3j67 - Revision history

OCA at 10:10, 21 February 2024

OCA — Wed, 21 Feb 2024 10:10:31 GMT

←Older revision		Revision as of 10:10, 21 February 2024
Line 9:		Line 9:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/DYHC_YEAST DYHC_YEAST] Cytoplasmic dynein acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. Required to maintain uniform nuclear distribution in hyphae. May play an important role in the proper orientation of the mitotic spindle into the budding daughter cell yeast. Probably required for normal progression of the cell cycle.<ref>PMID:15642746</ref>		[https://www.uniprot.org/uniprot/DYHC_YEAST DYHC_YEAST] Cytoplasmic dynein acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. Required to maintain uniform nuclear distribution in hyphae. May play an important role in the proper orientation of the mitotic spindle into the budding daughter cell yeast. Probably required for normal progression of the cell cycle.<ref>PMID:15642746</ref>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Dyneins are large microtubule motor proteins required for mitosis, intracellular transport and ciliary and flagellar motility. They generate force through a power-stroke mechanism, which is an ATP-consuming cycle of pre- and post-power-stroke conformational changes that cause relative motion between different dynein domains. However, key structural details of dynein's force generation remain elusive. Here, using cryo-electron tomography of intact, active (that is, beating), rapidly frozen sea urchin sperm flagella, we determined the in situ three-dimensional structures of all domains of both pre- and post-power-stroke dynein, including the previously unresolved linker and stalk of pre-power-stroke dynein. Our results reveal that the rotation of the head relative to the linker is the key action in dynein movement, and that there are at least two distinct pre-power-stroke conformations: pre-I (microtubule-detached) and pre-II (microtubule-bound). We provide three-dimensional reconstructions of native dyneins in three conformational states, in situ, allowing us to propose a molecular model of the structural cycle underlying dynein movement.
-
-	Structural mechanism of the dynein power stroke.,Lin J, Okada K, Raytchev M, Smith MC, Nicastro D Nat Cell Biol. 2014 Apr 13. doi: 10.1038/ncb2939. PMID:24727830<ref>PMID:24727830</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3j67" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 14:16, 24 January 2024

OCA — Wed, 24 Jan 2024 14:16:07 GMT

←Older revision		Revision as of 14:16, 24 January 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3j67]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Strongylocentrotus_purpuratus Strongylocentrotus purpuratus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J67 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J67 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3j67]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Strongylocentrotus_purpuratus Strongylocentrotus purpuratus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J67 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J67 FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'~~>~~[[4aki\|4aki]]~~, [[~~3j68~~\|~~3j68~~]]~~</div>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 34Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j67 OCA], [https://pdbe.org/3j67 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j67 RCSB], [https://www.ebi.ac.uk/pdbsum/3j67 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j67 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j67 OCA], [https://pdbe.org/3j67 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j67 RCSB], [https://www.ebi.ac.uk/pdbsum/3j67 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j67 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/DYHC_YEAST DYHC_YEAST] Cytoplasmic dynein acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. Required to maintain uniform nuclear distribution in hyphae. May play an important role in the proper orientation of the mitotic spindle into the budding daughter cell yeast. Probably required for normal progression of the cell cycle.<ref>PMID:15642746</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 27:
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Strongylocentrotus purpuratus]]		[[Category: Strongylocentrotus purpuratus]]
-	[[Category: Lin, J]]	+	[[Category: Lin J]]
-	[[Category: Nicastro, D]]	+	[[Category: Nicastro D]]
-	[[Category: Okada, K]]	+	[[Category: Okada K]]
-	[[Category: Raytchev, M]]	+	[[Category: Raytchev M]]
-	[[Category: Smith~~, M C]]~~	+	[[Category: Smith MC]]
-	~~[[Category: Motor protein~~]]	+

OCA at 03:50, 21 April 2022

OCA — Thu, 21 Apr 2022 03:50:26 GMT

←Older revision		Revision as of 03:50, 21 April 2022
Line 3:		Line 3:
	<SX load='3j67' size='340' side='right' viewer='molstar' caption='[[3j67]], [[Resolution\|resolution]] 34.00Å' scene=''>		<SX load='3j67' size='340' side='right' viewer='molstar' caption='[[3j67]], [[Resolution\|resolution]] 34.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3j67]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Strongylocentrotus_purpuratus Strongylocentrotus purpuratus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J67 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=3J67 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3j67]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Strongylocentrotus_purpuratus Strongylocentrotus purpuratus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J67 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J67 FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4aki\|4aki]], [[3j68\|3j68]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[4aki\|4aki]], [[3j68\|3j68]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=3j67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j67 OCA], [~~http~~://pdbe.org/3j67 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3j67 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3j67 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3j67 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j67 OCA], [https://pdbe.org/3j67 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j67 RCSB], [https://www.ebi.ac.uk/pdbsum/3j67 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j67 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 17:58, 10 April 2020

