3j96 - Revision history

OCA at 08:41, 7 February 2024

OCA — Wed, 07 Feb 2024 08:41:04 GMT

←Older revision		Revision as of 08:41, 7 February 2024
Line 3:		Line 3:
	<SX load='3j96' size='340' side='right' viewer='molstar' caption='[[3j96]], [[Resolution\|resolution]] 7.60Å' scene=''>		<SX load='3j96' size='340' side='right' viewer='molstar' caption='[[3j96]], [[Resolution\|resolution]] 7.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3j96]] is a 13 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Buffalo_rat Buffalo rat~~] and [https://en.wikipedia.org/wiki/~~Cho_cell_lines Cho cell lines~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J96 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J96 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3j96]] is a 13 chain structure with sequence from [https://en.wikipedia.org/wiki/Cricetulus_griseus Cricetulus griseus] and [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J96 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J96 FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[3j94\|3j94]], [[3j95\|3j95]], [[3j97\|3j97]], [[3j98\|3j98]], [[3j99\|3j99]]</div></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 7.601Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">NSF ([https://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&srchmode=5&id=10029 CHO cell lines]), Napa, Snap, Snapa ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat]), Vamp2, Syb2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat]), Stx1a, Sap ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat]), Snap25, Snap ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Vesicle-fusing_ATPase Vesicle-fusing ATPase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.6 3.6.4.6] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j96 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j96 OCA], [https://pdbe.org/3j96 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j96 RCSB], [https://www.ebi.ac.uk/pdbsum/3j96 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j96 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j96 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j96 OCA], [https://pdbe.org/3j96 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j96 RCSB], [https://www.ebi.ac.uk/pdbsum/3j96 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j96 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/STX1A_RAT STX1A_RAT]] Potentially involved in docking of synaptic vesicles at presynaptic active zones. May play a critical role in neurotransmitter exocytosis. May mediate Ca(2+)-regulation of exocytosis acrosomal reaction in sperm. [[https://www.uniprot.org/uniprot/NSF_CRIGR NSF_CRIGR]] Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling. [[https://www.uniprot.org/uniprot/VAMP2_RAT VAMP2_RAT]] Involved in the targeting and/or fusion of transport vesicles to their target membrane (By similarity). [[https://www.uniprot.org/uniprot/SNAA_RAT SNAA_RAT]] Required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus.	+	[https://www.uniprot.org/uniprot/NSF_CRIGR NSF_CRIGR] Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling.
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Evolutionarily conserved SNARE (soluble N-ethylmaleimide sensitive factor attachment protein receptors) proteins form a complex that drives membrane fusion in eukaryotes. The ATPase NSF (N-ethylmaleimide sensitive factor), together with SNAPs (soluble NSF attachment protein), disassembles the SNARE complex into its protein components, making individual SNAREs available for subsequent rounds of fusion. Here we report structures of ATP- and ADP-bound NSF, and the NSF/SNAP/SNARE (20S) supercomplex determined by single-particle electron cryomicroscopy at near-atomic to sub-nanometre resolution without imposing symmetry. Large, potentially force-generating, conformational differences exist between ATP- and ADP-bound NSF. The 20S supercomplex exhibits broken symmetry, transitioning from six-fold symmetry of the NSF ATPase domains to pseudo four-fold symmetry of the SNARE complex. SNAPs interact with the SNARE complex with an opposite structural twist, suggesting an unwinding mechanism. The interfaces between NSF, SNAPs, and SNAREs exhibit characteristic electrostatic patterns, suggesting how one NSF/SNAP species can act on many different SNARE complexes.	+
-		+
-	Mechanistic insights into the recycling machine of the SNARE complex.,Zhao M, Wu S, Zhou Q, Vivona S, Cipriano DJ, Cheng Y, Brunger AT Nature. 2015 Jan 12. doi: 10.1038/nature14148. PMID:25581794<ref>PMID:25581794</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3j96" style="background-color:#fffaf0;"></div>~~	+

