3k7z - Revision history

OCA at 10:15, 21 February 2024

OCA — Wed, 21 Feb 2024 10:15:34 GMT

←Older revision		Revision as of 10:15, 21 February 2024
Line 9:		Line 9:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/GCN4_YEAST GCN4_YEAST] Is a transcription factor that is responsible for the activation of more than 30 genes required for amino acid or for purine biosynthesis in response to amino acid or purine starvation. Binds and recognize the DNA sequence: 5'-TGA[CG]TCA-3'.		[https://www.uniprot.org/uniprot/GCN4_YEAST GCN4_YEAST] Is a transcription factor that is responsible for the activation of more than 30 genes required for amino acid or for purine biosynthesis in response to amino acid or purine starvation. Binds and recognize the DNA sequence: 5'-TGA[CG]TCA-3'.
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Efficient determination of protein crystal structures requires automated x-ray data analysis. Here, we describe the expert system ELVES and its use to determine automatically the structure of a 12-kDa protein. Multiwavelength anomalous diffraction analysis of a selenomethionyl derivative was used to image the Asn-16-Ala variant of the GCN4 leucine zipper. In contrast to the parallel, dimeric coiled coil formed by the WT sequence, the mutant unexpectedly formed an antiparallel trimer. This structural switch reveals how avoidance of core cavities at a single site can select the native fold of a protein. All structure calculations, including indexing, data processing, locating heavy atoms, phasing by multiwavelength anomalous diffraction, model building, and refinement, were completed without human intervention. The results demonstrate the feasibility of automated methods for determining high-resolution, x-ray crystal structures of proteins.
-
-	Automated protein crystal structure determination using ELVES.,Holton J, Alber T Proc Natl Acad Sci U S A. 2004 Feb 10;101(6):1537-42. Epub 2004 Jan 29. PMID:14752198<ref>PMID:14752198</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3k7z" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Gcn4 3D Structures\|Gcn4 3D Structures]]		*[[Gcn4 3D Structures\|Gcn4 3D Structures]]
	*[[Gnc4 3D Structures\|Gnc4 3D Structures]]		*[[Gnc4 3D Structures\|Gnc4 3D Structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 06:34, 19 July 2023

OCA — Wed, 19 Jul 2023 06:34:28 GMT

←Older revision		Revision as of 06:34, 19 July 2023
Line 3:		Line 3:
	<StructureSection load='3k7z' size='340' side='right'caption='[[3k7z]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='3k7z' size='340' side='right'caption='[[3k7z]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3k7z]] is a 3 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1rb1 1rb1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K7Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3K7Z FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3k7z]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1rb1 1rb1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K7Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3K7Z FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~<div style='overflow: auto; max~~-~~height: 3em;'>[[1rb4\|1rb4]]~~, [[~~1rb5~~\|~~1rb5~~]]~~, [[1rb6\|1rb6]]</div>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3k7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k7z OCA], [https://pdbe.org/3k7z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3k7z RCSB], [https://www.ebi.ac.uk/pdbsum/3k7z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3k7z ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3k7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k7z OCA], [https://pdbe.org/3k7z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3k7z RCSB], [https://www.ebi.ac.uk/pdbsum/3k7z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3k7z ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/GCN4_YEAST GCN4_YEAST]] Is a transcription factor that is responsible for the activation of more than 30 genes required for amino acid or for purine biosynthesis in response to amino acid or purine starvation. Binds and recognize the DNA sequence: 5'-TGA[CG]TCA-3'.	+	[https://www.uniprot.org/uniprot/GCN4_YEAST GCN4_YEAST] Is a transcription factor that is responsible for the activation of more than 30 genes required for amino acid or for purine biosynthesis in response to amino acid or purine starvation. Binds and recognize the DNA sequence: 5'-TGA[CG]TCA-3'.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 20:

