3kjo - Revision history

OCA at 08:17, 6 September 2023

OCA — Wed, 06 Sep 2023 08:17:08 GMT

←Older revision		Revision as of 08:17, 6 September 2023
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	==Crystal Structure of hPOT1V2-dTrUd(AGGGTTAG)==		==Crystal Structure of hPOT1V2-dTrUd(AGGGTTAG)==
-	<StructureSection load='3kjo' size='340' side='right' caption='[[3kjo]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='3kjo' size='340' side='right'caption='[[3kjo]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3kjo]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KJO OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3KJO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3kjo]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KJO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3KJO FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~>[[1xjv\|1xjv]], [[3kjp\|3kjp]]</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">POT1 ([http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=9606 HUMAN])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3kjo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kjo OCA], [https://pdbe.org/3kjo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3kjo RCSB], [https://www.ebi.ac.uk/pdbsum/3kjo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3kjo ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3kjo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kjo OCA], [~~http~~://pdbe.org/3kjo PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3kjo RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3kjo PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3kjo ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/POTE1_HUMAN POTE1_HUMAN]] Component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. Is a component of the double-stranded telomeric DNA-binding TRF1 complex which is involved in the regulation of telomere length by cis-inhibition of telomerase. Also acts as a single-stranded telomeric DNA-binding protein and thus may act as a downstream effector of the TRF1 complex and may transduce information about telomere maintenance and/or length to the telomere terminus. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Binds to two or more telomeric single-stranded 5'-TTAGGG-3' repeats (G-strand) and with high specificity to a minimal telomeric single-stranded 5'-TAGGGTTAG-3' sequence. Binds telomeric single-stranded sequences internally or at proximity of a 3'-end. Its activity is TERT dependent but it does not increase TERT activity by itself. In contrast, the ACD-POT1 heterodimer enhances telomere elongation by increasing telomerase processivity.<ref>PMID:12781132</ref> <ref>PMID:12768206</ref> <ref>PMID:16166375</ref> <ref>PMID:17237768</ref> <ref>PMID:20231318</ref>	+	[https://www.uniprot.org/uniprot/POTE1_HUMAN POTE1_HUMAN] Component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. Is a component of the double-stranded telomeric DNA-binding TRF1 complex which is involved in the regulation of telomere length by cis-inhibition of telomerase. Also acts as a single-stranded telomeric DNA-binding protein and thus may act as a downstream effector of the TRF1 complex and may transduce information about telomere maintenance and/or length to the telomere terminus. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Binds to two or more telomeric single-stranded 5'-TTAGGG-3' repeats (G-strand) and with high specificity to a minimal telomeric single-stranded 5'-TAGGGTTAG-3' sequence. Binds telomeric single-stranded sequences internally or at proximity of a 3'-end. Its activity is TERT dependent but it does not increase TERT activity by itself. In contrast, the ACD-POT1 heterodimer enhances telomere elongation by increasing telomerase processivity.<ref>PMID:12781132</ref> <ref>PMID:12768206</ref> <ref>PMID:16166375</ref> <ref>PMID:17237768</ref> <ref>PMID:20231318</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kj/3kjo_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kj/3kjo_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 33:		Line 32:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
-	[[Category: ~~Cech, T R~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Nandakumar, J~~]]	+	[[Category: Cech TR]]
-	[[Category: ~~Podell, E R~~]]	+	[[Category: Nandakumar J]]
-	[[Category: ~~Alternative splicing]]~~	+	[[Category: Podell ER]]
-	~~[[Category: Chromosomal protein]]~~	+
-	~~[[Category: Dna binding protein-dna complex]]~~	+
-	~~[[Category: Dna-binding]]~~	+
-	~~[[Category: Nucleus]]~~	+
-	~~[[Category: Ob domain]]~~	+
-	~~[[Category: Polymorphism]]~~	+
-	~~[[Category: Protein-dna complex]]~~	+
-	~~[[Category: Protein-rna complex]]~~	+
-	~~[[Category: Telomere~~]]	+

