3kz1 - Revision history

OCA at 08:27, 6 September 2023

OCA — Wed, 06 Sep 2023 08:27:27 GMT

←Older revision		Revision as of 08:27, 6 September 2023
Line 1:		Line 1:

	==Crystal Structure of the Complex of PDZ-RhoGEF DH/PH domains with GTP-gamma-S Activated RhoA==		==Crystal Structure of the Complex of PDZ-RhoGEF DH/PH domains with GTP-gamma-S Activated RhoA==
-	<StructureSection load='3kz1' size='340' side='right' caption='[[3kz1]], [[Resolution\|resolution]] 2.70Å' scene=''>	+	<StructureSection load='3kz1' size='340' side='right'caption='[[3kz1]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3kz1]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KZ1 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3KZ1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3kz1]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KZ1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3KZ1 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=GSP:5~~-~~GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE'>GSP</scene>~~, ~~<scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.7Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~>[[1xcg\|1xcg]], [[1x86\|1x86]], [[1txd\|1txd]], [[1a2b\|1a2b]]</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GSP:5-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE'>GSP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~"><~~b>[[Gene\|Gene~~:~~]]</b~~></td><~~td class~~=~~"sblockDat">ARHGEF11, KIAA0380 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode=5&id=9606 HUMAN]), RHOA, ARH12, ARHA, RHO12 ([http~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3kz1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kz1 OCA], [https://pdbe.org/3kz1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3kz1 RCSB], [https://www.ebi.ac.uk/pdbsum/3kz1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3kz1 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3kz1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kz1 OCA], [~~http~~://pdbe.org/3kz1 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3kz1 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3kz1 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3kz1 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/ARHGB_HUMAN ARHGB_HUMAN]] May play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13). Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13. [[http://www.uniprot.org/uniprot/RHOA_HUMAN RHOA_HUMAN]] Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. Stimulates PKN2 kinase activity. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization.<ref>PMID:8910519</ref> <ref>PMID:9121475</ref> <ref>PMID:12900402</ref> <ref>PMID:16103226</ref> <ref>PMID:16236794</ref> <ref>PMID:19934221</ref> <ref>PMID:20937854</ref> <ref>PMID:20974804</ref>	+	[https://www.uniprot.org/uniprot/RHOA_HUMAN RHOA_HUMAN] Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. Stimulates PKN2 kinase activity. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization.<ref>PMID:8910519</ref> <ref>PMID:9121475</ref> <ref>PMID:12900402</ref> <ref>PMID:16103226</ref> <ref>PMID:16236794</ref> <ref>PMID:19934221</ref> <ref>PMID:20937854</ref> <ref>PMID:20974804</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	==See Also==		==See Also==
-	*[[Rho GTPase\|Rho GTPase]]	+	*[[Rho GTPase 3D structures\|Rho GTPase 3D structures]]
	*[[Rho guanine nucleotide exchange factor\|Rho guanine nucleotide exchange factor]]		*[[Rho guanine nucleotide exchange factor\|Rho guanine nucleotide exchange factor]]
		+	*[[Rho guanine nucleotide exchange factor 3D structures\|Rho guanine nucleotide exchange factor 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
-	[[Category: ~~Chen, Z~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Sprang, S R~~]]	+	[[Category: Chen Z]]
-	[[Category: ~~Sternweis, P C~~]]	+	[[Category: Sprang SR]]
-	[[Category: ~~Cytoskeleton]]~~	+	[[Category: Sternweis PC]]
-	~~[[Category: Dh]]~~	+
-	~~[[Category: Gtp-binding]]~~	+
-	~~[[Category: Gtpase activation]]~~	+
-	~~[[Category: Guanine-nucleotide releasing factor]]~~	+
-	~~[[Category: Magnesium]]~~	+
-	~~[[Category: Membrane]]~~	+
-	~~[[Category: Nucleotide-binding]]~~	+
-	~~[[Category: Ph]]~~	+
-	~~[[Category: Prenylation]]~~	+
-	~~[[Category: Proto-oncogene]]~~	+
-	~~[[Category: Regulation of rhoa gtpase]]~~	+
-	~~[[Category: Rho]]~~	+
-	~~[[Category: Rhogef]]~~	+
-	~~[[Category: Signaling protein~~]]	+

OCA at 06:49, 2 January 2019

OCA — Wed, 02 Jan 2019 06:49:42 GMT

←Older revision		Revision as of 06:49, 2 January 2019
Line 15:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kz/3kz1_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kz/3kz1_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 32:		Line 32:

