3l27 - Revision history

OCA at 06:35, 19 July 2023

OCA — Wed, 19 Jul 2023 06:35:23 GMT

←Older revision		Revision as of 06:35, 19 July 2023
Line 3:		Line 3:
	<StructureSection load='3l27' size='340' side='right'caption='[[3l27]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='3l27' size='340' side='right'caption='[[3l27]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3l27]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Ebov~~-~~may Ebov~~-~~may~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L27 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3L27 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3l27]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Ebola_virus_-_Mayinga,_Zaire,_1976 Ebola virus - Mayinga, Zaire, 1976]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L27 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3L27 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.95Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3fke\|3fke]], [[3l25\|3l25]], [[3l26\|3l26]], [[3l28\|3l28]], [[3l29\|3l29]], [[3l2a\|3l2a]]</div></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">VP35 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=128952 EBOV-May])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3l27 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3l27 OCA], [https://pdbe.org/3l27 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3l27 RCSB], [https://www.ebi.ac.uk/pdbsum/3l27 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3l27 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3l27 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3l27 OCA], [https://pdbe.org/3l27 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3l27 RCSB], [https://www.ebi.ac.uk/pdbsum/3l27 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3l27 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/VP35_EBOZM VP35_EBOZM]] Acsts as a polymerase cofactor in the RNA polymerase transcription and replication complex. Prevents establishment of cellular antiviral state by blocking virus-induced phosphorylation and activation of interferon regulatory factor 3 (IRF3), a transcription factor critical for the induction of interferons alpha and beta. The mechanism by which this blockage occurs remains incompletely defined, a hypothesis suggests that VP35 dsRNA-binding activity prevents activation of IRF3 by sequestering dsRNA. Also inhibits the antiviral effect mediated by the interferon-induced, double-stranded RNA-activated protein kinase EIF2AK2/PKR.<ref>PMID:9971816</ref> <ref>PMID:11027311</ref> <ref>PMID:12829834</ref> <ref>PMID:16495261</ref> <ref>PMID:17065211</ref>	+	[https://www.uniprot.org/uniprot/VP35_EBOZM VP35_EBOZM] Acsts as a polymerase cofactor in the RNA polymerase transcription and replication complex. Prevents establishment of cellular antiviral state by blocking virus-induced phosphorylation and activation of interferon regulatory factor 3 (IRF3), a transcription factor critical for the induction of interferons alpha and beta. The mechanism by which this blockage occurs remains incompletely defined, a hypothesis suggests that VP35 dsRNA-binding activity prevents activation of IRF3 by sequestering dsRNA. Also inhibits the antiviral effect mediated by the interferon-induced, double-stranded RNA-activated protein kinase EIF2AK2/PKR.<ref>PMID:9971816</ref> <ref>PMID:11027311</ref> <ref>PMID:12829834</ref> <ref>PMID:16495261</ref> <ref>PMID:17065211</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Ebov~~-~~may~~]]	+	[[Category: Ebola virus - Mayinga, Zaire, 1976]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Amarasinghe~~, G K~~]]	+	[[Category: Amarasinghe GK]]
-	[[Category: Basler~~, C F~~]]	+	[[Category: Basler CF]]
-	[[Category: Borek~~, D M~~]]	+	[[Category: Borek DM]]
-	[[Category: Farahbakhsh, M]]	+	[[Category: Farahbakhsh M]]
-	[[Category: Helgeson~~, L A~~]]	+	[[Category: Helgeson LA]]
-	[[Category: Honzatko~~, R B~~]]	+	[[Category: Honzatko RB]]
-	[[Category: Leung~~, D W~~]]	+	[[Category: Leung DW]]
-	[[Category: Nix~~, J C~~]]	+	[[Category: Nix JC]]
-	[[Category: Otwinowski, Z]]	+	[[Category: Otwinowski Z]]
-	[[Category: Prins~~, K C~~]]	+	[[Category: Prins KC]]
-	[[Category: Ramanan, P]]	+	[[Category: Ramanan P]]
-	[[Category: Tufariello~~, J M]]~~	+	[[Category: Tufariello JM]]
-	~~[[Category: Host cytoplasm]]~~	+
-	~~[[Category: Interferon antiviral evasion]]~~	+
-	~~[[Category: Interferon antiviral system evasion]]~~	+
-	~~[[Category: Rna binding domain]]~~	+
-	~~[[Category: Rna binding protein]]~~	+
-	~~[[Category: Rna replication]]~~	+
-	~~[[Category: Rna-binding]]~~	+
-	~~[[Category: Rna-binding protein]]~~	+
-	~~[[Category: Transcription]]~~	+
-	~~[[Category: Virion~~]]	+

