3k74 - Revision history

OCA at 08:10, 6 September 2023

OCA — Wed, 06 Sep 2023 08:10:31 GMT

←Older revision		Revision as of 08:10, 6 September 2023
Line 3:		Line 3:
	<StructureSection load='3k74' size='340' side='right'caption='[[3k74]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='3k74' size='340' side='right'caption='[[3k74]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3k74]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Camelus_glama Camelus glama~~] and [https://en.wikipedia.org/wiki/~~Ecoli Ecoli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K74 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3K74 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3k74]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12] and [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K74 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3K74 FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[5dfr\|5dfr]], [[1hcv\|1hcv]]</div></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.95Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">b0048, DHFR, folA, JW0047, tmrA ([https://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=83333 ECOLI]), Lama ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9844 Camelus glama])</td></tr>~~	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Dihydrofolate_reductase Dihydrofolate reductase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.3 1.5.1.3] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3k74 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k74 OCA], [https://pdbe.org/3k74 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3k74 RCSB], [https://www.ebi.ac.uk/pdbsum/3k74 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3k74 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3k74 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k74 OCA], [https://pdbe.org/3k74 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3k74 RCSB], [https://www.ebi.ac.uk/pdbsum/3k74 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3k74 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/DYR_ECOLI DYR_ECOLI]] Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.	+	[https://www.uniprot.org/uniprot/DYR_ECOLI DYR_ECOLI] Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 27:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Camelus glama~~]]	+	[[Category: Escherichia coli K-12]]
-	[[Category: ~~Dihydrofolate reductase]]~~	+	[[Category: Lama glama]]
-	~~[[Category: Ecoli~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Barlow, J]]	+	[[Category: Barlow J]]
-	[[Category: Oyen, D]]	+	[[Category: Oyen D]]
-	[[Category: Srinivasan, V]]	+	[[Category: Srinivasan V]]
-	[[Category: Steyaert, J]]	+	[[Category: Steyaert J]]
-	~~[[Category: Antibiotic resistance]]~~	+
-	~~[[Category: Immunoglobulin]]~~	+
-	~~[[Category: Methotrexate resistance]]~~	+
-	~~[[Category: Nadp]]~~	+
-	~~[[Category: One-carbon metabolism]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Protein-nanobody complex]]~~	+
-	~~[[Category: Trimethoprim resistance~~]]	+

OCA at 13:33, 4 May 2022

OCA — Wed, 04 May 2022 13:33:49 GMT

←Older revision		Revision as of 13:33, 4 May 2022
Line 1:		Line 1:

	==Disruption of protein dynamics by an allosteric effector antibody==		==Disruption of protein dynamics by an allosteric effector antibody==
-	<StructureSection load='3k74' size='340' side='right' caption='[[3k74]], [[Resolution\|resolution]] 1.95Å' scene=''>	+	<StructureSection load='3k74' size='340' side='right'caption='[[3k74]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3k74]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Camelus_glama Camelus glama] and [~~http~~://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K74 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3K74 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3k74]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Camelus_glama Camelus glama] and [https://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K74 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3K74 FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5dfr\|5dfr]], [[1hcv\|1hcv]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[5dfr\|5dfr]], [[1hcv\|1hcv]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b0048, DHFR, folA, JW0047, tmrA ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI]), Lama ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9844 Camelus glama])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b0048, DHFR, folA, JW0047, tmrA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI]), Lama ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9844 Camelus glama])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Dihydrofolate_reductase Dihydrofolate reductase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.3 1.5.1.3] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Dihydrofolate_reductase Dihydrofolate reductase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.3 1.5.1.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3k74 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k74 OCA], [~~http~~://pdbe.org/3k74 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3k74 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3k74 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3k74 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3k74 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k74 OCA], [https://pdbe.org/3k74 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3k74 RCSB], [https://www.ebi.ac.uk/pdbsum/3k74 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3k74 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/DYR_ECOLI DYR_ECOLI]] Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.	+	[[https://www.uniprot.org/uniprot/DYR_ECOLI DYR_ECOLI]] Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 20:
	</div>		</div>
	<div class="pdbe-citations 3k74" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3k74" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Dihydrofolate reductase 3D structures\|Dihydrofolate reductase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 27:		Line 30:
	[[Category: Dihydrofolate reductase]]		[[Category: Dihydrofolate reductase]]
	[[Category: Ecoli]]		[[Category: Ecoli]]
		+	[[Category: Large Structures]]
	[[Category: Barlow, J]]		[[Category: Barlow, J]]
	[[Category: Oyen, D]]		[[Category: Oyen, D]]

