3j9k - Revision history

OCA at 08:41, 7 February 2024

OCA — Wed, 07 Feb 2024 08:41:21 GMT

←Older revision		Revision as of 08:41, 7 February 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3j9k]] is a 32 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J9K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J9K FirstGlance]. <br>		<table><tr><td colspan='2'>[[3j9k]] is a 32 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J9K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J9K FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 4.1Å</td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>~~[[~~Non-Standard_Residue~~\|~~NonStd Res:~~]]~~</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[3j9l\|3j9l]]~~</~~div~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j9k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j9k OCA], [https://pdbe.org/3j9k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j9k RCSB], [https://www.ebi.ac.uk/pdbsum/3j9k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j9k ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j9k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j9k OCA], [https://pdbe.org/3j9k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j9k RCSB], [https://www.ebi.ac.uk/pdbsum/3j9k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j9k ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~ICENC_DROME ICENC_DROME~~]] Involved in the activation cascade of caspases responsible for apoptosis execution. Effector of steroid-mediated apoptosis during insect metamorphosis. Overexpression promotes programmed cell death. Interaction with th is required to suppress Nc-mediated cell death; via th-mediated ubiquitination of Nc. Rate-limiting caspase in rpr and W death pathway.<ref>PMID:10200258</ref> <ref>PMID:10675329</ref> <ref>PMID:10984473</ref> <ref>PMID:14517550</ref>	+	[https://www.uniprot.org/uniprot/Q7KLI1_DROME Q7KLI1_DROME]
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Apoptosis is executed by a cascade of caspase activation. The autocatalytic activation of an initiator caspase, exemplified by caspase-9 in mammals or its ortholog, Dronc, in fruit flies, is facilitated by a multimeric adaptor complex known as the apoptosome. The underlying mechanism by which caspase-9 or Dronc is activated by the apoptosome remains unknown. Here we report the electron cryomicroscopic (cryo-EM) structure of the intact apoptosome from Drosophila melanogaster at 4.0 A resolution. Analysis of the Drosophila apoptosome, which comprises 16 molecules of the Dark protein (Apaf-1 ortholog), reveals molecular determinants that support the assembly of the 2.5-MDa complex. In the absence of dATP or ATP, Dronc zymogen potently induces formation of the Dark apoptosome, within which Dronc is efficiently activated. At 4.1 A resolution, the cryo-EM structure of the Dark apoptosome bound to the caspase recruitment domain (CARD) of Dronc (Dronc-CARD) reveals two stacked rings of Dronc-CARD that are sandwiched between two octameric rings of the Dark protein. The specific interactions between Dronc-CARD and both the CARD and the WD40 repeats of a nearby Dark protomer are indispensable for Dronc activation. These findings reveal important mechanistic insights into the activation of initiator caspase by the apoptosome.	+
-		+
-	Structure of the apoptosome: mechanistic insights into activation of an initiator caspase from Drosophila.,Pang Y, Bai XC, Yan C, Hao Q, Chen Z, Wang JW, Scheres SH, Shi Y Genes Dev. 2015 Feb 1;29(3):277-87. doi: 10.1101/gad.255877.114. PMID:25644603<ref>PMID:25644603</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3j9k" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</SX>		</SX>
	[[Category: Drosophila melanogaster]]		[[Category: Drosophila melanogaster]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bai, X]]	+	[[Category: Bai X]]
-	[[Category: Chen, Z]]	+	[[Category: Chen Z]]
-	[[Category: Hao, Q]]	+	[[Category: Hao Q]]
-	[[Category: Pang, Y]]	+	[[Category: Pang Y]]
-	[[Category: Scheres~~, S H.W~~]]	+	[[Category: Scheres SHW]]
-	[[Category: Shi, Y]]	+	[[Category: Shi Y]]
-	[[Category: Wang, J]]	+	[[Category: Wang J]]
-	[[Category: Yan, C]]	+	[[Category: Yan C]]
-	~~[[Category: Apoptosis]]~~	+
-	~~[[Category: Caspase activation]]~~	+
-	~~[[Category: Programmed cell death~~]]	+

