3jar - Revision history

OCA at 08:42, 7 February 2024

OCA — Wed, 07 Feb 2024 08:42:21 GMT

←Older revision		Revision as of 08:42, 7 February 2024
Line 3:		Line 3:
	<SX load='3jar' size='340' side='right' viewer='molstar' caption='[[3jar]], [[Resolution\|resolution]] 3.50Å' scene=''>		<SX load='3jar' size='340' side='right' viewer='molstar' caption='[[3jar]], [[Resolution\|resolution]] 3.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3jar]] is a 14 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~] and [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JAR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3JAR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3jar]] is a 14 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JAR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3JAR FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>~~, ~~<scene name='pdbligand=GTP:GUANOSINE-~~5~~-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.5Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[3jak\|3jak]], [[3jal\|3jal]], [[3jas\|3jas]], [[3jat\|3jat]]~~</~~div~~><~~/td~~></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<~~tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">MAPRE3 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3jar FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jar OCA], [https://pdbe.org/3jar PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3jar RCSB], [https://www.ebi.ac.uk/pdbsum/3jar PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3jar ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3jar FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jar OCA], [https://pdbe.org/3jar PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3jar RCSB], [https://www.ebi.ac.uk/pdbsum/3jar PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3jar ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/TBA1B_PIG TBA1B_PIG]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [[https://www.uniprot.org/uniprot/MARE3_HUMAN MARE3_HUMAN]] Binds to the plus end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes microtubule growth. May be involved in spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration (By similarity).<ref>PMID:19255245</ref> [[https://www.uniprot.org/uniprot/TBB_PIG TBB_PIG]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.	+	[https://www.uniprot.org/uniprot/TBA1B_PIG TBA1B_PIG] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Microtubule (MT) dynamic instability is driven by GTP hydrolysis and regulated by microtubule-associated proteins, including the plus-end tracking end-binding protein (EB) family. We report six cryo-electron microscopy (cryo-EM) structures of MTs, at 3.5 A or better resolution, bound to GMPCPP, GTPgammaS, or GDP, either decorated with kinesin motor domain after polymerization or copolymerized with EB3. Subtle changes around the E-site nucleotide during hydrolysis trigger conformational changes in alpha-tubulin around an "anchor point," leading to global lattice rearrangements and strain generation. Unlike the extended lattice of the GMPCPP-MT, the EB3-bound GTPgammaS-MT has a compacted lattice that differs in lattice twist from that of the also compacted GDP-MT. These results and the observation that EB3 promotes rapid hydrolysis of GMPCPP suggest that EB proteins modulate structural transitions at growing MT ends by recognizing and promoting an intermediate state generated during GTP hydrolysis. Our findings explain both EBs end-tracking behavior and their effect on microtubule dynamics.	+
-		+
-	Mechanistic Origin of Microtubule Dynamic Instability and Its Modulation by EB Proteins.,Zhang R, Alushin GM, Brown A, Nogales E Cell. 2015 Jul 28. pii: S0092-8674(15)00849-1. doi: 10.1016/j.cell.2015.07.012. PMID:26234155<ref>PMID:26234155</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3jar" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</SX>		</SX>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Sus scrofa]]		[[Category: Sus scrofa]]
-	[[Category: Nogales, E]]	+	[[Category: Nogales E]]
-	[[Category: Zhang, R]]	+	[[Category: Zhang R]]
-	~~[[Category: Eb3]]~~	+
-	~~[[Category: Gdp]]~~	+
-	~~[[Category: Microtubule]]~~	+
-	~~[[Category: Structural protein~~]]	+

OCA at 13:30, 4 May 2022

OCA — Wed, 04 May 2022 13:30:12 GMT

←Older revision		Revision as of 13:30, 4 May 2022
Line 3:		Line 3:
	<SX load='3jar' size='340' side='right' viewer='molstar' caption='[[3jar]], [[Resolution\|resolution]] 3.50Å' scene=''>		<SX load='3jar' size='340' side='right' viewer='molstar' caption='[[3jar]], [[Resolution\|resolution]] 3.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3jar]] is a 14 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human] and [~~http~~://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JAR OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=3JAR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3jar]] is a 14 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human] and [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JAR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3JAR FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3jak\|3jak]], [[3jal\|3jal]], [[3jas\|3jas]], [[3jat\|3jat]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3jak\|3jak]], [[3jal\|3jal]], [[3jas\|3jas]], [[3jat\|3jat]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MAPRE3 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MAPRE3 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=3jar FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jar OCA], [~~http~~://pdbe.org/3jar PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3jar RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3jar PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3jar ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3jar FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jar OCA], [https://pdbe.org/3jar PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3jar RCSB], [https://www.ebi.ac.uk/pdbsum/3jar PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3jar ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/TBA1B_PIG TBA1B_PIG]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [[~~http~~://www.uniprot.org/uniprot/MARE3_HUMAN MARE3_HUMAN]] Binds to the plus end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes microtubule growth. May be involved in spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration (By similarity).<ref>PMID:19255245</ref> [[~~http~~://www.uniprot.org/uniprot/TBB_PIG TBB_PIG]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.	+	[[https://www.uniprot.org/uniprot/TBA1B_PIG TBA1B_PIG]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [[https://www.uniprot.org/uniprot/MARE3_HUMAN MARE3_HUMAN]] Binds to the plus end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes microtubule growth. May be involved in spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration (By similarity).<ref>PMID:19255245</ref> [[https://www.uniprot.org/uniprot/TBB_PIG TBB_PIG]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 18:22, 10 April 2020