OCA at 08:35, 1 November 2017

OCA — Wed, 01 Nov 2017 08:35:07 GMT

←Older revision		Revision as of 08:35, 1 November 2017
Line 1:		Line 1:
		+
	==Crystal Structure of hPOT1V2-dTrUd(AGGGTTAG)==		==Crystal Structure of hPOT1V2-dTrUd(AGGGTTAG)==
	<StructureSection load='3kjo' size='340' side='right' caption='[[3kjo]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='3kjo' size='340' side='right' caption='[[3kjo]], [[Resolution\|resolution]] 1.80Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1xjv\|1xjv]], [[3kjp\|3kjp]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1xjv\|1xjv]], [[3kjp\|3kjp]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">POT1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">POT1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3kjo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kjo OCA], [http://pdbe.org/3kjo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3kjo RCSB], [http://www.ebi.ac.uk/pdbsum/3kjo PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3kjo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kjo OCA], [http://pdbe.org/3kjo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3kjo RCSB], [http://www.ebi.ac.uk/pdbsum/3kjo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3kjo ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 36:		Line 37:
	[[Category: Nandakumar, J]]		[[Category: Nandakumar, J]]
	[[Category: Podell, E R]]		[[Category: Podell, E R]]
		+	[[Category: Alternative splicing]]
	[[Category: Chromosomal protein]]		[[Category: Chromosomal protein]]
	[[Category: Dna binding protein-dna complex]]		[[Category: Dna binding protein-dna complex]]
Line 41:		Line 43:
	[[Category: Nucleus]]		[[Category: Nucleus]]
	[[Category: Ob domain]]		[[Category: Ob domain]]
		+	[[Category: Polymorphism]]
	[[Category: Protein-dna complex]]		[[Category: Protein-dna complex]]
	[[Category: Protein-rna complex]]		[[Category: Protein-rna complex]]
	[[Category: Telomere]]		[[Category: Telomere]]

OCA at 07:05, 10 February 2016

OCA — Wed, 10 Feb 2016 07:05:09 GMT

←Older revision		Revision as of 07:05, 10 February 2016
Line 2:		Line 2:
	<StructureSection load='3kjo' size='340' side='right' caption='[[3kjo]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='3kjo' size='340' side='right' caption='[[3kjo]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3kjo]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KJO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3KJO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3kjo]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KJO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3KJO FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1xjv\|1xjv]], [[3kjp\|3kjp]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1xjv\|1xjv]], [[3kjp\|3kjp]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">POT1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">POT1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3kjo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kjo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3kjo RCSB], [http://www.ebi.ac.uk/pdbsum/3kjo PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3kjo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kjo OCA], [http://pdbe.org/3kjo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3kjo RCSB], [http://www.ebi.ac.uk/pdbsum/3kjo PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3kjo ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3kjo" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Cech, T R]]		[[Category: Cech, T R]]
	[[Category: Nandakumar, J]]		[[Category: Nandakumar, J]]

OCA at 12:37, 25 December 2014

OCA — Thu, 25 Dec 2014 12:37:34 GMT

←Older revision		Revision as of 12:37, 25 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3kjo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kjo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3kjo RCSB], [http://www.ebi.ac.uk/pdbsum/3kjo PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3kjo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kjo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3kjo RCSB], [http://www.ebi.ac.uk/pdbsum/3kjo PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/POTE1_HUMAN POTE1_HUMAN]] Component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. Is a component of the double-stranded telomeric DNA-binding TRF1 complex which is involved in the regulation of telomere length by cis-inhibition of telomerase. Also acts as a single-stranded telomeric DNA-binding protein and thus may act as a downstream effector of the TRF1 complex and may transduce information about telomere maintenance and/or length to the telomere terminus. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Binds to two or more telomeric single-stranded 5'-TTAGGG-3' repeats (G-strand) and with high specificity to a minimal telomeric single-stranded 5'-TAGGGTTAG-3' sequence. Binds telomeric single-stranded sequences internally or at proximity of a 3'-end. Its activity is TERT dependent but it does not increase TERT activity by itself. In contrast, the ACD-POT1 heterodimer enhances telomere elongation by increasing telomerase processivity.<ref>PMID:12781132</ref> <ref>PMID:12768206</ref> <ref>PMID:16166375</ref> <ref>PMID:17237768</ref> <ref>PMID:20231318</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 17:16, 18 December 2014

OCA — Thu, 18 Dec 2014 17:16:11 GMT

←Older revision		Revision as of 17:16, 18 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3kjo\| PDB=3kjo \| SCENE= }}~~	+	==Crystal Structure of hPOT1V2-dTrUd(AGGGTTAG)==
-	===Crystal Structure of hPOT1V2-dTrUd(AGGGTTAG)===	+	<StructureSection load='3kjo' size='340' side='right' caption='[[3kjo]], [[Resolution\|resolution]] 1.80Å' scene=''>
-	~~{{ABSTRACT_PUBMED_20080730}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3kjo]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KJO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3KJO FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1xjv\|1xjv]], [[3kjp\|3kjp]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">POT1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3kjo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kjo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3kjo RCSB], [http://www.ebi.ac.uk/pdbsum/3kjo PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kj/3kjo_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The POT1-TPP1 heterodimer, the major telomere-specific single-stranded DNA-binding protein in mammalian cells, protects chromosome ends and contributes to the regulation of telomerase. The recent discovery of telomeric RNA raises the question of how POT1 faithfully binds telomeric ssDNA and avoids illicit RNA binding that could result in its depletion from telomeres. Here we show through binding studies that a single deoxythymidine in a telomeric repeat dictates the DNA versus RNA discrimination by human POT1 and mouse POT1A. We solve the crystal structure of hPOT1 bound to DNA with a ribouridine in lieu of the critical deoxythymidine and show that this substitution results in burying the 2(')-hydroxyl group in a hydrophobic region (Phe62) of POT1 in addition to eliminating favorable hydrogen-bonding interactions at the POT1-nucleic acid interface. At amino acid 62, Phe discriminates against RNA binding and Tyr allows RNA binding. We further show that TPP1 greatly augments POT1's discrimination against RNA.

