3j80 - Revision history

OCA at 10:11, 21 February 2024

OCA — Wed, 21 Feb 2024 10:11:10 GMT

←Older revision		Revision as of 10:11, 21 February 2024
Line 10:		Line 10:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/RS21_KLULA RS21_KLULA] Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Has a physiological role leading to 18S rRNA stability (By similarity).		[https://www.uniprot.org/uniprot/RS21_KLULA RS21_KLULA] Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Has a physiological role leading to 18S rRNA stability (By similarity).
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	During eukaryotic translation initiation, initiator tRNA does not insert fully into the P decoding site on the 40S ribosomal subunit. This conformation (POUT) is compatible with scanning mRNA for the AUG start codon. Base pairing with AUG is thought to promote isomerization to a more stable conformation (PIN) that arrests scanning and promotes dissociation of eIF1 from the 40S subunit. Here, we present a cryoEM reconstruction of a yeast preinitiation complex at 4.0 A resolution with initiator tRNA in the PIN state, prior to eIF1 release. The structure reveals stabilization of the codon-anticodon duplex by the N-terminal tail of eIF1A, changes in the structure of eIF1 likely instrumental in its subsequent release, and changes in the conformation of eIF2. The mRNA traverses the entire mRNA cleft and makes connections to the regulatory domain of eIF2?, eIF1A, and ribosomal elements that allow recognition of context nucleotides surrounding the AUG codon.
-
-	Structural changes enable start codon recognition by the eukaryotic translation initiation complex.,Hussain T, Llacer JL, Fernandez IS, Munoz A, Martin-Marcos P, Savva CG, Lorsch JR, Hinnebusch AG, Ramakrishnan V Cell. 2014 Oct 23;159(3):597-607. doi: 10.1016/j.cell.2014.10.001. Epub 2014 Oct , 16. PMID:25417110<ref>PMID:25417110</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3j80" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Eukaryotic initiation factor 3D structures\|Eukaryotic initiation factor 3D structures]]		*[[Eukaryotic initiation factor 3D structures\|Eukaryotic initiation factor 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</SX>		</SX>

OCA at 14:17, 24 January 2024

OCA — Wed, 24 Jan 2024 14:17:46 GMT

←Older revision		Revision as of 14:17, 24 January 2024
Line 3:		Line 3:
	<SX load='3j80' size='340' side='right' viewer='molstar' caption='[[3j80]], [[Resolution\|resolution]] 3.75Å' scene=''>		<SX load='3j80' size='340' side='right' viewer='molstar' caption='[[3j80]], [[Resolution\|resolution]] 3.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3j80]] is a 37 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_56498 Atcc 56498~~] and [https://en.wikipedia.org/wiki/~~Kluyveromyces_lactis Kluyveromyces lactis~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J80 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J80 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3j80]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Kluyveromyces_lactis Kluyveromyces lactis] and [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J80 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J80 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~, ~~<scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.75Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height~~: ~~3em;~~'>~~[[3j81\|3j81]]~~</~~div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j80 OCA], [https://pdbe.org/3j80 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j80 RCSB], [https://www.ebi.ac.uk/pdbsum/3j80 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j80 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j80 OCA], [https://pdbe.org/3j80 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j80 RCSB], [https://www.ebi.ac.uk/pdbsum/3j80 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j80 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/RS27A_KLULA RS27A_KLULA]] Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, and DNA-damage responses. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling (By similarity). Ribosomal protein S27a is a component of the 40S subunit of the ribosome. [[https://www.uniprot.org/uniprot/RSSA_KLULA RSSA_KLULA]] Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. [[https://www.uniprot.org/uniprot/RS21_KLULA RS21_KLULA]] Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Has a physiological role leading to 18S rRNA stability (By similarity). [[https://www.uniprot.org/uniprot/IF1A_YEAST IF1A_YEAST]] Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits. [[https://www.uniprot.org/uniprot/SUI1_YEAST SUI1_YEAST]] Additional factor that functions in concert with eIF-2 and the initiator tRNA in directing the ribosome to the proper start site of translation.	+	[https://www.uniprot.org/uniprot/RS21_KLULA RS21_KLULA] Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Has a physiological role leading to 18S rRNA stability (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 26:		Line 26:
	__TOC__		__TOC__
	</SX>		</SX>
-	[[Category: Atcc 56498]]
	[[Category: Kluyveromyces lactis]]		[[Category: Kluyveromyces lactis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Fernandez~~, I S~~]]	+	[[Category: Saccharomyces cerevisiae]]
-	[[Category: Hussain, T]]	+	[[Category: Fernandez IS]]
-	[[Category: Llacer~~, J L~~]]	+	[[Category: Hussain T]]
-	[[Category: Ramakrishnan, V]]	+	[[Category: Llacer JL]]
-	[[Category: Savva~~, C G]]~~	+	[[Category: Ramakrishnan V]]
-	~~[[Category: Eukaryotic translation initiation]]~~	+	[[Category: Savva CG]]
-	~~[[Category: Ribosome]]~~	+
-	~~[[Category: Small ribosome subunit~~]]	+