OCA at 08:27, 1 November 2017

OCA — Wed, 01 Nov 2017 08:27:59 GMT

←Older revision		Revision as of 08:27, 1 November 2017
Line 20:		Line 20:
	</div>		</div>
	<div class="pdbe-citations 3k74" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3k74" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Dihydrofolate reductase\|Dihydrofolate reductase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 16:24, 5 August 2016

OCA — Fri, 05 Aug 2016 16:24:15 GMT

←Older revision		Revision as of 16:24, 5 August 2016
Line 1:		Line 1:
		+
	==Disruption of protein dynamics by an allosteric effector antibody==		==Disruption of protein dynamics by an allosteric effector antibody==
	<StructureSection load='3k74' size='340' side='right' caption='[[3k74]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='3k74' size='340' side='right' caption='[[3k74]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3k74]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli_k-12 Escherichia coli k-12~~] and [http://en.wikipedia.org/wiki/~~Lama_glama Lama glama~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K74 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3K74 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3k74]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Camelus_glama Camelus glama] and [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K74 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3K74 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5dfr\|5dfr]], [[1hcv\|1hcv]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5dfr\|5dfr]], [[1hcv\|1hcv]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b0048, DHFR, folA, JW0047, tmrA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ~~Escherichia coli K-12~~]), Lama ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9844 ~~Lama~~ glama])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b0048, DHFR, folA, JW0047, tmrA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI]), Lama ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9844 Camelus glama])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dihydrofolate_reductase Dihydrofolate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.3 1.5.1.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dihydrofolate_reductase Dihydrofolate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.3 1.5.1.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3k74 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k74 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3k74 RCSB], [http://www.ebi.ac.uk/pdbsum/3k74 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3k74 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k74 OCA], [http://pdbe.org/3k74 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3k74 RCSB], [http://www.ebi.ac.uk/pdbsum/3k74 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3k74 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3k74" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 25:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Camelus glama]]
	[[Category: Dihydrofolate reductase]]		[[Category: Dihydrofolate reductase]]
-	[[Category: ~~Escherichia coli k-12]]~~	+	[[Category: Ecoli]]
-	~~[[Category: Lama glama~~]]	+
	[[Category: Barlow, J]]		[[Category: Barlow, J]]
	[[Category: Oyen, D]]		[[Category: Oyen, D]]

OCA at 03:57, 25 December 2014

OCA — Thu, 25 Dec 2014 03:57:45 GMT

←Older revision		Revision as of 03:57, 25 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3k74 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k74 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3k74 RCSB], [http://www.ebi.ac.uk/pdbsum/3k74 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3k74 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k74 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3k74 RCSB], [http://www.ebi.ac.uk/pdbsum/3k74 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/DYR_ECOLI DYR_ECOLI]] Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 17:03, 18 December 2014

OCA — Thu, 18 Dec 2014 17:03:58 GMT

←Older revision		Revision as of 17:03, 18 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3k74\| PDB=3k74 \| SCENE= }}~~	+	==Disruption of protein dynamics by an allosteric effector antibody==
-	===Disruption of protein dynamics by an allosteric effector antibody===	+	<StructureSection load='3k74' size='340' side='right' caption='[[3k74]], [[Resolution\|resolution]] 1.95Å' scene=''>
-	~~{{ABSTRACT_PUBMED_21238460}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3k74]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12] and [http://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K74 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3K74 FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5dfr\|5dfr]], [[1hcv\|1hcv]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b0048, DHFR, folA, JW0047, tmrA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 Escherichia coli K-12]), Lama ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9844 Lama glama])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dihydrofolate_reductase Dihydrofolate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.3 1.5.1.3] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3k74 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k74 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3k74 RCSB], [http://www.ebi.ac.uk/pdbsum/3k74 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Although it has been known for many years that antibodies display properties characteristic of allosteric effectors, the molecular mechanisms responsible for these effects remain poorly understood. Here, we describe a single-domain antibody fragment (nanobody) that modulates protein function by constraining conformational change in the enzyme dihydrofolate reductase (DHFR). Nanobody 216 (Nb216) behaves as a potent allosteric inhibitor of DHFR, giving rise to mixed hyperbolic inhibition kinetics. The crystal structure of Nb216 in complex with DHFR reveals that the nanobody binds adjacent to the active site. Half of the epitope consists of residues from the flexible Met20 loop. This loop, which ordinarily oscillates between occluded and closed conformations during catalysis, assumes the occluded conformation in the Nb216-bound state. Using stopped flow, we show that Nb216 inhibits DHFR by stabilising the occluded Met20 loop conformation. Surprisingly, kinetic data indicate that the Met20 loop retains sufficient conformational flexibility in the Nb216-bound state to allow slow substrate turnover to occur.