OCA — Fri, 10 Apr 2020 17:58:55 GMT

←Older revision		Revision as of 17:58, 10 April 2020
Line 3:		Line 3:
	<SX load='3j67' size='340' side='right' viewer='molstar' caption='[[3j67]], [[Resolution\|resolution]] 34.00Å' scene=''>		<SX load='3j67' size='340' side='right' viewer='molstar' caption='[[3j67]], [[Resolution\|resolution]] 34.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3j67]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Strongylocentrotus_purpuratus Strongylocentrotus purpuratus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J67 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3J67 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3j67]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Strongylocentrotus_purpuratus Strongylocentrotus purpuratus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J67 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3J67 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4aki\|4aki]], [[3j68\|3j68]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4aki\|4aki]], [[3j68\|3j68]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3j67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j67 OCA], [http://pdbe.org/3j67 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3j67 RCSB], [http://www.ebi.ac.uk/pdbsum/3j67 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3j67 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3j67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j67 OCA], [http://pdbe.org/3j67 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3j67 RCSB], [http://www.ebi.ac.uk/pdbsum/3j67 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3j67 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 17:25, 6 March 2020

OCA — Fri, 06 Mar 2020 17:25:10 GMT

←Older revision		Revision as of 17:25, 6 March 2020
Line 1:		Line 1:

	==Structural mechanism of the dynein powerstroke (post-powerstroke state)==		==Structural mechanism of the dynein powerstroke (post-powerstroke state)==
-	<~~StructureSection~~ load='3j67' size='340' side='right' caption='[[3j67]], [[Resolution\|resolution]] 34.00Å' scene=''>	+	<SX load='3j67' size='340' side='right' viewer='molstar' caption='[[3j67]], [[Resolution\|resolution]] 34.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3j67]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Strongylocentrotus_purpuratus Strongylocentrotus purpuratus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J67 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J67 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3j67]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Strongylocentrotus_purpuratus Strongylocentrotus purpuratus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J67 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J67 FirstGlance]. <br>
Line 18:		Line 18:

	==See Also==		==See Also==
-	*[[Dynein\|Dynein]]	+	*[[Dynein 3D structures\|Dynein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
		+	[[Category: Large Structures]]
	[[Category: Strongylocentrotus purpuratus]]		[[Category: Strongylocentrotus purpuratus]]
	[[Category: Lin, J]]		[[Category: Lin, J]]

OCA at 09:22, 5 August 2016

OCA — Fri, 05 Aug 2016 09:22:26 GMT

←Older revision		Revision as of 09:22, 5 August 2016
Line 1:		Line 1:
		+
	==Structural mechanism of the dynein powerstroke (post-powerstroke state)==		==Structural mechanism of the dynein powerstroke (post-powerstroke state)==
	<StructureSection load='3j67' size='340' side='right' caption='[[3j67]], [[Resolution\|resolution]] 34.00Å' scene=''>		<StructureSection load='3j67' size='340' side='right' caption='[[3j67]], [[Resolution\|resolution]] 34.00Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[3j67]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Strongylocentrotus_purpuratus Strongylocentrotus purpuratus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J67 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J67 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3j67]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Strongylocentrotus_purpuratus Strongylocentrotus purpuratus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J67 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J67 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4aki\|4aki]], [[3j68\|3j68]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4aki\|4aki]], [[3j68\|3j68]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j67 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3j67 RCSB], [http://www.ebi.ac.uk/pdbsum/3j67 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j67 OCA], [http://pdbe.org/3j67 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3j67 RCSB], [http://www.ebi.ac.uk/pdbsum/3j67 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3j67 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 14:		Line 15:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3j67" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Dynein\|Dynein]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 22:38, 3 January 2015

OCA — Sat, 03 Jan 2015 22:38:30 GMT

←Older revision		Revision as of 22:38, 3 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3j67]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Strongylocentrotus_purpuratus Strongylocentrotus purpuratus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J67 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J67 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3j67]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Strongylocentrotus_purpuratus Strongylocentrotus purpuratus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J67 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J67 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4aki\|4aki]], [[3j68\|3j68]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4aki\|4aki]], [[3j68\|3j68]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j67 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3j67 RCSB], [http://www.ebi.ac.uk/pdbsum/3j67 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j67 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3j67 RCSB], [http://www.ebi.ac.uk/pdbsum/3j67 PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 12:		Line 12:
	Structural mechanism of the dynein power stroke.,Lin J, Okada K, Raytchev M, Smith MC, Nicastro D Nat Cell Biol. 2014 Apr 13. doi: 10.1038/ncb2939. PMID:24727830<ref>PMID:24727830</ref>		Structural mechanism of the dynein power stroke.,Lin J, Okada K, Raytchev M, Smith MC, Nicastro D Nat Cell Biol. 2014 Apr 13. doi: 10.1038/ncb2939. PMID:24727830<ref>PMID:24727830</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 19:		Line 19:
	</StructureSection>		</StructureSection>
	[[Category: Strongylocentrotus purpuratus]]		[[Category: Strongylocentrotus purpuratus]]
-	[[Category: Lin, J.]]	+	[[Category: Lin, J]]
-	[[Category: Nicastro, D.]]	+	[[Category: Nicastro, D]]
-	[[Category: Okada, K.]]	+	[[Category: Okada, K]]
-	[[Category: Raytchev, M.]]	+	[[Category: Raytchev, M]]
-	[[Category: Smith, M C.]]	+	[[Category: Smith, M C]]
	[[Category: Motor protein]]		[[Category: Motor protein]]

