3j6u - Revision history

OCA at 10:10, 21 February 2024

OCA — Wed, 21 Feb 2024 10:10:46 GMT

←Older revision		Revision as of 10:10, 21 February 2024
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	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/A9LID6_9FLAV A9LID6_9FLAV]		[https://www.uniprot.org/uniprot/A9LID6_9FLAV A9LID6_9FLAV]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Dengue virus (DENV) infects ~400 million people annually. There is no licensed vaccine or therapeutic drug. Only a small fraction of the total DENV-specific antibodies in a naturally occurring dengue infection consists of highly neutralizing antibodies. Here we show that the DENV-specific human monoclonal antibody 5J7 is exceptionally potent, neutralizing 50% of virus at nanogram-range antibody concentration. The 9 A resolution cryo-electron microscopy structure of the Fab 5J7-DENV complex shows that a single Fab molecule binds across three envelope proteins and engages three functionally important domains, each from a different envelope protein. These domains are critical for receptor binding and fusion to the endosomal membrane. The ability to bind to multiple domains allows the antibody to fully coat the virus surface with only 60 copies of Fab, that is, half the amount compared with other potent antibodies. Our study reveals a highly efficient and unusual mechanism of molecular recognition by an antibody.
-
-	A highly potent human antibody neutralizes dengue virus serotype 3 by binding across three surface proteins.,Fibriansah G, Tan JL, Smith SA, de Alwis R, Ng TS, Kostyuchenko VA, Jadi RS, Kukkaro P, de Silva AM, Crowe JE, Lok SM Nat Commun. 2015 Feb 20;6:6341. doi: 10.1038/ncomms7341. PMID:25698059<ref>PMID:25698059</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3j6u" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</SX>		</SX>

OCA at 14:16, 24 January 2024

OCA — Wed, 24 Jan 2024 14:16:48 GMT

←Older revision		Revision as of 14:16, 24 January 2024
Line 3:		Line 3:
	<SX load='3j6u' size='340' side='right' viewer='molstar' caption='[[3j6u]], [[Resolution\|resolution]] 9.00Å' scene=''>		<SX load='3j6u' size='340' side='right' viewer='molstar' caption='[[3j6u]], [[Resolution\|resolution]] 9.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3j6u]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Dengue_virus_3 Dengue virus 3] and [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J6U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J6U FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3j6u]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Dengue_virus_3 Dengue virus 3] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J6U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J6U FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'~~>~~[[3j6s\|3j6s]]~~, [[~~3j6t~~\|~~3j6t~~]]~~</div>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 9Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j6u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j6u OCA], [https://pdbe.org/3j6u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j6u RCSB], [https://www.ebi.ac.uk/pdbsum/3j6u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j6u ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j6u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j6u OCA], [https://pdbe.org/3j6u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j6u RCSB], [https://www.ebi.ac.uk/pdbsum/3j6u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j6u ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/Q6DLV0_9FLAV Q6DLV0_9FLAV]] Envelope protein E binding to host cell surface receptor is followed by virus internalization through clathrin-mediated endocytosis. Envelope protein E is subsequently involved in membrane fusion between virion and host late endosomes. Synthesized as a homodimer with prM which acts as a chaperone for envelope protein E. After cleavage of prM, envelope protein E dissociate from small envelope protein M and homodimerizes (By similarity).[SAAS:SAAS000336_004_099774]	+	[https://www.uniprot.org/uniprot/A9LID6_9FLAV A9LID6_9FLAV]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</SX>		</SX>
	[[Category: Dengue virus 3]]		[[Category: Dengue virus 3]]
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Alwis, R de]]~~	+	[[Category: Crowe Jr JE]]
-	[[Category: Crowe~~, J E~~]]	+	[[Category: Fibriansah G]]
-	[[Category: Fibriansah, G]]	+	[[Category: Kostyuchenko VA]]
-	[[Category: Kostyuchenko~~, V A~~]]	+	[[Category: Kukkaro P]]
-	[[Category: Kukkaro, P]]	+	[[Category: Lok S-M]]
-	[[Category: Lok, S M]]	+	[[Category: Ng T-S]]
-	[[Category: Ng, T S]]	+	[[Category: Smith SA]]
-	~~[[Category: Silva, A M.de~~]]	+	[[Category: Tan JL]]
-	[[Category: Smith~~, S A~~]]	+	[[Category: De Alwis R]]
-	[[Category: Tan~~, J L~~]]	+	[[Category: De Silva AM]]
-	[[Category: ~~Dengue virus~~]]	+
-	[[Category: ~~Human antibody]]~~	+
-	~~[[Category: Neutralization]]~~	+
-	~~[[Category: Virus-immune system complex~~]]	+