OCA at 03:51, 21 April 2022

OCA — Thu, 21 Apr 2022 03:51:47 GMT

←Older revision		Revision as of 03:51, 21 April 2022
Line 3:		Line 3:
	<SX load='3j80' size='340' side='right' viewer='molstar' caption='[[3j80]], [[Resolution\|resolution]] 3.75Å' scene=''>		<SX load='3j80' size='340' side='right' viewer='molstar' caption='[[3j80]], [[Resolution\|resolution]] 3.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3j80]] is a 37 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_56498 Atcc 56498] and [~~http~~://en.wikipedia.org/wiki/Kluyveromyces_lactis Kluyveromyces lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J80 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=3J80 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3j80]] is a 37 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_56498 Atcc 56498] and [https://en.wikipedia.org/wiki/Kluyveromyces_lactis Kluyveromyces lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J80 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J80 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3j81\|3j81]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3j81\|3j81]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=3j80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j80 OCA], [~~http~~://pdbe.org/3j80 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3j80 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3j80 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3j80 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j80 OCA], [https://pdbe.org/3j80 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j80 RCSB], [https://www.ebi.ac.uk/pdbsum/3j80 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j80 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/RS27A_KLULA RS27A_KLULA]] Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, and DNA-damage responses. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling (By similarity). Ribosomal protein S27a is a component of the 40S subunit of the ribosome. [[~~http~~://www.uniprot.org/uniprot/RSSA_KLULA RSSA_KLULA]] Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. [[~~http~~://www.uniprot.org/uniprot/RS21_KLULA RS21_KLULA]] Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Has a physiological role leading to 18S rRNA stability (By similarity). [[~~http~~://www.uniprot.org/uniprot/IF1A_YEAST IF1A_YEAST]] Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits. [[~~http~~://www.uniprot.org/uniprot/SUI1_YEAST SUI1_YEAST]] Additional factor that functions in concert with eIF-2 and the initiator tRNA in directing the ribosome to the proper start site of translation.	+	[[https://www.uniprot.org/uniprot/RS27A_KLULA RS27A_KLULA]] Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, and DNA-damage responses. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling (By similarity). Ribosomal protein S27a is a component of the 40S subunit of the ribosome. [[https://www.uniprot.org/uniprot/RSSA_KLULA RSSA_KLULA]] Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. [[https://www.uniprot.org/uniprot/RS21_KLULA RS21_KLULA]] Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Has a physiological role leading to 18S rRNA stability (By similarity). [[https://www.uniprot.org/uniprot/IF1A_YEAST IF1A_YEAST]] Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits. [[https://www.uniprot.org/uniprot/SUI1_YEAST SUI1_YEAST]] Additional factor that functions in concert with eIF-2 and the initiator tRNA in directing the ribosome to the proper start site of translation.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 18:07, 10 April 2020

OCA — Fri, 10 Apr 2020 18:07:37 GMT

←Older revision		Revision as of 18:07, 10 April 2020
Line 3:		Line 3:
	<SX load='3j80' size='340' side='right' viewer='molstar' caption='[[3j80]], [[Resolution\|resolution]] 3.75Å' scene=''>		<SX load='3j80' size='340' side='right' viewer='molstar' caption='[[3j80]], [[Resolution\|resolution]] 3.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3j80]] is a 37 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_56498 Atcc 56498] and [http://en.wikipedia.org/wiki/Kluyveromyces_lactis Kluyveromyces lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J80 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3J80 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3j80]] is a 37 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_56498 Atcc 56498] and [http://en.wikipedia.org/wiki/Kluyveromyces_lactis Kluyveromyces lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J80 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3J80 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3j81\|3j81]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3j81\|3j81]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3j80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j80 OCA], [http://pdbe.org/3j80 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3j80 RCSB], [http://www.ebi.ac.uk/pdbsum/3j80 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3j80 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3j80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j80 OCA], [http://pdbe.org/3j80 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3j80 RCSB], [http://www.ebi.ac.uk/pdbsum/3j80 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3j80 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 17:29, 6 March 2020

