe, M L.]]
		+	[[Category: Taylor, W R.]]
		+	[[Category: Alternative initiation]]
		+	[[Category: Alternative splicing]]
		+	[[Category: Chromatin regulator]]
		+	[[Category: Gene regulation]]
		+	[[Category: Nucleus]]
		+	[[Category: Phosphoprotein]]
		+	[[Category: Repressor]]
		+	[[Category: Transcription]]
		+	[[Category: Transcription regulation]]
		+	[[Category: Wd repeat]]
		+	[[Category: Wd40 domain]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 16 09:12:21 2009''