OCA at 03:51, 21 April 2022

OCA — Thu, 21 Apr 2022 03:51:01 GMT

←Older revision		Revision as of 03:51, 21 April 2022
Line 3:		Line 3:
	<SX load='3j6u' size='340' side='right' viewer='molstar' caption='[[3j6u]], [[Resolution\|resolution]] 9.00Å' scene=''>		<SX load='3j6u' size='340' side='right' viewer='molstar' caption='[[3j6u]], [[Resolution\|resolution]] 9.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3j6u]] is a 8 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Dengue_virus_3 Dengue virus 3] and [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J6U OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=3J6U FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3j6u]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Dengue_virus_3 Dengue virus 3] and [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J6U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J6U FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3j6s\|3j6s]], [[3j6t\|3j6t]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3j6s\|3j6s]], [[3j6t\|3j6t]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=3j6u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j6u OCA], [~~http~~://pdbe.org/3j6u PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3j6u RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3j6u PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3j6u ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j6u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j6u OCA], [https://pdbe.org/3j6u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j6u RCSB], [https://www.ebi.ac.uk/pdbsum/3j6u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j6u ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/Q6DLV0_9FLAV Q6DLV0_9FLAV]] Envelope protein E binding to host cell surface receptor is followed by virus internalization through clathrin-mediated endocytosis. Envelope protein E is subsequently involved in membrane fusion between virion and host late endosomes. Synthesized as a homodimer with prM which acts as a chaperone for envelope protein E. After cleavage of prM, envelope protein E dissociate from small envelope protein M and homodimerizes (By similarity).[SAAS:SAAS000336_004_099774]	+	[[https://www.uniprot.org/uniprot/Q6DLV0_9FLAV Q6DLV0_9FLAV]] Envelope protein E binding to host cell surface receptor is followed by virus internalization through clathrin-mediated endocytosis. Envelope protein E is subsequently involved in membrane fusion between virion and host late endosomes. Synthesized as a homodimer with prM which acts as a chaperone for envelope protein E. After cleavage of prM, envelope protein E dissociate from small envelope protein M and homodimerizes (By similarity).[SAAS:SAAS000336_004_099774]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 18:02, 10 April 2020

OCA — Fri, 10 Apr 2020 18:02:52 GMT

←Older revision		Revision as of 18:02, 10 April 2020
Line 3:		Line 3:
	<SX load='3j6u' size='340' side='right' viewer='molstar' caption='[[3j6u]], [[Resolution\|resolution]] 9.00Å' scene=''>		<SX load='3j6u' size='340' side='right' viewer='molstar' caption='[[3j6u]], [[Resolution\|resolution]] 9.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3j6u]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Dengue_virus_3 Dengue virus 3] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J6U OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3J6U FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3j6u]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Dengue_virus_3 Dengue virus 3] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J6U OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3J6U FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3j6s\|3j6s]], [[3j6t\|3j6t]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3j6s\|3j6s]], [[3j6t\|3j6t]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3j6u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j6u OCA], [http://pdbe.org/3j6u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3j6u RCSB], [http://www.ebi.ac.uk/pdbsum/3j6u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3j6u ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3j6u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j6u OCA], [http://pdbe.org/3j6u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3j6u RCSB], [http://www.ebi.ac.uk/pdbsum/3j6u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3j6u ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 04:28, 5 March 2020

OCA — Thu, 05 Mar 2020 04:28:01 GMT

←Older revision		Revision as of 04:28, 5 March 2020
Line 1:		Line 1:

	==Cryo-EM structure of Dengue virus serotype 3 in complex with human antibody 5J7 Fab==		==Cryo-EM structure of Dengue virus serotype 3 in complex with human antibody 5J7 Fab==
-	<~~StructureSection~~ load='3j6u' size='340' side='right' caption='[[3j6u]], [[Resolution\|resolution]] 9.00Å' scene=''>	+	<SX load='3j6u' size='340' side='right' viewer='molstar' caption='[[3j6u]], [[Resolution\|resolution]] 9.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3j6u]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Dengue_virus_3 Dengue virus 3] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J6U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J6U FirstGlance]. <br>		<table><tr><td colspan='2'>[[3j6u]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Dengue_virus_3 Dengue virus 3] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J6U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J6U FirstGlance]. <br>
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	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Dengue virus 3]]		[[Category: Dengue virus 3]]
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Alwis, R de]]		[[Category: Alwis, R de]]
	[[Category: Crowe, J E]]		[[Category: Crowe, J E]]

