3k7t - Revision history

OCA at 08:11, 6 September 2023

OCA — Wed, 06 Sep 2023 08:11:01 GMT

←Older revision		Revision as of 08:11, 6 September 2023
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	==Crystal structure of apo-form 6-hydroxy-L-nicotine oxidase, crystal form P3121==		==Crystal structure of apo-form 6-hydroxy-L-nicotine oxidase, crystal form P3121==
-	<StructureSection load='3k7t' size='340' side='right' caption='[[3k7t]], [[Resolution\|resolution]] 2.85Å' scene=''>	+	<StructureSection load='3k7t' size='340' side='right'caption='[[3k7t]], [[Resolution\|resolution]] 2.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3k7t]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_49919 Atcc 49919~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K7T OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3K7T FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3k7t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Paenarthrobacter_nicotinovorans Paenarthrobacter nicotinovorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K7T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3K7T FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GP7:(1R)-2-{[(S)-(2-AMINOETHOXY)(HYDROXY)PHOSPHORYL]OXY}-1-[(PENTADECANOYLOXY)METHYL]ETHYL+(12E)-HEXADECA-9,12-DIENOATE'>GP7</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.85Å</td></tr>
-	~~<tr~~ id~~='related'><td class~~="~~sblockLbl~~">~~<b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3k7m\|3k7m]]~~, [[~~3k7q~~\|~~3k7q~~]]</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GP7:(1R)-2-{[(S)-(2-AMINOETHOXY)(HYDROXY)PHOSPHORYL]OXY}-1-[(PENTADECANOYLOXY)METHYL]ETHYL+(12E)-HEXADECA-9,12-DIENOATE'>GP7</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>6-HLNO ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~29320 ATCC 49919])</td~~><~~/tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3k7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k7t OCA], [https://pdbe.org/3k7t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3k7t RCSB], [https://www.ebi.ac.uk/pdbsum/3k7t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3k7t ProSAT]</span></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks'>[http~~:~~//en.wikipedia.org/wiki/~~(S)-6-~~hydroxynicotine_oxidase~~ (S)-6-~~hydroxynicotine oxidase~~]~~, with EC number [http://www.brenda~~-~~enzymes.info/php/result_flat.php4?ecno=~~1~~.5.3.5 1.5.3.5~~] </~~span~~></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3k7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k7t OCA], [~~http~~://pdbe.org/3k7t PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3k7t RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3k7t PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3k7t ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/HLNO_PAENI HLNO_PAENI] Involved in the degradation of L-nicotine (PubMed:5849820). Catalyzes the oxidation of (S)-6-hydroxynicotine (6-hydroxy-L-nicotine) to 6-hydroxypseudooxynicotine (PubMed:5849820, PubMed:4965794, PubMed:5646150, PubMed:21383134, PubMed:26744768, PubMed:28080034). Oxidation of the pyrrolidine ring of (S)-6-hydroxynicotine leads to the formation of the optically inactive 6-hydroxy-N-methylmyosmine, which hydrolyzes spontaneously to 6-hydroxypseudooxynicotine (PubMed:4965794, PubMed:21383134, PubMed:26744768, PubMed:28080034). Acts with absolute stereospecificity on the L-form of 6-hydroxynicotine (PubMed:4965794). Can also use (S)-6-hydroxynornicotine (PubMed:26744768, PubMed:28080034).<ref>PMID:21383134</ref> <ref>PMID:26744768</ref> <ref>PMID:28080034</ref> <ref>PMID:4965794</ref> <ref>PMID:5646150</ref> <ref>PMID:5849820</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k7/3k7t_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k7/3k7t_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 33:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Atcc 49919~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Bartunik, H D~~]]	+	[[Category: Paenarthrobacter nicotinovorans]]
-	[[Category: ~~Bourenkov, G P~~]]	+	[[Category: Bartunik HD]]
-	[[Category: ~~Kachalova, G S~~]]	+	[[Category: Bourenkov GP]]
-	[[Category: ~~Enantiomeric substrate]]~~	+	[[Category: Kachalova GS]]
-	~~[[Category: Flavoenzyme]]~~	+
-	~~[[Category: Nicotine degradation]]~~	+
-	~~[[Category: Oxidase]]~~	+
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 08:28, 1 November 2017

