3jas - Revision history

OCA at 08:42, 7 February 2024

OCA — Wed, 07 Feb 2024 08:42:23 GMT

←Older revision		Revision as of 08:42, 7 February 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3jas]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JAS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3JAS FirstGlance]. <br>		<table><tr><td colspan='2'>[[3jas]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JAS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3JAS FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.5Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3jak\|3jak]], [[3jal\|3jal]], [[3jar\|3jar]], [[3jat\|3jat]]</div></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3jas FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jas OCA], [https://pdbe.org/3jas PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3jas RCSB], [https://www.ebi.ac.uk/pdbsum/3jas PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3jas ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3jas FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jas OCA], [https://pdbe.org/3jas PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3jas RCSB], [https://www.ebi.ac.uk/pdbsum/3jas PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3jas ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/TBA1B_PIG TBA1B_PIG]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [[https://www.uniprot.org/uniprot/TBB_PIG TBB_PIG]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.	+	[https://www.uniprot.org/uniprot/TBA1B_PIG TBA1B_PIG] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Microtubule (MT) dynamic instability is driven by GTP hydrolysis and regulated by microtubule-associated proteins, including the plus-end tracking end-binding protein (EB) family. We report six cryo-electron microscopy (cryo-EM) structures of MTs, at 3.5 A or better resolution, bound to GMPCPP, GTPgammaS, or GDP, either decorated with kinesin motor domain after polymerization or copolymerized with EB3. Subtle changes around the E-site nucleotide during hydrolysis trigger conformational changes in alpha-tubulin around an "anchor point," leading to global lattice rearrangements and strain generation. Unlike the extended lattice of the GMPCPP-MT, the EB3-bound GTPgammaS-MT has a compacted lattice that differs in lattice twist from that of the also compacted GDP-MT. These results and the observation that EB3 promotes rapid hydrolysis of GMPCPP suggest that EB proteins modulate structural transitions at growing MT ends by recognizing and promoting an intermediate state generated during GTP hydrolysis. Our findings explain both EBs end-tracking behavior and their effect on microtubule dynamics.	+
-		+
-	Mechanistic Origin of Microtubule Dynamic Instability and Its Modulation by EB Proteins.,Zhang R, Alushin GM, Brown A, Nogales E Cell. 2015 Jul 28. pii: S0092-8674(15)00849-1. doi: 10.1016/j.cell.2015.07.012. PMID:26234155<ref>PMID:26234155</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3jas" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</SX>		</SX>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Sus scrofa]]		[[Category: Sus scrofa]]
-	[[Category: Nogales, E]]	+	[[Category: Nogales E]]
-	[[Category: Zhang, R]]	+	[[Category: Zhang R]]
-	~~[[Category: Gdp]]~~	+
-	~~[[Category: Kinesin]]~~	+
-	~~[[Category: Microtubule]]~~	+
-	~~[[Category: Structural protein~~]]	+

OCA at 13:30, 4 May 2022

OCA — Wed, 04 May 2022 13:30:14 GMT

←Older revision		Revision as of 13:30, 4 May 2022
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	<SX load='3jas' size='340' side='right' viewer='molstar' caption='[[3jas]], [[Resolution\|resolution]] 3.50Å' scene=''>		<SX load='3jas' size='340' side='right' viewer='molstar' caption='[[3jas]], [[Resolution\|resolution]] 3.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3jas]] is a 12 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JAS OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=3JAS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3jas]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JAS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3JAS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3jak\|3jak]], [[3jal\|3jal]], [[3jar\|3jar]], [[3jat\|3jat]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3jak\|3jak]], [[3jal\|3jal]], [[3jar\|3jar]], [[3jat\|3jat]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=3jas FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jas OCA], [~~http~~://pdbe.org/3jas PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3jas RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3jas PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3jas ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3jas FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jas OCA], [https://pdbe.org/3jas PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3jas RCSB], [https://www.ebi.ac.uk/pdbsum/3jas PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3jas ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/TBA1B_PIG TBA1B_PIG]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [[~~http~~://www.uniprot.org/uniprot/TBB_PIG TBB_PIG]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.	+	[[https://www.uniprot.org/uniprot/TBA1B_PIG TBA1B_PIG]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [[https://www.uniprot.org/uniprot/TBB_PIG TBB_PIG]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 18:22, 10 April 2020