OCA at 12:31, 13 October 2021

OCA — Wed, 13 Oct 2021 12:31:11 GMT

←Older revision		Revision as of 12:31, 13 October 2021
Line 1:		Line 1:

	==Crystal structure of Zaire Ebola VP35 interferon inhibitory domain R312A mutant==		==Crystal structure of Zaire Ebola VP35 interferon inhibitory domain R312A mutant==
-	<StructureSection load='3l27' size='340' side='right' caption='[[3l27]], [[Resolution\|resolution]] 1.95Å' scene=''>	+	<StructureSection load='3l27' size='340' side='right'caption='[[3l27]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3l27]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Ebov-may Ebov-may]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L27 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3L27 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3l27]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Ebov-may Ebov-may]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L27 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3L27 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3fke\|3fke]], [[3l25\|3l25]], [[3l26\|3l26]], [[3l28\|3l28]], [[3l29\|3l29]], [[3l2a\|3l2a]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3fke\|3fke]], [[3l25\|3l25]], [[3l26\|3l26]], [[3l28\|3l28]], [[3l29\|3l29]], [[3l2a\|3l2a]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">VP35 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=128952 EBOV-May])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">VP35 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=128952 EBOV-May])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3l27 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3l27 OCA], [~~http~~://pdbe.org/3l27 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3l27 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3l27 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3l27 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3l27 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3l27 OCA], [https://pdbe.org/3l27 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3l27 RCSB], [https://www.ebi.ac.uk/pdbsum/3l27 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3l27 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/VP35_EBOZM VP35_EBOZM]] Acsts as a polymerase cofactor in the RNA polymerase transcription and replication complex. Prevents establishment of cellular antiviral state by blocking virus-induced phosphorylation and activation of interferon regulatory factor 3 (IRF3), a transcription factor critical for the induction of interferons alpha and beta. The mechanism by which this blockage occurs remains incompletely defined, a hypothesis suggests that VP35 dsRNA-binding activity prevents activation of IRF3 by sequestering dsRNA. Also inhibits the antiviral effect mediated by the interferon-induced, double-stranded RNA-activated protein kinase EIF2AK2/PKR.<ref>PMID:9971816</ref> <ref>PMID:11027311</ref> <ref>PMID:12829834</ref> <ref>PMID:16495261</ref> <ref>PMID:17065211</ref>	+	[[https://www.uniprot.org/uniprot/VP35_EBOZM VP35_EBOZM]] Acsts as a polymerase cofactor in the RNA polymerase transcription and replication complex. Prevents establishment of cellular antiviral state by blocking virus-induced phosphorylation and activation of interferon regulatory factor 3 (IRF3), a transcription factor critical for the induction of interferons alpha and beta. The mechanism by which this blockage occurs remains incompletely defined, a hypothesis suggests that VP35 dsRNA-binding activity prevents activation of IRF3 by sequestering dsRNA. Also inhibits the antiviral effect mediated by the interferon-induced, double-stranded RNA-activated protein kinase EIF2AK2/PKR.<ref>PMID:9971816</ref> <ref>PMID:11027311</ref> <ref>PMID:12829834</ref> <ref>PMID:16495261</ref> <ref>PMID:17065211</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 25:
	</StructureSection>		</StructureSection>
	[[Category: Ebov-may]]		[[Category: Ebov-may]]
		+	[[Category: Large Structures]]
	[[Category: Amarasinghe, G K]]		[[Category: Amarasinghe, G K]]
	[[Category: Basler, C F]]		[[Category: Basler, C F]]