	==See Also==		==See Also==
		+	*[[Gcn4 3D Structures\|Gcn4 3D Structures]]
	*[[Gnc4 3D Structures\|Gnc4 3D Structures]]		*[[Gnc4 3D Structures\|Gnc4 3D Structures]]
	== References ==		== References ==
Line 26:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Alber, T~~]]	+	[[Category: Saccharomyces cerevisiae S288C]]
-	[[Category: ~~Holton, J~~]]	+	[[Category: Alber T]]
-	[[Category: ~~Structural genomic]]~~	+	[[Category: Holton J]]
-	~~[[Category: Activator]]~~	+
-	~~[[Category: Amino-acid biosynthesis]]~~	+
-	~~[[Category: Coiled coil]]~~	+
-	~~[[Category: Dna binding protein]]~~	+
-	~~[[Category: Dna-binding]]~~	+
-	~~[[Category: Leucine zipper]]~~	+
-	~~[[Category: Nucleus]]~~	+
-	~~[[Category: Phosphoprotein]]~~	+
-	~~[[Category: Tbsgc]]~~	+
-	~~[[Category: Transcription]]~~	+
-	~~[[Category: Transcription regulation~~]]	+

OCA at 12:22, 13 October 2021

OCA — Wed, 13 Oct 2021 12:22:22 GMT

←Older revision		Revision as of 12:22, 13 October 2021
Line 3:		Line 3:
	<StructureSection load='3k7z' size='340' side='right'caption='[[3k7z]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='3k7z' size='340' side='right'caption='[[3k7z]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3k7z]] is a 3 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1rb1 1rb1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K7Z OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=3K7Z FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3k7z]] is a 3 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1rb1 1rb1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K7Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3K7Z FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1rb4\|1rb4]], [[1rb5\|1rb5]], [[1rb6\|1rb6]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1rb4\|1rb4]], [[1rb5\|1rb5]], [[1rb6\|1rb6]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=3k7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k7z OCA], [~~http~~://pdbe.org/3k7z PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3k7z RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3k7z PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3k7z ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3k7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k7z OCA], [https://pdbe.org/3k7z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3k7z RCSB], [https://www.ebi.ac.uk/pdbsum/3k7z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3k7z ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/GCN4_YEAST GCN4_YEAST]] Is a transcription factor that is responsible for the activation of more than 30 genes required for amino acid or for purine biosynthesis in response to amino acid or purine starvation. Binds and recognize the DNA sequence: 5'-TGA[CG]TCA-3'.	+	[[https://www.uniprot.org/uniprot/GCN4_YEAST GCN4_YEAST]] Is a transcription factor that is responsible for the activation of more than 30 genes required for amino acid or for purine biosynthesis in response to amino acid or purine starvation. Binds and recognize the DNA sequence: 5'-TGA[CG]TCA-3'.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 07:00, 7 October 2020

OCA — Wed, 07 Oct 2020 07:00:32 GMT

←Older revision		Revision as of 07:00, 7 October 2020
Line 1:		Line 1:

	==GCN4-Leucine zipper core mutant as N16A trigonal automatic solution==		==GCN4-Leucine zipper core mutant as N16A trigonal automatic solution==
-	<StructureSection load='3k7z' size='340' side='right' caption='[[3k7z]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='3k7z' size='340' side='right'caption='[[3k7z]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3k7z]] is a 3 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1rb1 1rb1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K7Z OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3K7Z FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3k7z]] is a 3 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1rb1 1rb1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K7Z OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3K7Z FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1rb4\|1rb4]], [[1rb5\|1rb5]], [[1rb6\|1rb6]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1rb4\|1rb4]], [[1rb5\|1rb5]], [[1rb6\|1rb6]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3k7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k7z OCA], [http://pdbe.org/3k7z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3k7z RCSB], [http://www.ebi.ac.uk/pdbsum/3k7z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3k7z ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3k7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k7z OCA], [http://pdbe.org/3k7z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3k7z RCSB], [http://www.ebi.ac.uk/pdbsum/3k7z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3k7z ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 20:		Line 20:

	==See Also==		==See Also==
-	*[[~~Gcn4~~\|~~Gcn4~~]]	+	*[[Gnc4 3D Structures\|Gnc4 3D Structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Alber, T]]		[[Category: Alber, T]]
	[[Category: Holton, J]]		[[Category: Holton, J]]

OCA at 08:55, 26 December 2018

OCA — Wed, 26 Dec 2018 08:55:58 GMT

←Older revision		Revision as of 08:55, 26 December 2018
Line 30:		Line 30:
	[[Category: Activator]]		[[Category: Activator]]
	[[Category: Amino-acid biosynthesis]]		[[Category: Amino-acid biosynthesis]]
		+	[[Category: Coiled coil]]
	[[Category: Dna binding protein]]		[[Category: Dna binding protein]]
	[[Category: Dna-binding]]		[[Category: Dna-binding]]