-	~~==Function==~~	+	Constraining enzyme conformational change by an antibody leads to hyperbolic inhibition.,Oyen D, Srinivasan V, Steyaert J, Barlow JN J Mol Biol. 2011 Mar 18;407(1):138-48. Epub 2011 Jan 14. PMID:21238460<ref>PMID:21238460</ref>
-	~~[[http://www.uniprot.org/uniprot/DYR_ECOLI DYR_ECOLI]] Key~~ enzyme ~~in folate metabolism. Catalyzes~~ an ~~essential reaction for de novo glycine and purine synthesis~~, ~~and for DNA precursor synthesis~~.	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[3k74]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli_k-12 Escherichia coli k-12] and [http://en~~.~~wikipedia.org~~/~~wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K74 OCA].~~	+	</div>

	==See Also==		==See Also==
	*[[Dihydrofolate reductase\|Dihydrofolate reductase]]		*[[Dihydrofolate reductase\|Dihydrofolate reductase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:021238460</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Dihydrofolate reductase]]		[[Category: Dihydrofolate reductase]]
	[[Category: Escherichia coli k-12]]		[[Category: Escherichia coli k-12]]
	[[Category: Lama glama]]		[[Category: Lama glama]]
-	[[Category: Barlow, J.]]	+	[[Category: Barlow, J]]
-	[[Category: Oyen, D.]]	+	[[Category: Oyen, D]]
-	[[Category: Srinivasan, V.]]	+	[[Category: Srinivasan, V]]
-	[[Category: Steyaert, J.]]	+	[[Category: Steyaert, J]]
	[[Category: Antibiotic resistance]]		[[Category: Antibiotic resistance]]
	[[Category: Immunoglobulin]]		[[Category: Immunoglobulin]]

OCA at 08:45, 4 September 2013

OCA — Wed, 04 Sep 2013 08:45:29 GMT

←Older revision		Revision as of 08:45, 4 September 2013
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-	[[Image:3k74.png\|left\|200px]]
-
	{{STRUCTURE_3k74\| PDB=3k74 \| SCENE= }}		{{STRUCTURE_3k74\| PDB=3k74 \| SCENE= }}
-
	===Disruption of protein dynamics by an allosteric effector antibody===		===Disruption of protein dynamics by an allosteric effector antibody===
		+	{{ABSTRACT_PUBMED_21238460}}

-	~~{{ABSTRACT_PUBMED_021238460}}~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/DYR_ECOLI DYR_ECOLI]] Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.

	==About this Structure==		==About this Structure==
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	==Reference==		==Reference==
-	<ref group="xtra">PMID:021238460</ref><references group="xtra"/>	+	<ref group="xtra">PMID:021238460</ref><references group="xtra"/><references/>
	[[Category: Dihydrofolate reductase]]		[[Category: Dihydrofolate reductase]]
	[[Category: Escherichia coli k-12]]		[[Category: Escherichia coli k-12]]

OCA at 12:25, 20 October 2012

OCA — Sat, 20 Oct 2012 12:25:19 GMT

←Older revision		Revision as of 12:25, 20 October 2012
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	==About this Structure==		==About this Structure==
-	[[3k74]] is a 2 chain structure ~~of [[Dihydrofolate reductase]]~~ with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12] and [http://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K74 OCA].	+	[[3k74]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12] and [http://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K74 OCA].

	==See Also==		==See Also==

OCA at 08:40, 26 July 2012

OCA — Thu, 26 Jul 2012 08:40:18 GMT

←Older revision		Revision as of 08:40, 26 July 2012
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	[[Image:3k74.png\|left\|200px]]		[[Image:3k74.png\|left\|200px]]

-	<!--
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-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
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	{{STRUCTURE_3k74\| PDB=3k74 \| SCENE= }}		{{STRUCTURE_3k74\| PDB=3k74 \| SCENE= }}

	===Disruption of protein dynamics by an allosteric effector antibody===		===Disruption of protein dynamics by an allosteric effector antibody===

		+	{{ABSTRACT_PUBMED_021238460}}

	==About this Structure==		==About this Structure==
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	==See Also==		==See Also==
-	*[[Dihydrofolate reductase]]	+	*[[Dihydrofolate reductase\|Dihydrofolate reductase]]
		+
		+	==Reference==
		+	<ref group="xtra">PMID:021238460</ref><references group="xtra"/>
	[[Category: Dihydrofolate reductase]]		[[Category: Dihydrofolate reductase]]
	[[Category: Escherichia coli k-12]]		[[Category: Escherichia coli k-12]]

OCA: Protected "3k74" [edit=sysop:move=sysop]

OCA — Sun, 26 Dec 2010 21:39:38 GMT

Protected "3k74" [edit=sysop:move=sysop]

←Older revision

Revision as of 21:39, 26 December 2010