OCA at 06:38, 2 January 2019

OCA — Wed, 02 Jan 2019 06:38:00 GMT

←Older revision		Revision as of 06:38, 2 January 2019
Line 3:		Line 3:
	<StructureSection load='3l27' size='340' side='right' caption='[[3l27]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='3l27' size='340' side='right' caption='[[3l27]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3l27]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Ebozm Ebozm~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L27 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3L27 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3l27]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Ebov-may Ebov-may]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L27 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3L27 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3fke\|3fke]], [[3l25\|3l25]], [[3l26\|3l26]], [[3l28\|3l28]], [[3l29\|3l29]], [[3l2a\|3l2a]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3fke\|3fke]], [[3l25\|3l25]], [[3l26\|3l26]], [[3l28\|3l28]], [[3l29\|3l29]], [[3l2a\|3l2a]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">VP35 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=128952 ~~EBOZM~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">VP35 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=128952 EBOV-May])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3l27 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3l27 OCA], [http://pdbe.org/3l27 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3l27 RCSB], [http://www.ebi.ac.uk/pdbsum/3l27 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3l27 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3l27 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3l27 OCA], [http://pdbe.org/3l27 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3l27 RCSB], [http://www.ebi.ac.uk/pdbsum/3l27 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3l27 ProSAT]</span></td></tr>
	</table>		</table>
Line 24:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Ebozm~~]]	+	[[Category: Ebov-may]]
	[[Category: Amarasinghe, G K]]		[[Category: Amarasinghe, G K]]
	[[Category: Basler, C F]]		[[Category: Basler, C F]]

OCA at 19:09, 4 August 2016

OCA — Thu, 04 Aug 2016 19:09:54 GMT

←Older revision		Revision as of 19:09, 4 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of Zaire Ebola VP35 interferon inhibitory domain R312A mutant==		==Crystal structure of Zaire Ebola VP35 interferon inhibitory domain R312A mutant==
	<StructureSection load='3l27' size='340' side='right' caption='[[3l27]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='3l27' size='340' side='right' caption='[[3l27]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3l27]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Zaire_ebolavirus Zaire ebolavirus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L27 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3L27 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3l27]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Ebozm Ebozm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L27 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3L27 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3fke\|3fke]], [[3l25\|3l25]], [[3l26\|3l26]], [[3l28\|3l28]], [[3l29\|3l29]], [[3l2a\|3l2a]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3fke\|3fke]], [[3l25\|3l25]], [[3l26\|3l26]], [[3l28\|3l28]], [[3l29\|3l29]], [[3l2a\|3l2a]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">VP35 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~186538 Zaire ebolavirus~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">VP35 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=128952 EBOZM])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3l27 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3l27 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3l27 RCSB], [http://www.ebi.ac.uk/pdbsum/3l27 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3l27 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3l27 OCA], [http://pdbe.org/3l27 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3l27 RCSB], [http://www.ebi.ac.uk/pdbsum/3l27 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3l27 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3l27" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Zaire ebolavirus~~]]	+	[[Category: Ebozm]]
	[[Category: Amarasinghe, G K]]		[[Category: Amarasinghe, G K]]
	[[Category: Basler, C F]]		[[Category: Basler, C F]]

OCA at 18:34, 24 December 2014

OCA — Wed, 24 Dec 2014 18:34:16 GMT

←Older revision		Revision as of 18:34, 24 December 2014
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3l27 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3l27 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3l27 RCSB], [http://www.ebi.ac.uk/pdbsum/3l27 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3l27 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3l27 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3l27 RCSB], [http://www.ebi.ac.uk/pdbsum/3l27 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/VP35_EBOZM VP35_EBOZM]] Acsts as a polymerase cofactor in the RNA polymerase transcription and replication complex. Prevents establishment of cellular antiviral state by blocking virus-induced phosphorylation and activation of interferon regulatory factor 3 (IRF3), a transcription factor critical for the induction of interferons alpha and beta. The mechanism by which this blockage occurs remains incompletely defined, a hypothesis suggests that VP35 dsRNA-binding activity prevents activation of IRF3 by sequestering dsRNA. Also inhibits the antiviral effect mediated by the interferon-induced, double-stranded RNA-activated protein kinase EIF2AK2/PKR.<ref>PMID:9971816</ref> <ref>PMID:11027311</ref> <ref>PMID:12829834</ref> <ref>PMID:16495261</ref> <ref>PMID:17065211</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 16:41, 18 December 2014