	==See Also==		==See Also==
		+	*[[Rho GTPase\|Rho GTPase]]
	*[[Rho guanine nucleotide exchange factor\|Rho guanine nucleotide exchange factor]]		*[[Rho guanine nucleotide exchange factor\|Rho guanine nucleotide exchange factor]]
	== References ==		== References ==

OCA at 14:43, 5 August 2016

OCA — Fri, 05 Aug 2016 14:43:11 GMT

←Older revision		Revision as of 14:43, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal Structure of the Complex of PDZ-RhoGEF DH/PH domains with GTP-gamma-S Activated RhoA==		==Crystal Structure of the Complex of PDZ-RhoGEF DH/PH domains with GTP-gamma-S Activated RhoA==
	<StructureSection load='3kz1' size='340' side='right' caption='[[3kz1]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='3kz1' size='340' side='right' caption='[[3kz1]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3kz1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KZ1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3KZ1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3kz1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KZ1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3KZ1 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GSP:5-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE'>GSP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GSP:5-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE'>GSP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1xcg\|1xcg]], [[1x86\|1x86]], [[1txd\|1txd]], [[1a2b\|1a2b]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1xcg\|1xcg]], [[1x86\|1x86]], [[1txd\|1txd]], [[1a2b\|1a2b]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ARHGEF11, KIAA0380 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~]), RHOA, ARH12, ARHA, RHO12 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ARHGEF11, KIAA0380 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), RHOA, ARH12, ARHA, RHO12 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3kz1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kz1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3kz1 RCSB], [http://www.ebi.ac.uk/pdbsum/3kz1 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3kz1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kz1 OCA], [http://pdbe.org/3kz1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3kz1 RCSB], [http://www.ebi.ac.uk/pdbsum/3kz1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3kz1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3kz1 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3kz1" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Rho guanine nucleotide exchange factor\|Rho guanine nucleotide exchange factor]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Chen, Z]]		[[Category: Chen, Z]]
	[[Category: Sprang, S R]]		[[Category: Sprang, S R]]

OCA at 19:39, 25 December 2014

OCA — Thu, 25 Dec 2014 19:39:53 GMT

←Older revision		Revision as of 19:39, 25 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3kz1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kz1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3kz1 RCSB], [http://www.ebi.ac.uk/pdbsum/3kz1 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3kz1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kz1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3kz1 RCSB], [http://www.ebi.ac.uk/pdbsum/3kz1 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ARHGB_HUMAN ARHGB_HUMAN]] May play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13). Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13. [[http://www.uniprot.org/uniprot/RHOA_HUMAN RHOA_HUMAN]] Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. Stimulates PKN2 kinase activity. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization.<ref>PMID:8910519</ref> <ref>PMID:9121475</ref> <ref>PMID:12900402</ref> <ref>PMID:16103226</ref> <ref>PMID:16236794</ref> <ref>PMID:19934221</ref> <ref>PMID:20937854</ref> <ref>PMID:20974804</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 16:02, 18 December 2014

OCA — Thu, 18 Dec 2014 16:02:40 GMT

←Older revision		Revision as of 16:02, 18 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3kz1\| PDB=3kz1 \| SCENE= }}~~	+	==Crystal Structure of the Complex of PDZ-RhoGEF DH/PH domains with GTP-gamma-S Activated RhoA==
-	===Crystal Structure of the Complex of PDZ-RhoGEF DH/PH domains with GTP-gamma-S Activated RhoA===	+	<StructureSection load='3kz1' size='340' side='right' caption='[[3kz1]], [[Resolution\|resolution]] 2.70Å' scene=''>
-	~~{{ABSTRACT_PUBMED_20430886}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3kz1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KZ1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3KZ1 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GSP:5-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE'>GSP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1xcg\|1xcg]], [[1x86\|1x86]], [[1txd\|1txd]], [[1a2b\|1a2b]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ARHGEF11, KIAA0380 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), RHOA, ARH12, ARHA, RHO12 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3kz1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kz1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3kz1 RCSB], [http://www.ebi.ac.uk/pdbsum/3kz1 PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kz/3kz1_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Guanine nucleotide exchange factors (GEFs) catalyze exchange of GDP for GTP by stabilizing the nucleotide-free state of the small GTPases through their Dbl homology/pleckstrin homology (DH.PH) domains. Unconventionally, PDZ-RhoGEF (PRG), a member of the RGS-RhoGEFs, binds tightly to both nucleotide-free and activated RhoA (RhoA.GTP). We have characterized the interaction between PRG and activated RhoA and determined the structure of the PRG-DH.PH-RhoA.GTPgammaS (guanosine 5'-O-[gamma-thio]triphosphate) complex. The interface bears striking similarity to a GTPase-effector interface and involves the switch regions in RhoA and a hydrophobic patch in PRG-PH that is conserved among all Lbc RhoGEFs. The two surfaces that bind activated and nucleotide-free RhoA on PRG-DH.PH do not overlap, and a ternary complex of PRG-DH.PH bound to both forms of RhoA can be isolated by size-exclusion chromatography. This novel interaction between activated RhoA and PH could play a key role in regulation of RhoGEF activity in vivo.

