3jqr - Revision history

OCA at 16:04, 1 November 2023

OCA — Wed, 01 Nov 2023 16:04:51 GMT

←Older revision		Revision as of 16:04, 1 November 2023
Line 3:		Line 3:
	<StructureSection load='3jqr' size='340' side='right'caption='[[3jqr]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='3jqr' size='340' side='right'caption='[[3jqr]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3jqr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Plafa Plafa~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JQR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3JQR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3jqr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JQR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3JQR FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=FAD:FLAVIN~~-~~ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>~~[[~~Related_structure~~\|~~Related:~~]]~~</b></td><td class="sblockDat"><div style='overflow: auto~~; ~~max-height: 3em;'>[[3jqp\|3jqp]], [[3jqq\|3jqq]]</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>PfFNR, PFF1115w ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id~~=5833 PLAFA])</td></tr>~~	+
-	~~<tr id='activity'><td class~~="~~sblockLbl~~"><~~b>Activity:</b></td><td class~~=~~"sblockDat"><span class~~=~~'plainlinks'>[https~~:~~//en.wikipedia.org/wiki/Ferredoxin~~-~~-NADP(~~+~~)_reductase Ferredoxin--NADP(+) reductase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.18.1.2 1.18.1.2]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3jqr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jqr OCA], [https://pdbe.org/3jqr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3jqr RCSB], [https://www.ebi.ac.uk/pdbsum/3jqr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3jqr ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3jqr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jqr OCA], [https://pdbe.org/3jqr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3jqr RCSB], [https://www.ebi.ac.uk/pdbsum/3jqr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3jqr ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/FENR_PLAF7 FENR_PLAF7] May play a role in the terminal step of the DOXP/MEP pathway for isoprenoid precursor biosynthesis.<ref>PMID:16289098</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Plafa~~]]	+	[[Category: Plasmodium falciparum]]
-	[[Category: Bolognesi, M]]	+	[[Category: Bolognesi M]]
-	[[Category: Canevari, G]]	+	[[Category: Canevari G]]
-	[[Category: Milani, M]]	+	[[Category: Milani M]]
-	~~[[Category: Fad]]~~	+
-	~~[[Category: Ferredoxin-nadp+ reductase]]~~	+
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 07:48, 10 November 2021

OCA — Wed, 10 Nov 2021 07:48:45 GMT

←Older revision		Revision as of 07:48, 10 November 2021
Line 1:		Line 1:

	==Crystal structure of the H286L mutant of Ferredoxin-NADP+ reductase from Plasmodium falciparum==		==Crystal structure of the H286L mutant of Ferredoxin-NADP+ reductase from Plasmodium falciparum==
-	<StructureSection load='3jqr' size='340' side='right' caption='[[3jqr]], [[Resolution\|resolution]] 2.30Å' scene=''>	+	<StructureSection load='3jqr' size='340' side='right'caption='[[3jqr]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3jqr]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Plafa Plafa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JQR OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3JQR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3jqr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Plafa Plafa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JQR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3JQR FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3jqp\|3jqp]], [[3jqq\|3jqq]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3jqp\|3jqp]], [[3jqq\|3jqq]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PfFNR, PFF1115w ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5833 PLAFA])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PfFNR, PFF1115w ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5833 PLAFA])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Ferredoxin--NADP(+)_reductase Ferredoxin--NADP(+) reductase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.18.1.2 1.18.1.2] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Ferredoxin--NADP(+)_reductase Ferredoxin--NADP(+) reductase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.18.1.2 1.18.1.2] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3jqr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jqr OCA], [~~http~~://pdbe.org/3jqr PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3jqr RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3jqr PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3jqr ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3jqr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jqr OCA], [https://pdbe.org/3jqr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3jqr RCSB], [https://www.ebi.ac.uk/pdbsum/3jqr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3jqr ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Plafa]]		[[Category: Plafa]]
	[[Category: Bolognesi, M]]		[[Category: Bolognesi, M]]

OCA at 09:03, 26 December 2018

OCA — Wed, 26 Dec 2018 09:03:19 GMT

←Older revision		Revision as of 09:03, 26 December 2018
Line 14:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jq/3jqr_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jq/3jqr_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 11:57, 5 August 2016