OCA at 13:28, 4 May 2022

OCA — Wed, 04 May 2022 13:28:58 GMT

←Older revision		Revision as of 13:28, 4 May 2022
Line 3:		Line 3:
	<SX load='3j9k' size='340' side='right' viewer='molstar' caption='[[3j9k]], [[Resolution\|resolution]] 4.10Å' scene=''>		<SX load='3j9k' size='340' side='right' viewer='molstar' caption='[[3j9k]], [[Resolution\|resolution]] 4.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3j9k]] is a 32 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J9K OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=3J9K FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3j9k]] is a 32 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J9K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J9K FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3j9l\|3j9l]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3j9l\|3j9l]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=3j9k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j9k OCA], [~~http~~://pdbe.org/3j9k PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3j9k RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3j9k PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3j9k ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j9k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j9k OCA], [https://pdbe.org/3j9k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j9k RCSB], [https://www.ebi.ac.uk/pdbsum/3j9k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j9k ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/ICENC_DROME ICENC_DROME]] Involved in the activation cascade of caspases responsible for apoptosis execution. Effector of steroid-mediated apoptosis during insect metamorphosis. Overexpression promotes programmed cell death. Interaction with th is required to suppress Nc-mediated cell death; via th-mediated ubiquitination of Nc. Rate-limiting caspase in rpr and W death pathway.<ref>PMID:10200258</ref> <ref>PMID:10675329</ref> <ref>PMID:10984473</ref> <ref>PMID:14517550</ref>	+	[[https://www.uniprot.org/uniprot/ICENC_DROME ICENC_DROME]] Involved in the activation cascade of caspases responsible for apoptosis execution. Effector of steroid-mediated apoptosis during insect metamorphosis. Overexpression promotes programmed cell death. Interaction with th is required to suppress Nc-mediated cell death; via th-mediated ubiquitination of Nc. Rate-limiting caspase in rpr and W death pathway.<ref>PMID:10200258</ref> <ref>PMID:10675329</ref> <ref>PMID:10984473</ref> <ref>PMID:14517550</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 18:14, 10 April 2020

OCA — Fri, 10 Apr 2020 18:14:34 GMT

←Older revision		Revision as of 18:14, 10 April 2020
Line 3:		Line 3:
	<SX load='3j9k' size='340' side='right' viewer='molstar' caption='[[3j9k]], [[Resolution\|resolution]] 4.10Å' scene=''>		<SX load='3j9k' size='340' side='right' viewer='molstar' caption='[[3j9k]], [[Resolution\|resolution]] 4.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3j9k]] is a 32 chain structure with sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J9K OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3J9K FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3j9k]] is a 32 chain structure with sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J9K OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3J9K FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3j9l\|3j9l]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3j9l\|3j9l]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3j9k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j9k OCA], [http://pdbe.org/3j9k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3j9k RCSB], [http://www.ebi.ac.uk/pdbsum/3j9k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3j9k ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3j9k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j9k OCA], [http://pdbe.org/3j9k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3j9k RCSB], [http://www.ebi.ac.uk/pdbsum/3j9k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3j9k ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 17:32, 6 March 2020

OCA — Fri, 06 Mar 2020 17:32:23 GMT

←Older revision		Revision as of 17:32, 6 March 2020
Line 1:		Line 1:
-	~~{{Large structure}}~~	+
	==Structure of Dark apoptosome in complex with Dronc CARD domain==		==Structure of Dark apoptosome in complex with Dronc CARD domain==
-	<~~StructureSection~~ load='3j9k' size='340' side='right' caption='[[3j9k]], [[Resolution\|resolution]] 4.10Å' scene=''>	+	<SX load='3j9k' size='340' side='right' viewer='molstar' caption='[[3j9k]], [[Resolution\|resolution]] 4.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3j9k]] is a 32 chain structure with sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J9K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J9K FirstGlance]. <br>		<table><tr><td colspan='2'>[[3j9k]] is a 32 chain structure with sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J9K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J9K FirstGlance]. <br>
Line 9:		Line 9:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j9k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j9k OCA], [http://pdbe.org/3j9k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3j9k RCSB], [http://www.ebi.ac.uk/pdbsum/3j9k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3j9k ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j9k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j9k OCA], [http://pdbe.org/3j9k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3j9k RCSB], [http://www.ebi.ac.uk/pdbsum/3j9k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3j9k ProSAT]</span></td></tr>
	</table>		</table>
-	{{Large structure}}
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/ICENC_DROME ICENC_DROME]] Involved in the activation cascade of caspases responsible for apoptosis execution. Effector of steroid-mediated apoptosis during insect metamorphosis. Overexpression promotes programmed cell death. Interaction with th is required to suppress Nc-mediated cell death; via th-mediated ubiquitination of Nc. Rate-limiting caspase in rpr and W death pathway.<ref>PMID:10200258</ref> <ref>PMID:10675329</ref> <ref>PMID:10984473</ref> <ref>PMID:14517550</ref>		[[http://www.uniprot.org/uniprot/ICENC_DROME ICENC_DROME]] Involved in the activation cascade of caspases responsible for apoptosis execution. Effector of steroid-mediated apoptosis during insect metamorphosis. Overexpression promotes programmed cell death. Interaction with th is required to suppress Nc-mediated cell death; via th-mediated ubiquitination of Nc. Rate-limiting caspase in rpr and W death pathway.<ref>PMID:10200258</ref> <ref>PMID:10675329</ref> <ref>PMID:10984473</ref> <ref>PMID:14517550</ref>
Line 24:		Line 23:
	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Drosophila melanogaster]]		[[Category: Drosophila melanogaster]]
		+	[[Category: Large Structures]]
	[[Category: Bai, X]]		[[Category: Bai, X]]
	[[Category: Chen, Z]]		[[Category: Chen, Z]]

