3kwg - Revision history

OCA at 06:35, 19 July 2023

OCA — Wed, 19 Jul 2023 06:35:17 GMT

←Older revision		Revision as of 06:35, 19 July 2023
Line 3:		Line 3:
	<StructureSection load='3kwg' size='340' side='right'caption='[[3kwg]], [[Resolution\|resolution]] 2.21Å' scene=''>		<StructureSection load='3kwg' size='340' side='right'caption='[[3kwg]], [[Resolution\|resolution]] 2.21Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3kwg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~I72a2 I72a2~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KWG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3KWG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3kwg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Influenza_A_virus_(A/Udorn/307/1972(H3N2)) Influenza A virus (A/Udorn/307/1972(H3N2))]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KWG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3KWG FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[3ee9\|3ee9]], [[3ee8\|3ee8]], [[3kwi\|3kwi]]</div></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.21Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">NS ([https://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=381517 I72A2])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3kwg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kwg OCA], [https://pdbe.org/3kwg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3kwg RCSB], [https://www.ebi.ac.uk/pdbsum/3kwg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3kwg ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3kwg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kwg OCA], [https://pdbe.org/3kwg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3kwg RCSB], [https://www.ebi.ac.uk/pdbsum/3kwg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3kwg ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/NS1_I72A2 NS1_I72A2]] Inhibits post-transcriptional processing of cellular pre-mRNA, by binding and inhibiting two cellular proteins that are required for the 3'-end processing of cellular pre-mRNAs: the 30 kDa cleavage and polyadenylation specificity factor (CPSF4) and the poly(A)-binding protein 2 (PABPN1). This results in the accumulation of unprocessed 3' end pre-mRNAs which can't be exported from the nucleus. Cellular protein synthesis is thereby shut off very early after virus infection. Viral protein synthesis is not affected by the inhibition of the cellular 3' end processing machinery because the poly(A) tails of viral mRNAs are produced by the viral polymerase through a stuttering mechanism.<ref>PMID:9651582</ref> <ref>PMID:9560194</ref> <ref>PMID:16571812</ref> Prevents the establishment of the cellular antiviral state by inhibiting TRIM25-mediated DDX58 ubiquitination, which normally triggers the antiviral transduction signal that leads to the activation of type I IFN genes by transcription factors like IRF3 and IRF7. Prevents human EIF2AK2/PKR activation, either by binding double-strand RNA, or by interacting directly with EIF2AK2/PKR. This function may be important at the very beginning of the infection, when NS1 is mainly present in the cytoplasm. Also binds poly(A) and U6 snRNA. Suppresses the RNA silencing-based antiviral response in Drosophila cells (By similarity).<ref>PMID:9651582</ref> <ref>PMID:9560194</ref> <ref>PMID:16571812</ref>	+	[https://www.uniprot.org/uniprot/NS1_I72A2 NS1_I72A2] Inhibits post-transcriptional processing of cellular pre-mRNA, by binding and inhibiting two cellular proteins that are required for the 3'-end processing of cellular pre-mRNAs: the 30 kDa cleavage and polyadenylation specificity factor (CPSF4) and the poly(A)-binding protein 2 (PABPN1). This results in the accumulation of unprocessed 3' end pre-mRNAs which can't be exported from the nucleus. Cellular protein synthesis is thereby shut off very early after virus infection. Viral protein synthesis is not affected by the inhibition of the cellular 3' end processing machinery because the poly(A) tails of viral mRNAs are produced by the viral polymerase through a stuttering mechanism.<ref>PMID:9651582</ref> <ref>PMID:9560194</ref> <ref>PMID:16571812</ref> Prevents the establishment of the cellular antiviral state by inhibiting TRIM25-mediated DDX58 ubiquitination, which normally triggers the antiviral transduction signal that leads to the activation of type I IFN genes by transcription factors like IRF3 and IRF7. Prevents human EIF2AK2/PKR activation, either by binding double-strand RNA, or by interacting directly with EIF2AK2/PKR. This function may be important at the very beginning of the infection, when NS1 is mainly present in the cytoplasm. Also binds poly(A) and U6 snRNA. Suppresses the RNA silencing-based antiviral response in Drosophila cells (By similarity).<ref>PMID:9651582</ref> <ref>PMID:9560194</ref> <ref>PMID:16571812</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: I72a2]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Robertus~~, J D~~]]	+	[[Category: Robertus JD]]
-	[[Category: Xia, S]]	+	[[Category: Xia S]]
-	~~[[Category: Effector domain]]~~	+
-	~~[[Category: Host cytoplasm]]~~	+
-	~~[[Category: Host nucleus]]~~	+
-	~~[[Category: Host-virus interaction]]~~	+
-	~~[[Category: Influenza]]~~	+
-	~~[[Category: Interferon antiviral system evasion]]~~	+
-	~~[[Category: Ns1]]~~	+
-	~~[[Category: Rna-binding]]~~	+
-	~~[[Category: Suppressor of rna silencing]]~~	+
-	~~[[Category: Viral protein~~]]	+

