3j7x - Revision history

OCA at 10:11, 21 February 2024

OCA — Wed, 21 Feb 2024 10:11:08 GMT

←Older revision		Revision as of 10:11, 21 February 2024
Line 9:		Line 9:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/CAPSA_BPT7 CAPSA_BPT7] Assembles with the minor capsid protein to form an icosahedral capsid with a T=7 symmetry, about 60 nm in diameter, and consisting of 415 capsid proteins. The major and minor capsid proteins are incorporated into the capsid in about a 90/10 ratio respectively. Once the capsid is formed, encapsidates one single copy of the viral genome.<ref>PMID:20962334</ref>		[https://www.uniprot.org/uniprot/CAPSA_BPT7 CAPSA_BPT7] Assembles with the minor capsid protein to form an icosahedral capsid with a T=7 symmetry, about 60 nm in diameter, and consisting of 415 capsid proteins. The major and minor capsid proteins are incorporated into the capsid in about a 90/10 ratio respectively. Once the capsid is formed, encapsidates one single copy of the viral genome.<ref>PMID:20962334</ref>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Many dsDNA viruses first assemble a DNA-free procapsid, using a scaffolding protein-dependent process. The procapsid, then, undergoes dramatic conformational maturation while packaging DNA. For bacteriophage T7 we report the following four single-particle cryo-EM 3D reconstructions and the derived atomic models: procapsid (4.6-A resolution), an early-stage DNA packaging intermediate (3.5 A), a later-stage packaging intermediate (6.6 A), and the final infectious phage (3.6 A). In the procapsid, the N terminus of the major capsid protein, gp10, has a six-turn helix at the inner surface of the shell, where each skewed hexamer of gp10 interacts with two scaffolding proteins. With the exit of scaffolding proteins during maturation the gp10 N-terminal helix unfolds and swings through the capsid shell to the outer surface. The refolded N-terminal region has a hairpin that forms a novel noncovalent, joint-like, intercapsomeric interaction with a pocket formed during shell expansion. These large conformational changes also result in a new noncovalent, intracapsomeric topological linking. Both interactions further stabilize the capsids by interlocking all pentameric and hexameric capsomeres in both DNA packaging intermediate and phage. Although the final phage shell has nearly identical structure to the shell of the DNA-free intermediate, surprisingly we found that the icosahedral faces of the phage are slightly ( approximately 4 A) contracted relative to the faces of the intermediate, despite the internal pressure from the densely packaged DNA genome. These structures provide a basis for understanding the capsid maturation process during DNA packaging that is essential for large numbers of dsDNA viruses.
-
-	Capsid expansion mechanism of bacteriophage T7 revealed by multistate atomic models derived from cryo-EM reconstructions.,Guo F, Liu Z, Fang PA, Zhang Q, Wright ET, Wu W, Zhang C, Vago F, Ren Y, Jakana J, Chiu W, Serwer P, Jiang W Proc Natl Acad Sci U S A. 2014 Oct 13. pii: 201407020. PMID:25313071<ref>PMID:25313071</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3j7x" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 14:17, 24 January 2024

OCA — Wed, 24 Jan 2024 14:17:33 GMT

←Older revision		Revision as of 14:17, 24 January 2024
Line 3:		Line 3:
	<SX load='3j7x' size='340' side='right' viewer='molstar' caption='[[3j7x]], [[Resolution\|resolution]] 3.80Å' scene=''>		<SX load='3j7x' size='340' side='right' viewer='molstar' caption='[[3j7x]], [[Resolution\|resolution]] 3.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3j7x]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Enterobacteria_phage_t7 Enterobacteria~~ phage t7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J7X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J7X FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3j7x]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_phage_T7 Escherichia phage T7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J7X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J7X FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'~~>~~[[3j7v\|3j7v]]~~, [[~~3j7w~~\|~~3j7w~~]]~~</div>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.8Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j7x OCA], [https://pdbe.org/3j7x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j7x RCSB], [https://www.ebi.ac.uk/pdbsum/3j7x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j7x ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j7x OCA], [https://pdbe.org/3j7x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j7x RCSB], [https://www.ebi.ac.uk/pdbsum/3j7x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j7x ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/CAPSA_BPT7 CAPSA_BPT7] Assembles with the minor capsid protein to form an icosahedral capsid with a T=7 symmetry, about 60 nm in diameter, and consisting of 415 capsid proteins. The major and minor capsid proteins are incorporated into the capsid in about a 90/10 ratio respectively. Once the capsid is formed, encapsidates one single copy of the viral genome.<ref>PMID:20962334</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</SX>		</SX>
-	[[Category: ~~Enterobacteria~~ phage t7]]	+	[[Category: Escherichia phage T7]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Chiu, W]]	+	[[Category: Chiu W]]
-	[[Category: Fang~~, P A~~]]	+	[[Category: Fang PA]]
-	[[Category: Guo, F]]	+	[[Category: Guo F]]
-	[[Category: Jakata, J]]	+	[[Category: Jakata J]]
-	[[Category: Jiang, W]]	+	[[Category: Jiang W]]
-	[[Category: Liu, Z]]	+	[[Category: Liu Z]]
-	[[Category: Ren, Y]]	+	[[Category: Ren Y]]
-	[[Category: Serwer, P]]	+	[[Category: Serwer P]]
-	[[Category: Vago, F]]	+	[[Category: Vago F]]
-	[[Category: Wright~~, E T~~]]	+	[[Category: Wright ET]]
-	[[Category: Wu, W]]	+	[[Category: Wu W]]
-	[[Category: Zhang, C]]	+	[[Category: Zhang C]]
-	[[Category: Zhang, Q]]	+	[[Category: Zhang Q]]
-	~~[[Category: Dna packaging]]~~	+
-	~~[[Category: Maturation]]~~	+
-	~~[[Category: Non-covalent topological linking]]~~	+
-	~~[[Category: Procapsid]]~~	+
-	~~[[Category: Virus~~]]	+