OCA at 09:16, 5 August 2016

OCA — Fri, 05 Aug 2016 09:16:05 GMT

←Older revision		Revision as of 09:16, 5 August 2016
Line 1:		Line 1:
		+
	==GCN4-Leucine zipper core mutant as N16A trigonal automatic solution==		==GCN4-Leucine zipper core mutant as N16A trigonal automatic solution==
	<StructureSection load='3k7z' size='340' side='right' caption='[[3k7z]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='3k7z' size='340' side='right' caption='[[3k7z]], [[Resolution\|resolution]] 1.90Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[3k7z]] is a 3 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1rb1 1rb1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K7Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3K7Z FirstGlance]. <br>		<table><tr><td colspan='2'>[[3k7z]] is a 3 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1rb1 1rb1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K7Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3K7Z FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1rb4\|1rb4]], [[1rb5\|1rb5]], [[1rb6\|1rb6]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1rb4\|1rb4]], [[1rb5\|1rb5]], [[1rb6\|1rb6]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3k7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k7z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3k7z RCSB], [http://www.ebi.ac.uk/pdbsum/3k7z PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3k7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k7z OCA], [http://pdbe.org/3k7z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3k7z RCSB], [http://www.ebi.ac.uk/pdbsum/3k7z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3k7z ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3k7z" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 01:05, 25 December 2014

OCA — Thu, 25 Dec 2014 01:05:55 GMT

←Older revision		Revision as of 01:05, 25 December 2014
Line 6:		Line 6:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3k7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k7z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3k7z RCSB], [http://www.ebi.ac.uk/pdbsum/3k7z PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3k7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k7z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3k7z RCSB], [http://www.ebi.ac.uk/pdbsum/3k7z PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/GCN4_YEAST GCN4_YEAST]] Is a transcription factor that is responsible for the activation of more than 30 genes required for amino acid or for purine biosynthesis in response to amino acid or purine starvation. Binds and recognize the DNA sequence: 5'-TGA[CG]TCA-3'.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 15:47, 18 December 2014

OCA — Thu, 18 Dec 2014 15:47:47 GMT

←Older revision		Revision as of 15:47, 18 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3k7z\| PDB=3k7z \| SCENE= }}~~	+	==GCN4-Leucine zipper core mutant as N16A trigonal automatic solution==
-	===GCN4-Leucine zipper core mutant as N16A trigonal automatic solution===	+	<StructureSection load='3k7z' size='340' side='right' caption='[[3k7z]], [[Resolution\|resolution]] 1.90Å' scene=''>
-	~~{{ABSTRACT_PUBMED_14752198}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3k7z]] is a 3 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1rb1 1rb1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K7Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3K7Z FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1rb4\|1rb4]], [[1rb5\|1rb5]], [[1rb6\|1rb6]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3k7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k7z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3k7z RCSB], [http://www.ebi.ac.uk/pdbsum/3k7z PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Efficient determination of protein crystal structures requires automated x-ray data analysis. Here, we describe the expert system ELVES and its use to determine automatically the structure of a 12-kDa protein. Multiwavelength anomalous diffraction analysis of a selenomethionyl derivative was used to image the Asn-16-Ala variant of the GCN4 leucine zipper. In contrast to the parallel, dimeric coiled coil formed by the WT sequence, the mutant unexpectedly formed an antiparallel trimer. This structural switch reveals how avoidance of core cavities at a single site can select the native fold of a protein. All structure calculations, including indexing, data processing, locating heavy atoms, phasing by multiwavelength anomalous diffraction, model building, and refinement, were completed without human intervention. The results demonstrate the feasibility of automated methods for determining high-resolution, x-ray crystal structures of proteins.

-	~~==Function==~~	+	Automated protein crystal structure determination using ELVES.,Holton J, Alber T Proc Natl Acad Sci U S A. 2004 Feb 10;101(6):1537-42. Epub 2004 Jan 29. PMID:14752198<ref>PMID:14752198</ref>
-	~~[[http://www~~.~~uniprot~~.org/uniprot/GCN4_YEAST GCN4_YEAST]] Is a transcription factor that is responsible for the activation of more than 30 genes required for amino acid or for purine biosynthesis in response to amino acid or purine starvation. Binds and recognize the DNA sequence: 5'-~~TGA[CG]TCA-3'~~.	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[3k7z]] is~~ a ~~3 chain structure. This structure supersedes~~ the ~~now removed PDB entry [http://oca~~.~~weizmann~~.ac.il/~~oca-bin/send-pdb?obs=1&id=1rb1 1rb1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K7Z OCA].~~	+	</div>

