3k7v - Revision history

OCA at 08:11, 6 September 2023

OCA — Wed, 06 Sep 2023 08:11:10 GMT

←Older revision		Revision as of 08:11, 6 September 2023
Line 1:		Line 1:

	==Protein phosphatase 2A core complex bound to dinophysistoxin-1==		==Protein phosphatase 2A core complex bound to dinophysistoxin-1==
-	<StructureSection load='3k7v' size='340' side='right' caption='[[3k7v]], [[Resolution\|resolution]] 2.85Å' scene=''>	+	<StructureSection load='3k7v' size='340' side='right'caption='[[3k7v]], [[Resolution\|resolution]] 2.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3k7v]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K7V OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3K7V FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3k7v]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K7V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3K7V FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=XT1:(2R)-3-[(2S,5R,6R,8S)-8-{(1R,2E)-3-[(2R,4AR,5R,6S,8R,8AS)-6-{(1S,3S)-3-[(2S,3R,6R,11R)-3,11-DIMETHYL-1,7-DIOXASPIRO[5.5]UNDEC-2-YL]-1-HYDROXYBUTYL}-8-HYDROXY-7-METHYLIDENEOCTAHYDRO-3H,3H-SPIRO[FURAN-2,2-PYRANO[3,2-B]PYRAN]-5-YL]-1-METHYLPROP-2-EN-1-YL}-5-HYDROXY-10-METHYL-1,7-DIOXASPIRO[5.5]UNDEC-10-EN-2-YL]-2-HYDROXY-2-METHYLPROPANOIC+ACID'>XT1</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.85Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ie4\|2ie4]], [[3k7w\|3k7w]]</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=XT1:(2R)-3-[(2S,5R,6R,8S)-8-{(1R,2E)-3-[(2R,4AR,5R,6S,8R,8AS)-6-{(1S,3S)-3-[(2S,3R,6R,11R)-3,11-DIMETHYL-1,7-DIOXASPIRO[5.5]UNDEC-2-YL]-1-HYDROXYBUTYL}-8-HYDROXY-7-METHYLIDENEOCTAHYDRO-3H,3H-SPIRO[FURAN-2,2-PYRANO[3,2-B]PYRAN]-5-YL]-1-METHYLPROP-2-EN-1-YL}-5-HYDROXY-10-METHYL-1,7-DIOXASPIRO[5.5]UNDEC-10-EN-2-YL]-2-HYDROXY-2-METHYLPROPANOIC+ACID'>XT1</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PPP2R1A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), PPP2CA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3k7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k7v OCA], [https://pdbe.org/3k7v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3k7v RCSB], [https://www.ebi.ac.uk/pdbsum/3k7v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3k7v ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoprotein_phosphatase Phosphoprotein phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.16 3.1.3.16] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3k7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k7v OCA], [~~http~~://pdbe.org/3k7v PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3k7v RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3k7v PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3k7v ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/2AAA_HUMAN 2AAA_HUMAN]] The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Required for proper chromosome segregation and for centromeric localization of SGOL1 in mitosis.<ref>PMID:16580887</ref> [[http://www.uniprot.org/uniprot/PP2AA_HUMAN PP2AA_HUMAN]] PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser-259'.<ref>PMID:9920888</ref> <ref>PMID:10801873</ref> <ref>PMID:22613722</ref>	+	[https://www.uniprot.org/uniprot/2AAA_HUMAN 2AAA_HUMAN] The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Required for proper chromosome segregation and for centromeric localization of SGOL1 in mitosis.<ref>PMID:16580887</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k7/3k7v_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k7/3k7v_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 34:		Line 32:
	==See Also==		==See Also==
	*[[Protein phosphatase\|Protein phosphatase]]		*[[Protein phosphatase\|Protein phosphatase]]
-	*[[Serine/threonine protein phosphatase\|Serine/threonine protein phosphatase]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
-	[[Category: ~~Phosphoprotein phosphatase~~]]	+	[[Category: Large Structures]]
-	[[Category: Huhn, J]]	+	[[Category: Huhn J]]
-	[[Category: Jeffrey~~, P D~~]]	+	[[Category: Jeffrey PD]]
-	[[Category: Shi, Y]]	+	[[Category: Shi Y]]
-	~~[[Category: Centromere]]~~	+
-	~~[[Category: Cytoskeleton]]~~	+
-	~~[[Category: Heat repeat]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Iron]]~~	+
-	~~[[Category: Ligand specificity]]~~	+
-	~~[[Category: Manganese]]~~	+
-	~~[[Category: Metal-binding]]~~	+
-	~~[[Category: Methylation]]~~	+
-	~~[[Category: Nucleus]]~~	+
-	~~[[Category: Phosphatase]]~~	+
-	~~[[Category: Phosphoprotein]]~~	+
-	~~[[Category: Protein phosphatase]]~~	+
-	~~[[Category: Protein-protein complex]]~~	+
-	~~[[Category: Signaling protein]]~~	+
-	~~[[Category: Signaling protein-hydrolase complex~~]]	+