	==See Also==		==See Also==
Line 25:		Line 14:
	*[[Syntaxin 3D structures\|Syntaxin 3D structures]]		*[[Syntaxin 3D structures\|Syntaxin 3D structures]]
	*[[Vesicle-associated membrane protein\|Vesicle-associated membrane protein]]		*[[Vesicle-associated membrane protein\|Vesicle-associated membrane protein]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</SX>		</SX>
-	[[Category: ~~Buffalo rat]]~~	+	[[Category: Cricetulus griseus]]
-	~~[[Category: Cho cell lines~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Vesicle-fusing ATPase~~]]	+	[[Category: Rattus norvegicus]]
-	[[Category: Brunger~~, A T~~]]	+	[[Category: Brunger AT]]
-	[[Category: Cheng, Y]]	+	[[Category: Cheng Y]]
-	[[Category: Wu, S]]	+	[[Category: Wu S]]
-	[[Category: Zhao, M]]	+	[[Category: Zhao M]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Vesicle trafficking~~]]	+

OCA at 13:28, 4 May 2022

OCA — Wed, 04 May 2022 13:28:33 GMT

←Older revision		Revision as of 13:28, 4 May 2022
Line 3:		Line 3:
	<SX load='3j96' size='340' side='right' viewer='molstar' caption='[[3j96]], [[Resolution\|resolution]] 7.60Å' scene=''>		<SX load='3j96' size='340' side='right' viewer='molstar' caption='[[3j96]], [[Resolution\|resolution]] 7.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3j96]] is a 13 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat] and [~~http~~://en.wikipedia.org/wiki/Cho_cell_lines Cho cell lines]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J96 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=3J96 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3j96]] is a 13 chain structure with sequence from [https://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat] and [https://en.wikipedia.org/wiki/Cho_cell_lines Cho cell lines]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J96 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J96 FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3j94\|3j94]], [[3j95\|3j95]], [[3j97\|3j97]], [[3j98\|3j98]], [[3j99\|3j99]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3j94\|3j94]], [[3j95\|3j95]], [[3j97\|3j97]], [[3j98\|3j98]], [[3j99\|3j99]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NSF ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10029 CHO cell lines]), Napa, Snap, Snapa ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat]), Vamp2, Syb2 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat]), Stx1a, Sap ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat]), Snap25, Snap ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NSF ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10029 CHO cell lines]), Napa, Snap, Snapa ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat]), Vamp2, Syb2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat]), Stx1a, Sap ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat]), Snap25, Snap ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Vesicle-fusing_ATPase Vesicle-fusing ATPase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.6 3.6.4.6] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Vesicle-fusing_ATPase Vesicle-fusing ATPase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.6 3.6.4.6] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=3j96 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j96 OCA], [~~http~~://pdbe.org/3j96 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3j96 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3j96 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3j96 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j96 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j96 OCA], [https://pdbe.org/3j96 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j96 RCSB], [https://www.ebi.ac.uk/pdbsum/3j96 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j96 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/STX1A_RAT STX1A_RAT]] Potentially involved in docking of synaptic vesicles at presynaptic active zones. May play a critical role in neurotransmitter exocytosis. May mediate Ca(2+)-regulation of exocytosis acrosomal reaction in sperm. [[~~http~~://www.uniprot.org/uniprot/NSF_CRIGR NSF_CRIGR]] Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling. [[~~http~~://www.uniprot.org/uniprot/VAMP2_RAT VAMP2_RAT]] Involved in the targeting and/or fusion of transport vesicles to their target membrane (By similarity). [[~~http~~://www.uniprot.org/uniprot/SNAA_RAT SNAA_RAT]] Required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus.	+	[[https://www.uniprot.org/uniprot/STX1A_RAT STX1A_RAT]] Potentially involved in docking of synaptic vesicles at presynaptic active zones. May play a critical role in neurotransmitter exocytosis. May mediate Ca(2+)-regulation of exocytosis acrosomal reaction in sperm. [[https://www.uniprot.org/uniprot/NSF_CRIGR NSF_CRIGR]] Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling. [[https://www.uniprot.org/uniprot/VAMP2_RAT VAMP2_RAT]] Involved in the targeting and/or fusion of transport vesicles to their target membrane (By similarity). [[https://www.uniprot.org/uniprot/SNAA_RAT SNAA_RAT]] Required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 18:12, 10 April 2020

