3iqm - Revision history

OCA at 07:56, 6 September 2023

OCA — Wed, 06 Sep 2023 07:56:25 GMT

←Older revision		Revision as of 07:56, 6 September 2023
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	<StructureSection load='3iqm' size='340' side='right'caption='[[3iqm]], [[Resolution\|resolution]] 3.40Å' scene=''>		<StructureSection load='3iqm' size='340' side='right'caption='[[3iqm]], [[Resolution\|resolution]] 3.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3iqm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Bacsu Bacsu~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IQM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IQM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3iqm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IQM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IQM FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.4Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'~~>~~[[3iqy\|3iqy]]~~<~~/div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">BSU35300, div+, secA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 BACSU])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3iqm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3iqm OCA], [https://pdbe.org/3iqm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3iqm RCSB], [https://www.ebi.ac.uk/pdbsum/3iqm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3iqm ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3iqm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3iqm OCA], [https://pdbe.org/3iqm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3iqm RCSB], [https://www.ebi.ac.uk/pdbsum/3iqm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3iqm ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/SECA_BACSU SECA_BACSU]] Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. Has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving as an ATP-driven molecular motor driving the stepwise translocation of polypeptide chains across the membrane (By similarity).[HAMAP-Rule:MF_01382]	+	[https://www.uniprot.org/uniprot/SECA_BACSU SECA_BACSU] Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. Has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving as an ATP-driven molecular motor driving the stepwise translocation of polypeptide chains across the membrane (By similarity).[HAMAP-Rule:MF_01382]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 38:		Line 37:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacsu~~]]	+	[[Category: Bacillus subtilis subsp. subtilis str. 168]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Hunt~~, J F~~]]	+	[[Category: Hunt JF]]
-	[[Category: Kim, D]]	+	[[Category: Kim D]]
-	~~[[Category: Alpha-beta protein]]~~	+
-	~~[[Category: Atp-binding]]~~	+
-	~~[[Category: Cell membrane]]~~	+
-	~~[[Category: Membrane]]~~	+
-	~~[[Category: Metal-binding]]~~	+
-	~~[[Category: Nucleotide-binding]]~~	+
-	~~[[Category: Protein transport]]~~	+
-	~~[[Category: Translocation]]~~	+
-	~~[[Category: Transport~~]]	+

OCA at 12:14, 13 October 2021

OCA — Wed, 13 Oct 2021 12:14:12 GMT

←Older revision		Revision as of 12:14, 13 October 2021
Line 1:		Line 1:

	==Active site mutants of B. subtilis SecA==		==Active site mutants of B. subtilis SecA==
-	<StructureSection load='3iqm' size='340' side='right' caption='[[3iqm]], [[Resolution\|resolution]] 3.40Å' scene=''>	+	<StructureSection load='3iqm' size='340' side='right'caption='[[3iqm]], [[Resolution\|resolution]] 3.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3iqm]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Bacsu Bacsu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IQM OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3IQM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3iqm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacsu Bacsu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IQM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IQM FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3iqy\|3iqy]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3iqy\|3iqy]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BSU35300, div+, secA ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 BACSU])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BSU35300, div+, secA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 BACSU])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3iqm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3iqm OCA], [~~http~~://pdbe.org/3iqm PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3iqm RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3iqm PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3iqm ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3iqm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3iqm OCA], [https://pdbe.org/3iqm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3iqm RCSB], [https://www.ebi.ac.uk/pdbsum/3iqm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3iqm ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/SECA_BACSU SECA_BACSU]] Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. Has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving as an ATP-driven molecular motor driving the stepwise translocation of polypeptide chains across the membrane (By similarity).[HAMAP-Rule:MF_01382]	+	[[https://www.uniprot.org/uniprot/SECA_BACSU SECA_BACSU]] Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. Has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving as an ATP-driven molecular motor driving the stepwise translocation of polypeptide chains across the membrane (By similarity).[HAMAP-Rule:MF_01382]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 39:		Line 39:
	</StructureSection>		</StructureSection>
	[[Category: Bacsu]]		[[Category: Bacsu]]
		+	[[Category: Large Structures]]
	[[Category: Hunt, J F]]		[[Category: Hunt, J F]]
	[[Category: Kim, D]]		[[Category: Kim, D]]

OCA at 09:38, 19 December 2018

OCA — Wed, 19 Dec 2018 09:38:54 GMT

←Older revision		Revision as of 09:38, 19 December 2018
Line 15:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/iq/3iqm_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/iq/3iqm_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 20:07, 5 August 2016