OCA — Fri, 10 Apr 2020 18:22:01 GMT

←Older revision		Revision as of 18:22, 10 April 2020
Line 3:		Line 3:
	<SX load='3jar' size='340' side='right' viewer='molstar' caption='[[3jar]], [[Resolution\|resolution]] 3.50Å' scene=''>		<SX load='3jar' size='340' side='right' viewer='molstar' caption='[[3jar]], [[Resolution\|resolution]] 3.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3jar]] is a 14 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JAR OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3JAR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3jar]] is a 14 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JAR OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3JAR FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3jak\|3jak]], [[3jal\|3jal]], [[3jas\|3jas]], [[3jat\|3jat]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3jak\|3jak]], [[3jal\|3jal]], [[3jas\|3jas]], [[3jat\|3jat]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MAPRE3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MAPRE3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3jar FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jar OCA], [http://pdbe.org/3jar PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3jar RCSB], [http://www.ebi.ac.uk/pdbsum/3jar PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3jar ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3jar FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jar OCA], [http://pdbe.org/3jar PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3jar RCSB], [http://www.ebi.ac.uk/pdbsum/3jar PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3jar ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 17:35, 6 March 2020

OCA — Fri, 06 Mar 2020 17:35:39 GMT

←Older revision		Revision as of 17:35, 6 March 2020
Line 1:		Line 1:

	==Cryo-EM structure of GDP-microtubule co-polymerized with EB3==		==Cryo-EM structure of GDP-microtubule co-polymerized with EB3==
-	<~~StructureSection~~ load='3jar' size='340' side='right'caption='[[3jar]], [[Resolution\|resolution]] 3.50Å' scene=''>	+	<SX load='3jar' size='340' side='right' viewer='molstar' caption='[[3jar]], [[Resolution\|resolution]] 3.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3jar]] is a 14 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JAR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3JAR FirstGlance]. <br>		<table><tr><td colspan='2'>[[3jar]] is a 14 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JAR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3JAR FirstGlance]. <br>
Line 23:		Line 23:
	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Human]]		[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]

OCA at 08:11, 18 December 2019

OCA — Wed, 18 Dec 2019 08:11:40 GMT

←Older revision		Revision as of 08:11, 18 December 2019
Line 1:		Line 1:

	==Cryo-EM structure of GDP-microtubule co-polymerized with EB3==		==Cryo-EM structure of GDP-microtubule co-polymerized with EB3==
-	<StructureSection load='3jar' size='340' side='right' caption='[[3jar]], [[Resolution\|resolution]] 3.50Å' scene=''>	+	<StructureSection load='3jar' size='340' side='right'caption='[[3jar]], [[Resolution\|resolution]] 3.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3jar]] is a 14 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JAR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3JAR FirstGlance]. <br>		<table><tr><td colspan='2'>[[3jar]] is a 14 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JAR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3JAR FirstGlance]. <br>
Line 25:		Line 25:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Sus scrofa]]		[[Category: Sus scrofa]]
	[[Category: Nogales, E]]		[[Category: Nogales, E]]

OCA at 08:23, 18 July 2018

OCA — Wed, 18 Jul 2018 08:23:53 GMT

←Older revision		Revision as of 08:23, 18 July 2018
Line 1:		Line 1:
		+
	==Cryo-EM structure of GDP-microtubule co-polymerized with EB3==		==Cryo-EM structure of GDP-microtubule co-polymerized with EB3==
	<StructureSection load='3jar' size='340' side='right' caption='[[3jar]], [[Resolution\|resolution]] 3.50Å' scene=''>		<StructureSection load='3jar' size='340' side='right' caption='[[3jar]], [[Resolution\|resolution]] 3.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3jar]] is a 14 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JAR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3JAR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3jar]] is a 14 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JAR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3JAR FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3jak\|3jak]], [[3jal\|3jal]], [[3jas\|3jas]], [[3jat\|3jat]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3jak\|3jak]], [[3jal\|3jal]], [[3jas\|3jas]], [[3jat\|3jat]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3jar FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jar OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3jar RCSB], [http://www.ebi.ac.uk/pdbsum/3jar PDBsum]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MAPRE3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3jar FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jar OCA], [http://pdbe.org/3jar PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3jar RCSB], [http://www.ebi.ac.uk/pdbsum/3jar PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3jar ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3jar" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Sus scrofa]]		[[Category: Sus scrofa]]
	[[Category: Nogales, E]]		[[Category: Nogales, E]]