OCA at 04:38, 5 August 2016

OCA — Fri, 05 Aug 2016 04:38:52 GMT

←Older revision		Revision as of 04:38, 5 August 2016
Line 1:		Line 1:
		+
	==Protein phosphatase 2A core complex bound to dinophysistoxin-1==		==Protein phosphatase 2A core complex bound to dinophysistoxin-1==
	<StructureSection load='3k7v' size='340' side='right' caption='[[3k7v]], [[Resolution\|resolution]] 2.85Å' scene=''>		<StructureSection load='3k7v' size='340' side='right' caption='[[3k7v]], [[Resolution\|resolution]] 2.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3k7v]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K7V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3K7V FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3k7v]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K7V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3K7V FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=XT1:(2R)-3-[(2S,5R,6R,8S)-8-{(1R,2E)-3-[(2R,4AR,5R,6S,8R,8AS)-6-{(1S,3S)-3-[(2S,3R,6R,11R)-3,11-DIMETHYL-1,7-DIOXASPIRO[5.5]UNDEC-2-YL]-1-HYDROXYBUTYL}-8-HYDROXY-7-METHYLIDENEOCTAHYDRO-3H,3H-SPIRO[FURAN-2,2-PYRANO[3,2-B]PYRAN]-5-YL]-1-METHYLPROP-2-EN-1-YL}-5-HYDROXY-10-METHYL-1,7-DIOXASPIRO[5.5]UNDEC-10-EN-2-YL]-2-HYDROXY-2-METHYLPROPANOIC+ACID'>XT1</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=XT1:(2R)-3-[(2S,5R,6R,8S)-8-{(1R,2E)-3-[(2R,4AR,5R,6S,8R,8AS)-6-{(1S,3S)-3-[(2S,3R,6R,11R)-3,11-DIMETHYL-1,7-DIOXASPIRO[5.5]UNDEC-2-YL]-1-HYDROXYBUTYL}-8-HYDROXY-7-METHYLIDENEOCTAHYDRO-3H,3H-SPIRO[FURAN-2,2-PYRANO[3,2-B]PYRAN]-5-YL]-1-METHYLPROP-2-EN-1-YL}-5-HYDROXY-10-METHYL-1,7-DIOXASPIRO[5.5]UNDEC-10-EN-2-YL]-2-HYDROXY-2-METHYLPROPANOIC+ACID'>XT1</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ie4\|2ie4]], [[3k7w\|3k7w]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ie4\|2ie4]], [[3k7w\|3k7w]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PPP2R1A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~]), PPP2CA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PPP2R1A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), PPP2CA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoprotein_phosphatase Phosphoprotein phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.16 3.1.3.16] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoprotein_phosphatase Phosphoprotein phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.16 3.1.3.16] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3k7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k7v OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3k7v RCSB], [http://www.ebi.ac.uk/pdbsum/3k7v PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3k7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k7v OCA], [http://pdbe.org/3k7v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3k7v RCSB], [http://www.ebi.ac.uk/pdbsum/3k7v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3k7v ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3k7v ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 29:		Line 30:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3k7v" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 37:		Line 39:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Phosphoprotein phosphatase]]		[[Category: Phosphoprotein phosphatase]]
	[[Category: Huhn, J]]		[[Category: Huhn, J]]