-	~~==Function==~~	+	Activated RhoA binds to the pleckstrin homology (PH) domain of PDZ-RhoGEF, a potential site for autoregulation.,Chen Z, Medina F, Liu MY, Thomas C, Sprang SR, Sternweis PC J Biol Chem. 2010 Jul 2;285(27):21070-81. Epub 2010 Apr 29. PMID:20430886<ref>PMID:20430886</ref>
-	~~[[http://www.uniprot.org/uniprot/ARHGB_HUMAN ARHGB_HUMAN]] May play a role in~~ the ~~regulation of RhoA GTPase by guanine nucleotide-binding alpha-12~~ (~~GNA12~~) ~~and alpha~~-13 (GNA13). Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13. [[http://www.uniprot.org/uniprot/RHOA_HUMAN RHOA_HUMAN]] Regulates a ~~signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required~~ for ~~the myosin contractile ring formation during cell cycle cytokinesis~~. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, ~~vector of the plague~~, ~~and Yersinia pseudotuberculosis~~, ~~which causes gastrointestinal disorders. Stimulates PKN2 kinase activity. May be an activator of PLCE1. Activated by ARHGEF2~~, ~~which promotes the exchange of GDP for GTP~~. ~~Essential for the SPATA13~~-~~mediated regulation of cell migration and adhesion assembly and disassembly~~. ~~The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex~~. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization.<ref>PMID:~~8910519</ref> <ref>PMID:9121475</ref> <ref>PMID:12900402</ref> <ref>PMID:16103226</ref> <ref>PMID:16236794</ref> <ref>PMID:19934221</ref> <ref>PMID:20937854</ref>~~ <ref>PMID:~~20974804~~</ref>	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[3kz1]] is~~ a ~~4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3KZ1 OCA].~~	+	</div>
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:020430886</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Chen, Z.]]	+	[[Category: Chen, Z]]
-	[[Category: Sprang, S R.]]	+	[[Category: Sprang, S R]]
-	[[Category: Sternweis, P C.]]	+	[[Category: Sternweis, P C]]
	[[Category: Cytoskeleton]]		[[Category: Cytoskeleton]]
	[[Category: Dh]]		[[Category: Dh]]

OCA: Protected "3kz1" [edit=sysop:move=sysop]

OCA — Thu, 04 Apr 2013 20:33:05 GMT

Protected "3kz1" [edit=sysop:move=sysop]

←Older revision

Revision as of 20:33, 4 April 2013

OCA at 20:32, 4 April 2013

OCA — Thu, 04 Apr 2013 20:32:57 GMT

←Older revision		Revision as of 20:32, 4 April 2013
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-	{{Seed}}
-	[[Image:3kz1.png\|left\|200px]]
-
-	<!--
-	The line below this paragraph, containing "STRUCTURE_3kz1", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3kz1\| PDB=3kz1 \| SCENE= }}		{{STRUCTURE_3kz1\| PDB=3kz1 \| SCENE= }}
-
	===Crystal Structure of the Complex of PDZ-RhoGEF DH/PH domains with GTP-gamma-S Activated RhoA===		===Crystal Structure of the Complex of PDZ-RhoGEF DH/PH domains with GTP-gamma-S Activated RhoA===
		+	{{ABSTRACT_PUBMED_20430886}}

-		+	==Function==
-	<!--	+	[[http://www.uniprot.org/uniprot/ARHGB_HUMAN ARHGB_HUMAN]] May play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13). Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13. [[http://www.uniprot.org/uniprot/RHOA_HUMAN RHOA_HUMAN]] Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. Stimulates PKN2 kinase activity. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization.<ref>PMID:8910519</ref> <ref>PMID:9121475</ref> <ref>PMID:12900402</ref> <ref>PMID:16103226</ref> <ref>PMID:16236794</ref> <ref>PMID:19934221</ref> <ref>PMID:20937854</ref> <ref>PMID:20974804</ref>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_20430886}}, adds the Publication Abstract to the page~~	+
-	(as ~~it appears on PubMed at~~ http://www.~~pubmed~~.~~gov), where 20430886~~ is the ~~PubMed ID number~~.	+
-	-->	+
-	~~{{ABSTRACT_PUBMED_20430886}}~~	+

