3ldz - Revision history

OCA at 10:20, 21 February 2024

OCA — Wed, 21 Feb 2024 10:20:09 GMT

←Older revision		Revision as of 10:20, 21 February 2024
Line 3:		Line 3:
	<StructureSection load='3ldz' size='340' side='right'caption='[[3ldz]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='3ldz' size='340' side='right'caption='[[3ldz]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3ldz]] is a 7 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [~~http~~://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LDZ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3LDZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3ldz]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LDZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3LDZ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3ldz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ldz OCA], [~~http~~://pdbe.org/3ldz PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3ldz RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3ldz PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3ldz ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.6Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ldz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ldz OCA], [https://pdbe.org/3ldz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ldz RCSB], [https://www.ebi.ac.uk/pdbsum/3ldz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ldz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~STAM1_HUMAN STAM1_HUMAN~~]~~] Involved in intracellular signal transduction mediated by cytokines and growth factors~~. ~~Upon IL-2 and GM-CSL stimulation~~, it ~~plays a role in signaling leading~~ to ~~DNA synthesis and MYC induction~~. ~~May also play~~ a ~~role~~ in T-~~cell development. Involved~~ in ~~down~~-regulation ~~of receptor tyrosine~~ kinase via ~~multivesicular body (MVBs) when complexed with HGS (ESCRT~~-~~0 complex)~~. ~~The ESCRT~~-~~0 complex binds ubiquitin and acts~~ as ~~sorting machinery that recognizes ubiquitinated receptors~~ and ~~transfers them to further sequential lysosomal sorting/trafficking processes~~.	+	[https://www.uniprot.org/uniprot/UBC_HUMAN UBC_HUMAN] Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.<ref>PMID:16543144</ref> <ref>PMID:19754430</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 18:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ldz ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ldz ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	VHS (Vps27, Hrs, and STAM) domains occur in ESCRT-0 subunits Hrs and STAM, GGA adapters, and other trafficking proteins. The structure of the STAM VHS domain-ubiquitin complex was solved at 2.6 A resolution, revealing that determinants for ubiquitin recognition are conserved in nearly all VHS domains. VHS domains from all classes of VHS-domain containing proteins in yeast and humans, including both subunits of ESCRT-0, bound ubiquitin in vitro. ESCRTs have been implicated in the sorting of Lys63-linked polyubiquitinated cargo. Intact human ESCRT-0 binds Lys63-linked tetraubiquitin 50-fold more tightly than monoubiquitin, though only 2-fold more tightly than Lys48-linked tetraubiquitin. The gain in affinity is attributed to the cooperation of flexibly connected VHS and UIM motifs of ESCRT-0 in avid binding to the polyubiquitin chain. Mutational analysis of all the five ubiquitin-binding sites in yeast ESCRT-0 shows that cooperation between them is required for the sorting of the Lys63-linked polyubiquitinated cargo Cps1 to the vacuole.
-
-	VHS domains of ESCRT-0 cooperate in high-avidity binding to polyubiquitinated cargo.,Ren X, Hurley JH EMBO J. 2010 Mar 17;29(6):1045-54. Epub 2010 Feb 11. PMID:20150893<ref>PMID:20150893</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3ldz" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
-	*[[~~Ubiquitin~~\|~~Ubiquitin~~]]	+	*[[3D structures of ubiquitin\|3D structures of ubiquitin]]
	== References ==		== References ==
	<references/>		<references/>
Line 37:		Line 29:
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Hurley~~, J H~~]]	+	[[Category: Hurley JH]]
-	[[Category: Ren, X]]	+	[[Category: Ren X]]
-	~~[[Category: Cytoplasm]]~~	+
-	~~[[Category: Endosome]]~~	+
-	~~[[Category: Membrane]]~~	+
-	~~[[Category: Protein transport]]~~	+
-	~~[[Category: Sh3 domain]]~~	+
-	~~[[Category: Transport]]~~	+
-	~~[[Category: Ubiquitin-binding]]~~	+
-	~~[[Category: Ubl conjugation~~]]	+

OCA at 13:58, 25 December 2019

OCA — Wed, 25 Dec 2019 13:58:25 GMT

←Older revision		Revision as of 13:58, 25 December 2019
Line 1:		Line 1:

	==Crystal structure of human STAM1 VHS domain in complex with ubiquitin==		==Crystal structure of human STAM1 VHS domain in complex with ubiquitin==
-	<StructureSection load='3ldz' size='340' side='right' caption='[[3ldz]], [[Resolution\|resolution]] 2.60Å' scene=''>	+	<StructureSection load='3ldz' size='340' side='right'caption='[[3ldz]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3ldz]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LDZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3LDZ FirstGlance]. <br>		<table><tr><td colspan='2'>[[3ldz]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LDZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3LDZ FirstGlance]. <br>
Line 27:		Line 27:
	</div>		</div>
	<div class="pdbe-citations 3ldz" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3ldz" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Ubiquitin\|Ubiquitin]]
	== References ==		== References ==
	<references/>		<references/>
Line 33:		Line 36:
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
		+	[[Category: Large Structures]]
	[[Category: Hurley, J H]]		[[Category: Hurley, J H]]
	[[Category: Ren, X]]		[[Category: Ren, X]]

OCA at 07:01, 28 February 2018

OCA — Wed, 28 Feb 2018 07:01:33 GMT

←Older revision		Revision as of 07:01, 28 February 2018
Line 12:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ld/3ldz_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ld/3ldz_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 08:54, 1 November 2017

OCA — Wed, 01 Nov 2017 08:54:01 GMT

←Older revision		Revision as of 08:54, 1 November 2017
Line 1:		Line 1:
		+
	==Crystal structure of human STAM1 VHS domain in complex with ubiquitin==		==Crystal structure of human STAM1 VHS domain in complex with ubiquitin==
	<StructureSection load='3ldz' size='340' side='right' caption='[[3ldz]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='3ldz' size='340' side='right' caption='[[3ldz]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3ldz]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LDZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3LDZ FirstGlance]. <br>		<table><tr><td colspan='2'>[[3ldz]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LDZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3LDZ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ldz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ldz OCA], [http://pdbe.org/3ldz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ldz RCSB], [http://www.ebi.ac.uk/pdbsum/3ldz PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ldz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ldz OCA], [http://pdbe.org/3ldz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ldz RCSB], [http://www.ebi.ac.uk/pdbsum/3ldz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ldz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 27:
	</div>		</div>
	<div class="pdbe-citations 3ldz" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3ldz" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Ubiquitin\|Ubiquitin]]
	== References ==		== References ==
	<references/>		<references/>
Line 37:		Line 35:
	[[Category: Hurley, J H]]		[[Category: Hurley, J H]]
	[[Category: Ren, X]]		[[Category: Ren, X]]
		+	[[Category: Cytoplasm]]
	[[Category: Endosome]]		[[Category: Endosome]]
	[[Category: Membrane]]		[[Category: Membrane]]
Line 43:		Line 42:
	[[Category: Transport]]		[[Category: Transport]]
	[[Category: Ubiquitin-binding]]		[[Category: Ubiquitin-binding]]
		+	[[Category: Ubl conjugation]]

OCA at 01:07, 8 February 2016

OCA — Mon, 08 Feb 2016 01:07:05 GMT

←Older revision		Revision as of 01:07, 8 February 2016
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3ldz]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LDZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3LDZ FirstGlance]. <br>		<table><tr><td colspan='2'>[[3ldz]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LDZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3LDZ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ldz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ldz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ldz RCSB], [http://www.ebi.ac.uk/pdbsum/3ldz PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ldz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ldz OCA], [http://pdbe.org/3ldz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ldz RCSB], [http://www.ebi.ac.uk/pdbsum/3ldz PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 15:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ldz ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3ldz" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 19:27, 24 December 2014

OCA — Wed, 24 Dec 2014 19:27:42 GMT

←Older revision		Revision as of 19:27, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3ldz]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LDZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3LDZ FirstGlance]. <br>		<table><tr><td colspan='2'>[[3ldz]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LDZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3LDZ FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ldz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ldz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ldz RCSB], [http://www.ebi.ac.uk/pdbsum/3ldz PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ldz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ldz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ldz RCSB], [http://www.ebi.ac.uk/pdbsum/3ldz PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/STAM1_HUMAN STAM1_HUMAN]] Involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. May also play a role in T-cell development. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with HGS (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 34:
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Hurley, J H.]]	+	[[Category: Hurley, J H]]
-	[[Category: Ren, X.]]	+	[[Category: Ren, X]]
	[[Category: Endosome]]		[[Category: Endosome]]
	[[Category: Membrane]]		[[Category: Membrane]]