OCA at 06:55, 21 May 2014

OCA — Wed, 21 May 2014 06:55:55 GMT

←Older revision		Revision as of 06:55, 21 May 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3j67\| PDB=3j67 \| SCENE= }}~~	+	==Structural mechanism of the dynein powerstroke (post-powerstroke state)==
-	===Structural mechanism of the dynein powerstroke (post-powerstroke state)===	+	<StructureSection load='3j67' size='340' side='right' caption='[[3j67]], [[Resolution\|resolution]] 34.00Å' scene=''>
-	~~{{ABSTRACT_PUBMED_24727830}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3j67]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Strongylocentrotus_purpuratus Strongylocentrotus purpuratus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J67 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J67 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4aki\|4aki]], [[3j68\|3j68]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j67 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3j67 RCSB], [http://www.ebi.ac.uk/pdbsum/3j67 PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Dyneins are large microtubule motor proteins required for mitosis, intracellular transport and ciliary and flagellar motility. They generate force through a power-stroke mechanism, which is an ATP-consuming cycle of pre- and post-power-stroke conformational changes that cause relative motion between different dynein domains. However, key structural details of dynein's force generation remain elusive. Here, using cryo-electron tomography of intact, active (that is, beating), rapidly frozen sea urchin sperm flagella, we determined the in situ three-dimensional structures of all domains of both pre- and post-power-stroke dynein, including the previously unresolved linker and stalk of pre-power-stroke dynein. Our results reveal that the rotation of the head relative to the linker is the key action in dynein movement, and that there are at least two distinct pre-power-stroke conformations: pre-I (microtubule-detached) and pre-II (microtubule-bound). We provide three-dimensional reconstructions of native dyneins in three conformational states, in situ, allowing us to propose a molecular model of the structural cycle underlying dynein movement.

-	~~==About this Structure==~~	+	Structural mechanism of the dynein power stroke.,Lin J, Okada K, Raytchev M, Smith MC, Nicastro D Nat Cell Biol. 2014 Apr 13. doi: 10.1038/ncb2939. PMID:24727830<ref>PMID:24727830</ref>
-	~~[[3j67]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Strongylocentrotus_purpuratus Strongylocentrotus purpuratus]~~. ~~Full crystallographic information is available from [http~~:/~~/oca~~.~~weizmann.ac.il~~/~~oca-bin/ocashort?id=3J67 OCA].~~	+

-	==~~Reference~~==	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:024727830</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Strongylocentrotus purpuratus]]		[[Category: Strongylocentrotus purpuratus]]
	[[Category: Lin, J.]]		[[Category: Lin, J.]]

OCA at 07:37, 23 April 2014

OCA — Wed, 23 Apr 2014 07:37:03 GMT

←Older revision		Revision as of 07:37, 23 April 2014
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_3j67\| PDB=3j67 \| SCENE= }}
		+	===Structural mechanism of the dynein powerstroke (post-powerstroke state)===
		+	{{ABSTRACT_PUBMED_24727830}}

-	~~The entry~~ 3j67 is ~~ON HOLD until Paper Publication~~	+	==About this Structure==
		+	[[3j67]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Strongylocentrotus_purpuratus Strongylocentrotus purpuratus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J67 OCA].

-	~~Authors~~: Lin, J., Okada, K., Raytchev, M., Smith, M.C.~~, Nicastro, D.~~	+	==Reference==
-		+	<ref group="xtra">PMID:024727830</ref><references group="xtra"/><references/>
-	~~Description~~: ~~Structural mechanism of the dynein powerstroke (post-powerstroke state)~~	+	[[Category: Strongylocentrotus purpuratus]]
		+	[[Category: Lin, J.]]
		+	[[Category: Nicastro, D.]]
		+	[[Category: Okada, K.]]
		+	[[Category: Raytchev, M.]]
		+	[[Category: Smith, M C.]]
		+	[[Category: Motor protein]]

OCA at 09:39, 5 February 2014

OCA — Wed, 05 Feb 2014 09:39:12 GMT

←Older revision		Revision as of 09:39, 5 February 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3j67 is ON HOLD	+	The entry 3j67 is ON HOLD until Paper Publication

	Authors: Lin, J., Okada, K., Raytchev, M., Smith, M.C., Nicastro, D.		Authors: Lin, J., Okada, K., Raytchev, M., Smith, M.C., Nicastro, D.

-	Description: Structural mechanism of the dynein powerstroke ~~inside cells~~ (post-powerstroke state)	+	Description: Structural mechanism of the dynein powerstroke (post-powerstroke state)