OCA at 12:29, 13 October 2021

OCA — Wed, 13 Oct 2021 12:29:37 GMT

←Older revision		Revision as of 12:29, 13 October 2021
Line 1:		Line 1:

	==X-ray structure of NS1 effector domain W187A mutant==		==X-ray structure of NS1 effector domain W187A mutant==
-	<StructureSection load='3kwg' size='340' side='right' caption='[[3kwg]], [[Resolution\|resolution]] 2.21Å' scene=''>	+	<StructureSection load='3kwg' size='340' side='right'caption='[[3kwg]], [[Resolution\|resolution]] 2.21Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3kwg]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/I72a2 I72a2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KWG OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3KWG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3kwg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/I72a2 I72a2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KWG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3KWG FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ee9\|3ee9]], [[3ee8\|3ee8]], [[3kwi\|3kwi]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3ee9\|3ee9]], [[3ee8\|3ee8]], [[3kwi\|3kwi]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NS ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=381517 I72A2])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NS ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=381517 I72A2])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3kwg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kwg OCA], [~~http~~://pdbe.org/3kwg PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3kwg RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3kwg PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3kwg ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3kwg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kwg OCA], [https://pdbe.org/3kwg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3kwg RCSB], [https://www.ebi.ac.uk/pdbsum/3kwg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3kwg ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/NS1_I72A2 NS1_I72A2]] Inhibits post-transcriptional processing of cellular pre-mRNA, by binding and inhibiting two cellular proteins that are required for the 3'-end processing of cellular pre-mRNAs: the 30 kDa cleavage and polyadenylation specificity factor (CPSF4) and the poly(A)-binding protein 2 (PABPN1). This results in the accumulation of unprocessed 3' end pre-mRNAs which can't be exported from the nucleus. Cellular protein synthesis is thereby shut off very early after virus infection. Viral protein synthesis is not affected by the inhibition of the cellular 3' end processing machinery because the poly(A) tails of viral mRNAs are produced by the viral polymerase through a stuttering mechanism.<ref>PMID:9651582</ref> <ref>PMID:9560194</ref> <ref>PMID:16571812</ref> Prevents the establishment of the cellular antiviral state by inhibiting TRIM25-mediated DDX58 ubiquitination, which normally triggers the antiviral transduction signal that leads to the activation of type I IFN genes by transcription factors like IRF3 and IRF7. Prevents human EIF2AK2/PKR activation, either by binding double-strand RNA, or by interacting directly with EIF2AK2/PKR. This function may be important at the very beginning of the infection, when NS1 is mainly present in the cytoplasm. Also binds poly(A) and U6 snRNA. Suppresses the RNA silencing-based antiviral response in Drosophila cells (By similarity).<ref>PMID:9651582</ref> <ref>PMID:9560194</ref> <ref>PMID:16571812</ref>	+	[[https://www.uniprot.org/uniprot/NS1_I72A2 NS1_I72A2]] Inhibits post-transcriptional processing of cellular pre-mRNA, by binding and inhibiting two cellular proteins that are required for the 3'-end processing of cellular pre-mRNAs: the 30 kDa cleavage and polyadenylation specificity factor (CPSF4) and the poly(A)-binding protein 2 (PABPN1). This results in the accumulation of unprocessed 3' end pre-mRNAs which can't be exported from the nucleus. Cellular protein synthesis is thereby shut off very early after virus infection. Viral protein synthesis is not affected by the inhibition of the cellular 3' end processing machinery because the poly(A) tails of viral mRNAs are produced by the viral polymerase through a stuttering mechanism.<ref>PMID:9651582</ref> <ref>PMID:9560194</ref> <ref>PMID:16571812</ref> Prevents the establishment of the cellular antiviral state by inhibiting TRIM25-mediated DDX58 ubiquitination, which normally triggers the antiviral transduction signal that leads to the activation of type I IFN genes by transcription factors like IRF3 and IRF7. Prevents human EIF2AK2/PKR activation, either by binding double-strand RNA, or by interacting directly with EIF2AK2/PKR. This function may be important at the very beginning of the infection, when NS1 is mainly present in the cytoplasm. Also binds poly(A) and U6 snRNA. Suppresses the RNA silencing-based antiviral response in Drosophila cells (By similarity).<ref>PMID:9651582</ref> <ref>PMID:9560194</ref> <ref>PMID:16571812</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: I72a2]]		[[Category: I72a2]]
		+	[[Category: Large Structures]]
	[[Category: Robertus, J D]]		[[Category: Robertus, J D]]
	[[Category: Xia, S]]		[[Category: Xia, S]]