OCA — Fri, 06 Mar 2020 17:29:05 GMT

←Older revision		Revision as of 17:29, 6 March 2020
Line 1:		Line 1:
-	~~{{Large structure}}~~	+
	==CryoEM structure of 40S-eIF1-eIF1A preinitiation complex==		==CryoEM structure of 40S-eIF1-eIF1A preinitiation complex==
-	<~~StructureSection~~ load='3j80' size='340' side='right' caption='[[3j80]], [[Resolution\|resolution]] 3.75Å' scene=''>	+	<SX load='3j80' size='340' side='right' viewer='molstar' caption='[[3j80]], [[Resolution\|resolution]] 3.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3j80]] is a 37 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_56498 Atcc 56498] and [http://en.wikipedia.org/wiki/Kluyveromyces_lactis Kluyveromyces lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J80 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J80 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3j80]] is a 37 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_56498 Atcc 56498] and [http://en.wikipedia.org/wiki/Kluyveromyces_lactis Kluyveromyces lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J80 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J80 FirstGlance]. <br>
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j80 OCA], [http://pdbe.org/3j80 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3j80 RCSB], [http://www.ebi.ac.uk/pdbsum/3j80 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3j80 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j80 OCA], [http://pdbe.org/3j80 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3j80 RCSB], [http://www.ebi.ac.uk/pdbsum/3j80 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3j80 ProSAT]</span></td></tr>
	</table>		</table>
-	{{Large structure}}
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/RS27A_KLULA RS27A_KLULA]] Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, and DNA-damage responses. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling (By similarity). Ribosomal protein S27a is a component of the 40S subunit of the ribosome. [[http://www.uniprot.org/uniprot/RSSA_KLULA RSSA_KLULA]] Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. [[http://www.uniprot.org/uniprot/RS21_KLULA RS21_KLULA]] Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Has a physiological role leading to 18S rRNA stability (By similarity). [[http://www.uniprot.org/uniprot/IF1A_YEAST IF1A_YEAST]] Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits. [[http://www.uniprot.org/uniprot/SUI1_YEAST SUI1_YEAST]] Additional factor that functions in concert with eIF-2 and the initiator tRNA in directing the ribosome to the proper start site of translation.		[[http://www.uniprot.org/uniprot/RS27A_KLULA RS27A_KLULA]] Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, and DNA-damage responses. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling (By similarity). Ribosomal protein S27a is a component of the 40S subunit of the ribosome. [[http://www.uniprot.org/uniprot/RSSA_KLULA RSSA_KLULA]] Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. [[http://www.uniprot.org/uniprot/RS21_KLULA RS21_KLULA]] Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Has a physiological role leading to 18S rRNA stability (By similarity). [[http://www.uniprot.org/uniprot/IF1A_YEAST IF1A_YEAST]] Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits. [[http://www.uniprot.org/uniprot/SUI1_YEAST SUI1_YEAST]] Additional factor that functions in concert with eIF-2 and the initiator tRNA in directing the ribosome to the proper start site of translation.
Line 22:		Line 21:

	==See Also==		==See Also==
-	*[[Eukaryotic initiation factor\|Eukaryotic initiation factor]]	+	*[[Eukaryotic initiation factor 3D structures\|Eukaryotic initiation factor 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Atcc 56498]]		[[Category: Atcc 56498]]
	[[Category: Kluyveromyces lactis]]		[[Category: Kluyveromyces lactis]]
		+	[[Category: Large Structures]]
	[[Category: Fernandez, I S]]		[[Category: Fernandez, I S]]
	[[Category: Hussain, T]]		[[Category: Hussain, T]]

