3j9o - Revision history

OCA at 08:41, 7 February 2024

OCA — Wed, 07 Feb 2024 08:41:27 GMT

←Older revision		Revision as of 08:41, 7 February 2024
Line 3:		Line 3:
	<SX load='3j9o' size='340' side='right' viewer='molstar' caption='[[3j9o]], [[Resolution\|resolution]] 3.70Å' scene=''>		<SX load='3j9o' size='340' side='right' viewer='molstar' caption='[[3j9o]], [[Resolution\|resolution]] 3.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3j9o]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Francisella_tularensis_subsp.~~_novicida_u112~~ Francisella tularensis subsp. novicida ~~u112~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J9O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J9O FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3j9o]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Francisella_tularensis_subsp._novicida_U112 Francisella tularensis subsp. novicida U112]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J9O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J9O FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j9o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j9o OCA], [https://pdbe.org/3j9o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j9o RCSB], [https://www.ebi.ac.uk/pdbsum/3j9o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j9o ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.7Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j9o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j9o OCA], [https://pdbe.org/3j9o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j9o RCSB], [https://www.ebi.ac.uk/pdbsum/3j9o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j9o ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/A0Q7I5_FRATN A0Q7I5_FRATN]
-	Type VI secretion systems (T6SSs) are newly identified contractile nanomachines that translocate effector proteins across bacterial membranes. The Francisella pathogenicity island, required for bacterial phagosome escape, intracellular replication, and virulence, was presumed to encode a T6SS-like apparatus. Here, we experimentally confirm the identity of this T6SS and, by cryo electron microscopy (cryoEM), show the structure of its post-contraction sheath at 3.7 A resolution. We demonstrate the assembly of this T6SS by IglA/IglB and secretion of its putative effector proteins in response to environmental stimuli. The sheath has a quaternary structure with handedness opposite that of contracted sheath of T4 phage tail and is organized in an interlaced two-dimensional array by means of beta sheet augmentation. By structure-based mutagenesis, we show that this interlacing is essential to secretion, phagosomal escape, and intracellular replication. Our atomic model of the T6SS will facilitate design of drugs targeting this highly prevalent secretion apparatus.	+
-		+
-	~~Atomic Structure of T6SS Reveals Interlaced Array Essential to Function.,Clemens DL, Ge P, Lee BY, Horwitz MA, Zhou ZH Cell. 2015 Feb 26;160(5)~~:~~940-51. doi: 10.1016~~/~~j.cell.2015.02.005. PMID:25723168<ref>PMID:25723168</ref>~~	+
-		+
-	~~From MEDLINE®~~/~~PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 3j9o" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</SX>		</SX>
-	[[Category: Francisella tularensis subsp. novicida ~~u112~~]]	+	[[Category: Francisella tularensis subsp. novicida U112]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Clemens~~, D L~~]]	+	[[Category: Clemens DL]]
-	[[Category: Ge, P]]	+	[[Category: Ge P]]
-	[[Category: Horwitz~~, M A~~]]	+	[[Category: Horwitz MA]]
-	[[Category: Lee, B Y]]	+	[[Category: Lee B-Y]]
-	[[Category: Zhou~~, Z H]]~~	+	[[Category: Zhou ZH]]
-	~~[[Category: Structural protein]]~~	+
-	~~[[Category: T6ss~~]]	+

OCA at 13:29, 4 May 2022

OCA — Wed, 04 May 2022 13:29:02 GMT

←Older revision		Revision as of 13:29, 4 May 2022
Line 3:		Line 3:
	<SX load='3j9o' size='340' side='right' viewer='molstar' caption='[[3j9o]], [[Resolution\|resolution]] 3.70Å' scene=''>		<SX load='3j9o' size='340' side='right' viewer='molstar' caption='[[3j9o]], [[Resolution\|resolution]] 3.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3j9o]] is a 12 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Francisella_tularensis_subsp._novicida_u112 Francisella tularensis subsp. novicida u112]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J9O OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=3J9O FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3j9o]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Francisella_tularensis_subsp._novicida_u112 Francisella tularensis subsp. novicida u112]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J9O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J9O FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=3j9o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j9o OCA], [~~http~~://pdbe.org/3j9o PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3j9o RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3j9o PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3j9o ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j9o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j9o OCA], [https://pdbe.org/3j9o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j9o RCSB], [https://www.ebi.ac.uk/pdbsum/3j9o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j9o ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 18:15, 10 April 2020

