3iva - Revision history

OCA at 07:58, 6 September 2023

OCA — Wed, 06 Sep 2023 07:58:45 GMT

←Older revision		Revision as of 07:58, 6 September 2023
Line 3:		Line 3:
	<StructureSection load='3iva' size='340' side='right'caption='[[3iva]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='3iva' size='340' side='right'caption='[[3iva]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3iva]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IVA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IVA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3iva]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IVA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IVA FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=B12:COBALAMIN'>B12</scene>~~, ~~<scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.7Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>~~[[~~Related_structure~~\|~~Related:~~]]~~</b></td><td class="sblockDat"><div style='overflow: auto~~; ~~max-height: 3em;'>[[1k7y\|1k7y]], [[3bul\|3bul]], [[3iv9\|3iv9]]</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=B12:COBALAMIN'>B12</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>b4019, JW3979, metH ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~562~~ "~~Bacillus coli~~" ~~Migula 1895])</td~~><~~/tr>~~	+
-	~~<tr id~~='~~activity~~'><~~td class="sblockLbl"~~><~~b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Methionine_synthase Methionine synthase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.13 2.1.1.13]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3iva FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3iva OCA], [https://pdbe.org/3iva PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3iva RCSB], [https://www.ebi.ac.uk/pdbsum/3iva PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3iva ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3iva FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3iva OCA], [https://pdbe.org/3iva PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3iva RCSB], [https://www.ebi.ac.uk/pdbsum/3iva PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3iva ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/METH_ECOLI METH_ECOLI]] Catalyzes the transfer of a methyl group from methyl-cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate.	+	[https://www.uniprot.org/uniprot/METH_ECOLI METH_ECOLI] Catalyzes the transfer of a methyl group from methyl-cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</div>		</div>
	<div class="pdbe-citations 3iva" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3iva" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Methionine synthase 3D structures\|Methionine synthase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895~~]]	+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Methionine synthase]]~~	+	[[Category: Koutmos M]]
-	[[Category: Koutmos, M]]	+	[[Category: Pattridge KA]]
-	[[Category: Pattridge~~, K A~~]]	+	[[Category: Smith JL]]
-	[[Category: Smith~~, J L]]~~	+
-	~~[[Category: Amino-acid biosynthesis]]~~	+
-	~~[[Category: Cobalamin]]~~	+
-	~~[[Category: Cobalt]]~~	+
-	~~[[Category: H759]]~~	+
-	~~[[Category: Intermodular interaction]]~~	+
-	~~[[Category: Metal-binding]]~~	+
-	~~[[Category: Meth]]~~	+
-	~~[[Category: Methionine biosynthesis]]~~	+
-	~~[[Category: Methyltransferase]]~~	+
-	~~[[Category: Reactivation conformation]]~~	+
-	~~[[Category: S-adenosyl-homocysteine]]~~	+
-	~~[[Category: S-adenosyl-l-methionine]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 12:15, 13 October 2021

OCA — Wed, 13 Oct 2021 12:15:42 GMT

←Older revision		Revision as of 12:15, 13 October 2021
Line 1:		Line 1:

	==Structure of the B12-dependent Methionine Synthase (MetH) C-teminal half with AdoHcy bound==		==Structure of the B12-dependent Methionine Synthase (MetH) C-teminal half with AdoHcy bound==
-	<StructureSection load='3iva' size='340' side='right' caption='[[3iva]], [[Resolution\|resolution]] 2.70Å' scene=''>	+	<StructureSection load='3iva' size='340' side='right'caption='[[3iva]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3iva]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IVA OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3IVA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3iva]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IVA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IVA FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=B12:COBALAMIN'>B12</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=B12:COBALAMIN'>B12</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1k7y\|1k7y]], [[3bul\|3bul]], [[3iv9\|3iv9]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1k7y\|1k7y]], [[3bul\|3bul]], [[3iv9\|3iv9]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b4019, JW3979, metH ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b4019, JW3979, metH ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Methionine_synthase Methionine synthase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.13 2.1.1.13] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Methionine_synthase Methionine synthase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.13 2.1.1.13] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3iva FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3iva OCA], [~~http~~://pdbe.org/3iva PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3iva RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3iva PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3iva ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3iva FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3iva OCA], [https://pdbe.org/3iva PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3iva RCSB], [https://www.ebi.ac.uk/pdbsum/3iva PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3iva ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/METH_ECOLI METH_ECOLI]] Catalyzes the transfer of a methyl group from methyl-cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate.	+	[[https://www.uniprot.org/uniprot/METH_ECOLI METH_ECOLI]] Catalyzes the transfer of a methyl group from methyl-cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
		+	[[Category: Large Structures]]
	[[Category: Methionine synthase]]		[[Category: Methionine synthase]]
	[[Category: Koutmos, M]]		[[Category: Koutmos, M]]