OCA at 08:16, 18 July 2018

OCA — Wed, 18 Jul 2018 08:16:54 GMT

←Older revision		Revision as of 08:16, 18 July 2018
Line 3:		Line 3:
	<StructureSection load='3j80' size='340' side='right' caption='[[3j80]], [[Resolution\|resolution]] 3.75Å' scene=''>		<StructureSection load='3j80' size='340' side='right' caption='[[3j80]], [[Resolution\|resolution]] 3.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3j80]] is a 37 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Kluyveromyces_lactis Kluyveromyces lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J80 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J80 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3j80]] is a 37 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_56498 Atcc 56498] and [http://en.wikipedia.org/wiki/Kluyveromyces_lactis Kluyveromyces lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J80 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J80 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3j81\|3j81]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3j81\|3j81]]</td></tr>
Line 27:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 56498]]
	[[Category: Kluyveromyces lactis]]		[[Category: Kluyveromyces lactis]]
	[[Category: Fernandez, I S]]		[[Category: Fernandez, I S]]

OCA at 07:03, 5 August 2016

OCA — Fri, 05 Aug 2016 07:03:18 GMT

←Older revision		Revision as of 07:03, 5 August 2016
Line 1:		Line 1:
		+	{{Large structure}}
	==CryoEM structure of 40S-eIF1-eIF1A preinitiation complex==		==CryoEM structure of 40S-eIF1-eIF1A preinitiation complex==
	<StructureSection load='3j80' size='340' side='right' caption='[[3j80]], [[Resolution\|resolution]] 3.75Å' scene=''>		<StructureSection load='3j80' size='340' side='right' caption='[[3j80]], [[Resolution\|resolution]] 3.75Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3j81\|3j81]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3j81\|3j81]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j80 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3j80 RCSB], [http://www.ebi.ac.uk/pdbsum/3j80 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j80 OCA], [http://pdbe.org/3j80 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3j80 RCSB], [http://www.ebi.ac.uk/pdbsum/3j80 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3j80 ProSAT]</span></td></tr>
	</table>		</table>
		+	{{Large structure}}
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/RS27A_KLULA RS27A_KLULA]] Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, and DNA-damage responses. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling (By similarity). Ribosomal protein S27a is a component of the 40S subunit of the ribosome. [[http://www.uniprot.org/uniprot/RSSA_KLULA RSSA_KLULA]] Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. [[http://www.uniprot.org/uniprot/RS21_KLULA RS21_KLULA]] Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Has a physiological role leading to 18S rRNA stability (By similarity). [[http://www.uniprot.org/uniprot/IF1A_YEAST IF1A_YEAST]] Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits. [[http://www.uniprot.org/uniprot/SUI1_YEAST SUI1_YEAST]] Additional factor that functions in concert with eIF-2 and the initiator tRNA in directing the ribosome to the proper start site of translation.		[[http://www.uniprot.org/uniprot/RS27A_KLULA RS27A_KLULA]] Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, and DNA-damage responses. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling (By similarity). Ribosomal protein S27a is a component of the 40S subunit of the ribosome. [[http://www.uniprot.org/uniprot/RSSA_KLULA RSSA_KLULA]] Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. [[http://www.uniprot.org/uniprot/RS21_KLULA RS21_KLULA]] Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Has a physiological role leading to 18S rRNA stability (By similarity). [[http://www.uniprot.org/uniprot/IF1A_YEAST IF1A_YEAST]] Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits. [[http://www.uniprot.org/uniprot/SUI1_YEAST SUI1_YEAST]] Additional factor that functions in concert with eIF-2 and the initiator tRNA in directing the ribosome to the proper start site of translation.
Line 17:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3j80" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Eukaryotic initiation factor\|Eukaryotic initiation factor]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:50, 24 December 2014

