3krf - Revision history

OCA at 08:30, 20 March 2024

OCA — Wed, 20 Mar 2024 08:30:10 GMT

←Older revision		Revision as of 08:30, 20 March 2024
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	<StructureSection load='3krf' size='340' side='right'caption='[[3krf]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='3krf' size='340' side='right'caption='[[3krf]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3krf]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Menpi Menpi~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KRF OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3KRF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3krf]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mentha_x_piperita Mentha x piperita]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KRF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3KRF FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DST:DIMETHYLALLYL+S-THIOLODIPHOSPHATE'>DST</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=IPE:3-METHYLBUT-3-ENYL+TRIHYDROGEN+DIPHOSPHATE'>IPE</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PPV:PYROPHOSPHATE'>PPV</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3kra\|3kra]], [[3krc\|3krc]], [[3kro\|3kro]], [[3krp\|3krp]]</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DST:DIMETHYLALLYL+S-THIOLODIPHOSPHATE'>DST</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=IPE:3-METHYLBUT-3-ENYL+TRIHYDROGEN+DIPHOSPHATE'>IPE</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PPV:PYROPHOSPHATE'>PPV</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GPPS Large Subunit ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=34256 MENPI]), GPPS Small subunit ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=34256 MENPI])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3krf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3krf OCA], [https://pdbe.org/3krf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3krf RCSB], [https://www.ebi.ac.uk/pdbsum/3krf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3krf ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dimethylallyltranstransferase Dimethylallyltranstransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.1 2.5.1.1] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3krf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3krf OCA], [~~http~~://pdbe.org/3krf PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3krf RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3krf PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3krf ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q9SBR3_MENPI Q9SBR3_MENPI]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3krf ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3krf ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Terpenes (isoprenoids), derived from isoprenyl pyrophosphates, are versatile natural compounds that act as metabolism mediators, plant volatiles, and ecological communicators. Divergent evolution of homomeric prenyltransferases (PTSs) has allowed PTSs to optimize their active-site pockets to achieve catalytic fidelity and diversity. Little is known about heteromeric PTSs, particularly the mechanisms regulating formation of specific products. Here, we report the crystal structure of the (LSU . SSU)(2)-type (LSU/SSU = large/small subunit) heterotetrameric geranyl pyrophosphate synthase (GPPS) from mint (Mentha piperita). The LSU and SSU of mint GPPS are responsible for catalysis and regulation, respectively, and this SSU lacks the essential catalytic amino acid residues found in LSU and other PTSs. Whereas no activity was detected for individually expressed LSU or SSU, the intact (LSU . SSU)(2) tetramer produced not only C(10)-GPP at the beginning of the reaction but also C(20)-GGPP (geranylgeranyl pyrophosphate) at longer reaction times. The activity for synthesizing C(10)-GPP and C(20)-GGPP, but not C(15)-farnesyl pyrophosphate, reflects a conserved active-site structure of the LSU and the closely related mustard (Sinapis alba) homodimeric GGPPS. Furthermore, using a genetic complementation system, we showed that no C(20)-GGPP is produced by the mint GPPS in vivo. Presumably through protein-protein interactions, the SSU remodels the active-site cavity of LSU for synthesizing C(10)-GPP, the precursor of volatile C(10)-monoterpenes.
-
-	Structure of a heterotetrameric geranyl pyrophosphate synthase from mint (Mentha piperita) reveals intersubunit regulation.,Chang TH, Hsieh FL, Ko TP, Teng KH, Liang PH, Wang AH Plant Cell. 2010 Feb;22(2):454-67. Epub 2010 Feb 5. PMID:20139160<ref>PMID:20139160</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3krf" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Dimethylallyltranstransferase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Menpi~~]]	+	[[Category: Mentha x piperita]]
-	[[Category: Chang, T H]]	+	[[Category: Chang T-H]]
-	[[Category: Hsieh, F L]]	+	[[Category: Hsieh F-L]]
-	[[Category: Ko, T P]]	+	[[Category: Ko T-P]]
-	[[Category: Wang~~, A H.~~J]]	+	[[Category: Wang AH-J]]
-	~~[[Category: Isoprene biosynthesis]]~~	+
-	~~[[Category: Isoprenyl pyrophosphate synthase]]~~	+
-	~~[[Category: Prenyltransferase]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 06:15, 16 October 2019

