3ltq - Revision history

OCA at 08:47, 7 February 2024

OCA — Wed, 07 Feb 2024 08:47:02 GMT

←Older revision		Revision as of 08:47, 7 February 2024
Line 3:		Line 3:
	<StructureSection load='3ltq' size='340' side='right'caption='[[3ltq]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='3ltq' size='340' side='right'caption='[[3ltq]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3ltq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LTQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3LTQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3ltq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LTQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3LTQ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TB:TERBIUM(III)+ION'>TB</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IL1B, IL1F2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TB:TERBIUM(III)+ION'>TB</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ltq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ltq OCA], [https://pdbe.org/3ltq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ltq RCSB], [https://www.ebi.ac.uk/pdbsum/3ltq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ltq ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ltq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ltq OCA], [https://pdbe.org/3ltq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ltq RCSB], [https://www.ebi.ac.uk/pdbsum/3ltq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ltq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/IL1B_HUMAN IL1B_HUMAN]] Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.<ref>PMID:3920526</ref>	+	[https://www.uniprot.org/uniprot/IL1B_HUMAN IL1B_HUMAN] Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.<ref>PMID:3920526</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Lanthanide-binding tags (LBTs) are valuable tools for investigation of protein structure, function, and dynamics by NMR spectroscopy, X-ray crystallography, and luminescence studies. We have inserted LBTs into three different loop positions (denoted L, R, and S) of the model protein interleukin-1beta (IL1beta) and varied the length of the spacer between the LBT and the protein (denoted 1-3). Luminescence studies demonstrate that all nine constructs bind Tb(3+) tightly in the low nanomolar range. No significant change in the fusion protein occurs from insertion of the LBT, as shown by two X-ray crystallographic structures of the IL1beta-S1 and IL1beta-L3 constructs and for the remaining constructs by comparing the (1)H-(15)N heteronuclear single-quantum coherence NMR spectra with that of the wild-type IL1beta. Additionally, binding of LBT-loop IL1beta proteins to their native binding partner in vitro remains unaltered. X-ray crystallographic phasing was successful using only the signal from the bound lanthanide. Large residual dipolar couplings (RDCs) could be determined by NMR spectroscopy for all LBT-loop constructs and revealed that the LBT-2 series were rigidly incorporated into the interleukin-1beta structure. The paramagnetic NMR spectra of loop-LBT mutant IL1beta-R2 were assigned and the Deltachi tensor components were calculated on the basis of RDCs and pseudocontact shifts. A structural model of the IL1beta-R2 construct was calculated using the paramagnetic restraints. The current data provide support that encodable LBTs serve as versatile biophysical tags when inserted into loop regions of proteins of known structure or predicted via homology modeling.	+
-		+
-	Engineering Encodable Lanthanide-Binding Tags into Loop Regions of Proteins.,Barthelmes K, Reynolds AM, Peisach E, Jonker HR, Denunzio NJ, Allen KN, Imperiali B, Schwalbe H J Am Chem Soc. 2010 Dec 23. PMID:21182275<ref>PMID:21182275</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3ltq" style="background-color:#fffaf0;"></div~~>	+

	==See Also==		==See Also==
Line 26:		Line 17:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Allen~~, K N~~]]	+	[[Category: Allen KN]]
-	[[Category: Peisach, E]]	+	[[Category: Peisach E]]
-	~~[[Category: Cytokine]]~~	+
-	~~[[Category: Il1b]]~~	+
-	~~[[Category: Inflammatory response]]~~	+
-	~~[[Category: Lanthanide binding tag]]~~	+
-	~~[[Category: Lbt]]~~	+
-	~~[[Category: Mitogen]]~~	+
-	~~[[Category: Pyrogen]]~~	+
-	~~[[Category: Secreted]]~~	+
-	~~[[Category: Terbium~~]]	+

OCA at 13:42, 4 May 2022

OCA — Wed, 04 May 2022 13:42:39 GMT

←Older revision		Revision as of 13:42, 4 May 2022
Line 1:		Line 1:

	==Structure of Interleukin 1B solved by SAD using an inserted Lanthanide Binding Tag==		==Structure of Interleukin 1B solved by SAD using an inserted Lanthanide Binding Tag==
-	<StructureSection load='3ltq' size='340' side='right' caption='[[3ltq]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='3ltq' size='340' side='right'caption='[[3ltq]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3ltq]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LTQ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3LTQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3ltq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LTQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3LTQ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TB:TERBIUM(III)+ION'>TB</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TB:TERBIUM(III)+ION'>TB</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IL1B, IL1F2 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IL1B, IL1F2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3ltq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ltq OCA], [~~http~~://pdbe.org/3ltq PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3ltq RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3ltq PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3ltq ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ltq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ltq OCA], [https://pdbe.org/3ltq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ltq RCSB], [https://www.ebi.ac.uk/pdbsum/3ltq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ltq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/IL1B_HUMAN IL1B_HUMAN]] Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.<ref>PMID:3920526</ref>	+	[[https://www.uniprot.org/uniprot/IL1B_HUMAN IL1B_HUMAN]] Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.<ref>PMID:3920526</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 21:

	==See Also==		==See Also==
-	*[[Interleukin\|Interleukin]]	+	*[[Interleukin 3D structures\|Interleukin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 27:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Allen, K N]]		[[Category: Allen, K N]]
	[[Category: Peisach, E]]		[[Category: Peisach, E]]

OCA at 14:12, 5 August 2016

OCA — Fri, 05 Aug 2016 14:12:19 GMT

←Older revision		Revision as of 14:12, 5 August 2016
Line 1:		Line 1:
		+
	==Structure of Interleukin 1B solved by SAD using an inserted Lanthanide Binding Tag==		==Structure of Interleukin 1B solved by SAD using an inserted Lanthanide Binding Tag==
	<StructureSection load='3ltq' size='340' side='right' caption='[[3ltq]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='3ltq' size='340' side='right' caption='[[3ltq]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3ltq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LTQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3LTQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3ltq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LTQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3LTQ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TB:TERBIUM(III)+ION'>TB</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TB:TERBIUM(III)+ION'>TB</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IL1B, IL1F2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IL1B, IL1F2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ltq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ltq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ltq RCSB], [http://www.ebi.ac.uk/pdbsum/3ltq PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ltq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ltq OCA], [http://pdbe.org/3ltq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ltq RCSB], [http://www.ebi.ac.uk/pdbsum/3ltq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ltq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3ltq" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 24:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Allen, K N]]		[[Category: Allen, K N]]
	[[Category: Peisach, E]]		[[Category: Peisach, E]]

OCA at 03:09, 25 December 2014

OCA — Thu, 25 Dec 2014 03:09:37 GMT

←Older revision		Revision as of 03:09, 25 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ltq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ltq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ltq RCSB], [http://www.ebi.ac.uk/pdbsum/3ltq PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ltq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ltq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ltq RCSB], [http://www.ebi.ac.uk/pdbsum/3ltq PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/IL1B_HUMAN IL1B_HUMAN]] Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.<ref>PMID:3920526</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 10:05, 9 December 2014

OCA — Tue, 09 Dec 2014 10:05:14 GMT

←Older revision		Revision as of 10:05, 9 December 2014
Line 1:		Line 1:
-	[[~~Image:~~3ltq.~~png~~\|~~left~~\|~~200px~~]]	+	==Structure of Interleukin 1B solved by SAD using an inserted Lanthanide Binding Tag==
		+	<StructureSection load='3ltq' size='340' side='right' caption='[[3ltq]], [[Resolution\|resolution]] 2.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3ltq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LTQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3LTQ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TB:TERBIUM(III)+ION'>TB</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IL1B, IL1F2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ltq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ltq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ltq RCSB], [http://www.ebi.ac.uk/pdbsum/3ltq PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Lanthanide-binding tags (LBTs) are valuable tools for investigation of protein structure, function, and dynamics by NMR spectroscopy, X-ray crystallography, and luminescence studies. We have inserted LBTs into three different loop positions (denoted L, R, and S) of the model protein interleukin-1beta (IL1beta) and varied the length of the spacer between the LBT and the protein (denoted 1-3). Luminescence studies demonstrate that all nine constructs bind Tb(3+) tightly in the low nanomolar range. No significant change in the fusion protein occurs from insertion of the LBT, as shown by two X-ray crystallographic structures of the IL1beta-S1 and IL1beta-L3 constructs and for the remaining constructs by comparing the (1)H-(15)N heteronuclear single-quantum coherence NMR spectra with that of the wild-type IL1beta. Additionally, binding of LBT-loop IL1beta proteins to their native binding partner in vitro remains unaltered. X-ray crystallographic phasing was successful using only the signal from the bound lanthanide. Large residual dipolar couplings (RDCs) could be determined by NMR spectroscopy for all LBT-loop constructs and revealed that the LBT-2 series were rigidly incorporated into the interleukin-1beta structure. The paramagnetic NMR spectra of loop-LBT mutant IL1beta-R2 were assigned and the Deltachi tensor components were calculated on the basis of RDCs and pseudocontact shifts. A structural model of the IL1beta-R2 construct was calculated using the paramagnetic restraints. The current data provide support that encodable LBTs serve as versatile biophysical tags when inserted into loop regions of proteins of known structure or predicted via homology modeling.