OCA at 09:45, 19 December 2018

OCA — Wed, 19 Dec 2018 09:45:12 GMT

←Older revision		Revision as of 09:45, 19 December 2018
Line 1:		Line 1:
		+
	==Structure of the B12-dependent Methionine Synthase (MetH) C-teminal half with AdoHcy bound==		==Structure of the B12-dependent Methionine Synthase (MetH) C-teminal half with AdoHcy bound==
	<StructureSection load='3iva' size='340' side='right' caption='[[3iva]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='3iva' size='340' side='right' caption='[[3iva]], [[Resolution\|resolution]] 2.70Å' scene=''>
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	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b4019, JW3979, metH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b4019, JW3979, metH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Methionine_synthase Methionine synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.13 2.1.1.13] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Methionine_synthase Methionine synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.13 2.1.1.13] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3iva FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3iva OCA], [http://pdbe.org/3iva PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3iva RCSB], [http://www.ebi.ac.uk/pdbsum/3iva PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3iva FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3iva OCA], [http://pdbe.org/3iva PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3iva RCSB], [http://www.ebi.ac.uk/pdbsum/3iva PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3iva ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/iv/3iva_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/iv/3iva_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 06:09, 8 February 2016

OCA — Mon, 08 Feb 2016 06:09:25 GMT

←Older revision		Revision as of 06:09, 8 February 2016
Line 2:		Line 2:
	<StructureSection load='3iva' size='340' side='right' caption='[[3iva]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='3iva' size='340' side='right' caption='[[3iva]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3iva]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IVA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3IVA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3iva]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IVA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3IVA FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=B12:COBALAMIN'>B12</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=B12:COBALAMIN'>B12</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1k7y\|1k7y]], [[3bul\|3bul]], [[3iv9\|3iv9]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1k7y\|1k7y]], [[3bul\|3bul]], [[3iv9\|3iv9]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b4019, JW3979, metH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 ~~Escherichia~~ coli])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b4019, JW3979, metH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Methionine_synthase Methionine synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.13 2.1.1.13] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Methionine_synthase Methionine synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.13 2.1.1.13] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3iva FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3iva OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3iva RCSB], [http://www.ebi.ac.uk/pdbsum/3iva PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3iva FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3iva OCA], [http://pdbe.org/3iva PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3iva RCSB], [http://www.ebi.ac.uk/pdbsum/3iva PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 19:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3iva ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3iva" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia~~ coli]]	+	[[Category: Bacillus coli migula 1895]]
	[[Category: Methionine synthase]]		[[Category: Methionine synthase]]
	[[Category: Koutmos, M]]		[[Category: Koutmos, M]]