OCA — Wed, 16 Oct 2019 06:15:21 GMT

←Older revision		Revision as of 06:15, 16 October 2019
Line 1:		Line 1:

	==Mint heterotetrameric geranyl pyrophosphate synthase in complex with magnesium, IPP, and DMASPP (I)==		==Mint heterotetrameric geranyl pyrophosphate synthase in complex with magnesium, IPP, and DMASPP (I)==
-	<StructureSection load='3krf' size='340' side='right' caption='[[3krf]], [[Resolution\|resolution]] 2.20Å' scene=''>	+	<StructureSection load='3krf' size='340' side='right'caption='[[3krf]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3krf]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Menpi Menpi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KRF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3KRF FirstGlance]. <br>		<table><tr><td colspan='2'>[[3krf]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Menpi Menpi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KRF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3KRF FirstGlance]. <br>
Line 14:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kr/3krf_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kr/3krf_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
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	</StructureSection>		</StructureSection>
	[[Category: Dimethylallyltranstransferase]]		[[Category: Dimethylallyltranstransferase]]
		+	[[Category: Large Structures]]
	[[Category: Menpi]]		[[Category: Menpi]]
	[[Category: Chang, T H]]		[[Category: Chang, T H]]

OCA at 08:39, 1 November 2017

OCA — Wed, 01 Nov 2017 08:39:48 GMT

←Older revision		Revision as of 08:39, 1 November 2017
Line 1:		Line 1:
		+
	==Mint heterotetrameric geranyl pyrophosphate synthase in complex with magnesium, IPP, and DMASPP (I)==		==Mint heterotetrameric geranyl pyrophosphate synthase in complex with magnesium, IPP, and DMASPP (I)==
	<StructureSection load='3krf' size='340' side='right' caption='[[3krf]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='3krf' size='340' side='right' caption='[[3krf]], [[Resolution\|resolution]] 2.20Å' scene=''>
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	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GPPS Large Subunit ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=34256 MENPI]), GPPS Small subunit ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=34256 MENPI])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GPPS Large Subunit ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=34256 MENPI]), GPPS Small subunit ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=34256 MENPI])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dimethylallyltranstransferase Dimethylallyltranstransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.1 2.5.1.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dimethylallyltranstransferase Dimethylallyltranstransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.1 2.5.1.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3krf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3krf OCA], [http://pdbe.org/3krf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3krf RCSB], [http://www.ebi.ac.uk/pdbsum/3krf PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3krf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3krf OCA], [http://pdbe.org/3krf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3krf RCSB], [http://www.ebi.ac.uk/pdbsum/3krf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3krf ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 03:29, 9 February 2016

OCA — Tue, 09 Feb 2016 03:29:05 GMT

←Older revision		Revision as of 03:29, 9 February 2016
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	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GPPS Large Subunit ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=34256 MENPI]), GPPS Small subunit ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=34256 MENPI])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GPPS Large Subunit ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=34256 MENPI]), GPPS Small subunit ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=34256 MENPI])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dimethylallyltranstransferase Dimethylallyltranstransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.1 2.5.1.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dimethylallyltranstransferase Dimethylallyltranstransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.1 2.5.1.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3krf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3krf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3krf RCSB], [http://www.ebi.ac.uk/pdbsum/3krf PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3krf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3krf OCA], [http://pdbe.org/3krf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3krf RCSB], [http://www.ebi.ac.uk/pdbsum/3krf PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3krf ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3krf" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 17:30, 21 December 2014

