3io5 - Revision history

OCA at 10:06, 21 February 2024

OCA — Wed, 21 Feb 2024 10:06:07 GMT

←Older revision		Revision as of 10:06, 21 February 2024
Line 3:		Line 3:
	<StructureSection load='3io5' size='340' side='right'caption='[[3io5]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='3io5' size='340' side='right'caption='[[3io5]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3io5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Bpt4 Bpt4~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IO5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IO5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3io5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IO5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IO5 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.4Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~fdsA, UVSX ([https~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 BPT4])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3io5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3io5 OCA], [https://pdbe.org/3io5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3io5 RCSB], [https://www.ebi.ac.uk/pdbsum/3io5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3io5 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3io5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3io5 OCA], [https://pdbe.org/3io5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3io5 RCSB], [https://www.ebi.ac.uk/pdbsum/3io5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3io5 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/UVSX_BPT4 UVSX_BPT4]] Important in genetic recombination, DNA repair, and replication. Possesses pairing and strand-transfer activity. Interacts with dda and gene 32 proteins.	+	[https://www.uniprot.org/uniprot/UVSX_BPT4 UVSX_BPT4] Important in genetic recombination, DNA repair, and replication. Possesses pairing and strand-transfer activity. Interacts with dda and gene 32 proteins.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 20:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3io5 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3io5 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Bacteriophage T4 provides an important model system for studying the mechanism of homologous recombination. We have determined the crystal structure of the T4 UvsX recombinase, and the overall architecture and fold closely resemble those of RecA, including a highly conserved ATP binding site. Based on this new structure, we reanalyzed electron microscopy reconstructions of UvsX-DNA filaments and docked the UvsX crystal structure into two different filament forms: a compressed filament generated in the presence of ADP and an elongated filament generated in the presence of ATP and aluminum fluoride. In these reconstructions, the ATP binding site sits at the protomer interface, as in the RecA filament crystal structure. However, the environment of the ATP binding site is altered in the two filament reconstructions, suggesting that nucleotide cannot be as easily accommodated at the protomer interface of the compressed filament. Finally, we show that the phage helicase UvsW completes the UvsX-promoted strand-exchange reaction, allowing the generation of a simple nicked circular product rather than complex networks of partially exchanged substrates.
-
-	Crystal Structure of the Phage T4 Recombinase UvsX and Its Functional Interaction with the T4 SF2 Helicase UvsW.,Gajewski S, Webb MR, Galkin V, Egelman EH, Kreuzer KN, White SW J Mol Biol. 2011 Jan 7;405(1):65-76. Epub 2010 Oct 28. PMID:21035462<ref>PMID:21035462</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3io5" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bpt4~~]]	+	[[Category: Escherichia virus T4]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Gajewski, S]]	+	[[Category: Gajewski S]]
-	~~[[Category: Atp-binding]]~~	+
-	~~[[Category: Dna binding protein]]~~	+
-	~~[[Category: Dna damage]]~~	+
-	~~[[Category: Dna recombination]]~~	+
-	~~[[Category: Dna repair]]~~	+
-	~~[[Category: Dna replication]]~~	+
-	~~[[Category: Inactive conformation]]~~	+
-	~~[[Category: Nucleotide-binding]]~~	+
-	~~[[Category: Reca like core domain]]~~	+
-	~~[[Category: Storage dimer~~]]	+

OCA at 03:37, 21 April 2022

OCA — Thu, 21 Apr 2022 03:37:34 GMT

←Older revision		Revision as of 03:37, 21 April 2022
Line 1:		Line 1:

	==Crystal Structure of a dimeric form of the uvsX Recombinase core domain from Enterobacteria Phage T4==		==Crystal Structure of a dimeric form of the uvsX Recombinase core domain from Enterobacteria Phage T4==
-	<StructureSection load='3io5' size='340' side='right' caption='[[3io5]], [[Resolution\|resolution]] 2.40Å' scene=''>	+	<StructureSection load='3io5' size='340' side='right'caption='[[3io5]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3io5]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Bpt4 Bpt4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IO5 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3IO5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3io5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bpt4 Bpt4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IO5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IO5 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fdsA, UVSX ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 BPT4])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fdsA, UVSX ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 BPT4])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3io5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3io5 OCA], [~~http~~://pdbe.org/3io5 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3io5 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3io5 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3io5 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3io5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3io5 OCA], [https://pdbe.org/3io5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3io5 RCSB], [https://www.ebi.ac.uk/pdbsum/3io5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3io5 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/UVSX_BPT4 UVSX_BPT4]] Important in genetic recombination, DNA repair, and replication. Possesses pairing and strand-transfer activity. Interacts with dda and gene 32 proteins.	+	[[https://www.uniprot.org/uniprot/UVSX_BPT4 UVSX_BPT4]] Important in genetic recombination, DNA repair, and replication. Possesses pairing and strand-transfer activity. Interacts with dda and gene 32 proteins.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/io/3io5_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/io/3io5_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 34:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Bpt4]]		[[Category: Bpt4]]
		+	[[Category: Large Structures]]
	[[Category: Gajewski, S]]		[[Category: Gajewski, S]]
	[[Category: Atp-binding]]		[[Category: Atp-binding]]