-	~~==Function==~~	+	How telomeric protein POT1 avoids RNA to achieve specificity for single-stranded DNA.,Nandakumar J, Podell ER, Cech TR Proc Natl Acad Sci U S A. 2010 Jan 12;107(2):651-656. Epub 2009 Dec 22. PMID:20080730<ref>PMID:20080730</ref>
-	~~[[http://www.uniprot.org/uniprot/POTE1_HUMAN POTE1_HUMAN]] Component of the telomerase ribonucleoprotein (RNP) complex that is essential~~ for the replication of chromosome termini. Is a component of the double-stranded telomeric DNA-binding TRF1 complex which is involved in the regulation of telomere length by cis-inhibition of telomerase. Also acts as a single-stranded ~~telomeric~~ DNA~~-binding protein and thus may act as a downstream effector of the TRF1 complex and may transduce information about telomere maintenance and/or length to the telomere terminus~~. ~~Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection~~. ~~Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends~~; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Binds to two or more telomeric single-stranded 5'-TTAGGG-3' repeats (~~G-strand~~) ~~and with high specificity to a minimal telomeric single-stranded 5'-TAGGGTTAG~~-~~3' sequence~~. ~~Binds telomeric single-stranded sequences internally or at proximity of a 3'-end~~. ~~Its activity is TERT dependent but it does not increase TERT activity by itself. In contrast, the ACD-POT1 heterodimer enhances telomere elongation by increasing telomerase processivity.<ref>~~PMID:~~12781132</ref> <ref>PMID:12768206</ref> <ref>PMID:16166375</ref> <ref>PMID:17237768</ref>~~ <ref>PMID:~~20231318~~</ref>	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[3kjo]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3KJO OCA].~~	+	</div>
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:020080730</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Cech, T R.]]	+	[[Category: Cech, T R]]
-	[[Category: Nandakumar, J.]]	+	[[Category: Nandakumar, J]]
-	[[Category: Podell, E R.]]	+	[[Category: Podell, E R]]
	[[Category: Chromosomal protein]]		[[Category: Chromosomal protein]]
	[[Category: Dna binding protein-dna complex]]		[[Category: Dna binding protein-dna complex]]

OCA: Protected "3kjo" [edit=sysop:move=sysop]

OCA — Thu, 04 Apr 2013 02:38:34 GMT

Protected "3kjo" [edit=sysop:move=sysop]

←Older revision

Revision as of 02:38, 4 April 2013

OCA at 02:38, 4 April 2013

OCA — Thu, 04 Apr 2013 02:38:33 GMT

←Older revision		Revision as of 02:38, 4 April 2013
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-	{{Seed}}
-	[[Image:3kjo.png\|left\|200px]]
-
-	<!--
-	The line below this paragraph, containing "STRUCTURE_3kjo", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3kjo\| PDB=3kjo \| SCENE= }}		{{STRUCTURE_3kjo\| PDB=3kjo \| SCENE= }}
-
	===Crystal Structure of hPOT1V2-dTrUd(AGGGTTAG)===		===Crystal Structure of hPOT1V2-dTrUd(AGGGTTAG)===
		+	{{ABSTRACT_PUBMED_20080730}}

-		+	==Function==
-	~~<!--~~	+	[[http://www.uniprot.org/uniprot/POTE1_HUMAN POTE1_HUMAN]] Component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. Is a component of the double-stranded telomeric DNA-binding TRF1 complex which is involved in the regulation of telomere length by cis-inhibition of telomerase. Also acts as a single-stranded telomeric DNA-binding protein and thus may act as a downstream effector of the TRF1 complex and may transduce information about telomere maintenance and/or length to the telomere terminus. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Binds to two or more telomeric single-stranded 5'-TTAGGG-3' repeats (G-strand) and with high specificity to a minimal telomeric single-stranded 5'-TAGGGTTAG-3' sequence. Binds telomeric single-stranded sequences internally or at proximity of a 3'-end. Its activity is TERT dependent but it does not increase TERT activity by itself. In contrast, the ACD-POT1 heterodimer enhances telomere elongation by increasing telomerase processivity.<ref>PMID:12781132</ref> <ref>PMID:12768206</ref> <ref>PMID:16166375</ref> <ref>PMID:17237768</ref> <ref>PMID:20231318</ref>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_20080730}}, adds the Publication Abstract to the page~~	+
-	~~(as it appears on PubMed at~~ http://www.~~pubmed~~.~~gov~~)~~, where 20080730~~ is the ~~PubMed ID number~~.	+
-	-->	+
-	~~{{ABSTRACT_PUBMED_20080730}}~~	+