OCA at 03:51, 21 April 2022

OCA — Thu, 21 Apr 2022 03:51:39 GMT

←Older revision		Revision as of 03:51, 21 April 2022
Line 3:		Line 3:
	<SX load='3j7x' size='340' side='right' viewer='molstar' caption='[[3j7x]], [[Resolution\|resolution]] 3.80Å' scene=''>		<SX load='3j7x' size='340' side='right' viewer='molstar' caption='[[3j7x]], [[Resolution\|resolution]] 3.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3j7x]] is a 7 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Enterobacteria_phage_t7 Enterobacteria phage t7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J7X OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=3J7X FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3j7x]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterobacteria_phage_t7 Enterobacteria phage t7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J7X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J7X FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3j7v\|3j7v]], [[3j7w\|3j7w]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3j7v\|3j7v]], [[3j7w\|3j7w]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=3j7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j7x OCA], [~~http~~://pdbe.org/3j7x PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3j7x RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3j7x PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3j7x ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j7x OCA], [https://pdbe.org/3j7x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j7x RCSB], [https://www.ebi.ac.uk/pdbsum/3j7x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j7x ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 18:06, 10 April 2020

OCA — Fri, 10 Apr 2020 18:06:59 GMT

←Older revision		Revision as of 18:06, 10 April 2020
Line 3:		Line 3:
	<SX load='3j7x' size='340' side='right' viewer='molstar' caption='[[3j7x]], [[Resolution\|resolution]] 3.80Å' scene=''>		<SX load='3j7x' size='340' side='right' viewer='molstar' caption='[[3j7x]], [[Resolution\|resolution]] 3.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3j7x]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t7 Enterobacteria phage t7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J7X OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3J7X FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3j7x]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t7 Enterobacteria phage t7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J7X OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3J7X FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3j7v\|3j7v]], [[3j7w\|3j7w]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3j7v\|3j7v]], [[3j7w\|3j7w]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3j7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j7x OCA], [http://pdbe.org/3j7x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3j7x RCSB], [http://www.ebi.ac.uk/pdbsum/3j7x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3j7x ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3j7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j7x OCA], [http://pdbe.org/3j7x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3j7x RCSB], [http://www.ebi.ac.uk/pdbsum/3j7x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3j7x ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 04:28, 5 March 2020

OCA — Thu, 05 Mar 2020 04:28:22 GMT

←Older revision		Revision as of 04:28, 5 March 2020
Line 1:		Line 1:

	==Capsid Expansion Mechanism Of Bacteriophage T7 Revealed By Multi-State Atomic Models Derived From Cryo-EM Reconstructions==		==Capsid Expansion Mechanism Of Bacteriophage T7 Revealed By Multi-State Atomic Models Derived From Cryo-EM Reconstructions==
-	<~~StructureSection~~ load='3j7x' size='340' side='right' caption='[[3j7x]], [[Resolution\|resolution]] 3.80Å' scene=''>	+	<SX load='3j7x' size='340' side='right' viewer='molstar' caption='[[3j7x]], [[Resolution\|resolution]] 3.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3j7x]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t7 Enterobacteria phage t7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J7X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J7X FirstGlance]. <br>		<table><tr><td colspan='2'>[[3j7x]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t7 Enterobacteria phage t7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J7X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J7X FirstGlance]. <br>
Line 19:		Line 19:
	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Enterobacteria phage t7]]		[[Category: Enterobacteria phage t7]]
		+	[[Category: Large Structures]]
	[[Category: Chiu, W]]		[[Category: Chiu, W]]
	[[Category: Fang, P A]]		[[Category: Fang, P A]]