OCA at 09:12, 5 August 2016

OCA — Fri, 05 Aug 2016 09:12:22 GMT

←Older revision		Revision as of 09:12, 5 August 2016
Line 1:		Line 1:
		+
	==X-ray structure of NS1 effector domain W187A mutant==		==X-ray structure of NS1 effector domain W187A mutant==
	<StructureSection load='3kwg' size='340' side='right' caption='[[3kwg]], [[Resolution\|resolution]] 2.21Å' scene=''>		<StructureSection load='3kwg' size='340' side='right' caption='[[3kwg]], [[Resolution\|resolution]] 2.21Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3kwg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Influenza_a_virus Influenza a virus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KWG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3KWG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3kwg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/I72a2 I72a2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KWG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3KWG FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ee9\|3ee9]], [[3ee8\|3ee8]], [[3kwi\|3kwi]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ee9\|3ee9]], [[3ee8\|3ee8]], [[3kwi\|3kwi]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~11320 Influenza A virus~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=381517 I72A2])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3kwg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kwg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3kwg RCSB], [http://www.ebi.ac.uk/pdbsum/3kwg PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3kwg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kwg OCA], [http://pdbe.org/3kwg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3kwg RCSB], [http://www.ebi.ac.uk/pdbsum/3kwg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3kwg ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3kwg" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Influenza a virus~~]]	+	[[Category: I72a2]]
	[[Category: Robertus, J D]]		[[Category: Robertus, J D]]
	[[Category: Xia, S]]		[[Category: Xia, S]]