OCA — Fri, 05 Aug 2016 11:57:52 GMT

←Older revision		Revision as of 11:57, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of the H286L mutant of Ferredoxin-NADP+ reductase from Plasmodium falciparum==		==Crystal structure of the H286L mutant of Ferredoxin-NADP+ reductase from Plasmodium falciparum==
	<StructureSection load='3jqr' size='340' side='right' caption='[[3jqr]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='3jqr' size='340' side='right' caption='[[3jqr]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3jqr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Plasmodium_falciparum Plasmodium falciparum~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JQR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3JQR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3jqr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Plafa Plafa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JQR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3JQR FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3jqp\|3jqp]], [[3jqq\|3jqq]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3jqp\|3jqp]], [[3jqq\|3jqq]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PfFNR, PFF1115w ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5833 ~~Plasmodium falciparum~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PfFNR, PFF1115w ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5833 PLAFA])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ferredoxin--NADP(+)_reductase Ferredoxin--NADP(+) reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.18.1.2 1.18.1.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ferredoxin--NADP(+)_reductase Ferredoxin--NADP(+) reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.18.1.2 1.18.1.2] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3jqr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jqr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3jqr RCSB], [http://www.ebi.ac.uk/pdbsum/3jqr PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3jqr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jqr OCA], [http://pdbe.org/3jqr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3jqr RCSB], [http://www.ebi.ac.uk/pdbsum/3jqr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3jqr ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 17:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3jqr ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 27:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3jqr" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Plasmodium falciparum~~]]	+	[[Category: Plafa]]
	[[Category: Bolognesi, M]]		[[Category: Bolognesi, M]]
	[[Category: Canevari, G]]		[[Category: Canevari, G]]

OCA at 17:28, 18 December 2014

OCA — Thu, 18 Dec 2014 17:28:58 GMT

←Older revision		Revision as of 17:28, 18 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3jqr\| PDB=3jqr \| SCENE= }}~~	+	==Crystal structure of the H286L mutant of Ferredoxin-NADP+ reductase from Plasmodium falciparum==
-	===Crystal structure of the H286L mutant of Ferredoxin-NADP+ reductase from Plasmodium falciparum===	+	<StructureSection load='3jqr' size='340' side='right' caption='[[3jqr]], [[Resolution\|resolution]] 2.30Å' scene=''>
-	~~{{ABSTRACT_PUBMED_19736991}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3jqr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JQR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3JQR FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3jqp\|3jqp]], [[3jqq\|3jqq]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PfFNR, PFF1115w ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5833 Plasmodium falciparum])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ferredoxin--NADP(+)_reductase Ferredoxin--NADP(+) reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.18.1.2 1.18.1.2] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3jqr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jqr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3jqr RCSB], [http://www.ebi.ac.uk/pdbsum/3jqr PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jq/3jqr_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The NADP-binding site of Plasmodium falciparum ferredoxin-NADP(+) reductase contains two basic residues, His286 and Lys249, conserved within the Plasmodium genus, but not in other plant-type homologues. Previous crystal studies indicated that His286 interacts with the adenine ring and with the 5'-phosphate of 2'-P-AMP, a ligand that mimics the adenylate moiety of NADP(H). Here we show that replacement of His286 with aliphatic residues results both in a decrease in the affinity of the enzyme for NADPH and in a decrease in k(cat), due to a lowered hydride-transfer rate. Unexpectedly, the mutation to Gln produces an enzyme more active than the wild-type one, whereas the change to Lys destabilizes the nicotinamide-isoalloxazine interaction, decreasing k(cat). On the basis of the crystal structure of selected mutants complexed with 2'-P-AMP, we conclude that the His286 side chain plays a dual role in catalysis both by providing binding energy for NADPH and by favoring the catalytically competent orientation of its nicotinamide ring. For the latter function, the H-bonding potential rather than the positively charged state of the His286 imidazole seems sufficient. Furthermore, we show that the Lys249Ala mutation decreases K(m)(NADPH) and K(d) for NADP(+) or 2'-P-AMP by a factor of 10. We propose that the Lys249 side chain participates in substrate recognition by interacting with the 2'-phosphate of NADP(H) and that this interaction was not observed in the crystal form of the enzyme-2'-P-AMP complex due to a conformational perturbation of the substrate-binding loop induced by dimerization.

-	~~==About this Structure==~~	+	Plasmodium falciparum ferredoxin-NADP+ reductase His286 plays a dual role in NADP(H) binding and catalysis.,Crobu D, Canevari G, Milani M, Pandini V, Vanoni MA, Bolognesi M, Zanetti G, Aliverti A Biochemistry. 2009 Oct 13;48(40):9525-33. PMID:19736991<ref>PMID:19736991</ref>
-	~~[[3jqr]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Plasmodium_falciparum Plasmodium falciparum]~~. ~~Full crystallographic information is available from [http~~:/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3JQR OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:019736991</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Plasmodium falciparum]]		[[Category: Plasmodium falciparum]]
-	[[Category: Bolognesi, M.]]	+	[[Category: Bolognesi, M]]
-	[[Category: Canevari, G.]]	+	[[Category: Canevari, G]]
-	[[Category: Milani, M.]]	+	[[Category: Milani, M]]
	[[Category: Fad]]		[[Category: Fad]]
	[[Category: Ferredoxin-nadp+ reductase]]		[[Category: Ferredoxin-nadp+ reductase]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]

