3lkm - Revision history

OCA at 10:21, 21 February 2024

OCA — Wed, 21 Feb 2024 10:21:05 GMT

←Older revision		Revision as of 10:21, 21 February 2024
Line 1:		Line 1:

	==1.6 Angstrom Crystal Structure of the Alpha-kinase Domain of Myosin Heavy Chain Kinase A Complex with AMP==		==1.6 Angstrom Crystal Structure of the Alpha-kinase Domain of Myosin Heavy Chain Kinase A Complex with AMP==
-	<StructureSection load='3lkm' size='340' side='right' caption='[[3lkm]], [[Resolution\|resolution]] 1.60Å' scene=''>	+	<StructureSection load='3lkm' size='340' side='right'caption='[[3lkm]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3lkm]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Dicdi Dicdi~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LKM OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3LKM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3lkm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LKM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3LKM FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.6Å</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">DDB_G0291231, mhckA, mhkA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=44689 DICDI])</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/[Myosin_heavy-chain]_kinase [Myosin heavy-chain] kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.7 2.7.11.7] </span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3lkm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3lkm OCA], [https://pdbe.org/3lkm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3lkm RCSB], [https://www.ebi.ac.uk/pdbsum/3lkm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3lkm ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3lkm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3lkm OCA], [~~http~~://pdbe.org/3lkm PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3lkm RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3lkm PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3lkm ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/MHCKA_DICDI MHCKA_DICDI]] Phosphorylates threonine in the C-terminal tail region of myosin II heavy chain. This phosphorylation is critical in regulating the assembly and disassembly of myosin II filament. Requires autophosphorylation for activity.	+	[https://www.uniprot.org/uniprot/MHCKA_DICDI MHCKA_DICDI] Phosphorylates threonine in the C-terminal tail region of myosin II heavy chain. This phosphorylation is critical in regulating the assembly and disassembly of myosin II filament. Requires autophosphorylation for activity.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 21:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3lkm ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3lkm ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Dictyostelium discoideum myosin II heavy chain kinase A (MHCK A) disrupts the assembly and cellular activity of bipolar filaments of myosin II by phosphorylating sites within its alpha-helical, coiled-coil tail. MHCK A is a member of the atypical alpha-kinase family of serine and threonine protein kinases and displays no sequence homology to typical eukaryotic protein kinases. We report the crystal structure of the alpha-kinase domain (A-CAT) of MHCK A. When crystallized in the presence of adenosine triphosphate (ATP), A-CAT contained adenosine monophosphate (AMP) at the active site. However, when crystallized in the presence of ATP and a peptide substrate, which does not appear in the structure, adenosine diphosphate (ADP) was found at the active site and an invariant aspartic acid residue (Asp(766)) at the active site was phosphorylated. The aspartylphosphate group was exposed to the solvent within an active-site pocket that might function as a docking site for substrates. Access to the aspartylphosphate was regulated by a conformational switch in a loop that bound to a magnesium ion (Mg(2+)), providing a mechanism that allows alpha-kinases to sense and respond to local changes in Mg(2+).
-
-	Crystal structure of the alpha-kinase domain of Dictyostelium myosin heavy chain kinase A.,Ye Q, Crawley SW, Yang Y, Cote GP, Jia Z Sci Signal. 2010 Mar 2;3(111):ra17. PMID:20197546<ref>PMID:20197546</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3lkm" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Dicdi~~]]	+	[[Category: Dictyostelium discoideum]]
-	[[Category: ~~Jia, Z~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Ye, Q~~]]	+	[[Category: Jia Z]]
-	[[Category: ~~Alpha-kinase]]~~	+	[[Category: Ye Q]]
-	~~[[Category: Atp-binding]]~~	+
-	~~[[Category: Coiled coil]]~~	+
-	~~[[Category: Kinase]]~~	+
-	~~[[Category: Nucleotide-binding]]~~	+
-	~~[[Category: Protein kinase-like fold]]~~	+
-	~~[[Category: Serine/threonine-protein kinase]]~~	+
-	~~[[Category: Transferase]]~~	+
-	~~[[Category: Wd repeat~~]]	+