OCA at 01:04, 25 December 2014

OCA — Thu, 25 Dec 2014 01:04:43 GMT

←Older revision		Revision as of 01:04, 25 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3kwg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kwg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3kwg RCSB], [http://www.ebi.ac.uk/pdbsum/3kwg PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3kwg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kwg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3kwg RCSB], [http://www.ebi.ac.uk/pdbsum/3kwg PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/NS1_I72A2 NS1_I72A2]] Inhibits post-transcriptional processing of cellular pre-mRNA, by binding and inhibiting two cellular proteins that are required for the 3'-end processing of cellular pre-mRNAs: the 30 kDa cleavage and polyadenylation specificity factor (CPSF4) and the poly(A)-binding protein 2 (PABPN1). This results in the accumulation of unprocessed 3' end pre-mRNAs which can't be exported from the nucleus. Cellular protein synthesis is thereby shut off very early after virus infection. Viral protein synthesis is not affected by the inhibition of the cellular 3' end processing machinery because the poly(A) tails of viral mRNAs are produced by the viral polymerase through a stuttering mechanism.<ref>PMID:9651582</ref> <ref>PMID:9560194</ref> <ref>PMID:16571812</ref> Prevents the establishment of the cellular antiviral state by inhibiting TRIM25-mediated DDX58 ubiquitination, which normally triggers the antiviral transduction signal that leads to the activation of type I IFN genes by transcription factors like IRF3 and IRF7. Prevents human EIF2AK2/PKR activation, either by binding double-strand RNA, or by interacting directly with EIF2AK2/PKR. This function may be important at the very beginning of the infection, when NS1 is mainly present in the cytoplasm. Also binds poly(A) and U6 snRNA. Suppresses the RNA silencing-based antiviral response in Drosophila cells (By similarity).<ref>PMID:9651582</ref> <ref>PMID:9560194</ref> <ref>PMID:16571812</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 15:45, 18 December 2014

OCA — Thu, 18 Dec 2014 15:45:48 GMT

←Older revision		Revision as of 15:45, 18 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3kwg\| PDB=3kwg \| SCENE= }}~~	+	==X-ray structure of NS1 effector domain W187A mutant==
-	===X-ray structure of NS1 effector domain W187A mutant===	+	<StructureSection load='3kwg' size='340' side='right' caption='[[3kwg]], [[Resolution\|resolution]] 2.21Å' scene=''>
-	~~{{ABSTRACT_PUBMED_19995550}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3kwg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Influenza_a_virus Influenza a virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KWG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3KWG FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ee9\|3ee9]], [[3ee8\|3ee8]], [[3kwi\|3kwi]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11320 Influenza A virus])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3kwg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kwg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3kwg RCSB], [http://www.ebi.ac.uk/pdbsum/3kwg PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The influenza A virus nonstructural protein NS1 is a multifunctional dimeric protein that acts as a potent inhibitor of the host cellular antiviral state. The C-terminal effector domain of NS1 binds host proteins, including CPSF30, and is a target for the development of new antiviral drugs. Here we present crystallographic structures of two mutant effector domains, W187Y and W187A, of influenza A/Udorn/72 virus. Unlike wild-type, the mutants behave exclusively as monomers in solution based on gel filtration data and light scattering. The W187Y mutant is able to bind CPSF30 with a binding affinity close to the wild-type protein; that is, it retains a receptor site for aromatic ligands nearly identical to the wild-type. Therefore, this monomeric mutant protein could serve as a drug target for a high throughput inhibitor screening assays, since its binding pocket is unoccupied in solution and potentially more accessible to small molecule ligands.