OCA — Wed, 01 Nov 2017 08:28:32 GMT

←Older revision		Revision as of 08:28, 1 November 2017
Line 1:		Line 1:
		+
	==Crystal structure of apo-form 6-hydroxy-L-nicotine oxidase, crystal form P3121==		==Crystal structure of apo-form 6-hydroxy-L-nicotine oxidase, crystal form P3121==
	<StructureSection load='3k7t' size='340' side='right' caption='[[3k7t]], [[Resolution\|resolution]] 2.85Å' scene=''>		<StructureSection load='3k7t' size='340' side='right' caption='[[3k7t]], [[Resolution\|resolution]] 2.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3k7t]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Artni Artni~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K7T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3K7T FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3k7t]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_49919 Atcc 49919]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K7T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3K7T FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GP7:(1R)-2-{[(S)-(2-AMINOETHOXY)(HYDROXY)PHOSPHORYL]OXY}-1-[(PENTADECANOYLOXY)METHYL]ETHYL+(12E)-HEXADECA-9,12-DIENOATE'>GP7</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GP7:(1R)-2-{[(S)-(2-AMINOETHOXY)(HYDROXY)PHOSPHORYL]OXY}-1-[(PENTADECANOYLOXY)METHYL]ETHYL+(12E)-HEXADECA-9,12-DIENOATE'>GP7</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3k7m\|3k7m]], [[3k7q\|3k7q]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3k7m\|3k7m]], [[3k7q\|3k7q]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">6-HLNO ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=29320 ~~ARTNI~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">6-HLNO ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=29320 ATCC 49919])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/(S)-6-hydroxynicotine_oxidase (S)-6-hydroxynicotine oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.3.5 1.5.3.5] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/(S)-6-hydroxynicotine_oxidase (S)-6-hydroxynicotine oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.3.5 1.5.3.5] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3k7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k7t OCA], [http://pdbe.org/3k7t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3k7t RCSB], [http://www.ebi.ac.uk/pdbsum/3k7t PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3k7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k7t OCA], [http://pdbe.org/3k7t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3k7t RCSB], [http://www.ebi.ac.uk/pdbsum/3k7t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3k7t ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 32:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Artni~~]]	+	[[Category: Atcc 49919]]
	[[Category: Bartunik, H D]]		[[Category: Bartunik, H D]]
	[[Category: Bourenkov, G P]]		[[Category: Bourenkov, G P]]

OCA at 18:33, 9 February 2016

OCA — Tue, 09 Feb 2016 18:33:59 GMT

←Older revision		Revision as of 18:33, 9 February 2016
Line 2:		Line 2:
	<StructureSection load='3k7t' size='340' side='right' caption='[[3k7t]], [[Resolution\|resolution]] 2.85Å' scene=''>		<StructureSection load='3k7t' size='340' side='right' caption='[[3k7t]], [[Resolution\|resolution]] 2.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3k7t]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Arthrobacter_nicotinovorans Arthrobacter nicotinovorans~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K7T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3K7T FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3k7t]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Artni Artni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K7T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3K7T FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GP7:(1R)-2-{[(S)-(2-AMINOETHOXY)(HYDROXY)PHOSPHORYL]OXY}-1-[(PENTADECANOYLOXY)METHYL]ETHYL+(12E)-HEXADECA-9,12-DIENOATE'>GP7</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GP7:(1R)-2-{[(S)-(2-AMINOETHOXY)(HYDROXY)PHOSPHORYL]OXY}-1-[(PENTADECANOYLOXY)METHYL]ETHYL+(12E)-HEXADECA-9,12-DIENOATE'>GP7</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3k7m\|3k7m]], [[3k7q\|3k7q]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3k7m\|3k7m]], [[3k7q\|3k7q]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">6-HLNO ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=29320 ~~Arthrobacter nicotinovorans~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">6-HLNO ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=29320 ARTNI])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/(S)-6-hydroxynicotine_oxidase (S)-6-hydroxynicotine oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.3.5 1.5.3.5] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/(S)-6-hydroxynicotine_oxidase (S)-6-hydroxynicotine oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.3.5 1.5.3.5] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3k7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k7t OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3k7t RCSB], [http://www.ebi.ac.uk/pdbsum/3k7t PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3k7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k7t OCA], [http://pdbe.org/3k7t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3k7t RCSB], [http://www.ebi.ac.uk/pdbsum/3k7t PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3k7t ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3k7t" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Arthrobacter nicotinovorans~~]]	+	[[Category: Artni]]
	[[Category: Bartunik, H D]]		[[Category: Bartunik, H D]]
	[[Category: Bourenkov, G P]]		[[Category: Bourenkov, G P]]