OCA — Fri, 10 Apr 2020 18:15:05 GMT

←Older revision		Revision as of 18:15, 10 April 2020
Line 3:		Line 3:
	<SX load='3j9o' size='340' side='right' viewer='molstar' caption='[[3j9o]], [[Resolution\|resolution]] 3.70Å' scene=''>		<SX load='3j9o' size='340' side='right' viewer='molstar' caption='[[3j9o]], [[Resolution\|resolution]] 3.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3j9o]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Francisella_tularensis_subsp._novicida_u112 Francisella tularensis subsp. novicida u112]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J9O OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3J9O FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3j9o]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Francisella_tularensis_subsp._novicida_u112 Francisella tularensis subsp. novicida u112]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J9O OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3J9O FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3j9o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j9o OCA], [http://pdbe.org/3j9o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3j9o RCSB], [http://www.ebi.ac.uk/pdbsum/3j9o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3j9o ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3j9o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j9o OCA], [http://pdbe.org/3j9o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3j9o RCSB], [http://www.ebi.ac.uk/pdbsum/3j9o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3j9o ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 17:32, 6 March 2020

OCA — Fri, 06 Mar 2020 17:32:37 GMT

←Older revision		Revision as of 17:32, 6 March 2020
Line 1:		Line 1:

	==CryoEM structure of a type VI secretion system==		==CryoEM structure of a type VI secretion system==
-	<~~StructureSection~~ load='3j9o' size='340' side='right' caption='[[3j9o]], [[Resolution\|resolution]] 3.70Å' scene=''>	+	<SX load='3j9o' size='340' side='right' viewer='molstar' caption='[[3j9o]], [[Resolution\|resolution]] 3.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3j9o]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Francisella_tularensis_subsp._novicida_u112 Francisella tularensis subsp. novicida u112]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J9O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J9O FirstGlance]. <br>		<table><tr><td colspan='2'>[[3j9o]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Francisella_tularensis_subsp._novicida_u112 Francisella tularensis subsp. novicida u112]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J9O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J9O FirstGlance]. <br>
Line 18:		Line 18:
	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Francisella tularensis subsp. novicida u112]]		[[Category: Francisella tularensis subsp. novicida u112]]
		+	[[Category: Large Structures]]
	[[Category: Clemens, D L]]		[[Category: Clemens, D L]]
	[[Category: Ge, P]]		[[Category: Ge, P]]

OCA at 07:31, 23 December 2016

OCA — Fri, 23 Dec 2016 07:31:13 GMT

←Older revision		Revision as of 07:31, 23 December 2016
Line 1:		Line 1:
		+
	==CryoEM structure of a type VI secretion system==		==CryoEM structure of a type VI secretion system==
	<StructureSection load='3j9o' size='340' side='right' caption='[[3j9o]], [[Resolution\|resolution]] 3.70Å' scene=''>		<StructureSection load='3j9o' size='340' side='right' caption='[[3j9o]], [[Resolution\|resolution]] 3.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3j9o]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Francisella_tularensis_subsp._novicida_u112 Francisella tularensis subsp. novicida u112]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J9O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J9O FirstGlance]. <br>		<table><tr><td colspan='2'>[[3j9o]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Francisella_tularensis_subsp._novicida_u112 Francisella tularensis subsp. novicida u112]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J9O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J9O FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j9o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j9o OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3j9o RCSB], [http://www.ebi.ac.uk/pdbsum/3j9o PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j9o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j9o OCA], [http://pdbe.org/3j9o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3j9o RCSB], [http://www.ebi.ac.uk/pdbsum/3j9o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3j9o ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 13:		Line 14:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3j9o" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 12:07, 18 March 2015