OCA — Thu, 18 Dec 2014 16:41:17 GMT

←Older revision		Revision as of 16:41, 18 December 2014
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-	~~{{STRUCTURE_3l27\| PDB=3l27 \| SCENE= }}~~	+	==Crystal structure of Zaire Ebola VP35 interferon inhibitory domain R312A mutant==
-	===Crystal structure of Zaire Ebola VP35 interferon inhibitory domain R312A mutant===	+	<StructureSection load='3l27' size='340' side='right' caption='[[3l27]], [[Resolution\|resolution]] 1.95Å' scene=''>
-	~~{{ABSTRACT_PUBMED_20081868}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3l27]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Zaire_ebolavirus Zaire ebolavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L27 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3L27 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3fke\|3fke]], [[3l25\|3l25]], [[3l26\|3l26]], [[3l28\|3l28]], [[3l29\|3l29]], [[3l2a\|3l2a]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">VP35 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=186538 Zaire ebolavirus])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3l27 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3l27 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3l27 RCSB], [http://www.ebi.ac.uk/pdbsum/3l27 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Ebola viral protein 35 (VP35), encoded by the highly pathogenic Ebola virus, facilitates host immune evasion by antagonizing antiviral signaling pathways, including those initiated by RIG-I-like receptors. Here we report the crystal structure of the Ebola VP35 interferon inhibitory domain (IID) bound to short double-stranded RNA (dsRNA), which together with in vivo results reveals how VP35-dsRNA interactions contribute to immune evasion. Conserved basic residues in VP35 IID recognize the dsRNA backbone, whereas the dsRNA blunt ends are 'end-capped' by a pocket of hydrophobic residues that mimic RIG-I-like receptor recognition of blunt-end dsRNA. Residues critical for RNA binding are also important for interferon inhibition in vivo but not for viral polymerase cofactor function of VP35. These results suggest that simultaneous recognition of dsRNA backbone and blunt ends provides a mechanism by which Ebola VP35 antagonizes host dsRNA sensors and immune responses.

-	~~==Function==~~	+	Structural basis for dsRNA recognition and interferon antagonism by Ebola VP35.,Leung DW, Prins KC, Borek DM, Farahbakhsh M, Tufariello JM, Ramanan P, Nix JC, Helgeson LA, Otwinowski Z, Honzatko RB, Basler CF, Amarasinghe GK Nat Struct Mol Biol. 2010 Feb;17(2):165-72. Epub 2010 Jan 17. PMID:20081868<ref>PMID:20081868</ref>
-	[[http://www.uniprot.org/uniprot/VP35_EBOZM VP35_EBOZM]] Acsts as a polymerase cofactor in the RNA polymerase transcription and replication complex. Prevents establishment of cellular antiviral state by blocking virus-induced phosphorylation and ~~activation of~~ interferon ~~regulatory factor 3 (IRF3), a transcription factor critical for the induction of interferons alpha and beta. The mechanism~~ by ~~which this blockage occurs remains incompletely defined, a hypothesis suggests that~~ VP35 ~~dsRNA-binding activity prevents activation of IRF3 by sequestering dsRNA~~. ~~Also inhibits the antiviral effect mediated by the interferon-induced~~, ~~double~~-~~stranded RNA-activated protein kinase EIF2AK2/PKR~~.~~<ref>~~PMID:~~9971816</ref> <ref>PMID:11027311</ref> <ref>PMID:12829834</ref> <ref>PMID:16495261</ref>~~ <ref>PMID:~~17065211~~</ref>	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[3l27]] is~~ a ~~4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Zaire_ebolavirus Zaire ebolavirus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3L27 OCA].~~	+	</div>
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:020081868</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Zaire ebolavirus]]		[[Category: Zaire ebolavirus]]
-	[[Category: Amarasinghe, G K.]]	+	[[Category: Amarasinghe, G K]]
-	[[Category: Basler, C F.]]	+	[[Category: Basler, C F]]
-	[[Category: Borek, D M.]]	+	[[Category: Borek, D M]]
-	[[Category: Farahbakhsh, M.]]	+	[[Category: Farahbakhsh, M]]
-	[[Category: Helgeson, L A.]]	+	[[Category: Helgeson, L A]]
-	[[Category: Honzatko, R B.]]	+	[[Category: Honzatko, R B]]
-	[[Category: Leung, D W.]]	+	[[Category: Leung, D W]]
-	[[Category: Nix, J C.]]	+	[[Category: Nix, J C]]
-	[[Category: Otwinowski, Z.]]	+	[[Category: Otwinowski, Z]]
-	[[Category: Prins, K C.]]	+	[[Category: Prins, K C]]
-	[[Category: Ramanan, P.]]	+	[[Category: Ramanan, P]]
-	[[Category: Tufariello, J M.]]	+	[[Category: Tufariello, J M]]
	[[Category: Host cytoplasm]]		[[Category: Host cytoplasm]]
	[[Category: Interferon antiviral evasion]]		[[Category: Interferon antiviral evasion]]