OCA at 21:12, 24 December 2014

OCA — Wed, 24 Dec 2014 21:12:21 GMT

←Older revision		Revision as of 21:12, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3iva]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IVA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3IVA FirstGlance]. <br>		<table><tr><td colspan='2'>[[3iva]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IVA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3IVA FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=B12:COBALAMIN'>B12</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=B12:COBALAMIN'>B12</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1k7y\|1k7y]], [[3bul\|3bul]], [[3iv9\|3iv9]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1k7y\|1k7y]], [[3bul\|3bul]], [[3iv9\|3iv9]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b4019, JW3979, metH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b4019, JW3979, metH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Methionine_synthase Methionine synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.13 2.1.1.13] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Methionine_synthase Methionine synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.13 2.1.1.13] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3iva FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3iva OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3iva RCSB], [http://www.ebi.ac.uk/pdbsum/3iva PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3iva FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3iva OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3iva RCSB], [http://www.ebi.ac.uk/pdbsum/3iva PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/METH_ECOLI METH_ECOLI]] Catalyzes the transfer of a methyl group from methyl-cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 35:
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Methionine synthase]]		[[Category: Methionine synthase]]
-	[[Category: Koutmos, M.]]	+	[[Category: Koutmos, M]]
-	[[Category: Pattridge, K A.]]	+	[[Category: Pattridge, K A]]
-	[[Category: Smith, J L.]]	+	[[Category: Smith, J L]]
	[[Category: Amino-acid biosynthesis]]		[[Category: Amino-acid biosynthesis]]
	[[Category: Cobalamin]]		[[Category: Cobalamin]]

OCA at 12:26, 29 September 2014

OCA — Mon, 29 Sep 2014 12:26:33 GMT

←Older revision		Revision as of 12:26, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~3iva.~~png~~\|~~left~~\|200px]]	+	==Structure of the B12-dependent Methionine Synthase (MetH) C-teminal half with AdoHcy bound==
		+	<StructureSection load='3iva' size='340' side='right' caption='[[3iva]], [[Resolution\|resolution]] 2.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3iva]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IVA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3IVA FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=B12:COBALAMIN'>B12</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1k7y\|1k7y]], [[3bul\|3bul]], [[3iv9\|3iv9]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b4019, JW3979, metH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Methionine_synthase Methionine synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.13 2.1.1.13] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3iva FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3iva OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3iva RCSB], [http://www.ebi.ac.uk/pdbsum/3iva PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/iv/3iva_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cobalamin-dependent methionine synthase (MetH) is a modular protein that catalyzes the transfer of a methyl group from methyltetrahydrofolate to homocysteine to produce methionine and tetrahydrofolate. The cobalamin cofactor, which serves as both acceptor and donor of the methyl group, is oxidized once every approximately 2,000 catalytic cycles and must be reactivated by the uptake of an electron from reduced flavodoxin and a methyl group from S-adenosyl-L-methionine (AdoMet). Previous structures of a C-terminal fragment of MetH (MetH(CT)) revealed a reactivation conformation that juxtaposes the cobalamin- and AdoMet-binding domains. Here we describe 2 structures of a disulfide stabilized MetH(CT) ((s-s)MetH(CT)) that offer further insight into the reactivation of MetH. The structure of (s-s)MetH(CT) with cob(II)alamin and S-adenosyl-L-homocysteine represents the enzyme in the reactivation step preceding electron transfer from flavodoxin. The structure supports earlier suggestions that the enzyme acts to lower the reduction potential of the Co(II)/Co(I) couple by elongating the bond between the cobalt and its upper axial water ligand, effectively making the cobalt 4-coordinate, and illuminates the role of Tyr-1139 in the stabilization of this 4-coordinate state. The structure of (s-s)MetH(CT) with aquocobalamin may represent a transient state at the end of reactivation as the newly remethylated 5-coordinate methylcobalamin returns to the 6-coordinate state, triggering the rearrangement to a catalytic conformation.