OCA at 15:51, 25 December 2014

OCA — Thu, 25 Dec 2014 15:51:37 GMT

←Older revision		Revision as of 15:51, 25 December 2014
Line 9:		Line 9:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3k7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k7v OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3k7v RCSB], [http://www.ebi.ac.uk/pdbsum/3k7v PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3k7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k7v OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3k7v RCSB], [http://www.ebi.ac.uk/pdbsum/3k7v PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/2AAA_HUMAN 2AAA_HUMAN]] The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Required for proper chromosome segregation and for centromeric localization of SGOL1 in mitosis.<ref>PMID:16580887</ref> [[http://www.uniprot.org/uniprot/PP2AA_HUMAN PP2AA_HUMAN]] PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser-259'.<ref>PMID:9920888</ref> <ref>PMID:10801873</ref> <ref>PMID:22613722</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 16:13, 18 December 2014

OCA — Thu, 18 Dec 2014 16:13:28 GMT

←Older revision		Revision as of 16:13, 18 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3k7v\| PDB=3k7v \| SCENE= }}~~	+	==Protein phosphatase 2A core complex bound to dinophysistoxin-1==
-	===Protein phosphatase 2A core complex bound to dinophysistoxin-1===	+	<StructureSection load='3k7v' size='340' side='right' caption='[[3k7v]], [[Resolution\|resolution]] 2.85Å' scene=''>
-	{{~~ABSTRACT_PUBMED_19916524~~}}	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3k7v]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K7V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3K7V FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=XT1:(2R)-3-[(2S,5R,6R,8S)-8-{(1R,2E)-3-[(2R,4AR,5R,6S,8R,8AS)-6-{(1S,3S)-3-[(2S,3R,6R,11R)-3,11-DIMETHYL-1,7-DIOXASPIRO[5.5]UNDEC-2-YL]-1-HYDROXYBUTYL}-8-HYDROXY-7-METHYLIDENEOCTAHYDRO-3H,3H-SPIRO[FURAN-2,2-PYRANO[3,2-B]PYRAN]-5-YL]-1-METHYLPROP-2-EN-1-YL}-5-HYDROXY-10-METHYL-1,7-DIOXASPIRO[5.5]UNDEC-10-EN-2-YL]-2-HYDROXY-2-METHYLPROPANOIC+ACID'>XT1</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ie4\|2ie4]], [[3k7w\|3k7w]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PPP2R1A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), PPP2CA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoprotein_phosphatase Phosphoprotein phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.16 3.1.3.16] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3k7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k7v OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3k7v RCSB], [http://www.ebi.ac.uk/pdbsum/3k7v PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k7/3k7v_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Okadaic acid (OA), dinophysistoxin-1 (DTX-1), and dinophysistoxin-2 (DTX-2) are algal toxins that can accumulate in shellfish and cause diarrhetic shellfish poisoning. Recent studies indicate that DTX-2 is about half as toxic and has about half the affinity for protein phosphatase 2A (PP2A) as OA. NMR structural studies showed that DTX-1 possessed an equatorial 35-methyl group but that DTX-2 had an axial 35-methyl group. Molecular modeling studies indicated that an axial 35-methyl could exhibit unfavorable interactions in the PP2A binding site, and this has been proposed as the reason for the reduced toxicity of DTX-2. Statistical analyses of published data indicate that the affinity of PP2A for DTX-1 is 1.6-fold higher, and for DTX-2 is 2-fold lower, than for OA. We obtained X-ray crystal structures of DTX-1 and DTX-2 bound to PP2A. The crystal structures independently confirm the C-35 stereochemistries determined in the earlier NMR study. The structure for the DTX-1 complex was virtually identical to that of the OA-PP2A complex, except for the presence of the equatorial 35-methyl on the ligand. The favorable placement of the equatorial 35-methyl group of DTX-1 against the aromatic pi-bonds of His191 may account for the increased affinity of PP2A toward DTX-1. In contrast, the axial 35-methyl of DTX-2 caused the side chain of His191 to rotate 140 degrees so that it pointed toward the solvent, thereby opening one end of the hydrophobic binding cage. This rearrangement to accommodate the unfavorable interaction from the axial 35-methyl of DTX-2 reduces the binding energy and appears to be responsible for the reduced affinity of PP2A for DTX-2. These results highlight the potential of molecular modeling studies for understanding the relative toxicity of analogues once the binding site at the molecular target has been properly characterized.