OCA at 08:19, 18 July 2018

OCA — Wed, 18 Jul 2018 08:19:04 GMT

←Older revision		Revision as of 08:19, 18 July 2018
Line 1:		Line 1:
		+	{{Large structure}}
	==Structure of Dark apoptosome in complex with Dronc CARD domain==		==Structure of Dark apoptosome in complex with Dronc CARD domain==
	<StructureSection load='3j9k' size='340' side='right' caption='[[3j9k]], [[Resolution\|resolution]] 4.10Å' scene=''>		<StructureSection load='3j9k' size='340' side='right' caption='[[3j9k]], [[Resolution\|resolution]] 4.10Å' scene=''>
Line 6:		Line 7:
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3j9l\|3j9l]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3j9l\|3j9l]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j9k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j9k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3j9k RCSB], [http://www.ebi.ac.uk/pdbsum/3j9k PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j9k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j9k OCA], [http://pdbe.org/3j9k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3j9k RCSB], [http://www.ebi.ac.uk/pdbsum/3j9k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3j9k ProSAT]</span></td></tr>
	</table>		</table>
	{{Large structure}}		{{Large structure}}
Line 19:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3j9k" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 13:46, 1 September 2015

OCA — Tue, 01 Sep 2015 13:46:30 GMT

←Older revision		Revision as of 13:46, 1 September 2015
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j9k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j9k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3j9k RCSB], [http://www.ebi.ac.uk/pdbsum/3j9k PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j9k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j9k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3j9k RCSB], [http://www.ebi.ac.uk/pdbsum/3j9k PDBsum]</span></td></tr>
	</table>		</table>
		+	{{Large structure}}
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/ICENC_DROME ICENC_DROME]] Involved in the activation cascade of caspases responsible for apoptosis execution. Effector of steroid-mediated apoptosis during insect metamorphosis. Overexpression promotes programmed cell death. Interaction with th is required to suppress Nc-mediated cell death; via th-mediated ubiquitination of Nc. Rate-limiting caspase in rpr and W death pathway.<ref>PMID:10200258</ref> <ref>PMID:10675329</ref> <ref>PMID:10984473</ref> <ref>PMID:14517550</ref>		[[http://www.uniprot.org/uniprot/ICENC_DROME ICENC_DROME]] Involved in the activation cascade of caspases responsible for apoptosis execution. Effector of steroid-mediated apoptosis during insect metamorphosis. Overexpression promotes programmed cell death. Interaction with th is required to suppress Nc-mediated cell death; via th-mediated ubiquitination of Nc. Rate-limiting caspase in rpr and W death pathway.<ref>PMID:10200258</ref> <ref>PMID:10675329</ref> <ref>PMID:10984473</ref> <ref>PMID:14517550</ref>