OCA at 08:14, 18 July 2018

OCA — Wed, 18 Jul 2018 08:14:20 GMT

←Older revision		Revision as of 08:14, 18 July 2018
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	<StructureSection load='3j6u' size='340' side='right' caption='[[3j6u]], [[Resolution\|resolution]] 9.00Å' scene=''>		<StructureSection load='3j6u' size='340' side='right' caption='[[3j6u]], [[Resolution\|resolution]] 9.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3j6u]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J6U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J6U FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3j6u]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Dengue_virus_3 Dengue virus 3] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J6U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J6U FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3j6s\|3j6s]], [[3j6t\|3j6t]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3j6s\|3j6s]], [[3j6t\|3j6t]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j6u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j6u OCA], [http://pdbe.org/3j6u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3j6u RCSB], [http://www.ebi.ac.uk/pdbsum/3j6u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3j6u ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j6u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j6u OCA], [http://pdbe.org/3j6u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3j6u RCSB], [http://www.ebi.ac.uk/pdbsum/3j6u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3j6u ProSAT]</span></td></tr>
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Dengue virus 3]]
		+	[[Category: Human]]
	[[Category: Alwis, R de]]		[[Category: Alwis, R de]]
	[[Category: Crowe, J E]]		[[Category: Crowe, J E]]

OCA at 20:22, 9 December 2016

OCA — Fri, 09 Dec 2016 20:22:27 GMT

←Older revision		Revision as of 20:22, 9 December 2016
Line 1:		Line 1:
		+
	==Cryo-EM structure of Dengue virus serotype 3 in complex with human antibody 5J7 Fab==		==Cryo-EM structure of Dengue virus serotype 3 in complex with human antibody 5J7 Fab==
	<StructureSection load='3j6u' size='340' side='right' caption='[[3j6u]], [[Resolution\|resolution]] 9.00Å' scene=''>		<StructureSection load='3j6u' size='340' side='right' caption='[[3j6u]], [[Resolution\|resolution]] 9.00Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[3j6u]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J6U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J6U FirstGlance]. <br>		<table><tr><td colspan='2'>[[3j6u]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J6U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J6U FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3j6s\|3j6s]], [[3j6t\|3j6t]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3j6s\|3j6s]], [[3j6t\|3j6t]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j6u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j6u OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3j6u RCSB], [http://www.ebi.ac.uk/pdbsum/3j6u PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j6u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j6u OCA], [http://pdbe.org/3j6u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3j6u RCSB], [http://www.ebi.ac.uk/pdbsum/3j6u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3j6u ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3j6u" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:24, 4 March 2015

OCA — Wed, 04 Mar 2015 11:24:37 GMT

←Older revision		Revision as of 11:24, 4 March 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Cryo-EM structure of Dengue virus serotype 3 in complex with human antibody 5J7 Fab==
		+	<StructureSection load='3j6u' size='340' side='right' caption='[[3j6u]], [[Resolution\|resolution]] 9.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3j6u]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J6U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J6U FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3j6s\|3j6s]], [[3j6t\|3j6t]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j6u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j6u OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3j6u RCSB], [http://www.ebi.ac.uk/pdbsum/3j6u PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/Q6DLV0_9FLAV Q6DLV0_9FLAV]] Envelope protein E binding to host cell surface receptor is followed by virus internalization through clathrin-mediated endocytosis. Envelope protein E is subsequently involved in membrane fusion between virion and host late endosomes. Synthesized as a homodimer with prM which acts as a chaperone for envelope protein E. After cleavage of prM, envelope protein E dissociate from small envelope protein M and homodimerizes (By similarity).[SAAS:SAAS000336_004_099774]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Dengue virus (DENV) infects ~400 million people annually. There is no licensed vaccine or therapeutic drug. Only a small fraction of the total DENV-specific antibodies in a naturally occurring dengue infection consists of highly neutralizing antibodies. Here we show that the DENV-specific human monoclonal antibody 5J7 is exceptionally potent, neutralizing 50% of virus at nanogram-range antibody concentration. The 9 A resolution cryo-electron microscopy structure of the Fab 5J7-DENV complex shows that a single Fab molecule binds across three envelope proteins and engages three functionally important domains, each from a different envelope protein. These domains are critical for receptor binding and fusion to the endosomal membrane. The ability to bind to multiple domains allows the antibody to fully coat the virus surface with only 60 copies of Fab, that is, half the amount compared with other potent antibodies. Our study reveals a highly efficient and unusual mechanism of molecular recognition by an antibody.