OCA at 17:32, 18 December 2014

OCA — Thu, 18 Dec 2014 17:32:19 GMT

←Older revision		Revision as of 17:32, 18 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3k7t\| PDB=3k7t \| SCENE= }}~~	+	==Crystal structure of apo-form 6-hydroxy-L-nicotine oxidase, crystal form P3121==
-	===Crystal structure of apo-form 6-hydroxy-L-nicotine oxidase, crystal form P3121===	+	<StructureSection load='3k7t' size='340' side='right' caption='[[3k7t]], [[Resolution\|resolution]] 2.85Å' scene=''>
-	{~~{ABSTRACT_PUBMED_20006620}~~}	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3k7t]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arthrobacter_nicotinovorans Arthrobacter nicotinovorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K7T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3K7T FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GP7:(1R)-2-{[(S)-(2-AMINOETHOXY)(HYDROXY)PHOSPHORYL]OXY}-1-[(PENTADECANOYLOXY)METHYL]ETHYL+(12E)-HEXADECA-9,12-DIENOATE'>GP7</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3k7m\|3k7m]], [[3k7q\|3k7q]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">6-HLNO ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=29320 Arthrobacter nicotinovorans])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/(S)-6-hydroxynicotine_oxidase (S)-6-hydroxynicotine oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.3.5 1.5.3.5] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3k7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k7t OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3k7t RCSB], [http://www.ebi.ac.uk/pdbsum/3k7t PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k7/3k7t_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The pathway for oxidative degradation of nicotine in Arthrobacter nicotinovorans includes two genetically and structurally unrelated flavoenzymes, 6-hydroxy-L-nicotine oxidase (6HLNO) and 6-hydroxy-D-nicotine oxidase, which act with absolute stereospecificity on the L- and D-forms, respectively, of 6-hydroxy-nicotine. We solved the crystal structure of 6HLNO at 1.95 A resolution by combined isomorphous/multiple-wavelength anomalous dispersion phasing. The overall structure of each subunit of the 6HLNO homodimer and the folds of the individual domains are closely similar as in eukaryotic monoamine oxidases. Unexpectedly, a diacylglycerophospholipid molecule was found to be non-covalently bound to each protomer of 6HLNO. The fatty acid chains occupy hydrophobic channels that penetrate deep into the interior of the substrate-binding domain of each subunit. The solvent-exposed glycerophosphate moiety is located at the subunit-subunit interface. We further solved the crystal structure of a complex of dithionite-reduced 6HLNO with the natural substrate 6-hydroxy-L-nicotine at 2.05 A resolution. The location of the substrate in a tight cavity suggests that the binding geometry of this unproductive complex may be closely similar as under oxidizing conditions. The observed orientation of the bound substrate relative to the isoalloxazine ring of the flavin adenine dinucleotide cofactor is suitable for hydride-transfer dehydrogenation at the carbon atom that forms the chiral center of the substrate molecule. A comparison of the substrate-binding modes of 6HLNO and 6-hydroxy-D-nicotine oxidase, based on models of complexes with the D-substrate, suggests an explanation for the stereospecificity of both enzymes. The two enzymes are proposed to orient the enantiomeric substrates in mirror symmetry with respect to the plane of the flavin.

-	~~==About this Structure==~~	+	Crystal structure analysis of free and substrate-bound 6-hydroxy-L-nicotine oxidase from Arthrobacter nicotinovorans.,Kachalova GS, Bourenkov GP, Mengesdorf T, Schenk S, Maun HR, Burghammer M, Riekel C, Decker K, Bartunik HD J Mol Biol. 2010 Feb 26;396(3):785-99. Epub 2009 Dec 16. PMID:20006620<ref>PMID:20006620</ref>
-	~~[[3k7t]] is a 2 chain~~ structure ~~with sequence~~ from ~~[http://en.wikipedia.org/wiki/Arthrobacter_nicotinovorans~~ Arthrobacter nicotinovorans]. ~~Full crystallographic information is available from [http~~:~~//oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=3K7T OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:020006620</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Arthrobacter nicotinovorans]]		[[Category: Arthrobacter nicotinovorans]]
-	[[Category: Bartunik, H D.]]	+	[[Category: Bartunik, H D]]
-	[[Category: Bourenkov, G P.]]	+	[[Category: Bourenkov, G P]]
-	[[Category: Kachalova, G S.]]	+	[[Category: Kachalova, G S]]
	[[Category: Enantiomeric substrate]]		[[Category: Enantiomeric substrate]]
	[[Category: Flavoenzyme]]		[[Category: Flavoenzyme]]

OCA: Protected "3k7t" [edit=sysop:move=sysop]

OCA — Thu, 04 Apr 2013 02:27:47 GMT

Protected "3k7t" [edit=sysop:move=sysop]

←Older revision

Revision as of 02:27, 4 April 2013

OCA at 02:27, 4 April 2013

OCA — Thu, 04 Apr 2013 02:27:46 GMT

←Older revision		Revision as of 02:27, 4 April 2013
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-	{{Seed}}
-	[[Image:3k7t.png\|left\|200px]]
-
-	<!--
-	The line below this paragraph, containing "STRUCTURE_3k7t", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3k7t\| PDB=3k7t \| SCENE= }}		{{STRUCTURE_3k7t\| PDB=3k7t \| SCENE= }}
-
	===Crystal structure of apo-form 6-hydroxy-L-nicotine oxidase, crystal form P3121===		===Crystal structure of apo-form 6-hydroxy-L-nicotine oxidase, crystal form P3121===
-
-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_20006620}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 20006620 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_20006620}}		{{ABSTRACT_PUBMED_20006620}}