OCA at 13:17, 29 September 2014

OCA — Mon, 29 Sep 2014 13:17:35 GMT

←Older revision		Revision as of 13:17, 29 September 2014
Line 1:		Line 1:
-	[[~~Image~~:3ldz.~~png\|left~~\|200px]]	+	==Crystal structure of human STAM1 VHS domain in complex with ubiquitin==
		+	<StructureSection load='3ldz' size='340' side='right' caption='[[3ldz]], [[Resolution\|resolution]] 2.60Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3ldz]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LDZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3LDZ FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ldz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ldz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ldz RCSB], [http://www.ebi.ac.uk/pdbsum/3ldz PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ld/3ldz_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	VHS (Vps27, Hrs, and STAM) domains occur in ESCRT-0 subunits Hrs and STAM, GGA adapters, and other trafficking proteins. The structure of the STAM VHS domain-ubiquitin complex was solved at 2.6 A resolution, revealing that determinants for ubiquitin recognition are conserved in nearly all VHS domains. VHS domains from all classes of VHS-domain containing proteins in yeast and humans, including both subunits of ESCRT-0, bound ubiquitin in vitro. ESCRTs have been implicated in the sorting of Lys63-linked polyubiquitinated cargo. Intact human ESCRT-0 binds Lys63-linked tetraubiquitin 50-fold more tightly than monoubiquitin, though only 2-fold more tightly than Lys48-linked tetraubiquitin. The gain in affinity is attributed to the cooperation of flexibly connected VHS and UIM motifs of ESCRT-0 in avid binding to the polyubiquitin chain. Mutational analysis of all the five ubiquitin-binding sites in yeast ESCRT-0 shows that cooperation between them is required for the sorting of the Lys63-linked polyubiquitinated cargo Cps1 to the vacuole.

-	~~{{STRUCTURE_3ldz\| PDB=3ldz \| SCENE= }}~~	+	VHS domains of ESCRT-0 cooperate in high-avidity binding to polyubiquitinated cargo.,Ren X, Hurley JH EMBO J. 2010 Mar 17;29(6):1045-54. Epub 2010 Feb 11. PMID:20150893<ref>PMID:20150893</ref>

-	~~===Crystal structure~~ of ~~human STAM1 VHS domain in complex with ubiquitin===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_20150893}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3ldz]] is a 7 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Bos_taurus Bos taurus] and [http://en~~.~~wikipedia.org~~/~~wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LDZ OCA].~~	+

	==See Also==		==See Also==
	*[[Ubiquitin\|Ubiquitin]]		*[[Ubiquitin\|Ubiquitin]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:020150893<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]

OCA at 08:23, 20 October 2012

OCA — Sat, 20 Oct 2012 08:23:47 GMT

←Older revision		Revision as of 08:23, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3ldz]] is a 7 chain structure ~~of [[Ubiquitin]]~~ with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LDZ OCA].	+	[[3ldz]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LDZ OCA].

	==See Also==		==See Also==

OCA at 19:23, 25 July 2012

OCA — Wed, 25 Jul 2012 19:23:20 GMT

←Older revision		Revision as of 19:23, 25 July 2012
Line 1:		Line 1:
	[[Image:3ldz.png\|left\|200px]]		[[Image:3ldz.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3ldz", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3ldz\| PDB=3ldz \| SCENE= }}		{{STRUCTURE_3ldz\| PDB=3ldz \| SCENE= }}

	===Crystal structure of human STAM1 VHS domain in complex with ubiquitin===		===Crystal structure of human STAM1 VHS domain in complex with ubiquitin===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_20150893}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 20150893 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_20150893}}		{{ABSTRACT_PUBMED_20150893}}

Line 22:		Line 11:

	==See Also==		==See Also==
-	*[[Ubiquitin]]	+	*[[Ubiquitin\|Ubiquitin]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~20150893~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:020150893</ref><references group="xtra"/>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Hurley, J H.]]		[[Category: Hurley, J H.]]
	[[Category: Ren, X.]]		[[Category: Ren, X.]]
-	[[Category: Cytoplasm]]
	[[Category: Endosome]]		[[Category: Endosome]]
	[[Category: Membrane]]		[[Category: Membrane]]
Line 37:		Line 25:
	[[Category: Transport]]		[[Category: Transport]]
	[[Category: Ubiquitin-binding]]		[[Category: Ubiquitin-binding]]
-	[[Category: Ubl conjugation]]

OCA: Protected "3ldz" [edit=sysop:move=sysop]

OCA — Sun, 26 Dec 2010 23:02:35 GMT

Protected "3ldz" [edit=sysop:move=sysop]

←Older revision

Revision as of 23:02, 26 December 2010