OCA at 06:43, 2 January 2019

OCA — Wed, 02 Jan 2019 06:43:09 GMT

←Older revision		Revision as of 06:43, 2 January 2019
Line 3:		Line 3:
	<StructureSection load='3lkm' size='340' side='right' caption='[[3lkm]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='3lkm' size='340' side='right' caption='[[3lkm]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3lkm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Atcc_11735 Atcc 11735~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LKM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3LKM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3lkm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dicdi Dicdi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LKM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3LKM FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">DDB_G0291231, mhckA, mhkA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=44689 ~~ATCC 11735~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">DDB_G0291231, mhckA, mhkA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=44689 DICDI])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/[Myosin_heavy-chain]_kinase [Myosin heavy-chain] kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.7 2.7.11.7] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/[Myosin_heavy-chain]_kinase [Myosin heavy-chain] kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.7 2.7.11.7] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3lkm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3lkm OCA], [http://pdbe.org/3lkm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3lkm RCSB], [http://www.ebi.ac.uk/pdbsum/3lkm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3lkm ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3lkm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3lkm OCA], [http://pdbe.org/3lkm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3lkm RCSB], [http://www.ebi.ac.uk/pdbsum/3lkm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3lkm ProSAT]</span></td></tr>
Line 15:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lk/3lkm_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lk/3lkm_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 34:		Line 34:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Atcc 11735~~]]	+	[[Category: Dicdi]]
	[[Category: Jia, Z]]		[[Category: Jia, Z]]
	[[Category: Ye, Q]]		[[Category: Ye, Q]]
	[[Category: Alpha-kinase]]		[[Category: Alpha-kinase]]
	[[Category: Atp-binding]]		[[Category: Atp-binding]]
		+	[[Category: Coiled coil]]
	[[Category: Kinase]]		[[Category: Kinase]]
	[[Category: Nucleotide-binding]]		[[Category: Nucleotide-binding]]

OCA at 18:10, 4 August 2016

OCA — Thu, 04 Aug 2016 18:10:26 GMT

←Older revision		Revision as of 18:10, 4 August 2016
Line 1:		Line 1:
		+
	==1.6 Angstrom Crystal Structure of the Alpha-kinase Domain of Myosin Heavy Chain Kinase A Complex with AMP==		==1.6 Angstrom Crystal Structure of the Alpha-kinase Domain of Myosin Heavy Chain Kinase A Complex with AMP==
	<StructureSection load='3lkm' size='340' side='right' caption='[[3lkm]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='3lkm' size='340' side='right' caption='[[3lkm]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3lkm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Dictyostelium_discoideum Dictyostelium discoideum~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LKM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3LKM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3lkm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_11735 Atcc 11735]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LKM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3LKM FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">DDB_G0291231, mhckA, mhkA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=44689 ~~Dictyostelium discoideum~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">DDB_G0291231, mhckA, mhkA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=44689 ATCC 11735])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/[Myosin_heavy-chain]_kinase [Myosin heavy-chain] kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.7 2.7.11.7] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/[Myosin_heavy-chain]_kinase [Myosin heavy-chain] kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.7 2.7.11.7] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3lkm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3lkm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3lkm RCSB], [http://www.ebi.ac.uk/pdbsum/3lkm PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3lkm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3lkm OCA], [http://pdbe.org/3lkm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3lkm RCSB], [http://www.ebi.ac.uk/pdbsum/3lkm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3lkm ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3lkm ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 28:		Line 29:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3lkm" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Dictyostelium discoideum~~]]	+	[[Category: Atcc 11735]]
	[[Category: Jia, Z]]		[[Category: Jia, Z]]
	[[Category: Ye, Q]]		[[Category: Ye, Q]]

OCA at 18:02, 24 December 2014

OCA — Wed, 24 Dec 2014 18:02:40 GMT

←Older revision		Revision as of 18:02, 24 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3lkm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3lkm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3lkm RCSB], [http://www.ebi.ac.uk/pdbsum/3lkm PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3lkm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3lkm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3lkm RCSB], [http://www.ebi.ac.uk/pdbsum/3lkm PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MHCKA_DICDI MHCKA_DICDI]] Phosphorylates threonine in the C-terminal tail region of myosin II heavy chain. This phosphorylation is critical in regulating the assembly and disassembly of myosin II filament. Requires autophosphorylation for activity.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 15:56, 18 December 2014