OCA at 14:38, 12 August 2015

OCA — Wed, 12 Aug 2015 14:38:57 GMT

←Older revision		Revision as of 14:38, 12 August 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Cryo-EM structure of GDP-microtubule co-polymerized with EB3==
		+	<StructureSection load='3jar' size='340' side='right' caption='[[3jar]], [[Resolution\|resolution]] 3.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3jar]] is a 14 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JAR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3JAR FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3jak\|3jak]], [[3jal\|3jal]], [[3jas\|3jas]], [[3jat\|3jat]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3jar FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jar OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3jar RCSB], [http://www.ebi.ac.uk/pdbsum/3jar PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/TBA1B_PIG TBA1B_PIG]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [[http://www.uniprot.org/uniprot/MARE3_HUMAN MARE3_HUMAN]] Binds to the plus end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes microtubule growth. May be involved in spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration (By similarity).<ref>PMID:19255245</ref> [[http://www.uniprot.org/uniprot/TBB_PIG TBB_PIG]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Microtubule (MT) dynamic instability is driven by GTP hydrolysis and regulated by microtubule-associated proteins, including the plus-end tracking end-binding protein (EB) family. We report six cryo-electron microscopy (cryo-EM) structures of MTs, at 3.5 A or better resolution, bound to GMPCPP, GTPgammaS, or GDP, either decorated with kinesin motor domain after polymerization or copolymerized with EB3. Subtle changes around the E-site nucleotide during hydrolysis trigger conformational changes in alpha-tubulin around an "anchor point," leading to global lattice rearrangements and strain generation. Unlike the extended lattice of the GMPCPP-MT, the EB3-bound GTPgammaS-MT has a compacted lattice that differs in lattice twist from that of the also compacted GDP-MT. These results and the observation that EB3 promotes rapid hydrolysis of GMPCPP suggest that EB proteins modulate structural transitions at growing MT ends by recognizing and promoting an intermediate state generated during GTP hydrolysis. Our findings explain both EBs end-tracking behavior and their effect on microtubule dynamics.

-	~~The entry 3jar is ON HOLD~~	+	Mechanistic Origin of Microtubule Dynamic Instability and Its Modulation by EB Proteins.,Zhang R, Alushin GM, Brown A, Nogales E Cell. 2015 Jul 28. pii: S0092-8674(15)00849-1. doi: 10.1016/j.cell.2015.07.012. PMID:26234155<ref>PMID:26234155</ref>

-	~~Authors: Zhang~~, R.~~, Nogales, E~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description: Cryo-EM structure of GDP-microtubule co-polymerized with EB3~~	+	== References ==
-	[[Category: ~~Unreleased Structures~~]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Sus scrofa]]
	[[Category: Nogales, E]]		[[Category: Nogales, E]]
	[[Category: Zhang, R]]		[[Category: Zhang, R]]
		+	[[Category: Eb3]]
		+	[[Category: Gdp]]
		+	[[Category: Microtubule]]
		+	[[Category: Structural protein]]

OCA: Protected "3jar" [edit=sysop:move=sysop]

OCA — Wed, 01 Jul 2015 06:50:01 GMT

Protected "3jar" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:50, 1 July 2015

OCA: New page: '''Unreleased structure''' The entry 3jar is ON HOLD Authors: Zhang, R., Nogales, E. Description: Cryo-EM structure of GDP-microtubule co-polymerized with EB3 [[Category: Unreleased St...

OCA — Wed, 01 Jul 2015 06:50:00 GMT

New page: '''Unreleased structure''' The entry 3jar is ON HOLD Authors: Zhang, R., Nogales, E. Description: Cryo-EM structure of GDP-microtubule co-polymerized with EB3 [[Category: Unreleased St...

New page

'''Unreleased structure'''

The entry 3jar is ON HOLD

Authors: Zhang, R., Nogales, E.

Description: Cryo-EM structure of GDP-microtubule co-polymerized with EB3
[[Category: Unreleased Structures]]
[[Category: Nogales, E]]
[[Category: Zhang, R]]