OCA — Fri, 10 Apr 2020 18:12:20 GMT

←Older revision		Revision as of 18:12, 10 April 2020
Line 3:		Line 3:
	<SX load='3j96' size='340' side='right' viewer='molstar' caption='[[3j96]], [[Resolution\|resolution]] 7.60Å' scene=''>		<SX load='3j96' size='340' side='right' viewer='molstar' caption='[[3j96]], [[Resolution\|resolution]] 7.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3j96]] is a 13 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat] and [http://en.wikipedia.org/wiki/Cho_cell_lines Cho cell lines]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J96 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3J96 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3j96]] is a 13 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat] and [http://en.wikipedia.org/wiki/Cho_cell_lines Cho cell lines]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J96 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3J96 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3j94\|3j94]], [[3j95\|3j95]], [[3j97\|3j97]], [[3j98\|3j98]], [[3j99\|3j99]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3j94\|3j94]], [[3j95\|3j95]], [[3j97\|3j97]], [[3j98\|3j98]], [[3j99\|3j99]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NSF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10029 CHO cell lines]), Napa, Snap, Snapa ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat]), Vamp2, Syb2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat]), Stx1a, Sap ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat]), Snap25, Snap ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NSF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10029 CHO cell lines]), Napa, Snap, Snapa ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat]), Vamp2, Syb2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat]), Stx1a, Sap ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat]), Snap25, Snap ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Vesicle-fusing_ATPase Vesicle-fusing ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.6 3.6.4.6] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Vesicle-fusing_ATPase Vesicle-fusing ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.6 3.6.4.6] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3j96 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j96 OCA], [http://pdbe.org/3j96 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3j96 RCSB], [http://www.ebi.ac.uk/pdbsum/3j96 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3j96 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3j96 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j96 OCA], [http://pdbe.org/3j96 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3j96 RCSB], [http://www.ebi.ac.uk/pdbsum/3j96 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3j96 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 17:31, 6 March 2020

OCA — Fri, 06 Mar 2020 17:31:18 GMT

←Older revision		Revision as of 17:31, 6 March 2020
Line 1:		Line 1:

	==Structure of 20S supercomplex determined by single particle cryoelectron microscopy (State I)==		==Structure of 20S supercomplex determined by single particle cryoelectron microscopy (State I)==
-	<~~StructureSection~~ load='3j96' size='340' side='right' caption='[[3j96]], [[Resolution\|resolution]] 7.60Å' scene=''>	+	<SX load='3j96' size='340' side='right' viewer='molstar' caption='[[3j96]], [[Resolution\|resolution]] 7.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3j96]] is a 13 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat] and [http://en.wikipedia.org/wiki/Cho_cell_lines Cho cell lines]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J96 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J96 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3j96]] is a 13 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat] and [http://en.wikipedia.org/wiki/Cho_cell_lines Cho cell lines]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J96 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J96 FirstGlance]. <br>
Line 20:		Line 20:
	</div>		</div>
	<div class="pdbe-citations 3j96" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3j96" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Synaptosomal-associated protein\|Synaptosomal-associated protein]]
		+	*[[Syntaxin 3D structures\|Syntaxin 3D structures]]
		+	*[[Vesicle-associated membrane protein\|Vesicle-associated membrane protein]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Buffalo rat]]		[[Category: Buffalo rat]]
	[[Category: Cho cell lines]]		[[Category: Cho cell lines]]
		+	[[Category: Large Structures]]
	[[Category: Vesicle-fusing ATPase]]		[[Category: Vesicle-fusing ATPase]]
	[[Category: Brunger, A T]]		[[Category: Brunger, A T]]