OCA — Sun, 21 Dec 2014 17:30:49 GMT

←Older revision		Revision as of 17:30, 21 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3krf\| PDB=3krf \| SCENE= }}~~	+	==Mint heterotetrameric geranyl pyrophosphate synthase in complex with magnesium, IPP, and DMASPP (I)==
-	===Mint heterotetrameric geranyl pyrophosphate synthase in complex with magnesium, IPP, and DMASPP (I)===	+	<StructureSection load='3krf' size='340' side='right' caption='[[3krf]], [[Resolution\|resolution]] 2.20Å' scene=''>
-	~~{{ABSTRACT_PUBMED_20139160}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3krf]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Menpi Menpi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KRF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3KRF FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DST:DIMETHYLALLYL+S-THIOLODIPHOSPHATE'>DST</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=IPE:3-METHYLBUT-3-ENYL+TRIHYDROGEN+DIPHOSPHATE'>IPE</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PPV:PYROPHOSPHATE'>PPV</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3kra\|3kra]], [[3krc\|3krc]], [[3kro\|3kro]], [[3krp\|3krp]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GPPS Large Subunit ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=34256 MENPI]), GPPS Small subunit ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=34256 MENPI])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dimethylallyltranstransferase Dimethylallyltranstransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.1 2.5.1.1] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3krf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3krf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3krf RCSB], [http://www.ebi.ac.uk/pdbsum/3krf PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kr/3krf_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Terpenes (isoprenoids), derived from isoprenyl pyrophosphates, are versatile natural compounds that act as metabolism mediators, plant volatiles, and ecological communicators. Divergent evolution of homomeric prenyltransferases (PTSs) has allowed PTSs to optimize their active-site pockets to achieve catalytic fidelity and diversity. Little is known about heteromeric PTSs, particularly the mechanisms regulating formation of specific products. Here, we report the crystal structure of the (LSU . SSU)(2)-type (LSU/SSU = large/small subunit) heterotetrameric geranyl pyrophosphate synthase (GPPS) from mint (Mentha piperita). The LSU and SSU of mint GPPS are responsible for catalysis and regulation, respectively, and this SSU lacks the essential catalytic amino acid residues found in LSU and other PTSs. Whereas no activity was detected for individually expressed LSU or SSU, the intact (LSU . SSU)(2) tetramer produced not only C(10)-GPP at the beginning of the reaction but also C(20)-GGPP (geranylgeranyl pyrophosphate) at longer reaction times. The activity for synthesizing C(10)-GPP and C(20)-GGPP, but not C(15)-farnesyl pyrophosphate, reflects a conserved active-site structure of the LSU and the closely related mustard (Sinapis alba) homodimeric GGPPS. Furthermore, using a genetic complementation system, we showed that no C(20)-GGPP is produced by the mint GPPS in vivo. Presumably through protein-protein interactions, the SSU remodels the active-site cavity of LSU for synthesizing C(10)-GPP, the precursor of volatile C(10)-monoterpenes.

-	~~==About this~~ Structure==	+	Structure of a heterotetrameric geranyl pyrophosphate synthase from mint (Mentha piperita) reveals intersubunit regulation.,Chang TH, Hsieh FL, Ko TP, Teng KH, Liang PH, Wang AH Plant Cell. 2010 Feb;22(2):454-67. Epub 2010 Feb 5. PMID:20139160<ref>PMID:20139160</ref>
-	~~[[3krf]] is~~ a ~~4 chain structure with sequence~~ from ~~[http://en~~.~~wikipedia~~.~~org/wiki/Menpi Menpi]. Full crystallographic information is available from [http~~:~~//oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=3KRF OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:020139160</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Dimethylallyltranstransferase]]		[[Category: Dimethylallyltranstransferase]]
	[[Category: Menpi]]		[[Category: Menpi]]
-	[[Category: Chang, T H.]]	+	[[Category: Chang, T H]]
-	[[Category: Hsieh, F L.]]	+	[[Category: Hsieh, F L]]
-	[[Category: Ko, T P.]]	+	[[Category: Ko, T P]]
-	[[Category: Wang, A H.J.]]	+	[[Category: Wang, A H.J]]
	[[Category: Isoprene biosynthesis]]		[[Category: Isoprene biosynthesis]]
	[[Category: Isoprenyl pyrophosphate synthase]]		[[Category: Isoprenyl pyrophosphate synthase]]
	[[Category: Prenyltransferase]]		[[Category: Prenyltransferase]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 04:26, 13 February 2014

OCA — Thu, 13 Feb 2014 04:26:41 GMT

←Older revision		Revision as of 04:26, 13 February 2014
Line 4:		Line 4:

	==About this Structure==		==About this Structure==
-	[[3krf]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mentha_x_piperita Mentha x piperita~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KRF OCA].	+	[[3krf]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Menpi Menpi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KRF OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:020139160</ref><references group="xtra"/><references/>		<ref group="xtra">PMID:020139160</ref><references group="xtra"/><references/>
	[[Category: Dimethylallyltranstransferase]]		[[Category: Dimethylallyltranstransferase]]
-	[[Category: ~~Mentha x piperita~~]]	+	[[Category: Menpi]]
	[[Category: Chang, T H.]]		[[Category: Chang, T H.]]
	[[Category: Hsieh, F L.]]		[[Category: Hsieh, F L.]]