OCA — Wed, 18 Mar 2015 12:07:59 GMT

←Older revision		Revision as of 12:07, 18 March 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==CryoEM structure of a type VI secretion system==
		+	<StructureSection load='3j9o' size='340' side='right' caption='[[3j9o]], [[Resolution\|resolution]] 3.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3j9o]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Francisella_tularensis_subsp._novicida_u112 Francisella tularensis subsp. novicida u112]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J9O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J9O FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j9o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j9o OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3j9o RCSB], [http://www.ebi.ac.uk/pdbsum/3j9o PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Type VI secretion systems (T6SSs) are newly identified contractile nanomachines that translocate effector proteins across bacterial membranes. The Francisella pathogenicity island, required for bacterial phagosome escape, intracellular replication, and virulence, was presumed to encode a T6SS-like apparatus. Here, we experimentally confirm the identity of this T6SS and, by cryo electron microscopy (cryoEM), show the structure of its post-contraction sheath at 3.7 A resolution. We demonstrate the assembly of this T6SS by IglA/IglB and secretion of its putative effector proteins in response to environmental stimuli. The sheath has a quaternary structure with handedness opposite that of contracted sheath of T4 phage tail and is organized in an interlaced two-dimensional array by means of beta sheet augmentation. By structure-based mutagenesis, we show that this interlacing is essential to secretion, phagosomal escape, and intracellular replication. Our atomic model of the T6SS will facilitate design of drugs targeting this highly prevalent secretion apparatus.

-	~~The entry 3j9o is ON HOLD~~	+	Atomic Structure of T6SS Reveals Interlaced Array Essential to Function.,Clemens DL, Ge P, Lee BY, Horwitz MA, Zhou ZH Cell. 2015 Feb 26;160(5):940-51. doi: 10.1016/j.cell.2015.02.005. PMID:25723168<ref>PMID:25723168</ref>

-	~~Authors: Clemens~~, D.L.~~, Ge, P., Lee, B.-Y., Horwitz, M.A., Zhou, Z.H~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description: CryoEM structure of a type VI secretion system~~	+	== References ==
-	~~[[Category: Unreleased Structures]]~~	+	<references/>
-	~~[[Category: Zhou, Z.H]]~~	+	__TOC__
-	[[Category: ~~Horwitz, M~~.A]]	+	</StructureSection>
-	[[Category: Clemens, D.L]]	+	[[Category: Francisella tularensis subsp. novicida u112]]
-	~~[[Category: Lee, B.-Y~~]]	+	[[Category: Clemens, D L]]
	[[Category: Ge, P]]		[[Category: Ge, P]]
		+	[[Category: Horwitz, M A]]
		+	[[Category: Lee, B Y]]
		+	[[Category: Zhou, Z H]]
		+	[[Category: Structural protein]]
		+	[[Category: T6ss]]

OCA: Protected "3j9o" [edit=sysop:move=sysop]

OCA — Wed, 18 Feb 2015 07:58:39 GMT

Protected "3j9o" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:58, 18 February 2015

OCA: New page: '''Unreleased structure''' The entry 3j9o is ON HOLD Authors: Clemens, D.L., Ge, P., Lee, B.-Y., Horwitz, M.A., Zhou, Z.H. Description: CryoEM structure of a type VI secretion system [...

OCA — Wed, 18 Feb 2015 07:58:38 GMT

New page: '''Unreleased structure''' The entry 3j9o is ON HOLD Authors: Clemens, D.L., Ge, P., Lee, B.-Y., Horwitz, M.A., Zhou, Z.H. Description: CryoEM structure of a type VI secretion system [...

New page

'''Unreleased structure'''

The entry 3j9o is ON HOLD

Authors: Clemens, D.L., Ge, P., Lee, B.-Y., Horwitz, M.A., Zhou, Z.H.

Description: CryoEM structure of a type VI secretion system
[[Category: Unreleased Structures]]
[[Category: Zhou, Z.H]]
[[Category: Horwitz, M.A]]
[[Category: Clemens, D.L]]
[[Category: Lee, B.-Y]]
[[Category: Ge, P]]