OCA: Protected "3l27" [edit=sysop:move=sysop]

OCA — Thu, 04 Apr 2013 20:10:10 GMT

Protected "3l27" [edit=sysop:move=sysop]

←Older revision

Revision as of 20:10, 4 April 2013

OCA at 20:10, 4 April 2013

OCA — Thu, 04 Apr 2013 20:10:05 GMT

←Older revision		Revision as of 20:10, 4 April 2013
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-	{{Seed}}
-	[[Image:3l27.png\|left\|200px]]
-
-	<!--
-	The line below this paragraph, containing "STRUCTURE_3l27", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3l27\| PDB=3l27 \| SCENE= }}		{{STRUCTURE_3l27\| PDB=3l27 \| SCENE= }}
-
	===Crystal structure of Zaire Ebola VP35 interferon inhibitory domain R312A mutant===		===Crystal structure of Zaire Ebola VP35 interferon inhibitory domain R312A mutant===
		+	{{ABSTRACT_PUBMED_20081868}}

-		+	==Function==
-	~~<!--~~	+	[[http://www.uniprot.org/uniprot/VP35_EBOZM VP35_EBOZM]] Acsts as a polymerase cofactor in the RNA polymerase transcription and replication complex. Prevents establishment of cellular antiviral state by blocking virus-induced phosphorylation and activation of interferon regulatory factor 3 (IRF3), a transcription factor critical for the induction of interferons alpha and beta. The mechanism by which this blockage occurs remains incompletely defined, a hypothesis suggests that VP35 dsRNA-binding activity prevents activation of IRF3 by sequestering dsRNA. Also inhibits the antiviral effect mediated by the interferon-induced, double-stranded RNA-activated protein kinase EIF2AK2/PKR.<ref>PMID:9971816</ref> <ref>PMID:11027311</ref> <ref>PMID:12829834</ref> <ref>PMID:16495261</ref> <ref>PMID:17065211</ref>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_20081868}}, adds the Publication Abstract to the page~~	+
-	~~(as it appears on PubMed at~~ http://www.~~pubmed~~.~~gov~~), ~~where 20081868 is~~ the ~~PubMed ID number~~.	+
-	-->	+
-	~~{{ABSTRACT_PUBMED_20081868}}~~	+

	==About this Structure==		==About this Structure==
-	~~3L27~~ is a 4 ~~chains~~ structure with ~~sequences~~ from [http://en.wikipedia.org/wiki/Zaire_ebolavirus Zaire ebolavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L27 OCA].	+	[[3l27]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Zaire_ebolavirus Zaire ebolavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L27 OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~20081868~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:020081868</ref><references group="xtra"/><references/>
	[[Category: Zaire ebolavirus]]		[[Category: Zaire ebolavirus]]
	[[Category: Amarasinghe, G K.]]		[[Category: Amarasinghe, G K.]]
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	[[Category: Ramanan, P.]]		[[Category: Ramanan, P.]]
	[[Category: Tufariello, J M.]]		[[Category: Tufariello, J M.]]
-	[[Category: Coiled coil]]
	[[Category: Host cytoplasm]]		[[Category: Host cytoplasm]]
	[[Category: Interferon antiviral evasion]]		[[Category: Interferon antiviral evasion]]
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	[[Category: Transcription]]		[[Category: Transcription]]
	[[Category: Virion]]		[[Category: Virion]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 17 09:54:05 2010''