OCA — Thu, 18 Dec 2014 15:56:35 GMT

←Older revision		Revision as of 15:56, 18 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3lkm\| PDB=3lkm \| SCENE= }}~~	+	==1.6 Angstrom Crystal Structure of the Alpha-kinase Domain of Myosin Heavy Chain Kinase A Complex with AMP==
-	===1.6 Angstrom Crystal Structure of the Alpha-kinase Domain of Myosin Heavy Chain Kinase A Complex with AMP===	+	<StructureSection load='3lkm' size='340' side='right' caption='[[3lkm]], [[Resolution\|resolution]] 1.60Å' scene=''>
-	~~{{ABSTRACT_PUBMED_20197546}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3lkm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LKM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3LKM FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">DDB_G0291231, mhckA, mhkA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=44689 Dictyostelium discoideum])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/[Myosin_heavy-chain]_kinase [Myosin heavy-chain] kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.7 2.7.11.7] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3lkm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3lkm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3lkm RCSB], [http://www.ebi.ac.uk/pdbsum/3lkm PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lk/3lkm_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Dictyostelium discoideum myosin II heavy chain kinase A (MHCK A) disrupts the assembly and cellular activity of bipolar filaments of myosin II by phosphorylating sites within its alpha-helical, coiled-coil tail. MHCK A is a member of the atypical alpha-kinase family of serine and threonine protein kinases and displays no sequence homology to typical eukaryotic protein kinases. We report the crystal structure of the alpha-kinase domain (A-CAT) of MHCK A. When crystallized in the presence of adenosine triphosphate (ATP), A-CAT contained adenosine monophosphate (AMP) at the active site. However, when crystallized in the presence of ATP and a peptide substrate, which does not appear in the structure, adenosine diphosphate (ADP) was found at the active site and an invariant aspartic acid residue (Asp(766)) at the active site was phosphorylated. The aspartylphosphate group was exposed to the solvent within an active-site pocket that might function as a docking site for substrates. Access to the aspartylphosphate was regulated by a conformational switch in a loop that bound to a magnesium ion (Mg(2+)), providing a mechanism that allows alpha-kinases to sense and respond to local changes in Mg(2+).

-	~~==Function==~~	+	Crystal structure of the alpha-kinase domain of Dictyostelium myosin heavy chain kinase A.,Ye Q, Crawley SW, Yang Y, Cote GP, Jia Z Sci Signal. 2010 Mar 2;3(111):ra17. PMID:20197546<ref>PMID:20197546</ref>
-	~~[[http://www.uniprot.org/uniprot/MHCKA_DICDI MHCKA_DICDI]] Phosphorylates threonine in~~ the C-~~terminal tail region~~ of myosin II heavy chain. ~~This phosphorylation is critical in regulating the assembly and disassembly of myosin II filament~~. ~~Requires autophosphorylation for activity~~.	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[3lkm]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3LKM OCA].~~	+	</div>
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:020197546</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Dictyostelium discoideum]]		[[Category: Dictyostelium discoideum]]
-	[[Category: Jia, Z.]]	+	[[Category: Jia, Z]]
-	[[Category: Ye, Q.]]	+	[[Category: Ye, Q]]
	[[Category: Alpha-kinase]]		[[Category: Alpha-kinase]]
	[[Category: Atp-binding]]		[[Category: Atp-binding]]

OCA: Protected "3lkm" [edit=sysop:move=sysop]

OCA — Thu, 04 Apr 2013 20:20:03 GMT

Protected "3lkm" [edit=sysop:move=sysop]

←Older revision

Revision as of 20:20, 4 April 2013

OCA at 20:19, 4 April 2013

OCA — Thu, 04 Apr 2013 20:19:57 GMT

←Older revision		Revision as of 20:19, 4 April 2013
Line 1:		Line 1:
-	{{Seed}}
-	[[Image:3lkm.jpg\|left\|200px]]
-
-	<!--
-	The line below this paragraph, containing "STRUCTURE_3lkm", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3lkm\| PDB=3lkm \| SCENE= }}		{{STRUCTURE_3lkm\| PDB=3lkm \| SCENE= }}
-
	===1.6 Angstrom Crystal Structure of the Alpha-kinase Domain of Myosin Heavy Chain Kinase A Complex with AMP===		===1.6 Angstrom Crystal Structure of the Alpha-kinase Domain of Myosin Heavy Chain Kinase A Complex with AMP===
		+	{{ABSTRACT_PUBMED_20197546}}

