3lat - Revision history

OCA at 08:30, 20 March 2024

OCA — Wed, 20 Mar 2024 08:30:40 GMT

←Older revision		Revision as of 08:30, 20 March 2024
Line 1:		Line 1:

	==Crystal structure of Staphylococcus peptidoglycan hydrolase AmiE==		==Crystal structure of Staphylococcus peptidoglycan hydrolase AmiE==
-	<StructureSection load='3lat' size='340' side='right' caption='[[3lat]], [[Resolution\|resolution]] 1.70Å' scene=''>	+	<StructureSection load='3lat' size='340' side='right'caption='[[3lat]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3lat]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"albococcus_epidermidis"_winslow_and_winslow_1908 "albococcus~~ epidermidis~~" winslow and winslow 1908~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LAT OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3LAT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3lat]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_epidermidis Staphylococcus epidermidis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LAT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3LAT FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BU1:1,4-BUTANEDIOL'>BU1</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">atlE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1282 "Albococcus epidermidis" Winslow and Winslow 1908])</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BU1:1,4-BUTANEDIOL'>BU1</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/N-acetylmuramoyl-L-alanine_amidase N-acetylmuramoyl-L-alanine amidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.28 3.5.1.28] </span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3lat FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3lat OCA], [https://pdbe.org/3lat PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3lat RCSB], [https://www.ebi.ac.uk/pdbsum/3lat PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3lat ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3lat FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3lat OCA], [~~http~~://pdbe.org/3lat PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3lat RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3lat PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3lat ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/ATL_STAEP ATL_STAEP]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 19:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3lat ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3lat ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The major autolysins (Atl) of Staphylococcus epidermidis and S. aureus play an important role in cell separation, and their mutants are also attenuated in virulence. Therefore, autolysins represent a promising target for the development of new types of antibiotics. Here, we report the high-resolution structure of the catalytically active amidase domain AmiE (amidase S. epidermidis) from the major autolysin of S. epidermidis. This is the first protein structure with an amidase-like fold from a bacterium with a gram-positive cell wall architecture. AmiE adopts a globular fold, with several alpha-helices surrounding a central beta-sheet. Sequence comparison reveals a cluster of conserved amino acids that define a putative binding site with a buried zinc ion. Mutations of key residues in the putative active site result in loss of activity, enabling us to propose a catalytic mechanism. We also identified and synthesized muramyltripeptide, the minimal peptidoglycan fragment that can be used as a substrate by the enzyme. Molecular docking and digestion assays with muramyltripeptide derivatives allow us to identify key determinants of ligand binding. This results in a plausible model of interaction of this ligand not only for AmiE, but also for other PGN-hydrolases that share the same fold. As AmiE active-site mutations also show a severe growth defect, our findings provide an excellent platform for the design of specific inhibitors that target staphylococcal cell separation and can thereby prevent growth of this pathogen.
-
-	Structural basis of cell wall cleavage by a staphylococcal autolysin.,Zoll S, Patzold B, Schlag M, Gotz F, Kalbacher H, Stehle T PLoS Pathog. 2010 Mar 12;6(3):e1000807. PMID:20300605<ref>PMID:20300605</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3lat" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Albococcus epidermidis winslow and winslow 1908~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~N-acetylmuramoyl-L-alanine amidase~~]]	+	[[Category: Staphylococcus epidermidis]]
-	[[Category: Stehle, T]]	+	[[Category: Stehle T]]
-	[[Category: Zoll, S]]	+	[[Category: Zoll S]]
-	~~[[Category: Amidase]]~~	+
-	~~[[Category: Atle]]~~	+
-	~~[[Category: Autolysin]]~~	+
-	~~[[Category: Cell wall biogenesis/degradation]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Multifunctional enzyme]]~~	+
-	~~[[Category: Peptidoglycan hydrolase]]~~	+
-	~~[[Category: Secreted~~]]	+

OCA at 07:18, 2 January 2019

OCA — Wed, 02 Jan 2019 07:18:14 GMT

←Older revision		Revision as of 07:18, 2 January 2019
Line 13:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/la/3lat_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/la/3lat_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 14:22, 4 August 2016