OCA: Protected "3krf" [edit=sysop:move=sysop]

OCA — Thu, 04 Apr 2013 02:37:19 GMT

Protected "3krf" [edit=sysop:move=sysop]

←Older revision

Revision as of 02:37, 4 April 2013

OCA at 02:37, 4 April 2013

OCA — Thu, 04 Apr 2013 02:37:16 GMT

←Older revision		Revision as of 02:37, 4 April 2013
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-	{{Seed}}
-	[[Image:3krf.jpg\|left\|200px]]
-
-	<!--
-	The line below this paragraph, containing "STRUCTURE_3krf", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3krf\| PDB=3krf \| SCENE= }}		{{STRUCTURE_3krf\| PDB=3krf \| SCENE= }}
-
	===Mint heterotetrameric geranyl pyrophosphate synthase in complex with magnesium, IPP, and DMASPP (I)===		===Mint heterotetrameric geranyl pyrophosphate synthase in complex with magnesium, IPP, and DMASPP (I)===
-
-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_20139160}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 20139160 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_20139160}}		{{ABSTRACT_PUBMED_20139160}}

	==About this Structure==		==About this Structure==
-	~~3KRF~~ is a 4 ~~chains~~ structure with ~~sequences~~ from [http://en.wikipedia.org/wiki/Mentha_x_piperita Mentha x piperita]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KRF OCA].	+	[[3krf]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Mentha_x_piperita Mentha x piperita]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KRF OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~20139160~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:020139160</ref><references group="xtra"/><references/>
	[[Category: Dimethylallyltranstransferase]]		[[Category: Dimethylallyltranstransferase]]
	[[Category: Mentha x piperita]]		[[Category: Mentha x piperita]]
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	[[Category: Prenyltransferase]]		[[Category: Prenyltransferase]]
	[[Category: Transferase]]		[[Category: Transferase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 25 13:38:27 2010''

OCA at 11:38, 25 February 2010

OCA — Thu, 25 Feb 2010 11:38:28 GMT

←Older revision		Revision as of 11:38, 25 February 2010
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3krf.jpg\|left\|200px]]

-	The ~~entry 3krf~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3krf", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3krf\| PDB=3krf \| SCENE= }}

-	~~Authors: Chang~~, ~~T.-H.~~, ~~Hsieh, F.-L., Ko, T.-P., Wang, A.H.-J.~~	+	===Mint heterotetrameric geranyl pyrophosphate synthase in complex with magnesium, IPP, and DMASPP (I)===

-	Description: Mint heterotetrameric geranyl pyrophosphate synthase in complex with magnesium, IPP, and DMASPP (I)

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Dec 2 09~~:39:~~11 2009~~''	+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_20139160}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 20139160 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_20139160}}
		+
		+	==About this Structure==
		+	3KRF is a 4 chains structure with sequences from [http://en.wikipedia.org/wiki/Mentha_x_piperita Mentha x piperita]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KRF OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:20139160</ref><references group="xtra"/>
		+	[[Category: Dimethylallyltranstransferase]]
		+	[[Category: Mentha x piperita]]
		+	[[Category: Chang, T H.]]
		+	[[Category: Hsieh, F L.]]
		+	[[Category: Ko, T P.]]
		+	[[Category: Wang, A H.J.]]
		+	[[Category: Isoprene biosynthesis]]
		+	[[Category: Isoprenyl pyrophosphate synthase]]
		+	[[Category: Prenyltransferase]]
		+	[[Category: Transferase]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 25 13:38:27 2010''

OCA at 07:39, 2 December 2009

OCA — Wed, 02 Dec 2009 07:39:11 GMT

←Older revision		Revision as of 07:39, 2 December 2009
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3krf is ON HOLD	+	The entry 3krf is ON HOLD until Paper Publication

-	Authors: Chang, ~~Tao~~-~~Hsin~~, Hsieh, Fu-~~Lien~~, Ko, ~~Tzu~~-~~Ping~~, Wang, ~~Andrew~~ H.-J.	+	Authors: Chang, T.-H., Hsieh, F.-L., Ko, T.-P., Wang, A.H.-J.

	Description: Mint heterotetrameric geranyl pyrophosphate synthase in complex with magnesium, IPP, and DMASPP (I)		Description: Mint heterotetrameric geranyl pyrophosphate synthase in complex with magnesium, IPP, and DMASPP (I)

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Nov 25~~ 09:15:00 2009''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 2 09:39:11 2009''