-	~~The entry 3j6u is ON HOLD until Paper Publication~~	+	A highly potent human antibody neutralizes dengue virus serotype 3 by binding across three surface proteins.,Fibriansah G, Tan JL, Smith SA, de Alwis R, Ng TS, Kostyuchenko VA, Jadi RS, Kukkaro P, de Silva AM, Crowe JE, Lok SM Nat Commun. 2015 Feb 20;6:6341. doi: 10.1038/ncomms7341. PMID:25698059<ref>PMID:25698059</ref>

-	~~Authors: Fibriansah~~, G.~~, Tan, J.L., Smith,~~ S.~~A., de Alwis, R., Ng, T.-S., Kostyuchenko, V.A., Kukkaro, P., de Silva, A.M., Crowe Jr., J.E., Lok, S.-M~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description: Cryo-EM structure of Dengue virus serotype 3 in complex with human antibody 5J7 Fab~~	+	== References ==
-	~~[[Category: Unreleased Structures]]~~	+	<references/>
-	[[Category: De Alwis, R]]	+	__TOC__
-	[[Category: ~~Tan~~, J.L]]	+	</StructureSection>
-	[[Category: ~~Lok~~, ~~S.-M~~]]	+	[[Category: Alwis, R de]]
-	[[Category: Kostyuchenko, V.A]]	+	[[Category: Crowe, J E]]
-	~~[[Category: Crowe Jr]]~~	+	[[Category: Fibriansah, G]]
-	~~[[Category: J.E]]~~	+	[[Category: Kostyuchenko, V A]]
-	~~[[Category: Ng, T.-S]]~~	+
-	~~[[Category: De Silva, A.M~~]]	+
	[[Category: Kukkaro, P]]		[[Category: Kukkaro, P]]
-	[[Category: ~~Smith~~, S.A]]	+	[[Category: Lok, S M]]
-	[[Category: ~~Fibriansah~~, G]]	+	[[Category: Ng, T S]]
		+	[[Category: Silva, A M.de]]
		+	[[Category: Smith, S A]]
		+	[[Category: Tan, J L]]
		+	[[Category: Dengue virus]]
		+	[[Category: Human antibody]]
		+	[[Category: Neutralization]]
		+	[[Category: Virus-immune system complex]]

OCA at 12:39, 24 December 2014

OCA — Wed, 24 Dec 2014 12:39:16 GMT

←Older revision		Revision as of 12:39, 24 December 2014
Line 6:		Line 6:

	Description: Cryo-EM structure of Dengue virus serotype 3 in complex with human antibody 5J7 Fab		Description: Cryo-EM structure of Dengue virus serotype 3 in complex with human antibody 5J7 Fab
		+	[[Category: Unreleased Structures]]
		+	[[Category: De Alwis, R]]
		+	[[Category: Tan, J.L]]
		+	[[Category: Lok, S.-M]]
		+	[[Category: Kostyuchenko, V.A]]
		+	[[Category: Crowe Jr]]
		+	[[Category: J.E]]
		+	[[Category: Ng, T.-S]]
		+	[[Category: De Silva, A.M]]
		+	[[Category: Kukkaro, P]]
		+	[[Category: Smith, S.A]]
		+	[[Category: Fibriansah, G]]

OCA at 07:30, 24 September 2014

OCA — Wed, 24 Sep 2014 07:30:41 GMT

←Older revision		Revision as of 07:30, 24 September 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3j6u is ON HOLD	+	The entry 3j6u is ON HOLD until Paper Publication

	Authors: Fibriansah, G., Tan, J.L., Smith, S.A., de Alwis, R., Ng, T.-S., Kostyuchenko, V.A., Kukkaro, P., de Silva, A.M., Crowe Jr., J.E., Lok, S.-M.		Authors: Fibriansah, G., Tan, J.L., Smith, S.A., de Alwis, R., Ng, T.-S., Kostyuchenko, V.A., Kukkaro, P., de Silva, A.M., Crowe Jr., J.E., Lok, S.-M.

	Description: Cryo-EM structure of Dengue virus serotype 3 in complex with human antibody 5J7 Fab		Description: Cryo-EM structure of Dengue virus serotype 3 in complex with human antibody 5J7 Fab