	==About this Structure==		==About this Structure==
-	~~3KZ1~~ is a 4 ~~chains~~ structure with ~~sequences~~ from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KZ1 OCA].	+	[[3kz1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KZ1 OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~20430886~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:020430886</ref><references group="xtra"/><references/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Chen, Z.]]		[[Category: Chen, Z.]]
Line 43:		Line 30:
	[[Category: Rhogef]]		[[Category: Rhogef]]
	[[Category: Signaling protein]]		[[Category: Signaling protein]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 28 12:08:04 2010''

OCA at 08:01, 28 July 2010

OCA — Wed, 28 Jul 2010 08:01:22 GMT

←Older revision		Revision as of 08:01, 28 July 2010
Line 1:		Line 1:
	{{Seed}}		{{Seed}}
-	[[Image:3kz1.~~jpg~~\|left\|200px]]	+	[[Image:3kz1.png\|left\|200px]]

	<!--		<!--
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	===Crystal Structure of the Complex of PDZ-RhoGEF DH/PH domains with GTP-gamma-S Activated RhoA===		===Crystal Structure of the Complex of PDZ-RhoGEF DH/PH domains with GTP-gamma-S Activated RhoA===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_20430886}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 20430886 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_20430886}}

	==About this Structure==		==About this Structure==
	3KZ1 is a 4 chains structure with sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KZ1 OCA].		3KZ1 is a 4 chains structure with sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KZ1 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:20430886</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Chen, Z.]]		[[Category: Chen, Z.]]
	[[Category: Sprang, S R.]]		[[Category: Sprang, S R.]]
	[[Category: Sternweis, P C.]]		[[Category: Sternweis, P C.]]
-	[[Category: Coiled coil]]
	[[Category: Cytoskeleton]]		[[Category: Cytoskeleton]]
	[[Category: Dh]]		[[Category: Dh]]
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	[[Category: Signaling protein]]		[[Category: Signaling protein]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Apr~~ 28 11:07:05 2010''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 28 12:08:04 2010''

OCA at 08:07, 28 April 2010

OCA — Wed, 28 Apr 2010 08:07:06 GMT

←Older revision		Revision as of 08:07, 28 April 2010
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-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3kz1.jpg\|left\|200px]]

-	The ~~entry 3kz1~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3kz1", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3kz1\| PDB=3kz1 \| SCENE= }}

-	~~Authors: Chen, Z., Sternweis, P.C., Sprang,~~ S~~.R.~~	+	===Crystal Structure of the Complex of PDZ-RhoGEF DH/PH domains with GTP-gamma-S Activated RhoA===

-	Description: Crystal Structure of the Complex of PDZ-RhoGEF DH/PH domains with GTP-gamma-S Activated RhoA

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Mar 31 13~~:20:26 2010''	+	==About this Structure==
		+	3KZ1 is a 4 chains structure with sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KZ1 OCA].
		+	[[Category: Homo sapiens]]
		+	[[Category: Chen, Z.]]
		+	[[Category: Sprang, S R.]]
		+	[[Category: Sternweis, P C.]]
		+	[[Category: Coiled coil]]
		+	[[Category: Cytoskeleton]]
		+	[[Category: Dh]]
		+	[[Category: Gtp-binding]]
		+	[[Category: Gtpase activation]]
		+	[[Category: Guanine-nucleotide releasing factor]]
		+	[[Category: Magnesium]]
		+	[[Category: Membrane]]
		+	[[Category: Nucleotide-binding]]
		+	[[Category: Ph]]
		+	[[Category: Prenylation]]
		+	[[Category: Proto-oncogene]]
		+	[[Category: Regulation of rhoa gtpase]]
		+	[[Category: Rho]]
		+	[[Category: Rhogef]]
		+	[[Category: Signaling protein]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 28 11:07:05 2010''

OCA at 10:20, 31 March 2010

OCA — Wed, 31 Mar 2010 10:20:26 GMT

←Older revision		Revision as of 10:20, 31 March 2010
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3kz1 is ON HOLD	+	The entry 3kz1 is ON HOLD until Paper Publication

	Authors: Chen, Z., Sternweis, P.C., Sprang, S.R.		Authors: Chen, Z., Sternweis, P.C., Sprang, S.R.
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	Description: Crystal Structure of the Complex of PDZ-RhoGEF DH/PH domains with GTP-gamma-S Activated RhoA		Description: Crystal Structure of the Complex of PDZ-RhoGEF DH/PH domains with GTP-gamma-S Activated RhoA

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Dec 30~~ 13:47:~~52 2009~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 31 13:20:26 2010''