OCA — Fri, 10 Apr 2020 18:22:11 GMT

←Older revision		Revision as of 18:22, 10 April 2020
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	<SX load='3jas' size='340' side='right' viewer='molstar' caption='[[3jas]], [[Resolution\|resolution]] 3.50Å' scene=''>		<SX load='3jas' size='340' side='right' viewer='molstar' caption='[[3jas]], [[Resolution\|resolution]] 3.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3jas]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JAS OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3JAS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3jas]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JAS OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3JAS FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3jak\|3jak]], [[3jal\|3jal]], [[3jar\|3jar]], [[3jat\|3jat]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3jak\|3jak]], [[3jal\|3jal]], [[3jar\|3jar]], [[3jat\|3jat]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3jas FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jas OCA], [http://pdbe.org/3jas PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3jas RCSB], [http://www.ebi.ac.uk/pdbsum/3jas PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3jas ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3jas FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jas OCA], [http://pdbe.org/3jas PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3jas RCSB], [http://www.ebi.ac.uk/pdbsum/3jas PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3jas ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 17:35, 6 March 2020

OCA — Fri, 06 Mar 2020 17:35:43 GMT

←Older revision		Revision as of 17:35, 6 March 2020
Line 1:		Line 1:

	==Cryo-EM structure of dynamic GDP-microtubule (14 protofilaments) decorated with kinesin==		==Cryo-EM structure of dynamic GDP-microtubule (14 protofilaments) decorated with kinesin==
-	<~~StructureSection~~ load='3jas' size='340' side='right'caption='[[3jas]], [[Resolution\|resolution]] 3.50Å' scene=''>	+	<SX load='3jas' size='340' side='right' viewer='molstar' caption='[[3jas]], [[Resolution\|resolution]] 3.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3jas]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JAS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3JAS FirstGlance]. <br>		<table><tr><td colspan='2'>[[3jas]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JAS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3JAS FirstGlance]. <br>
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	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Sus scrofa]]		[[Category: Sus scrofa]]

OCA at 08:11, 18 December 2019

OCA — Wed, 18 Dec 2019 08:11:39 GMT

←Older revision		Revision as of 08:11, 18 December 2019
Line 1:		Line 1:

	==Cryo-EM structure of dynamic GDP-microtubule (14 protofilaments) decorated with kinesin==		==Cryo-EM structure of dynamic GDP-microtubule (14 protofilaments) decorated with kinesin==
-	<StructureSection load='3jas' size='340' side='right' caption='[[3jas]], [[Resolution\|resolution]] 3.50Å' scene=''>	+	<StructureSection load='3jas' size='340' side='right'caption='[[3jas]], [[Resolution\|resolution]] 3.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3jas]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JAS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3JAS FirstGlance]. <br>		<table><tr><td colspan='2'>[[3jas]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JAS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3JAS FirstGlance]. <br>
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Sus scrofa]]		[[Category: Sus scrofa]]
	[[Category: Nogales, E]]		[[Category: Nogales, E]]

OCA at 08:24, 18 July 2018

OCA — Wed, 18 Jul 2018 08:24:05 GMT

←Older revision		Revision as of 08:24, 18 July 2018
Line 1:		Line 1:
		+
	==Cryo-EM structure of dynamic GDP-microtubule (14 protofilaments) decorated with kinesin==		==Cryo-EM structure of dynamic GDP-microtubule (14 protofilaments) decorated with kinesin==
	<StructureSection load='3jas' size='340' side='right' caption='[[3jas]], [[Resolution\|resolution]] 3.50Å' scene=''>		<StructureSection load='3jas' size='340' side='right' caption='[[3jas]], [[Resolution\|resolution]] 3.50Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3jak\|3jak]], [[3jal\|3jal]], [[3jar\|3jar]], [[3jat\|3jat]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3jak\|3jak]], [[3jal\|3jal]], [[3jar\|3jar]], [[3jat\|3jat]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3jas FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jas OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3jas RCSB], [http://www.ebi.ac.uk/pdbsum/3jas PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3jas FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jas OCA], [http://pdbe.org/3jas PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3jas RCSB], [http://www.ebi.ac.uk/pdbsum/3jas PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3jas ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3jas" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 14:30, 12 August 2015