	==About this Structure==		==About this Structure==
-	~~3K7T~~ is a 2 ~~chains~~ structure with ~~sequences~~ from [http://en.wikipedia.org/wiki/Arthrobacter_nicotinovorans Arthrobacter nicotinovorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K7T OCA].	+	[[3k7t]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arthrobacter_nicotinovorans Arthrobacter nicotinovorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K7T OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~20006620~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:020006620</ref><references group="xtra"/><references/>
	[[Category: Arthrobacter nicotinovorans]]		[[Category: Arthrobacter nicotinovorans]]
	[[Category: Bartunik, H D.]]		[[Category: Bartunik, H D.]]
Line 33:		Line 17:
	[[Category: Oxidase]]		[[Category: Oxidase]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 3 13:07:10 2010''

OCA at 11:07, 3 March 2010

OCA — Wed, 03 Mar 2010 11:07:11 GMT

←Older revision		Revision as of 11:07, 3 March 2010
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	{{Seed}}		{{Seed}}
-	[[Image:3k7t.~~jpg~~\|left\|200px]]	+	[[Image:3k7t.png\|left\|200px]]

	<!--		<!--
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	[[Category: Nicotine degradation]]		[[Category: Nicotine degradation]]
	[[Category: Oxidase]]		[[Category: Oxidase]]
		+	[[Category: Oxidoreductase]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Jan 20 15~~:56:33 2010''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 3 13:07:10 2010''

OCA at 13:56, 20 January 2010

OCA — Wed, 20 Jan 2010 13:56:35 GMT

←Older revision		Revision as of 13:56, 20 January 2010
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3k7t.jpg\|left\|200px]]

-	The ~~entry 3k7t~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3k7t", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3k7t\| PDB=3k7t \| SCENE= }}

-	~~Authors: Bourenkov~~, ~~G.P., Kachalova, G.S., Bartunik, H.D.~~	+	===Crystal structure of apo-form 6-hydroxy-L-nicotine oxidase, crystal form P3121===

-	Description: Crystal structure of apo-form 6-hydroxy-L-nicotine oxidase, crystal form P3121

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Oct 28 12~~:58:~~15 2009~~''	+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_20006620}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 20006620 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_20006620}}
		+
		+	==About this Structure==
		+	3K7T is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Arthrobacter_nicotinovorans Arthrobacter nicotinovorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K7T OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:20006620</ref><references group="xtra"/>
		+	[[Category: Arthrobacter nicotinovorans]]
		+	[[Category: Bartunik, H D.]]
		+	[[Category: Bourenkov, G P.]]
		+	[[Category: Kachalova, G S.]]
		+	[[Category: Enantiomeric substrate]]
		+	[[Category: Flavoenzyme]]
		+	[[Category: Nicotine degradation]]
		+	[[Category: Oxidase]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 20 15:56:33 2010''

OCA at 10:58, 28 October 2009

OCA — Wed, 28 Oct 2009 10:58:16 GMT

←Older revision		Revision as of 10:58, 28 October 2009
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3k7t is ON HOLD	+	The entry 3k7t is ON HOLD until Paper Publication

	Authors: Bourenkov, G.P., Kachalova, G.S., Bartunik, H.D.		Authors: Bourenkov, G.P., Kachalova, G.S., Bartunik, H.D.
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	Description: Crystal structure of apo-form 6-hydroxy-L-nicotine oxidase, crystal form P3121		Description: Crystal structure of apo-form 6-hydroxy-L-nicotine oxidase, crystal form P3121

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct ~~21 10~~:01:26 2009''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct 28 12:58:15 2009''

OCA: New page: '''Unreleased structure''' The entry 3k7t is ON HOLD Authors: Bourenkov, G.P., Kachalova, G.S., Bartunik, H.D. Description: Crystal structure of apo-form 6-hydroxy-L-nicotine oxidase, ...

OCA — Wed, 21 Oct 2009 08:01:26 GMT

New page: '''Unreleased structure''' The entry 3k7t is ON HOLD Authors: Bourenkov, G.P., Kachalova, G.S., Bartunik, H.D. Description: Crystal structure of apo-form 6-hydroxy-L-nicotine oxidase, ...

New page

'''Unreleased structure'''

The entry 3k7t is ON HOLD

Authors: Bourenkov, G.P., Kachalova, G.S., Bartunik, H.D.

Description: Crystal structure of apo-form 6-hydroxy-L-nicotine oxidase, crystal form P3121

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct 21 10:01:26 2009''