-	~~==Function==~~	+	A structural basis for the reduced toxicity of dinophysistoxin-2.,Huhn J, Jeffrey PD, Larsen K, Rundberget T, Rise F, Cox NR, Arcus V, Shi Y, Miles CO Chem Res Toxicol. 2009 Nov;22(11):1782-6. PMID:19916524<ref>PMID:19916524</ref>
-	~~[[http://www.uniprot.org/uniprot/2AAA_HUMAN 2AAA_HUMAN]] The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate~~ the ~~assembly~~ of the catalytic subunit and a variable regulatory B subunit. Required for proper chromosome segregation and for centromeric localization of SGOL1 in mitosis.<ref>PMID:16580887</ref> [[http://www.uniprot.org/uniprot/PP2AA_HUMAN PP2AA_HUMAN]] PP2A is the major phosphatase for microtubule-~~associated proteins (MAPs)~~. ~~PP2A can modulate the activity of phosphorylase B kinase casein kinase 2~~, ~~mitogen-stimulated S6 kinase~~, ~~and MAP-2 kinase~~. ~~Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I~~ (~~By similarity~~)~~. Can dephosphorylate SV40 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser~~-~~259'~~.~~<ref>~~PMID:~~9920888</ref> <ref>PMID:10801873</ref>~~ <ref>PMID:~~22613722~~</ref>	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[3k7v]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3K7V OCA].~~	+	</div>

-	==~~Reference~~==	+	==See Also==
-	~~<ref group~~=~~"xtra">PMID:019916524</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	*[[Protein phosphatase\|Protein phosphatase]]
		+	*[[Serine/threonine protein phosphatase\|Serine/threonine protein phosphatase]]
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Phosphoprotein phosphatase]]		[[Category: Phosphoprotein phosphatase]]
-	[[Category: Huhn, J.]]	+	[[Category: Huhn, J]]
-	[[Category: Jeffrey, P D.]]	+	[[Category: Jeffrey, P D]]
-	[[Category: Shi, Y.]]	+	[[Category: Shi, Y]]
	[[Category: Centromere]]		[[Category: Centromere]]
	[[Category: Cytoskeleton]]		[[Category: Cytoskeleton]]

OCA: Protected "3k7v" [edit=sysop:move=sysop]

OCA — Thu, 04 Apr 2013 02:09:46 GMT

Protected "3k7v" [edit=sysop:move=sysop]

←Older revision

Revision as of 02:09, 4 April 2013

OCA at 02:09, 4 April 2013

OCA — Thu, 04 Apr 2013 02:09:42 GMT

←Older revision		Revision as of 02:09, 4 April 2013
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-	{{Seed}}
-	[[Image:3k7v.png\|left\|200px]]
-
-	<!--
-	The line below this paragraph, containing "STRUCTURE_3k7v", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3k7v\| PDB=3k7v \| SCENE= }}		{{STRUCTURE_3k7v\| PDB=3k7v \| SCENE= }}
-
	===Protein phosphatase 2A core complex bound to dinophysistoxin-1===		===Protein phosphatase 2A core complex bound to dinophysistoxin-1===
		+	{{ABSTRACT_PUBMED_19916524}}

-		+	==Function==
-	~~<!--~~	+	[[http://www.uniprot.org/uniprot/2AAA_HUMAN 2AAA_HUMAN]] The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Required for proper chromosome segregation and for centromeric localization of SGOL1 in mitosis.<ref>PMID:16580887</ref> [[http://www.uniprot.org/uniprot/PP2AA_HUMAN PP2AA_HUMAN]] PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser-259'.<ref>PMID:9920888</ref> <ref>PMID:10801873</ref> <ref>PMID:22613722</ref>
-	The ~~line below this paragraph, {{ABSTRACT_PUBMED_19916524}}, adds the Publication Abstract~~ to the ~~page~~	+
-	~~(as it appears on PubMed at~~ http://www.~~pubmed~~.~~gov), where 19916524~~ is the ~~PubMed ID number~~.	+
-	-->	+
-	~~{{ABSTRACT_PUBMED_19916524}}~~	+