OCA — Wed, 24 Dec 2014 10:50:36 GMT

←Older revision		Revision as of 10:50, 24 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j80 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3j80 RCSB], [http://www.ebi.ac.uk/pdbsum/3j80 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j80 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3j80 RCSB], [http://www.ebi.ac.uk/pdbsum/3j80 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/RS27A_KLULA RS27A_KLULA]] Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, and DNA-damage responses. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling (By similarity). Ribosomal protein S27a is a component of the 40S subunit of the ribosome. [[http://www.uniprot.org/uniprot/RSSA_KLULA RSSA_KLULA]] Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. [[http://www.uniprot.org/uniprot/RS21_KLULA RS21_KLULA]] Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Has a physiological role leading to 18S rRNA stability (By similarity). [[http://www.uniprot.org/uniprot/IF1A_YEAST IF1A_YEAST]] Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits. [[http://www.uniprot.org/uniprot/SUI1_YEAST SUI1_YEAST]] Additional factor that functions in concert with eIF-2 and the initiator tRNA in directing the ribosome to the proper start site of translation.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	During eukaryotic translation initiation, initiator tRNA does not insert fully into the P decoding site on the 40S ribosomal subunit. This conformation (POUT) is compatible with scanning mRNA for the AUG start codon. Base pairing with AUG is thought to promote isomerization to a more stable conformation (PIN) that arrests scanning and promotes dissociation of eIF1 from the 40S subunit. Here, we present a cryoEM reconstruction of a yeast preinitiation complex at 4.0 A resolution with initiator tRNA in the PIN state, prior to eIF1 release. The structure reveals stabilization of the codon-anticodon duplex by the N-terminal tail of eIF1A, changes in the structure of eIF1 likely instrumental in its subsequent release, and changes in the conformation of eIF2. The mRNA traverses the entire mRNA cleft and makes connections to the regulatory domain of eIF2?, eIF1A, and ribosomal elements that allow recognition of context nucleotides surrounding the AUG codon.
		+
		+	Structural changes enable start codon recognition by the eukaryotic translation initiation complex.,Hussain T, Llacer JL, Fernandez IS, Munoz A, Martin-Marcos P, Savva CG, Lorsch JR, Hinnebusch AG, Ramakrishnan V Cell. 2014 Oct 23;159(3):597-607. doi: 10.1016/j.cell.2014.10.001. Epub 2014 Oct , 16. PMID:25417110<ref>PMID:25417110</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Kluyveromyces lactis]]		[[Category: Kluyveromyces lactis]]
-	[[Category: Fernandez, I S.]]	+	[[Category: Fernandez, I S]]
-	[[Category: Hussain, T.]]	+	[[Category: Hussain, T]]
-	[[Category: Llacer, J L.]]	+	[[Category: Llacer, J L]]
-	[[Category: Ramakrishnan, V.]]	+	[[Category: Ramakrishnan, V]]
-	[[Category: Savva, C G.]]	+	[[Category: Savva, C G]]
	[[Category: Eukaryotic translation initiation]]		[[Category: Eukaryotic translation initiation]]
	[[Category: Ribosome]]		[[Category: Ribosome]]
	[[Category: Small ribosome subunit]]		[[Category: Small ribosome subunit]]

OCA at 08:53, 5 November 2014

OCA — Wed, 05 Nov 2014 08:53:07 GMT

←Older revision		Revision as of 08:53, 5 November 2014
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==CryoEM structure of 40S-eIF1-eIF1A preinitiation complex==
-		+	<StructureSection load='3j80' size='340' side='right' caption='[[3j80]], [[Resolution\|resolution]] 3.75Å' scene=''>
-	~~The entry~~ 3j80 is ~~ON HOLD until Paper Publication~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[3j80]] is a 37 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Kluyveromyces_lactis Kluyveromyces lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J80 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J80 FirstGlance]. <br>
-	~~Authors~~: ~~Hussain~~, T., ~~Llacer, J~~.L., Fernandez, I.S., ~~Savva, C.G~~., Ramakrishnan, V.	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3j81\|3j81]]</td></tr>
-	~~Description~~: ~~CryoEM structure of 40S-eIF1-eIF1A preinitiation complex~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j80 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3j80 RCSB], [http://www.ebi.ac.uk/pdbsum/3j80 PDBsum]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Kluyveromyces lactis]]
		+	[[Category: Fernandez, I S.]]
		+	[[Category: Hussain, T.]]
		+	[[Category: Llacer, J L.]]
		+	[[Category: Ramakrishnan, V.]]
		+	[[Category: Savva, C G.]]
		+	[[Category: Eukaryotic translation initiation]]
		+	[[Category: Ribosome]]
		+	[[Category: Small ribosome subunit]]

OCA at 08:59, 20 October 2014

OCA — Mon, 20 Oct 2014 08:59:18 GMT

←Older revision		Revision as of 08:59, 20 October 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3j80 is ON HOLD	+	The entry 3j80 is ON HOLD until Paper Publication

	Authors: Hussain, T., Llacer, J.L., Fernandez, I.S., Savva, C.G., Ramakrishnan, V.		Authors: Hussain, T., Llacer, J.L., Fernandez, I.S., Savva, C.G., Ramakrishnan, V.

	Description: CryoEM structure of 40S-eIF1-eIF1A preinitiation complex		Description: CryoEM structure of 40S-eIF1-eIF1A preinitiation complex