OCA at 13:57, 25 February 2015

OCA — Wed, 25 Feb 2015 13:57:42 GMT

←Older revision		Revision as of 13:57, 25 February 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Structure of Dark apoptosome in complex with Dronc CARD domain==
		+	<StructureSection load='3j9k' size='340' side='right' caption='[[3j9k]], [[Resolution\|resolution]] 4.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3j9k]] is a 32 chain structure with sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J9K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J9K FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3j9l\|3j9l]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j9k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j9k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3j9k RCSB], [http://www.ebi.ac.uk/pdbsum/3j9k PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ICENC_DROME ICENC_DROME]] Involved in the activation cascade of caspases responsible for apoptosis execution. Effector of steroid-mediated apoptosis during insect metamorphosis. Overexpression promotes programmed cell death. Interaction with th is required to suppress Nc-mediated cell death; via th-mediated ubiquitination of Nc. Rate-limiting caspase in rpr and W death pathway.<ref>PMID:10200258</ref> <ref>PMID:10675329</ref> <ref>PMID:10984473</ref> <ref>PMID:14517550</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Apoptosis is executed by a cascade of caspase activation. The autocatalytic activation of an initiator caspase, exemplified by caspase-9 in mammals or its ortholog, Dronc, in fruit flies, is facilitated by a multimeric adaptor complex known as the apoptosome. The underlying mechanism by which caspase-9 or Dronc is activated by the apoptosome remains unknown. Here we report the electron cryomicroscopic (cryo-EM) structure of the intact apoptosome from Drosophila melanogaster at 4.0 A resolution. Analysis of the Drosophila apoptosome, which comprises 16 molecules of the Dark protein (Apaf-1 ortholog), reveals molecular determinants that support the assembly of the 2.5-MDa complex. In the absence of dATP or ATP, Dronc zymogen potently induces formation of the Dark apoptosome, within which Dronc is efficiently activated. At 4.1 A resolution, the cryo-EM structure of the Dark apoptosome bound to the caspase recruitment domain (CARD) of Dronc (Dronc-CARD) reveals two stacked rings of Dronc-CARD that are sandwiched between two octameric rings of the Dark protein. The specific interactions between Dronc-CARD and both the CARD and the WD40 repeats of a nearby Dark protomer are indispensable for Dronc activation. These findings reveal important mechanistic insights into the activation of initiator caspase by the apoptosome.

-	~~The entry 3j9k is ON HOLD~~	+	Structure of the apoptosome: mechanistic insights into activation of an initiator caspase from Drosophila.,Pang Y, Bai XC, Yan C, Hao Q, Chen Z, Wang JW, Scheres SH, Shi Y Genes Dev. 2015 Feb 1;29(3):277-87. doi: 10.1101/gad.255877.114. PMID:25644603<ref>PMID:25644603</ref>

-	~~Authors: Pang~~, Y.~~, Bai, X., Yan, C., Hao, Q., Chen, Z., Wang, J., Scheres,~~ S.~~H.W., Shi, Y~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~Structure of Dark apoptosome in complex with Dronc CARD domain~~	+	== References ==
-	[[Category: ~~Unreleased Structures~~]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Drosophila melanogaster]]
		+	[[Category: Bai, X]]
		+	[[Category: Chen, Z]]
	[[Category: Hao, Q]]		[[Category: Hao, Q]]
-	[[Category: Bai, X]]
-	[[Category: Yan, C]]
	[[Category: Pang, Y]]		[[Category: Pang, Y]]
-	~~[[Category: Chen, Z]]~~	+	[[Category: Scheres, S H.W]]
-	[[Category: Scheres, S.H.W]]	+
	[[Category: Shi, Y]]		[[Category: Shi, Y]]
	[[Category: Wang, J]]		[[Category: Wang, J]]
		+	[[Category: Yan, C]]
		+	[[Category: Apoptosis]]
		+	[[Category: Caspase activation]]
		+	[[Category: Programmed cell death]]

OCA: Protected "3j9k" [edit=sysop:move=sysop]

OCA — Thu, 12 Feb 2015 06:52:53 GMT

Protected "3j9k" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:52, 12 February 2015

OCA: New page: '''Unreleased structure''' The entry 3j9k is ON HOLD Authors: Pang, Y., Bai, X., Yan, C., Hao, Q., Chen, Z., Wang, J., Scheres, S.H.W., Shi, Y. Description: Structure of Dark apoptosom...

OCA — Thu, 12 Feb 2015 06:52:51 GMT

New page: '''Unreleased structure''' The entry 3j9k is ON HOLD Authors: Pang, Y., Bai, X., Yan, C., Hao, Q., Chen, Z., Wang, J., Scheres, S.H.W., Shi, Y. Description: Structure of Dark apoptosom...

New page

'''Unreleased structure'''

The entry 3j9k is ON HOLD

Authors: Pang, Y., Bai, X., Yan, C., Hao, Q., Chen, Z., Wang, J., Scheres, S.H.W., Shi, Y.

Description: Structure of Dark apoptosome in complex with Dronc CARD domain
[[Category: Unreleased Structures]]
[[Category: Hao, Q]]
[[Category: Bai, X]]
[[Category: Yan, C]]
[[Category: Pang, Y]]
[[Category: Chen, Z]]
[[Category: Scheres, S.H.W]]
[[Category: Shi, Y]]
[[Category: Wang, J]]