-	~~==Function==~~	+	X-ray structures of NS1 effector domain mutants.,Xia S, Robertus JD Arch Biochem Biophys. 2010 Feb 15;494(2):198-204. Epub 2009 Dec 6. PMID:19995550<ref>PMID:19995550</ref>
-	~~[[http://www.uniprot.org/uniprot/NS1_I72A2 NS1_I72A2]] Inhibits post~~-~~transcriptional processing~~ of ~~cellular pre-mRNA~~, ~~by binding and inhibiting two cellular proteins that are required for the 3'-end processing of cellular pre-mRNAs: the 30 kDa cleavage and polyadenylation specificity factor~~ (~~CPSF4) and the poly(A)-binding protein~~ 2 ~~(PABPN1~~). This results in the accumulation of unprocessed 3' end pre-mRNAs which can't be exported from the nucleus. Cellular protein synthesis is thereby shut off very early after virus infection. Viral protein synthesis is not affected by the inhibition of the cellular 3' end processing machinery because the poly(A) tails of viral mRNAs are produced by the viral polymerase through a stuttering mechanism.<ref>PMID:~~9651582</ref> <ref>PMID:9560194</ref> <ref>PMID:16571812</ref> Prevents the establishment of the cellular antiviral state by inhibiting TRIM25~~-~~mediated DDX58 ubiquitination, which normally triggers the antiviral transduction signal that leads to the activation of type I IFN genes by transcription factors like IRF3 and IRF7~~. ~~Prevents human EIF2AK2/PKR activation, either by binding double-strand RNA, or by interacting directly with EIF2AK2/PKR~~. This function may be important at the very beginning of the infection, when NS1 is mainly present in the cytoplasm. Also binds poly(A) and U6 snRNA. Suppresses the RNA silencing-based antiviral response in Drosophila cells (By similarity).<ref>PMID:~~9651582</ref> <ref>PMID:9560194</ref>~~ <ref>PMID:~~16571812~~</ref>	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[3kwg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki~~/~~Influenza_a_virus Influenza~~ a ~~virus]~~. ~~Full crystallographic information is available from [http://oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=3KWG OCA].~~	+	</div>
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:019995550</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Influenza a virus]]		[[Category: Influenza a virus]]
-	[[Category: Robertus, J D.]]	+	[[Category: Robertus, J D]]
-	[[Category: Xia, S.]]	+	[[Category: Xia, S]]
	[[Category: Effector domain]]		[[Category: Effector domain]]
	[[Category: Host cytoplasm]]		[[Category: Host cytoplasm]]

OCA: Protected "3kwg" [edit=sysop:move=sysop]

OCA — Thu, 04 Apr 2013 19:56:56 GMT

Protected "3kwg" [edit=sysop:move=sysop]

←Older revision

Revision as of 19:56, 4 April 2013

OCA at 19:56, 4 April 2013

OCA — Thu, 04 Apr 2013 19:56:52 GMT

←Older revision		Revision as of 19:56, 4 April 2013
Line 1:		Line 1:
-	{{Seed}}
-	[[Image:3kwg.png\|left\|200px]]
-
-	<!--
-	The line below this paragraph, containing "STRUCTURE_3kwg", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3kwg\| PDB=3kwg \| SCENE= }}		{{STRUCTURE_3kwg\| PDB=3kwg \| SCENE= }}
-
	===X-ray structure of NS1 effector domain W187A mutant===		===X-ray structure of NS1 effector domain W187A mutant===
		+	{{ABSTRACT_PUBMED_19995550}}

-		+	==Function==
-	<!--	+	[[http://www.uniprot.org/uniprot/NS1_I72A2 NS1_I72A2]] Inhibits post-transcriptional processing of cellular pre-mRNA, by binding and inhibiting two cellular proteins that are required for the 3'-end processing of cellular pre-mRNAs: the 30 kDa cleavage and polyadenylation specificity factor (CPSF4) and the poly(A)-binding protein 2 (PABPN1). This results in the accumulation of unprocessed 3' end pre-mRNAs which can't be exported from the nucleus. Cellular protein synthesis is thereby shut off very early after virus infection. Viral protein synthesis is not affected by the inhibition of the cellular 3' end processing machinery because the poly(A) tails of viral mRNAs are produced by the viral polymerase through a stuttering mechanism.<ref>PMID:9651582</ref> <ref>PMID:9560194</ref> <ref>PMID:16571812</ref> Prevents the establishment of the cellular antiviral state by inhibiting TRIM25-mediated DDX58 ubiquitination, which normally triggers the antiviral transduction signal that leads to the activation of type I IFN genes by transcription factors like IRF3 and IRF7. Prevents human EIF2AK2/PKR activation, either by binding double-strand RNA, or by interacting directly with EIF2AK2/PKR. This function may be important at the very beginning of the infection, when NS1 is mainly present in the cytoplasm. Also binds poly(A) and U6 snRNA. Suppresses the RNA silencing-based antiviral response in Drosophila cells (By similarity).<ref>PMID:9651582</ref> <ref>PMID:9560194</ref> <ref>PMID:16571812</ref>
-	~~The line below this paragraph~~, ~~{{ABSTRACT_PUBMED_19995550}}, adds~~ the ~~Publication Abstract to~~ the ~~page~~	+
-	(~~as it appears on PubMed at http~~://~~www~~.~~pubmed~~.~~gov)~~, ~~where 19995550~~ is the ~~PubMed ID number~~.	+
-	-->	+
-	~~{{ABSTRACT_PUBMED_19995550}}~~	+