OCA — Fri, 05 Aug 2016 20:07:32 GMT

←Older revision		Revision as of 20:07, 5 August 2016
Line 1:		Line 1:
		+
	==Active site mutants of B. subtilis SecA==		==Active site mutants of B. subtilis SecA==
	<StructureSection load='3iqm' size='340' side='right' caption='[[3iqm]], [[Resolution\|resolution]] 3.40Å' scene=''>		<StructureSection load='3iqm' size='340' side='right' caption='[[3iqm]], [[Resolution\|resolution]] 3.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3iqm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IQM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3IQM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3iqm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacsu Bacsu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IQM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3IQM FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3iqy\|3iqy]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3iqy\|3iqy]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BSU35300, div+, secA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 ~~Bacillus subtilis subsp. subtilis str. 168~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BSU35300, div+, secA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 BACSU])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3iqm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3iqm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3iqm RCSB], [http://www.ebi.ac.uk/pdbsum/3iqm PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3iqm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3iqm OCA], [http://pdbe.org/3iqm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3iqm RCSB], [http://www.ebi.ac.uk/pdbsum/3iqm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3iqm ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3iqm ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 28:		Line 29:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3iqm" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 36:		Line 38:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus subtilis subsp. subtilis str. 168~~]]	+	[[Category: Bacsu]]
	[[Category: Hunt, J F]]		[[Category: Hunt, J F]]
	[[Category: Kim, D]]		[[Category: Kim, D]]

OCA at 21:32, 25 December 2014

OCA — Thu, 25 Dec 2014 21:32:11 GMT

←Older revision		Revision as of 21:32, 25 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3iqm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3iqm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3iqm RCSB], [http://www.ebi.ac.uk/pdbsum/3iqm PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3iqm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3iqm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3iqm RCSB], [http://www.ebi.ac.uk/pdbsum/3iqm PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SECA_BACSU SECA_BACSU]] Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. Has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving as an ATP-driven molecular motor driving the stepwise translocation of polypeptide chains across the membrane (By similarity).[HAMAP-Rule:MF_01382]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 16:05, 18 December 2014

OCA — Thu, 18 Dec 2014 16:05:04 GMT

←Older revision		Revision as of 16:05, 18 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3iqm~~\| ~~PDB~~=3iqm \| ~~SCENE~~= }}	+	==Active site mutants of B. subtilis SecA==
-	===~~Active site mutants of B~~. subtilis ~~SecA~~===	+	<StructureSection load='3iqm' size='340' side='right' caption='[[3iqm]], [[Resolution\|resolution]] 3.40Å' scene=''>
-	~~{{ABSTRACT_PUBMED_23167435}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3iqm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IQM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3IQM FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3iqy\|3iqy]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BSU35300, div+, secA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 Bacillus subtilis subsp. subtilis str. 168])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3iqm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3iqm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3iqm RCSB], [http://www.ebi.ac.uk/pdbsum/3iqm PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/iq/3iqm_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	SecA is an intensively studied mechanoenzyme that uses ATP hydrolysis to drive processive extrusion of secreted proteins through a protein-conducting channel in the cytoplasmic membrane of eubacteria. The ATPase motor of SecA is strongly homologous to that in DEAD-box RNA helicases. It remains unclear how local chemical events in its ATPase active site control the overall conformation of an approximately 100 kDa multidomain enzyme and drive protein transport. In this paper, we use biophysical methods to establish that a single electrostatic charge in the ATPase active site controls the global conformation of SecA. The enzyme undergoes an ATP-modulated endothermic conformational transition (ECT) believed to involve similar structural mechanics to the protein transport reaction. We have characterized the effects of an isosteric glutamate-to-glutamine mutation in the catalytic base, a mutation which mimics the immediate electrostatic consequences of ATP hydrolysis in the active site. Calorimetric studies demonstrate that this mutation facilitates the ECT in Escherichia coli SecA and triggers it completely in Bacillus subtilis SecA. Consistent with the substantial increase in entropy observed in the course of the ECT, hydrogen-deuterium exchange mass spectrometry demonstrates that it increases protein backbone dynamics in domain-domain interfaces at remote locations from the ATPase active site. The catalytic glutamate is one of approximately 250 charged amino acids in SecA, and yet neutralization of its side chain charge is sufficient to trigger a global order-disorder transition in this 100 kDa enzyme. The intricate network of structural interactions mediating this effect couples local electrostatic changes during ATP hydrolysis to global conformational and dynamic changes in SecA. This network forms the foundation of the allosteric mechanochemistry that efficiently harnesses the chemical energy stored in ATP to drive complex mechanical processes.