-	~~{{STRUCTURE_3iva\| PDB=3iva \| SCENE= }}~~	+	Insights into the reactivation of cobalamin-dependent methionine synthase.,Koutmos M, Datta S, Pattridge KA, Smith JL, Matthews RG Proc Natl Acad Sci U S A. 2009 Nov 3;106(44):18527-32. Epub 2009 Oct 21. PMID:19846791<ref>PMID:19846791</ref>

-	~~===Structure~~ of the ~~B12-dependent Methionine Synthase (MetH) C-teminal half with AdoHcy bound===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_19846791}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[3iva]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli Escherichia coli]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3IVA OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:019846791<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Methionine synthase]]		[[Category: Methionine synthase]]

OCA: Protected "3iva" [edit=sysop:move=sysop]

OCA — Wed, 24 Oct 2012 06:49:25 GMT

Protected "3iva" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:49, 24 October 2012

OCA at 06:49, 24 October 2012

OCA — Wed, 24 Oct 2012 06:49:23 GMT

←Older revision		Revision as of 06:49, 24 October 2012
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-	{{Seed}}
	[[Image:3iva.png\|left\|200px]]		[[Image:3iva.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3iva", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3iva\| PDB=3iva \| SCENE= }}		{{STRUCTURE_3iva\| PDB=3iva \| SCENE= }}

	===Structure of the B12-dependent Methionine Synthase (MetH) C-teminal half with AdoHcy bound===		===Structure of the B12-dependent Methionine Synthase (MetH) C-teminal half with AdoHcy bound===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_19846791}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 19846791 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_19846791}}		{{ABSTRACT_PUBMED_19846791}}

	==About this Structure==		==About this Structure==
-	~~3IVA~~ is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IVA OCA].	+	[[3iva]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IVA OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~19846791~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019846791</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Methionine synthase]]		[[Category: Methionine synthase]]
Line 42:		Line 30:
	[[Category: S-adenosyl-l-methionine]]		[[Category: S-adenosyl-l-methionine]]
	[[Category: Transferase]]		[[Category: Transferase]]
-	[[Category: Zinc]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jan 28 15:11:35 2010''

OCA at 13:11, 28 January 2010

OCA — Thu, 28 Jan 2010 13:11:36 GMT

←Older revision		Revision as of 13:11, 28 January 2010
Line 1:		Line 1:
	{{Seed}}		{{Seed}}
-	[[Image:3iva.~~jpg~~\|left\|200px]]	+	[[Image:3iva.png\|left\|200px]]

	<!--		<!--
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	3IVA is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IVA OCA].	+	3IVA is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IVA OCA].

	==Reference==		==Reference==
Line 44:		Line 44:
	[[Category: Zinc]]		[[Category: Zinc]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Nov 25 10~~:09:~~04 2009~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jan 28 15:11:35 2010''

OCA at 08:09, 25 November 2009

OCA — Wed, 25 Nov 2009 08:09:05 GMT

←Older revision		Revision as of 08:09, 25 November 2009
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3iva.jpg\|left\|200px]]

-	The ~~entry 3iva~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3iva", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3iva\| PDB=3iva \| SCENE= }}

-	~~Authors: Pattridge, K.A., Koutmos, M., Smith, J.L.~~	+	===Structure of the B12-dependent Methionine Synthase (MetH) C-teminal half with AdoHcy bound===

-	Description: Structure of the B12-dependent Methionine Synthase (MetH) C-teminal half with AdoHcy bound

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Sep 30 08~~:59:21 2009''	+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_19846791}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 19846791 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_19846791}}
		+
		+	==About this Structure==
		+	3IVA is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IVA OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:19846791</ref><references group="xtra"/>
		+	[[Category: Escherichia coli]]
		+	[[Category: Methionine synthase]]
		+	[[Category: Koutmos, M.]]
		+	[[Category: Pattridge, K A.]]
		+	[[Category: Smith, J L.]]
		+	[[Category: Amino-acid biosynthesis]]
		+	[[Category: Cobalamin]]
		+	[[Category: Cobalt]]
		+	[[Category: H759]]
		+	[[Category: Intermodular interaction]]
		+	[[Category: Metal-binding]]
		+	[[Category: Meth]]
		+	[[Category: Methionine biosynthesis]]
		+	[[Category: Methyltransferase]]
		+	[[Category: Reactivation conformation]]
		+	[[Category: S-adenosyl-homocysteine]]
		+	[[Category: S-adenosyl-l-methionine]]
		+	[[Category: Transferase]]
		+	[[Category: Zinc]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Nov 25 10:09:04 2009''