OCA at 09:02, 11 August 2016

OCA — Thu, 11 Aug 2016 09:02:31 GMT

←Older revision		Revision as of 09:02, 11 August 2016
Line 1:		Line 1:
		+
	==Capsid Expansion Mechanism Of Bacteriophage T7 Revealed By Multi-State Atomic Models Derived From Cryo-EM Reconstructions==		==Capsid Expansion Mechanism Of Bacteriophage T7 Revealed By Multi-State Atomic Models Derived From Cryo-EM Reconstructions==
	<StructureSection load='3j7x' size='340' side='right' caption='[[3j7x]], [[Resolution\|resolution]] 3.80Å' scene=''>		<StructureSection load='3j7x' size='340' side='right' caption='[[3j7x]], [[Resolution\|resolution]] 3.80Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[3j7x]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t7 Enterobacteria phage t7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J7X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J7X FirstGlance]. <br>		<table><tr><td colspan='2'>[[3j7x]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t7 Enterobacteria phage t7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J7X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J7X FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3j7v\|3j7v]], [[3j7w\|3j7w]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3j7v\|3j7v]], [[3j7w\|3j7w]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j7x OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3j7x RCSB], [http://www.ebi.ac.uk/pdbsum/3j7x PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j7x OCA], [http://pdbe.org/3j7x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3j7x RCSB], [http://www.ebi.ac.uk/pdbsum/3j7x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3j7x ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 14:		Line 15:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3j7x" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:25, 12 November 2014

OCA — Wed, 12 Nov 2014 07:25:25 GMT

←Older revision		Revision as of 07:25, 12 November 2014
Line 19:		Line 19:
	</StructureSection>		</StructureSection>
	[[Category: Enterobacteria phage t7]]		[[Category: Enterobacteria phage t7]]
-	[[Category: Chiu, W.]]	+	[[Category: Chiu, W]]
-	[[Category: Fang, P A.]]	+	[[Category: Fang, P A]]
-	[[Category: Guo, F.]]	+	[[Category: Guo, F]]
-	[[Category: Jakata, J.]]	+	[[Category: Jakata, J]]
-	[[Category: Jiang, W.]]	+	[[Category: Jiang, W]]
-	[[Category: Liu, Z.]]	+	[[Category: Liu, Z]]
-	[[Category: Ren, Y.]]	+	[[Category: Ren, Y]]
-	[[Category: Serwer, P.]]	+	[[Category: Serwer, P]]
-	[[Category: Vago, F.]]	+	[[Category: Vago, F]]
-	[[Category: Wright, E T.]]	+	[[Category: Wright, E T]]
-	[[Category: Wu, W.]]	+	[[Category: Wu, W]]
-	[[Category: Zhang, C.]]	+	[[Category: Zhang, C]]
-	[[Category: Zhang, Q.]]	+	[[Category: Zhang, Q]]
	[[Category: Dna packaging]]		[[Category: Dna packaging]]
	[[Category: Maturation]]		[[Category: Maturation]]