-	~~{{STRUCTURE_3ltq\| PDB=3ltq \| SCENE= }}~~	+	Engineering Encodable Lanthanide-Binding Tags into Loop Regions of Proteins.,Barthelmes K, Reynolds AM, Peisach E, Jonker HR, Denunzio NJ, Allen KN, Imperiali B, Schwalbe H J Am Chem Soc. 2010 Dec 23. PMID:21182275<ref>PMID:21182275</ref>

-	~~===Structure~~ of ~~Interleukin 1B solved by SAD using an inserted Lanthanide Binding Tag===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_21182275}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3ltq]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3LTQ OCA].~~	+

	==See Also==		==See Also==
	*[[Interleukin\|Interleukin]]		*[[Interleukin\|Interleukin]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:021182275<~~/~~ref~~><~~ref group="xtra">PMID:017497863</ref><references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Allen, K N.]]	+	[[Category: Allen, K N]]
-	[[Category: Peisach, E.]]	+	[[Category: Peisach, E]]
	[[Category: Cytokine]]		[[Category: Cytokine]]
	[[Category: Il1b]]		[[Category: Il1b]]

OCA at 11:58, 20 October 2012

OCA — Sat, 20 Oct 2012 11:58:42 GMT

←Older revision		Revision as of 11:58, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3ltq]] is a 1 chain structure ~~of [[Interleukin]]~~ with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LTQ OCA].	+	[[3ltq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LTQ OCA].

	==See Also==		==See Also==

OCA at 07:13, 26 July 2012

OCA — Thu, 26 Jul 2012 07:13:18 GMT

←Older revision		Revision as of 07:13, 26 July 2012
Line 1:		Line 1:
	[[Image:3ltq.png\|left\|200px]]		[[Image:3ltq.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3ltq", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3ltq\| PDB=3ltq \| SCENE= }}		{{STRUCTURE_3ltq\| PDB=3ltq \| SCENE= }}

	===Structure of Interleukin 1B solved by SAD using an inserted Lanthanide Binding Tag===		===Structure of Interleukin 1B solved by SAD using an inserted Lanthanide Binding Tag===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_21182275}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 21182275 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_21182275}}		{{ABSTRACT_PUBMED_21182275}}

	==About this Structure==		==About this Structure==
-	[[3ltq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LTQ OCA].	+	[[3ltq]] is a 1 chain structure of [[Interleukin]] with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LTQ OCA].
		+
		+	==See Also==
		+	*[[Interleukin\|Interleukin]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~21182275~~</ref><ref group="xtra">PMID:~~17497863~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:021182275</ref><ref group="xtra">PMID:017497863</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Allen, K N.]]		[[Category: Allen, K N.]]
	[[Category: Peisach, E.]]		[[Category: Peisach, E.]]
		+	[[Category: Cytokine]]
		+	[[Category: Il1b]]
		+	[[Category: Inflammatory response]]
		+	[[Category: Lanthanide binding tag]]
		+	[[Category: Lbt]]
		+	[[Category: Mitogen]]
		+	[[Category: Pyrogen]]
		+	[[Category: Secreted]]
		+	[[Category: Terbium]]

OCA at 19:26, 14 March 2011

OCA — Mon, 14 Mar 2011 19:26:46 GMT

←Older revision		Revision as of 19:26, 14 March 2011
Line 1:		Line 1:
-	[[Image:3ltq.~~jpg~~\|left\|200px]]	+	[[Image:3ltq.png\|left\|200px]]

	<!--		<!--

OCA: Protected "3ltq" [edit=sysop:move=sysop]

OCA — Wed, 16 Feb 2011 05:38:26 GMT

Protected "3ltq" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:38, 16 February 2011

OCA at 05:38, 16 February 2011

OCA — Wed, 16 Feb 2011 05:38:23 GMT

←Older revision		Revision as of 05:38, 16 February 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3ltq.jpg\|left\|200px]]

-	The ~~entry 3ltq~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3ltq", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3ltq\| PDB=3ltq \| SCENE= }}

-	~~Authors: Peisach, E., Allen, K.N.~~	+	===Structure of Interleukin 1B solved by SAD using an inserted Lanthanide Binding Tag===

-	Description: Structure of Interleukin 1B solved by SAD using an inserted Lanthanide Binding Tag

-	~~''Page seeded by~~ [http://oca.weizmann.ac.il/oca OCA ] ~~on Wed Aug 11 23~~:32:~~43 2010''~~	+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21182275}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21182275 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21182275}}
		+
		+	==About this Structure==
		+	[[3ltq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LTQ OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:21182275</ref><ref group="xtra">PMID:17497863</ref><references group="xtra"/>
		+	[[Category: Homo sapiens]]
		+	[[Category: Allen, K N.]]
		+	[[Category: Peisach, E.]]