OCA: Protected "3jqr" [edit=sysop:move=sysop]

OCA — Wed, 27 Mar 2013 09:22:44 GMT

Protected "3jqr" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:22, 27 March 2013

OCA at 09:22, 27 March 2013

OCA — Wed, 27 Mar 2013 09:22:40 GMT

←Older revision		Revision as of 09:22, 27 March 2013
Line 1:		Line 1:
-	{{Seed}}
-	[[Image:3jqr.jpg\|left\|200px]]
-
-	<!--
-	The line below this paragraph, containing "STRUCTURE_3jqr", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3jqr\| PDB=3jqr \| SCENE= }}		{{STRUCTURE_3jqr\| PDB=3jqr \| SCENE= }}
-
	===Crystal structure of the H286L mutant of Ferredoxin-NADP+ reductase from Plasmodium falciparum===		===Crystal structure of the H286L mutant of Ferredoxin-NADP+ reductase from Plasmodium falciparum===
-
-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_19736991}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 19736991 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_19736991}}		{{ABSTRACT_PUBMED_19736991}}

	==About this Structure==		==About this Structure==
-	~~3JQR~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JQR OCA].	+	[[3jqr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JQR OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~19736991~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019736991</ref><references group="xtra"/><references/>
	[[Category: Plasmodium falciparum]]		[[Category: Plasmodium falciparum]]
	[[Category: Bolognesi, M.]]		[[Category: Bolognesi, M.]]
Line 31:		Line 15:
	[[Category: Ferredoxin-nadp+ reductase]]		[[Category: Ferredoxin-nadp+ reductase]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 13 14:10:15 2010''

OCA at 12:10, 13 January 2010

OCA — Wed, 13 Jan 2010 12:10:16 GMT

←Older revision		Revision as of 12:10, 13 January 2010
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3jqr.jpg\|left\|200px]]

-	The ~~entry 3jqr~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3jqr", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3jqr\| PDB=3jqr \| SCENE= }}

-	~~Authors: Canevari, G., Milani, M., Bolognesi, M.~~	+	===Crystal structure of the H286L mutant of Ferredoxin-NADP+ reductase from Plasmodium falciparum===

-	Description: Crystal structure of the H286L mutant of Ferredoxin-NADP+ reductase from Plasmodium falciparum

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Sep 23 08~~:30:~~06 2009~~''	+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_19736991}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 19736991 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_19736991}}
		+
		+	==About this Structure==
		+	3JQR is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JQR OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:19736991</ref><references group="xtra"/>
		+	[[Category: Plasmodium falciparum]]
		+	[[Category: Bolognesi, M.]]
		+	[[Category: Canevari, G.]]
		+	[[Category: Milani, M.]]
		+	[[Category: Fad]]
		+	[[Category: Ferredoxin-nadp+ reductase]]
		+	[[Category: Oxidoreductase]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 13 14:10:15 2010''

OCA at 05:30, 23 September 2009

OCA — Wed, 23 Sep 2009 05:30:07 GMT

←Older revision		Revision as of 05:30, 23 September 2009
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3jqr is ON HOLD	+	The entry 3jqr is ON HOLD until Paper Publication

-	Authors: Canevari, ~~Giulia~~, Milani, ~~Mario~~, Bolognesi, ~~Martino~~	+	Authors: Canevari, G., Milani, M., Bolognesi, M.

	Description: Crystal structure of the H286L mutant of Ferredoxin-NADP+ reductase from Plasmodium falciparum		Description: Crystal structure of the H286L mutant of Ferredoxin-NADP+ reductase from Plasmodium falciparum

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 16 08~~:45~~:30 2009''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 23 08:30:06 2009''

OCA: New page: '''Unreleased structure''' The entry 3jqr is ON HOLD Authors: Canevari, Giulia, Milani, Mario, Bolognesi, Martino Description: Crystal structure of the H286L mutant of Ferredoxin-NADP+...

OCA — Wed, 16 Sep 2009 05:45:30 GMT

New page: '''Unreleased structure''' The entry 3jqr is ON HOLD Authors: Canevari, Giulia, Milani, Mario, Bolognesi, Martino Description: Crystal structure of the H286L mutant of Ferredoxin-NADP+...

New page

'''Unreleased structure'''

The entry 3jqr is ON HOLD

Authors: Canevari, Giulia, Milani, Mario, Bolognesi, Martino

Description: Crystal structure of the H286L mutant of Ferredoxin-NADP+ reductase from Plasmodium falciparum

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 16 08:45:30 2009''