OCA at 09:17, 29 October 2014

OCA — Wed, 29 Oct 2014 09:17:01 GMT

←Older revision		Revision as of 09:17, 29 October 2014
Line 6:		Line 6:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j7x OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3j7x RCSB], [http://www.ebi.ac.uk/pdbsum/3j7x PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j7x OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3j7x RCSB], [http://www.ebi.ac.uk/pdbsum/3j7x PDBsum]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Many dsDNA viruses first assemble a DNA-free procapsid, using a scaffolding protein-dependent process. The procapsid, then, undergoes dramatic conformational maturation while packaging DNA. For bacteriophage T7 we report the following four single-particle cryo-EM 3D reconstructions and the derived atomic models: procapsid (4.6-A resolution), an early-stage DNA packaging intermediate (3.5 A), a later-stage packaging intermediate (6.6 A), and the final infectious phage (3.6 A). In the procapsid, the N terminus of the major capsid protein, gp10, has a six-turn helix at the inner surface of the shell, where each skewed hexamer of gp10 interacts with two scaffolding proteins. With the exit of scaffolding proteins during maturation the gp10 N-terminal helix unfolds and swings through the capsid shell to the outer surface. The refolded N-terminal region has a hairpin that forms a novel noncovalent, joint-like, intercapsomeric interaction with a pocket formed during shell expansion. These large conformational changes also result in a new noncovalent, intracapsomeric topological linking. Both interactions further stabilize the capsids by interlocking all pentameric and hexameric capsomeres in both DNA packaging intermediate and phage. Although the final phage shell has nearly identical structure to the shell of the DNA-free intermediate, surprisingly we found that the icosahedral faces of the phage are slightly ( approximately 4 A) contracted relative to the faces of the intermediate, despite the internal pressure from the densely packaged DNA genome. These structures provide a basis for understanding the capsid maturation process during DNA packaging that is essential for large numbers of dsDNA viruses.
		+
		+	Capsid expansion mechanism of bacteriophage T7 revealed by multistate atomic models derived from cryo-EM reconstructions.,Guo F, Liu Z, Fang PA, Zhang Q, Wright ET, Wu W, Zhang C, Vago F, Ren Y, Jakana J, Chiu W, Serwer P, Jiang W Proc Natl Acad Sci U S A. 2014 Oct 13. pii: 201407020. PMID:25313071<ref>PMID:25313071</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 10:48, 20 October 2014

OCA — Mon, 20 Oct 2014 10:48:57 GMT

←Older revision		Revision as of 10:48, 20 October 2014
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==Capsid Expansion Mechanism Of Bacteriophage T7 Revealed By Multi-State Atomic Models Derived From Cryo-EM Reconstructions==
-		+	<StructureSection load='3j7x' size='340' side='right' caption='[[3j7x]], [[Resolution\|resolution]] 3.80Å' scene=''>
-	~~The entry~~ 3j7x is ~~ON HOLD until Paper Publication~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[3j7x]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t7 Enterobacteria phage t7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J7X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J7X FirstGlance]. <br>
-	~~Authors~~: ~~Guo~~, F., ~~Liu~~, Z., Fang, P.A., ~~Zhang, Q~~., ~~Wright, E.T~~.~~, Wu~~, W., ~~Zhang~~, C., Vago, F., ~~Ren, Y~~.~~, Jakata, J., Chiu~~, W., ~~Serwer, P~~., ~~Jiang, W~~.	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3j7v\|3j7v]], [[3j7w\|3j7w]]</td></tr>
-		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j7x OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3j7x RCSB], [http://www.ebi.ac.uk/pdbsum/3j7x PDBsum]</span></td></tr>
-	~~Description~~: ~~Capsid Expansion Mechanism Of Bacteriophage T7 Revealed By Multi~~-~~State Atomic Models Derived From Cryo-EM Reconstructions~~	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Enterobacteria phage t7]]
		+	[[Category: Chiu, W.]]
		+	[[Category: Fang, P A.]]
		+	[[Category: Guo, F.]]
		+	[[Category: Jakata, J.]]
		+	[[Category: Jiang, W.]]
		+	[[Category: Liu, Z.]]
		+	[[Category: Ren, Y.]]
		+	[[Category: Serwer, P.]]
		+	[[Category: Vago, F.]]
		+	[[Category: Wright, E T.]]
		+	[[Category: Wu, W.]]
		+	[[Category: Zhang, C.]]
		+	[[Category: Zhang, Q.]]
		+	[[Category: Dna packaging]]
		+	[[Category: Maturation]]
		+	[[Category: Non-covalent topological linking]]
		+	[[Category: Procapsid]]
		+	[[Category: Virus]]

OCA at 07:31, 24 September 2014

OCA — Wed, 24 Sep 2014 07:31:18 GMT

←Older revision		Revision as of 07:31, 24 September 2014
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3j7x is ON HOLD	+	The entry 3j7x is ON HOLD until Paper Publication

	Authors: Guo, F., Liu, Z., Fang, P.A., Zhang, Q., Wright, E.T., Wu, W., Zhang, C., Vago, F., Ren, Y., Jakata, J., Chiu, W., Serwer, P., Jiang, W.		Authors: Guo, F., Liu, Z., Fang, P.A., Zhang, Q., Wright, E.T., Wu, W., Zhang, C., Vago, F., Ren, Y., Jakata, J., Chiu, W., Serwer, P., Jiang, W.

	Description: Capsid Expansion Mechanism Of Bacteriophage T7 Revealed By Multi-State Atomic Models Derived From Cryo-EM Reconstructions		Description: Capsid Expansion Mechanism Of Bacteriophage T7 Revealed By Multi-State Atomic Models Derived From Cryo-EM Reconstructions