-		+	==Function==
-	~~<!--~~	+	[[http://www.uniprot.org/uniprot/MHCKA_DICDI MHCKA_DICDI]] Phosphorylates threonine in the C-terminal tail region of myosin II heavy chain. This phosphorylation is critical in regulating the assembly and disassembly of myosin II filament. Requires autophosphorylation for activity.
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_20197546}}, adds the Publication Abstract to the page~~	+
-	~~(as it appears on PubMed at~~ http://www.~~pubmed~~.~~gov), where 20197546~~ is the ~~PubMed ID number~~.	+
-	~~-->~~	+
-	~~{{ABSTRACT_PUBMED_20197546}}~~	+

	==About this Structure==		==About this Structure==
-	~~3LKM~~ is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LKM OCA].	+	[[3lkm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LKM OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~20197546~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:020197546</ref><references group="xtra"/><references/>
	[[Category: Dictyostelium discoideum]]		[[Category: Dictyostelium discoideum]]
	[[Category: Jia, Z.]]		[[Category: Jia, Z.]]
Line 29:		Line 16:
	[[Category: Alpha-kinase]]		[[Category: Alpha-kinase]]
	[[Category: Atp-binding]]		[[Category: Atp-binding]]
-	[[Category: Coiled coil]]
	[[Category: Kinase]]		[[Category: Kinase]]
	[[Category: Nucleotide-binding]]		[[Category: Nucleotide-binding]]
Line 36:		Line 22:
	[[Category: Transferase]]		[[Category: Transferase]]
	[[Category: Wd repeat]]		[[Category: Wd repeat]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 17 09:34:52 2010''

OCA at 07:34, 17 March 2010

OCA — Wed, 17 Mar 2010 07:34:53 GMT

←Older revision		Revision as of 07:34, 17 March 2010
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3lkm.jpg\|left\|200px]]

-	The ~~entry 3lkm~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3lkm", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3lkm\| PDB=3lkm \| SCENE= }}

-	~~Authors: Ye, Q., Jia, Z~~.	+	===1.6 Angstrom Crystal Structure of the Alpha-kinase Domain of Myosin Heavy Chain Kinase A Complex with AMP===

-	Description: 1.6 Angstrom Crystal Structure of the Alpha-kinase Domain of Myosin Heavy Chain Kinase A Complex with AMP

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Feb 10 16~~:58:58 2010''	+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_20197546}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 20197546 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_20197546}}
		+
		+	==About this Structure==
		+	3LKM is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LKM OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:20197546</ref><references group="xtra"/>
		+	[[Category: Dictyostelium discoideum]]
		+	[[Category: Jia, Z.]]
		+	[[Category: Ye, Q.]]
		+	[[Category: Alpha-kinase]]
		+	[[Category: Atp-binding]]
		+	[[Category: Coiled coil]]
		+	[[Category: Kinase]]
		+	[[Category: Nucleotide-binding]]
		+	[[Category: Protein kinase-like fold]]
		+	[[Category: Serine/threonine-protein kinase]]
		+	[[Category: Transferase]]
		+	[[Category: Wd repeat]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 17 09:34:52 2010''

OCA at 14:58, 10 February 2010

OCA — Wed, 10 Feb 2010 14:58:58 GMT

←Older revision		Revision as of 14:58, 10 February 2010
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3lkm is ON HOLD	+	The entry 3lkm is ON HOLD until Paper Publication

-	Authors: Ye, ~~Qilu~~, Jia, ~~Zongchao~~	+	Authors: Ye, Q., Jia, Z.

-	Description: Crystal Structure of the Alpha-kinase Domain of ~~Dictyostelium~~ Myosin Heavy Chain Kinase	+	Description: 1.6 Angstrom Crystal Structure of the Alpha-kinase Domain of Myosin Heavy Chain Kinase A Complex with AMP

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb ~~3 09~~:20:46 2010''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 10 16:58:58 2010''

OCA: New page: '''Unreleased structure''' The entry 3lkm is ON HOLD Authors: Ye, Qilu, Jia, Zongchao Description: Crystal Structure of the Alpha-kinase Domain of Dictyostelium Myosin Heavy Chain Kina...

OCA — Wed, 03 Feb 2010 07:20:47 GMT

New page: '''Unreleased structure''' The entry 3lkm is ON HOLD Authors: Ye, Qilu, Jia, Zongchao Description: Crystal Structure of the Alpha-kinase Domain of Dictyostelium Myosin Heavy Chain Kina...

New page

'''Unreleased structure'''

The entry 3lkm is ON HOLD

Authors: Ye, Qilu, Jia, Zongchao

Description: Crystal Structure of the Alpha-kinase Domain of Dictyostelium Myosin Heavy Chain Kinase

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 3 09:20:46 2010''