	==About this Structure==		==About this Structure==
-	~~3KWG~~ is a 2 ~~chains~~ structure with ~~sequences~~ from [http://en.wikipedia.org/wiki/Influenza_a_virus Influenza a virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KWG OCA].	+	[[3kwg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Influenza_a_virus Influenza a virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KWG OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~19995550~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019995550</ref><references group="xtra"/><references/>
	[[Category: Influenza a virus]]		[[Category: Influenza a virus]]
	[[Category: Robertus, J D.]]		[[Category: Robertus, J D.]]
Line 37:		Line 24:
	[[Category: Suppressor of rna silencing]]		[[Category: Suppressor of rna silencing]]
	[[Category: Viral protein]]		[[Category: Viral protein]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 24 08:21:49 2010''

OCA at 06:21, 24 March 2010

OCA — Wed, 24 Mar 2010 06:21:50 GMT

←Older revision		Revision as of 06:21, 24 March 2010
Line 1:		Line 1:
	{{Seed}}		{{Seed}}
-	[[Image:3kwg.~~jpg~~\|left\|200px]]	+	[[Image:3kwg.png\|left\|200px]]

	<!--		<!--
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	[[Category: Rna-binding]]		[[Category: Rna-binding]]
	[[Category: Suppressor of rna silencing]]		[[Category: Suppressor of rna silencing]]
		+	[[Category: Viral protein]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Feb 3 09~~:29:54 2010''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 24 08:21:49 2010''

OCA at 07:29, 3 February 2010

OCA — Wed, 03 Feb 2010 07:29:56 GMT

←Older revision		Revision as of 07:29, 3 February 2010
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3kwg.jpg\|left\|200px]]

-	The ~~entry 3kwg~~ is ~~ON HOLD~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3kwg", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3kwg\| PDB=3kwg \| SCENE= }}

-	~~Authors: Xia, S., Robertus, J.D.~~	+	===X-ray structure of NS1 effector domain W187A mutant===

-	Description: X-ray structure of NS1 effector domain W187A mutant

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Dec~~ ~~9 14~~:45:~~27 2009~~''	+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_19995550}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 19995550 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_19995550}}
		+
		+	==About this Structure==
		+	3KWG is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Influenza_a_virus Influenza a virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KWG OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:19995550</ref><references group="xtra"/>
		+	[[Category: Influenza a virus]]
		+	[[Category: Robertus, J D.]]
		+	[[Category: Xia, S.]]
		+	[[Category: Effector domain]]
		+	[[Category: Host cytoplasm]]
		+	[[Category: Host nucleus]]
		+	[[Category: Host-virus interaction]]
		+	[[Category: Influenza]]
		+	[[Category: Interferon antiviral system evasion]]
		+	[[Category: Ns1]]
		+	[[Category: Rna-binding]]
		+	[[Category: Suppressor of rna silencing]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 3 09:29:54 2010''

OCA: New page: '''Unreleased structure''' The entry 3kwg is ON HOLD Authors: Xia, S., Robertus, J.D. Description: X-ray structure of NS1 effector domain W187A mutant ''Page seeded by [http://oca.wei...

OCA — Wed, 09 Dec 2009 12:45:28 GMT

New page: '''Unreleased structure''' The entry 3kwg is ON HOLD Authors: Xia, S., Robertus, J.D. Description: X-ray structure of NS1 effector domain W187A mutant ''Page seeded by [http://oca.wei...

New page

'''Unreleased structure'''

The entry 3kwg is ON HOLD

Authors: Xia, S., Robertus, J.D.

Description: X-ray structure of NS1 effector domain W187A mutant

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 9 14:45:27 2009''