OCA at 08:18, 18 July 2018

OCA — Wed, 18 Jul 2018 08:18:25 GMT

←Older revision		Revision as of 08:18, 18 July 2018
Line 3:		Line 3:
	<StructureSection load='3j96' size='340' side='right' caption='[[3j96]], [[Resolution\|resolution]] 7.60Å' scene=''>		<StructureSection load='3j96' size='340' side='right' caption='[[3j96]], [[Resolution\|resolution]] 7.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3j96]] is a 13 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J96 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J96 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3j96]] is a 13 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat] and [http://en.wikipedia.org/wiki/Cho_cell_lines Cho cell lines]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J96 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J96 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3j94\|3j94]], [[3j95\|3j95]], [[3j97\|3j97]], [[3j98\|3j98]], [[3j99\|3j99]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3j94\|3j94]], [[3j95\|3j95]], [[3j97\|3j97]], [[3j98\|3j98]], [[3j99\|3j99]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NSF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10029 CHO cell lines]), Napa, Snap, Snapa ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat]), Vamp2, Syb2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat]), Stx1a, Sap ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat]), Snap25, Snap ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Vesicle-fusing_ATPase Vesicle-fusing ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.6 3.6.4.6] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Vesicle-fusing_ATPase Vesicle-fusing ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.6 3.6.4.6] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j96 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j96 OCA], [http://pdbe.org/3j96 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3j96 RCSB], [http://www.ebi.ac.uk/pdbsum/3j96 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3j96 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j96 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j96 OCA], [http://pdbe.org/3j96 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3j96 RCSB], [http://www.ebi.ac.uk/pdbsum/3j96 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3j96 ProSAT]</span></td></tr>
Line 23:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Buffalo rat]]
		+	[[Category: Cho cell lines]]
	[[Category: Vesicle-fusing ATPase]]		[[Category: Vesicle-fusing ATPase]]
	[[Category: Brunger, A T]]		[[Category: Brunger, A T]]

OCA at 20:17, 9 December 2016

OCA — Fri, 09 Dec 2016 20:17:51 GMT

←Older revision		Revision as of 20:17, 9 December 2016
Line 1:		Line 1:
		+
	==Structure of 20S supercomplex determined by single particle cryoelectron microscopy (State I)==		==Structure of 20S supercomplex determined by single particle cryoelectron microscopy (State I)==
	<StructureSection load='3j96' size='340' side='right' caption='[[3j96]], [[Resolution\|resolution]] 7.60Å' scene=''>		<StructureSection load='3j96' size='340' side='right' caption='[[3j96]], [[Resolution\|resolution]] 7.60Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3j94\|3j94]], [[3j95\|3j95]], [[3j97\|3j97]], [[3j98\|3j98]], [[3j99\|3j99]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3j94\|3j94]], [[3j95\|3j95]], [[3j97\|3j97]], [[3j98\|3j98]], [[3j99\|3j99]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Vesicle-fusing_ATPase Vesicle-fusing ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.6 3.6.4.6] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Vesicle-fusing_ATPase Vesicle-fusing ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.6 3.6.4.6] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j96 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j96 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3j96 RCSB], [http://www.ebi.ac.uk/pdbsum/3j96 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j96 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j96 OCA], [http://pdbe.org/3j96 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3j96 RCSB], [http://www.ebi.ac.uk/pdbsum/3j96 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3j96 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3j96" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 16:33, 28 January 2015