OCA — Thu, 04 Aug 2016 14:22:53 GMT

←Older revision		Revision as of 14:22, 4 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of Staphylococcus peptidoglycan hydrolase AmiE==		==Crystal structure of Staphylococcus peptidoglycan hydrolase AmiE==
	<StructureSection load='3lat' size='340' side='right' caption='[[3lat]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='3lat' size='340' side='right' caption='[[3lat]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3lat]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Staphylococcus_epidermidis Staphylococcus~~ epidermidis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LAT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3LAT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3lat]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"albococcus_epidermidis"_winslow_and_winslow_1908 "albococcus epidermidis" winslow and winslow 1908]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LAT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3LAT FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BU1:1,4-BUTANEDIOL'>BU1</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BU1:1,4-BUTANEDIOL'>BU1</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">atlE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1282 ~~Staphylococcus~~ epidermidis])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">atlE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1282 "Albococcus epidermidis" Winslow and Winslow 1908])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/N-acetylmuramoyl-L-alanine_amidase N-acetylmuramoyl-L-alanine amidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.28 3.5.1.28] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/N-acetylmuramoyl-L-alanine_amidase N-acetylmuramoyl-L-alanine amidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.28 3.5.1.28] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3lat FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3lat OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3lat RCSB], [http://www.ebi.ac.uk/pdbsum/3lat PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3lat FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3lat OCA], [http://pdbe.org/3lat PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3lat RCSB], [http://www.ebi.ac.uk/pdbsum/3lat PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3lat ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 16:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3lat ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 26:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3lat" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Albococcus epidermidis winslow and winslow 1908]]
	[[Category: N-acetylmuramoyl-L-alanine amidase]]		[[Category: N-acetylmuramoyl-L-alanine amidase]]
-	[[Category: Staphylococcus epidermidis]]
	[[Category: Stehle, T]]		[[Category: Stehle, T]]
	[[Category: Zoll, S]]		[[Category: Zoll, S]]

OCA at 16:28, 18 December 2014

OCA — Thu, 18 Dec 2014 16:28:28 GMT

←Older revision		Revision as of 16:28, 18 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3lat~~\| ~~PDB~~=3lat \| ~~SCENE~~= }}	+	==Crystal structure of Staphylococcus peptidoglycan hydrolase AmiE==
-	===~~Crystal~~ structure of ~~Staphylococcus peptidoglycan hydrolase AmiE~~===	+	<StructureSection load='3lat' size='340' side='right' caption='[[3lat]], [[Resolution\|resolution]] 1.70Å' scene=''>
-	~~{{ABSTRACT_PUBMED_20300605}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3lat]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_epidermidis Staphylococcus epidermidis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LAT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3LAT FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BU1:1,4-BUTANEDIOL'>BU1</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">atlE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1282 Staphylococcus epidermidis])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/N-acetylmuramoyl-L-alanine_amidase N-acetylmuramoyl-L-alanine amidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.28 3.5.1.28] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3lat FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3lat OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3lat RCSB], [http://www.ebi.ac.uk/pdbsum/3lat PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/la/3lat_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The major autolysins (Atl) of Staphylococcus epidermidis and S. aureus play an important role in cell separation, and their mutants are also attenuated in virulence. Therefore, autolysins represent a promising target for the development of new types of antibiotics. Here, we report the high-resolution structure of the catalytically active amidase domain AmiE (amidase S. epidermidis) from the major autolysin of S. epidermidis. This is the first protein structure with an amidase-like fold from a bacterium with a gram-positive cell wall architecture. AmiE adopts a globular fold, with several alpha-helices surrounding a central beta-sheet. Sequence comparison reveals a cluster of conserved amino acids that define a putative binding site with a buried zinc ion. Mutations of key residues in the putative active site result in loss of activity, enabling us to propose a catalytic mechanism. We also identified and synthesized muramyltripeptide, the minimal peptidoglycan fragment that can be used as a substrate by the enzyme. Molecular docking and digestion assays with muramyltripeptide derivatives allow us to identify key determinants of ligand binding. This results in a plausible model of interaction of this ligand not only for AmiE, but also for other PGN-hydrolases that share the same fold. As AmiE active-site mutations also show a severe growth defect, our findings provide an excellent platform for the design of specific inhibitors that target staphylococcal cell separation and can thereby prevent growth of this pathogen.

-	~~==About this Structure==~~	+	Structural basis of cell wall cleavage by a staphylococcal autolysin.,Zoll S, Patzold B, Schlag M, Gotz F, Kalbacher H, Stehle T PLoS Pathog. 2010 Mar 12;6(3):e1000807. PMID:20300605<ref>PMID:20300605</ref>
-	~~[[3lat]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Staphylococcus_epidermidis Staphylococcus epidermidis]~~. ~~Full crystallographic information is available from [http~~:/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3LAT OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:020300605</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: N-acetylmuramoyl-L-alanine amidase]]		[[Category: N-acetylmuramoyl-L-alanine amidase]]
	[[Category: Staphylococcus epidermidis]]		[[Category: Staphylococcus epidermidis]]
-	[[Category: Stehle, T.]]	+	[[Category: Stehle, T]]
-	[[Category: Zoll, S.]]	+	[[Category: Zoll, S]]
	[[Category: Amidase]]		[[Category: Amidase]]
	[[Category: Atle]]		[[Category: Atle]]

OCA: Protected "3lat" [edit=sysop:move=sysop]

OCA — Thu, 04 Apr 2013 20:39:58 GMT

Protected "3lat" [edit=sysop:move=sysop]