OCA at 15:14, 4 August 2016

OCA — Thu, 04 Aug 2016 15:14:29 GMT

←Older revision		Revision as of 15:14, 4 August 2016
Line 1:		Line 1:
		+
	==Crystal Structure of a dimeric form of the uvsX Recombinase core domain from Enterobacteria Phage T4==		==Crystal Structure of a dimeric form of the uvsX Recombinase core domain from Enterobacteria Phage T4==
	<StructureSection load='3io5' size='340' side='right' caption='[[3io5]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='3io5' size='340' side='right' caption='[[3io5]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3io5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Enterobacteria_phage_t4 Enterobacteria phage t4~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IO5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3IO5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3io5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpt4 Bpt4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IO5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3IO5 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fdsA, UVSX ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 ~~Enterobacteria phage T4~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fdsA, UVSX ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 BPT4])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3io5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3io5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3io5 RCSB], [http://www.ebi.ac.uk/pdbsum/3io5 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3io5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3io5 OCA], [http://pdbe.org/3io5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3io5 RCSB], [http://www.ebi.ac.uk/pdbsum/3io5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3io5 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3io5 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 27:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3io5" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Enterobacteria phage t4~~]]	+	[[Category: Bpt4]]
	[[Category: Gajewski, S]]		[[Category: Gajewski, S]]
	[[Category: Atp-binding]]		[[Category: Atp-binding]]

OCA at 16:15, 24 December 2014

OCA — Wed, 24 Dec 2014 16:15:37 GMT

←Older revision		Revision as of 16:15, 24 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3io5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3io5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3io5 RCSB], [http://www.ebi.ac.uk/pdbsum/3io5 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3io5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3io5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3io5 RCSB], [http://www.ebi.ac.uk/pdbsum/3io5 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/UVSX_BPT4 UVSX_BPT4]] Important in genetic recombination, DNA repair, and replication. Possesses pairing and strand-transfer activity. Interacts with dda and gene 32 proteins.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 17:07, 18 December 2014

OCA — Thu, 18 Dec 2014 17:07:07 GMT

←Older revision		Revision as of 17:07, 18 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3io5\| PDB=3io5 \| SCENE= }}~~	+	==Crystal Structure of a dimeric form of the uvsX Recombinase core domain from Enterobacteria Phage T4==
-	===Crystal Structure of a dimeric form of the uvsX Recombinase core domain from Enterobacteria Phage T4===	+	<StructureSection load='3io5' size='340' side='right' caption='[[3io5]], [[Resolution\|resolution]] 2.40Å' scene=''>
-	~~{{ABSTRACT_PUBMED_21035462}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3io5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t4 Enterobacteria phage t4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IO5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3IO5 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fdsA, UVSX ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 Enterobacteria phage T4])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3io5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3io5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3io5 RCSB], [http://www.ebi.ac.uk/pdbsum/3io5 PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/io/3io5_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bacteriophage T4 provides an important model system for studying the mechanism of homologous recombination. We have determined the crystal structure of the T4 UvsX recombinase, and the overall architecture and fold closely resemble those of RecA, including a highly conserved ATP binding site. Based on this new structure, we reanalyzed electron microscopy reconstructions of UvsX-DNA filaments and docked the UvsX crystal structure into two different filament forms: a compressed filament generated in the presence of ADP and an elongated filament generated in the presence of ATP and aluminum fluoride. In these reconstructions, the ATP binding site sits at the protomer interface, as in the RecA filament crystal structure. However, the environment of the ATP binding site is altered in the two filament reconstructions, suggesting that nucleotide cannot be as easily accommodated at the protomer interface of the compressed filament. Finally, we show that the phage helicase UvsW completes the UvsX-promoted strand-exchange reaction, allowing the generation of a simple nicked circular product rather than complex networks of partially exchanged substrates.

-	~~==About this~~ Structure==	+	Crystal Structure of the Phage T4 Recombinase UvsX and Its Functional Interaction with the T4 SF2 Helicase UvsW.,Gajewski S, Webb MR, Galkin V, Egelman EH, Kreuzer KN, White SW J Mol Biol. 2011 Jan 7;405(1):65-76. Epub 2010 Oct 28. PMID:21035462<ref>PMID:21035462</ref>
-	~~[[3io5]] is a 2 chain structure~~ with ~~sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Enterobacteria_phage_t4 Enterobacteria phage t4]. Full crystallographic information is available from [http~~:~~//oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=3IO5 OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:021035462</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Enterobacteria phage t4]]		[[Category: Enterobacteria phage t4]]
-	[[Category: Gajewski, S.]]	+	[[Category: Gajewski, S]]
	[[Category: Atp-binding]]		[[Category: Atp-binding]]
	[[Category: Dna binding protein]]		[[Category: Dna binding protein]]