OCA — Wed, 28 Jan 2015 16:33:09 GMT

←Older revision		Revision as of 16:33, 28 January 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Structure of 20S supercomplex determined by single particle cryoelectron microscopy (State I)==
		+	<StructureSection load='3j96' size='340' side='right' caption='[[3j96]], [[Resolution\|resolution]] 7.60Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3j96]] is a 13 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J96 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J96 FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3j94\|3j94]], [[3j95\|3j95]], [[3j97\|3j97]], [[3j98\|3j98]], [[3j99\|3j99]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Vesicle-fusing_ATPase Vesicle-fusing ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.6 3.6.4.6] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j96 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j96 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3j96 RCSB], [http://www.ebi.ac.uk/pdbsum/3j96 PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/STX1A_RAT STX1A_RAT]] Potentially involved in docking of synaptic vesicles at presynaptic active zones. May play a critical role in neurotransmitter exocytosis. May mediate Ca(2+)-regulation of exocytosis acrosomal reaction in sperm. [[http://www.uniprot.org/uniprot/NSF_CRIGR NSF_CRIGR]] Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling. [[http://www.uniprot.org/uniprot/VAMP2_RAT VAMP2_RAT]] Involved in the targeting and/or fusion of transport vesicles to their target membrane (By similarity). [[http://www.uniprot.org/uniprot/SNAA_RAT SNAA_RAT]] Required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Evolutionarily conserved SNARE (soluble N-ethylmaleimide sensitive factor attachment protein receptors) proteins form a complex that drives membrane fusion in eukaryotes. The ATPase NSF (N-ethylmaleimide sensitive factor), together with SNAPs (soluble NSF attachment protein), disassembles the SNARE complex into its protein components, making individual SNAREs available for subsequent rounds of fusion. Here we report structures of ATP- and ADP-bound NSF, and the NSF/SNAP/SNARE (20S) supercomplex determined by single-particle electron cryomicroscopy at near-atomic to sub-nanometre resolution without imposing symmetry. Large, potentially force-generating, conformational differences exist between ATP- and ADP-bound NSF. The 20S supercomplex exhibits broken symmetry, transitioning from six-fold symmetry of the NSF ATPase domains to pseudo four-fold symmetry of the SNARE complex. SNAPs interact with the SNARE complex with an opposite structural twist, suggesting an unwinding mechanism. The interfaces between NSF, SNAPs, and SNAREs exhibit characteristic electrostatic patterns, suggesting how one NSF/SNAP species can act on many different SNARE complexes.

-	~~The entry 3j96 is ON HOLD~~	+	Mechanistic insights into the recycling machine of the SNARE complex.,Zhao M, Wu S, Zhou Q, Vivona S, Cipriano DJ, Cheng Y, Brunger AT Nature. 2015 Jan 12. doi: 10.1038/nature14148. PMID:25581794<ref>PMID:25581794</ref>

-	~~Authors: Zhao~~, M.~~, Wu,~~ S.~~, Cheng, Y., Brunger, A.T~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~Structure of 20S supercomplex determined by single particle cryoelectron microscopy (State I)~~	+	== References ==
-	[[Category: ~~Unreleased Structures~~]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Vesicle-fusing ATPase]]
		+	[[Category: Brunger, A T]]
	[[Category: Cheng, Y]]		[[Category: Cheng, Y]]
	[[Category: Wu, S]]		[[Category: Wu, S]]
	[[Category: Zhao, M]]		[[Category: Zhao, M]]
-	[[Category: ~~Brunger, A.T~~]]	+	[[Category: Hydrolase]]
		+	[[Category: Vesicle trafficking]]

OCA at 12:42, 24 December 2014

OCA — Wed, 24 Dec 2014 12:42:50 GMT

←Older revision		Revision as of 12:42, 24 December 2014
Line 6:		Line 6:

	Description: Structure of 20S supercomplex determined by single particle cryoelectron microscopy (State I)		Description: Structure of 20S supercomplex determined by single particle cryoelectron microscopy (State I)
		+	[[Category: Unreleased Structures]]
		+	[[Category: Cheng, Y]]
		+	[[Category: Wu, S]]
		+	[[Category: Zhao, M]]
		+	[[Category: Brunger, A.T]]

OCA: Protected "3j96" [edit=sysop:move=sysop]

OCA — Wed, 10 Dec 2014 12:46:40 GMT

Protected "3j96" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:46, 10 December 2014

OCA: New page: '''Unreleased structure''' The entry 3j96 is ON HOLD Authors: Zhao, M., Wu, S., Cheng, Y., Brunger, A.T. Description: Structure of 20S supercomplex determined by single particle cryoel...

OCA — Wed, 10 Dec 2014 12:46:38 GMT

New page: '''Unreleased structure''' The entry 3j96 is ON HOLD Authors: Zhao, M., Wu, S., Cheng, Y., Brunger, A.T. Description: Structure of 20S supercomplex determined by single particle cryoel...

New page

'''Unreleased structure'''

The entry 3j96 is ON HOLD

Authors: Zhao, M., Wu, S., Cheng, Y., Brunger, A.T.

Description: Structure of 20S supercomplex determined by single particle cryoelectron microscopy (State I)