-	==~~Reference~~==	+	==See Also==
-	~~<ref group~~=~~"xtra">PMID:014752198</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	*[[Gcn4\|Gcn4]]
-	[[Category: Alber, T.]]	+	== References ==
-	[[Category: Holton, J.]]	+	<references/>
-	[[Category: ~~TBSGC, TB~~ Structural ~~Genomics Consortium.~~]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Alber, T]]
		+	[[Category: Holton, J]]
		+	[[Category: Structural genomic]]
	[[Category: Activator]]		[[Category: Activator]]
	[[Category: Amino-acid biosynthesis]]		[[Category: Amino-acid biosynthesis]]
Line 21:		Line 31:
	[[Category: Nucleus]]		[[Category: Nucleus]]
	[[Category: Phosphoprotein]]		[[Category: Phosphoprotein]]
-	[[Category: Structural genomic]]
-	[[Category: Tb structural genomics consortium]]
	[[Category: Tbsgc]]		[[Category: Tbsgc]]
	[[Category: Transcription]]		[[Category: Transcription]]
	[[Category: Transcription regulation]]		[[Category: Transcription regulation]]

OCA: Protected "3k7z" [edit=sysop:move=sysop]

OCA — Thu, 04 Apr 2013 02:07:44 GMT

Protected "3k7z" [edit=sysop:move=sysop]

←Older revision

Revision as of 02:07, 4 April 2013

OCA at 02:07, 4 April 2013

OCA — Thu, 04 Apr 2013 02:07:35 GMT

←Older revision		Revision as of 02:07, 4 April 2013
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-	{{Seed}}
-	[[Image:3k7z.jpg\|left\|200px]]
-
-	<!--
-	The line below this paragraph, containing "STRUCTURE_3k7z", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3k7z\| PDB=3k7z \| SCENE= }}		{{STRUCTURE_3k7z\| PDB=3k7z \| SCENE= }}
-
	===GCN4-Leucine zipper core mutant as N16A trigonal automatic solution===		===GCN4-Leucine zipper core mutant as N16A trigonal automatic solution===
		+	{{ABSTRACT_PUBMED_14752198}}

-		+	==Function==
-	~~<!--~~	+	[[http://www.uniprot.org/uniprot/GCN4_YEAST GCN4_YEAST]] Is a transcription factor that is responsible for the activation of more than 30 genes required for amino acid or for purine biosynthesis in response to amino acid or purine starvation. Binds and recognize the DNA sequence: 5'-TGA[CG]TCA-3'.
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_14752198}}, adds the Publication Abstract to the page~~	+
-	~~(as it appears on PubMed at~~ http://www.~~pubmed~~.~~gov), where 14752198~~ is the ~~PubMed ID number~~.	+
-	-->	+
-	~~{{ABSTRACT_PUBMED_14752198}}~~	+

	==About this Structure==		==About this Structure==
-	~~3K7Z~~ is a 3 ~~chains~~ structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1rb1 1rb1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K7Z OCA].	+	[[3k7z]] is a 3 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1rb1 1rb1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K7Z OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~14752198~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:014752198</ref><references group="xtra"/><references/>
	[[Category: Alber, T.]]		[[Category: Alber, T.]]
	[[Category: Holton, J.]]		[[Category: Holton, J.]]
Line 29:		Line 16:
	[[Category: Activator]]		[[Category: Activator]]
	[[Category: Amino-acid biosynthesis]]		[[Category: Amino-acid biosynthesis]]
-	[[Category: ~~Coiled coil~~]]	+	[[Category: Dna binding protein]]
	[[Category: Dna-binding]]		[[Category: Dna-binding]]
	[[Category: Leucine zipper]]		[[Category: Leucine zipper]]
	[[Category: Nucleus]]		[[Category: Nucleus]]
-	[[Category: Peptide]]
	[[Category: Phosphoprotein]]		[[Category: Phosphoprotein]]
	[[Category: Structural genomic]]		[[Category: Structural genomic]]
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	[[Category: Transcription]]		[[Category: Transcription]]
	[[Category: Transcription regulation]]		[[Category: Transcription regulation]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Nov 25 09:23:02 2009''