-	~~==Function==~~	+	ATPase Active-Site Electrostatic Interactions Control the Global Conformation of the 100 kDa SecA Translocase.,Kim DM, Zheng H, Huang YJ, Montelione GT, Hunt JF J Am Chem Soc. 2013 Feb 27;135(8):2999-3010. doi: 10.1021/ja306361q. Epub 2013, Feb 14. PMID:23167435<ref>PMID:23167435</ref>
-	~~[[http://www.uniprot.org/uniprot/SECA_BACSU SECA_BACSU]] Part~~ of the ~~Sec protein translocase complex~~. ~~Interacts with the SecYEG preprotein conducting channel~~. Has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving as an ATP-driven molecular motor driving the stepwise translocation of polypeptide chains across the membrane (~~By similarity~~).~~[HAMAP-Rule~~:~~MF_01382]~~	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[3iqm]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Bacillus_subtilis_subsp~~.~~_subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3IQM OCA].~~	+	</div>

	==See Also==		==See Also==
		+	*[[Preprotein translocase\|Preprotein translocase]]
	*[[SecA\|SecA]]		*[[SecA\|SecA]]
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Bacillus subtilis subsp. subtilis str. 168]]		[[Category: Bacillus subtilis subsp. subtilis str. 168]]
-	[[Category: Hunt, J F.]]	+	[[Category: Hunt, J F]]
-	[[Category: Kim, D.]]	+	[[Category: Kim, D]]
	[[Category: Alpha-beta protein]]		[[Category: Alpha-beta protein]]
	[[Category: Atp-binding]]		[[Category: Atp-binding]]

OCA at 23:58, 3 April 2013

OCA — Wed, 03 Apr 2013 23:58:45 GMT

←Older revision		Revision as of 23:58, 3 April 2013
Line 1:		Line 1:
-	[[Image:3iqm.png\|left\|200px]]
-
	{{STRUCTURE_3iqm\| PDB=3iqm \| SCENE= }}		{{STRUCTURE_3iqm\| PDB=3iqm \| SCENE= }}
-
	===Active site mutants of B. subtilis SecA===		===Active site mutants of B. subtilis SecA===
		+	{{ABSTRACT_PUBMED_23167435}}

-	~~{{ABSTRACT_PUBMED_23167435}}~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/SECA_BACSU SECA_BACSU]] Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. Has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving as an ATP-driven molecular motor driving the stepwise translocation of polypeptide chains across the membrane (By similarity).[HAMAP-Rule:MF_01382]

	==About this Structure==		==About this Structure==

OCA at 13:18, 6 March 2013

OCA — Wed, 06 Mar 2013 13:18:31 GMT

←Older revision		Revision as of 13:18, 6 March 2013
Line 5:		Line 5:
	===Active site mutants of B. subtilis SecA===		===Active site mutants of B. subtilis SecA===

		+	{{ABSTRACT_PUBMED_23167435}}

	==About this Structure==		==About this Structure==
-	[[3iqm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis_subsp.~~_subtilis~~ Bacillus subtilis subsp. subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IQM OCA].	+	[[3iqm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IQM OCA].

	==See Also==		==See Also==
	*[[SecA\|SecA]]		*[[SecA\|SecA]]
-	[[Category: Bacillus subtilis subsp. subtilis]]	+	[[Category: Bacillus subtilis subsp. subtilis str. 168]]
	[[Category: Hunt, J F.]]		[[Category: Hunt, J F.]]
	[[Category: Kim, D.]]		[[Category: Kim, D.]]

OCA at 21:54, 20 October 2012

OCA — Sat, 20 Oct 2012 21:54:27 GMT

←Older revision		Revision as of 21:54, 20 October 2012
Line 7:		Line 7:

	==About this Structure==		==About this Structure==
-	[[3iqm]] is a 1 chain structure ~~of [[SecA]]~~ with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis Bacillus subtilis subsp. subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IQM OCA].	+	[[3iqm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis Bacillus subtilis subsp. subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IQM OCA].

	==See Also==		==See Also==

OCA: Protected "3iqm" [edit=sysop:move=sysop]

OCA — Fri, 27 Jul 2012 14:37:30 GMT

Protected "3iqm" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:37, 27 July 2012