	==About this Structure==		==About this Structure==
-	~~3K7V~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K7V OCA].	+	[[3k7v]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K7V OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~19916524~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019916524</ref><references group="xtra"/><references/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Phosphoprotein phosphatase]]		[[Category: Phosphoprotein phosphatase]]
Line 29:		Line 16:
	[[Category: Jeffrey, P D.]]		[[Category: Jeffrey, P D.]]
	[[Category: Shi, Y.]]		[[Category: Shi, Y.]]
-	[[Category: Acetylation]]
	[[Category: Centromere]]		[[Category: Centromere]]
-	[[Category: Cytoplasm]]
	[[Category: Cytoskeleton]]		[[Category: Cytoskeleton]]
	[[Category: Heat repeat]]		[[Category: Heat repeat]]
Line 43:		Line 28:
	[[Category: Phosphatase]]		[[Category: Phosphatase]]
	[[Category: Phosphoprotein]]		[[Category: Phosphoprotein]]
-	[[Category: Polymorphism]]
	[[Category: Protein phosphatase]]		[[Category: Protein phosphatase]]
	[[Category: Protein-protein complex]]		[[Category: Protein-protein complex]]
	[[Category: Signaling protein]]		[[Category: Signaling protein]]
-		+	[[Category: Signaling protein-hydrolase complex]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Wed Dec 2 09:13:14 2009''~~	+

OCA at 07:13, 2 December 2009

OCA — Wed, 02 Dec 2009 07:13:14 GMT

←Older revision		Revision as of 07:13, 2 December 2009
Line 1:		Line 1:
	{{Seed}}		{{Seed}}
-	[[Image:3k7v.~~jpg~~\|left\|200px]]	+	[[Image:3k7v.png\|left\|200px]]

	<!--		<!--
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	===Protein phosphatase 2A core complex bound to dinophysistoxin-1===		===Protein phosphatase 2A core complex bound to dinophysistoxin-1===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_19916524}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 19916524 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_19916524}}

	==About this Structure==		==About this Structure==
	3K7V is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K7V OCA].		3K7V is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K7V OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:19916524</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Phosphoprotein phosphatase]]		[[Category: Phosphoprotein phosphatase]]
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	[[Category: Signaling protein]]		[[Category: Signaling protein]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Nov~~ ~~4 11~~:04:42 2009''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 2 09:13:14 2009''

OCA at 09:04, 4 November 2009

OCA — Wed, 04 Nov 2009 09:04:43 GMT

←Older revision		Revision as of 09:04, 4 November 2009
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3k7v.jpg\|left\|200px]]

-	The ~~entry 3k7v~~ is ~~ON HOLD~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3k7v", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3k7v\| PDB=3k7v \| SCENE= }}

-	~~Authors: Jeffrey, P.D., Huhn, J., Shi, Y.~~	+	===Protein phosphatase 2A core complex bound to dinophysistoxin-1===

-	Description: Protein phosphatase 2A core complex bound to dinophysistoxin-1

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Oct 21 10~~:01:27 2009''	+	==About this Structure==
		+	3K7V is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K7V OCA].
		+	[[Category: Homo sapiens]]
		+	[[Category: Phosphoprotein phosphatase]]
		+	[[Category: Huhn, J.]]
		+	[[Category: Jeffrey, P D.]]
		+	[[Category: Shi, Y.]]
		+	[[Category: Acetylation]]
		+	[[Category: Centromere]]
		+	[[Category: Cytoplasm]]
		+	[[Category: Cytoskeleton]]
		+	[[Category: Heat repeat]]
		+	[[Category: Hydrolase]]
		+	[[Category: Iron]]
		+	[[Category: Ligand specificity]]
		+	[[Category: Manganese]]
		+	[[Category: Metal-binding]]
		+	[[Category: Methylation]]
		+	[[Category: Nucleus]]
		+	[[Category: Phosphatase]]
		+	[[Category: Phosphoprotein]]
		+	[[Category: Polymorphism]]
		+	[[Category: Protein phosphatase]]
		+	[[Category: Protein-protein complex]]
		+	[[Category: Signaling protein]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Nov 4 11:04:42 2009''

OCA: New page: '''Unreleased structure''' The entry 3k7v is ON HOLD Authors: Jeffrey, P.D., Huhn, J., Shi, Y. Description: Protein phosphatase 2A core complex bound to dinophysistoxin-1 ''Page seede...

OCA — Wed, 21 Oct 2009 08:01:28 GMT

New page: '''Unreleased structure''' The entry 3k7v is ON HOLD Authors: Jeffrey, P.D., Huhn, J., Shi, Y. Description: Protein phosphatase 2A core complex bound to dinophysistoxin-1 ''Page seede...

New page

'''Unreleased structure'''

The entry 3k7v is ON HOLD

Authors: Jeffrey, P.D., Huhn, J., Shi, Y.

Description: Protein phosphatase 2A core complex bound to dinophysistoxin-1

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct 21 10:01:27 2009''