←Older revision

Revision as of 20:39, 4 April 2013

OCA at 20:39, 4 April 2013

OCA — Thu, 04 Apr 2013 20:39:56 GMT

←Older revision		Revision as of 20:39, 4 April 2013
Line 1:		Line 1:
-	{{Seed}}
-	[[Image:3lat.png\|left\|200px]]
-
-	<!--
-	The line below this paragraph, containing "STRUCTURE_3lat", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3lat\| PDB=3lat \| SCENE= }}		{{STRUCTURE_3lat\| PDB=3lat \| SCENE= }}
-
	===Crystal structure of Staphylococcus peptidoglycan hydrolase AmiE===		===Crystal structure of Staphylococcus peptidoglycan hydrolase AmiE===
-
-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_20300605}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 20300605 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_20300605}}		{{ABSTRACT_PUBMED_20300605}}

	==About this Structure==		==About this Structure==
-	~~3LAT~~ is a 2 ~~chains~~ structure with ~~sequences~~ from [http://en.wikipedia.org/wiki/Staphylococcus_epidermidis Staphylococcus epidermidis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LAT OCA].	+	[[3lat]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_epidermidis Staphylococcus epidermidis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LAT OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~20300605~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:020300605</ref><references group="xtra"/><references/>
	[[Category: N-acetylmuramoyl-L-alanine amidase]]		[[Category: N-acetylmuramoyl-L-alanine amidase]]
	[[Category: Staphylococcus epidermidis]]		[[Category: Staphylococcus epidermidis]]
Line 36:		Line 20:
	[[Category: Peptidoglycan hydrolase]]		[[Category: Peptidoglycan hydrolase]]
	[[Category: Secreted]]		[[Category: Secreted]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 31 12:26:00 2010''

OCA at 09:26, 31 March 2010

OCA — Wed, 31 Mar 2010 09:26:01 GMT

←Older revision		Revision as of 09:26, 31 March 2010
Line 1:		Line 1:
	{{Seed}}		{{Seed}}
-	[[Image:3lat.~~jpg~~\|left\|200px]]	+	[[Image:3lat.png\|left\|200px]]

	<!--		<!--
Line 12:		Line 12:
	===Crystal structure of Staphylococcus peptidoglycan hydrolase AmiE===		===Crystal structure of Staphylococcus peptidoglycan hydrolase AmiE===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_20300605}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 20300605 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_20300605}}

	==About this Structure==		==About this Structure==
	3LAT is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Staphylococcus_epidermidis Staphylococcus epidermidis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LAT OCA].		3LAT is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Staphylococcus_epidermidis Staphylococcus epidermidis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LAT OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:20300605</ref><references group="xtra"/>
	[[Category: N-acetylmuramoyl-L-alanine amidase]]		[[Category: N-acetylmuramoyl-L-alanine amidase]]
	[[Category: Staphylococcus epidermidis]]		[[Category: Staphylococcus epidermidis]]
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	[[Category: Secreted]]		[[Category: Secreted]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar ~~24 09~~:04:53 2010''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 31 12:26:00 2010''

OCA at 07:04, 24 March 2010

OCA — Wed, 24 Mar 2010 07:04:55 GMT

←Older revision		Revision as of 07:04, 24 March 2010
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3lat.jpg\|left\|200px]]

-	The ~~entry 3lat~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3lat", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3lat\| PDB=3lat \| SCENE= }}

-	~~Authors: Zoll, S., Stehle, T.~~	+	===Crystal structure of Staphylococcus peptidoglycan hydrolase AmiE===

-	Description:

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Feb 3~~ 09:18:36 2010''	+	==About this Structure==
		+	3LAT is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Staphylococcus_epidermidis Staphylococcus epidermidis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LAT OCA].
		+	[[Category: N-acetylmuramoyl-L-alanine amidase]]
		+	[[Category: Staphylococcus epidermidis]]
		+	[[Category: Stehle, T.]]
		+	[[Category: Zoll, S.]]
		+	[[Category: Amidase]]
		+	[[Category: Atle]]
		+	[[Category: Autolysin]]
		+	[[Category: Cell wall biogenesis/degradation]]
		+	[[Category: Hydrolase]]
		+	[[Category: Multifunctional enzyme]]
		+	[[Category: Peptidoglycan hydrolase]]
		+	[[Category: Secreted]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 24 09:04:53 2010''

OCA at 07:18, 3 February 2010

OCA — Wed, 03 Feb 2010 07:18:36 GMT

←Older revision		Revision as of 07:18, 3 February 2010
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3lat is ON HOLD	+	The entry 3lat is ON HOLD until Paper Publication

	Authors: Zoll, S., Stehle, T.		Authors: Zoll, S., Stehle, T.
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	Description:		Description:

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Jan 27 19~~:17:09 2010''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 3 09:18:36 2010''

OCA at 17:17, 27 January 2010

OCA — Wed, 27 Jan 2010 17:17:09 GMT

←Older revision		Revision as of 17:17, 27 January 2010
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	The entry 3lat is ON HOLD		The entry 3lat is ON HOLD

-	Authors: Zoll, S~~., Paetzold, B., Schlag, M., Goetz, F., Kalbacher, H~~., Stehle, T.	+	Authors: Zoll, S., Stehle, T.

-	Description: ~~Crystal structure~~	+	Description:

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan ~~13 13~~:38:05 2010''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 27 19:17:09 2010''