OCA — Wed, 12 Aug 2015 14:30:30 GMT

←Older revision		Revision as of 14:30, 12 August 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Cryo-EM structure of dynamic GDP-microtubule (14 protofilaments) decorated with kinesin==
		+	<StructureSection load='3jas' size='340' side='right' caption='[[3jas]], [[Resolution\|resolution]] 3.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3jas]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JAS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3JAS FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3jak\|3jak]], [[3jal\|3jal]], [[3jar\|3jar]], [[3jat\|3jat]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3jas FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jas OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3jas RCSB], [http://www.ebi.ac.uk/pdbsum/3jas PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/TBA1B_PIG TBA1B_PIG]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [[http://www.uniprot.org/uniprot/TBB_PIG TBB_PIG]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Microtubule (MT) dynamic instability is driven by GTP hydrolysis and regulated by microtubule-associated proteins, including the plus-end tracking end-binding protein (EB) family. We report six cryo-electron microscopy (cryo-EM) structures of MTs, at 3.5 A or better resolution, bound to GMPCPP, GTPgammaS, or GDP, either decorated with kinesin motor domain after polymerization or copolymerized with EB3. Subtle changes around the E-site nucleotide during hydrolysis trigger conformational changes in alpha-tubulin around an "anchor point," leading to global lattice rearrangements and strain generation. Unlike the extended lattice of the GMPCPP-MT, the EB3-bound GTPgammaS-MT has a compacted lattice that differs in lattice twist from that of the also compacted GDP-MT. These results and the observation that EB3 promotes rapid hydrolysis of GMPCPP suggest that EB proteins modulate structural transitions at growing MT ends by recognizing and promoting an intermediate state generated during GTP hydrolysis. Our findings explain both EBs end-tracking behavior and their effect on microtubule dynamics.

-	~~The entry 3jas is ON HOLD~~	+	Mechanistic Origin of Microtubule Dynamic Instability and Its Modulation by EB Proteins.,Zhang R, Alushin GM, Brown A, Nogales E Cell. 2015 Jul 28. pii: S0092-8674(15)00849-1. doi: 10.1016/j.cell.2015.07.012. PMID:26234155<ref>PMID:26234155</ref>

-	~~Authors: Zhang~~, R.~~, Nogales, E~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description: Cryo-EM structure of dynamic GDP-microtubule (14 protofilaments) decorated with kinesin~~	+	== References ==
-	[[Category: ~~Unreleased Structures~~]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Sus scrofa]]
	[[Category: Nogales, E]]		[[Category: Nogales, E]]
	[[Category: Zhang, R]]		[[Category: Zhang, R]]
		+	[[Category: Gdp]]
		+	[[Category: Kinesin]]
		+	[[Category: Microtubule]]
		+	[[Category: Structural protein]]

OCA: Protected "3jas" [edit=sysop:move=sysop]

OCA — Wed, 01 Jul 2015 06:50:03 GMT

Protected "3jas" [edit=sysop:move=sysop]

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Revision as of 06:50, 1 July 2015

OCA: New page: '''Unreleased structure''' The entry 3jas is ON HOLD Authors: Zhang, R., Nogales, E. Description: Cryo-EM structure of dynamic GDP-microtubule (14 protofilaments) decorated with kinesi...

OCA — Wed, 01 Jul 2015 06:50:02 GMT

New page: '''Unreleased structure''' The entry 3jas is ON HOLD Authors: Zhang, R., Nogales, E. Description: Cryo-EM structure of dynamic GDP-microtubule (14 protofilaments) decorated with kinesi...

New page

'''Unreleased structure'''

The entry 3jas is ON HOLD

Authors: Zhang, R., Nogales, E.

Description: Cryo-EM structure of dynamic GDP-microtubule (14 protofilaments) decorated with kinesin
[[Category: Unreleased Structures]]
[[Category: Nogales, E]]
[[Category: Zhang, R]]