OCA at 09:22, 27 March 2013

OCA — Wed, 27 Mar 2013 09:22:14 GMT

←Older revision		Revision as of 09:22, 27 March 2013
Line 1:		Line 1:
-	[[Image:3io5.png\|left\|200px]]
-
-	<!--
-	The line below this paragraph, containing "STRUCTURE_3io5", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3io5\| PDB=3io5 \| SCENE= }}		{{STRUCTURE_3io5\| PDB=3io5 \| SCENE= }}
-
	===Crystal Structure of a dimeric form of the uvsX Recombinase core domain from Enterobacteria Phage T4===		===Crystal Structure of a dimeric form of the uvsX Recombinase core domain from Enterobacteria Phage T4===
-
-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_21035462}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 21035462 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_21035462}}		{{ABSTRACT_PUBMED_21035462}}

Line 22:		Line 7:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~21035462~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:021035462</ref><references group="xtra"/><references/>
	[[Category: Enterobacteria phage t4]]		[[Category: Enterobacteria phage t4]]
	[[Category: Gajewski, S.]]		[[Category: Gajewski, S.]]
		+	[[Category: Atp-binding]]
		+	[[Category: Dna binding protein]]
		+	[[Category: Dna damage]]
		+	[[Category: Dna recombination]]
		+	[[Category: Dna repair]]
		+	[[Category: Dna replication]]
		+	[[Category: Inactive conformation]]
		+	[[Category: Nucleotide-binding]]
		+	[[Category: Reca like core domain]]
		+	[[Category: Storage dimer]]

OCA: Protected "3io5" [edit=sysop:move=sysop]

OCA — Wed, 05 Jan 2011 15:42:15 GMT

Protected "3io5" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:42, 5 January 2011

OCA at 15:42, 5 January 2011

OCA — Wed, 05 Jan 2011 15:42:13 GMT

←Older revision		Revision as of 15:42, 5 January 2011
Line 1:		Line 1:
-	~~{{Seed}}~~	+	[[Image:3io5.png\|left\|200px]]
-	[[Image:3io5.~~jpg~~\|left\|200px]]	+

	<!--		<!--
Line 12:		Line 11:
	===Crystal Structure of a dimeric form of the uvsX Recombinase core domain from Enterobacteria Phage T4===		===Crystal Structure of a dimeric form of the uvsX Recombinase core domain from Enterobacteria Phage T4===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21035462}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21035462 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21035462}}

	==About this Structure==		==About this Structure==
-	~~3IO5~~ is a 2 ~~chains~~ structure with ~~sequences~~ from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t4 Enterobacteria phage t4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IO5 OCA].	+	[[3io5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t4 Enterobacteria phage t4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IO5 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:21035462</ref><references group="xtra"/>
	[[Category: Enterobacteria phage t4]]		[[Category: Enterobacteria phage t4]]
	[[Category: Gajewski, S.]]		[[Category: Gajewski, S.]]
-	[[Category: Atp-binding]]
-	[[Category: Dna binding protein]]
-	[[Category: Dna damage]]
-	[[Category: Dna recombination]]
-	[[Category: Dna repair]]
-	[[Category: Dna replication]]
-	[[Category: Inactive conformation]]
-	[[Category: Nucleotide-binding]]
-	[[Category: Reca like core domain]]
-	[[Category: Storage dimer]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug 25 08:54:51 2010''

OCA at 04:47, 25 August 2010

OCA — Wed, 25 Aug 2010 04:47:02 GMT

←Older revision		Revision as of 04:47, 25 August 2010
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3io5.jpg\|left\|200px]]

-	The ~~entry 3io5~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3io5", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3io5\| PDB=3io5 \| SCENE= }}

-	~~Authors: Gajewski, S.~~	+	===Crystal Structure of a dimeric form of the uvsX Recombinase core domain from Enterobacteria Phage T4===

-	Description: Crystal Structure of a dimeric form of the uvsX Recombinase core domain from Enterobacteria Phage T4

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug 4 08:43:13 2010''	+	==About this Structure==
		+	3IO5 is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t4 Enterobacteria phage t4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IO5 OCA].
		+	[[Category: Enterobacteria phage t4]]
		+	[[Category: Gajewski, S.]]
		+	[[Category: Atp-binding]]
		+	[[Category: Dna binding protein]]
		+	[[Category: Dna damage]]
		+	[[Category: Dna recombination]]
		+	[[Category: Dna repair]]
		+	[[Category: Dna replication]]
		+	[[Category: Inactive conformation]]
		+	[[Category: Nucleotide-binding]]
		+	[[Category: Reca like core domain]]
		+	[[Category: Storage dimer]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug 25 08:54:51 2010''

OCA at 04:36, 4 August 2010

OCA — Wed, 04 Aug 2010 04:36:14 GMT

←Older revision		Revision as of 04:36, 4 August 2010
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3io5 is ON HOLD until ~~sometime in the future~~	+	The entry 3io5 is ON HOLD until Paper Publication

	Authors: Gajewski, S.		Authors: Gajewski, S.
Line 7:		Line 7:
	Description: Crystal Structure of a dimeric form of the uvsX Recombinase core domain from Enterobacteria Phage T4		Description: Crystal Structure of a dimeric form of the uvsX Recombinase core domain from Enterobacteria Phage T4

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Jul 21 10~~:00:00 2010''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug 4 08:43:13 2010''