OCA at 07:54, 17 February 2010

OCA — Wed, 17 Feb 2010 07:54:06 GMT

←Older revision		Revision as of 07:54, 17 February 2010
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	{{Seed}}		{{Seed}}
-	[[Image:3l27.~~jpg~~\|left\|200px]]	+	[[Image:3l27.png\|left\|200px]]

	<!--		<!--
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	===Crystal structure of Zaire Ebola VP35 interferon inhibitory domain R312A mutant===		===Crystal structure of Zaire Ebola VP35 interferon inhibitory domain R312A mutant===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_20081868}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 20081868 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_20081868}}

	==About this Structure==		==About this Structure==
	3L27 is a 4 chains structure with sequences from [http://en.wikipedia.org/wiki/Zaire_ebolavirus Zaire ebolavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L27 OCA].		3L27 is a 4 chains structure with sequences from [http://en.wikipedia.org/wiki/Zaire_ebolavirus Zaire ebolavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L27 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:20081868</ref><references group="xtra"/>
	[[Category: Zaire ebolavirus]]		[[Category: Zaire ebolavirus]]
	[[Category: Amarasinghe, G K.]]		[[Category: Amarasinghe, G K.]]
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	[[Category: Interferon antiviral system evasion]]		[[Category: Interferon antiviral system evasion]]
	[[Category: Rna binding domain]]		[[Category: Rna binding domain]]
		+	[[Category: Rna binding protein]]
	[[Category: Rna replication]]		[[Category: Rna replication]]
	[[Category: Rna-binding]]		[[Category: Rna-binding]]
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	[[Category: Virion]]		[[Category: Virion]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Jan 27 20~~:06:55 2010''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 17 09:54:05 2010''

OCA at 18:06, 27 January 2010

OCA — Wed, 27 Jan 2010 18:06:56 GMT

←Older revision		Revision as of 18:06, 27 January 2010
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-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3l27.jpg\|left\|200px]]

-	The ~~entry 3l27~~ is ~~ON HOLD~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3l27", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3l27\| PDB=3l27 \| SCENE= }}

-	~~Authors: Leung, D.W., Prins, K.C., Borek, D.M., Farahbakhsh, M., Tufariello, J.M., Ramanan, P., Nix, J.C., Helgeson, L.A., Otwinowski, Z., Honzatko, R.B., Basler, C.F., Amarasinghe, G.K.~~	+	===Crystal structure of Zaire Ebola VP35 interferon inhibitory domain R312A mutant===

-	Description:

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan ~~13 13~~:33:31 2010''	+	==About this Structure==
		+	3L27 is a 4 chains structure with sequences from [http://en.wikipedia.org/wiki/Zaire_ebolavirus Zaire ebolavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L27 OCA].
		+	[[Category: Zaire ebolavirus]]
		+	[[Category: Amarasinghe, G K.]]
		+	[[Category: Basler, C F.]]
		+	[[Category: Borek, D M.]]
		+	[[Category: Farahbakhsh, M.]]
		+	[[Category: Helgeson, L A.]]
		+	[[Category: Honzatko, R B.]]
		+	[[Category: Leung, D W.]]
		+	[[Category: Nix, J C.]]
		+	[[Category: Otwinowski, Z.]]
		+	[[Category: Prins, K C.]]
		+	[[Category: Ramanan, P.]]
		+	[[Category: Tufariello, J M.]]
		+	[[Category: Coiled coil]]
		+	[[Category: Host cytoplasm]]
		+	[[Category: Interferon antiviral evasion]]
		+	[[Category: Interferon antiviral system evasion]]
		+	[[Category: Rna binding domain]]
		+	[[Category: Rna replication]]
		+	[[Category: Rna-binding]]
		+	[[Category: Rna-binding protein]]
		+	[[Category: Transcription]]
		+	[[Category: Virion]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 27 20:06:55 2010''