	==About this Structure==		==About this Structure==
-	~~3KJO~~ is a 2 ~~chains~~ structure with ~~sequences~~ from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KJO OCA].	+	[[3kjo]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KJO OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~20080730~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:020080730</ref><references group="xtra"/><references/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Cech, T R.]]		[[Category: Cech, T R.]]
	[[Category: Nandakumar, J.]]		[[Category: Nandakumar, J.]]
	[[Category: Podell, E R.]]		[[Category: Podell, E R.]]
-	[[Category: Alternative splicing]]
	[[Category: Chromosomal protein]]		[[Category: Chromosomal protein]]
		+	[[Category: Dna binding protein-dna complex]]
	[[Category: Dna-binding]]		[[Category: Dna-binding]]
	[[Category: Nucleus]]		[[Category: Nucleus]]
	[[Category: Ob domain]]		[[Category: Ob domain]]
-	[[Category: Polymorphism]]
	[[Category: Protein-dna complex]]		[[Category: Protein-dna complex]]
	[[Category: Protein-rna complex]]		[[Category: Protein-rna complex]]
	[[Category: Telomere]]		[[Category: Telomere]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 3 09:02:07 2010''

OCA at 07:02, 3 February 2010

OCA — Wed, 03 Feb 2010 07:02:07 GMT

←Older revision		Revision as of 07:02, 3 February 2010
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	{{Seed}}		{{Seed}}
-	[[Image:3kjo.~~jpg~~\|left\|200px]]	+	[[Image:3kjo.png\|left\|200px]]

	<!--		<!--
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	===Crystal Structure of hPOT1V2-dTrUd(AGGGTTAG)===		===Crystal Structure of hPOT1V2-dTrUd(AGGGTTAG)===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_20080730}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 20080730 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_20080730}}

	==About this Structure==		==About this Structure==
	3KJO is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KJO OCA].		3KJO is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KJO OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:20080730</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Cech, T R.]]		[[Category: Cech, T R.]]
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	[[Category: Telomere]]		[[Category: Telomere]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Jan 20 16~~:00:46 2010''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 3 09:02:07 2010''

OCA at 14:00, 20 January 2010

OCA — Wed, 20 Jan 2010 14:00:47 GMT

←Older revision		Revision as of 14:00, 20 January 2010
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-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3kjo.jpg\|left\|200px]]

-	The ~~entry 3kjo~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3kjo", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3kjo\| PDB=3kjo \| SCENE= }}

-	~~Authors: Nandakumar, J., Cech, T.R., Podell, E.R.~~	+	===Crystal Structure of hPOT1V2-dTrUd(AGGGTTAG)===

-	Description: Crystal Structure of hPOT1V2-dTrUd(AGGGTTAG)

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Nov 25 09~~:13:~~02 2009~~''	+	==About this Structure==
		+	3KJO is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KJO OCA].
		+	[[Category: Homo sapiens]]
		+	[[Category: Cech, T R.]]
		+	[[Category: Nandakumar, J.]]
		+	[[Category: Podell, E R.]]
		+	[[Category: Alternative splicing]]
		+	[[Category: Chromosomal protein]]
		+	[[Category: Dna-binding]]
		+	[[Category: Nucleus]]
		+	[[Category: Ob domain]]
		+	[[Category: Polymorphism]]
		+	[[Category: Protein-dna complex]]
		+	[[Category: Protein-rna complex]]
		+	[[Category: Telomere]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 20 16:00:46 2010''

OCA at 07:13, 25 November 2009

OCA — Wed, 25 Nov 2009 07:13:02 GMT

←Older revision		Revision as of 07:13, 25 November 2009
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3kjo is ON HOLD	+	The entry 3kjo is ON HOLD until Paper Publication

	Authors: Nandakumar, J., Cech, T.R., Podell, E.R.		Authors: Nandakumar, J., Cech, T.R., Podell, E.R.
Line 7:		Line 7:
	Description: Crystal Structure of hPOT1V2-dTrUd(AGGGTTAG)		Description: Crystal Structure of hPOT1V2-dTrUd(AGGGTTAG)

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Nov ~~18 18~~:57:26 2009''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Nov 25 09:13:02 2009''