3lzd - Revision history

OCA at 08:46, 6 September 2023

OCA — Wed, 06 Sep 2023 08:46:19 GMT

←Older revision		Revision as of 08:46, 6 September 2023
Line 3:		Line 3:
	<StructureSection load='3lzd' size='340' side='right'caption='[[3lzd]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='3lzd' size='340' side='right'caption='[[3lzd]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3lzd]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~'pyrococcus_shinkaii' 'pyrococcus shinkaii'~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LZD OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=3LZD FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3lzd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LZD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3LZD FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>~~, ~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~[[3lzc\|3lzc]]~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=3lzd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3lzd OCA], [~~http~~://pdbe.org/3lzd PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3lzd RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3lzd PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3lzd ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3lzd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3lzd OCA], [https://pdbe.org/3lzd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3lzd RCSB], [https://www.ebi.ac.uk/pdbsum/3lzd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3lzd ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/DPH2_PYRHO DPH2_PYRHO]] Catalyzes the first step of diphthamide biosynthesis, i.e. the transfer of the 3-amino-3-carboxypropyl group from S-adenosyl-L-methionine (SAM) to the C2 position of the imidazole ring of the target histidine residue in translation elongation factor 2 (EF-2).<ref>PMID:20559380</ref>	+	[https://www.uniprot.org/uniprot/DPH2_PYRHO DPH2_PYRHO] Catalyzes the first step of diphthamide biosynthesis, i.e. the transfer of the 3-amino-3-carboxypropyl group from S-adenosyl-L-methionine (SAM) to the C2 position of the imidazole ring of the target histidine residue in translation elongation factor 2 (EF-2).<ref>PMID:20559380</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Pyrococcus shinkaii]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Dzikovski, B]]	+	[[Category: Pyrococcus horikoshii]]
-	[[Category: Ealick~~, S E~~]]	+	[[Category: Dzikovski B]]
-	[[Category: Freed, J]]	+	[[Category: Ealick SE]]
-	[[Category: Koralewski~~, R M~~]]	+	[[Category: Freed J]]
-	[[Category: Krebs, C]]	+	[[Category: Koralewski RM]]
-	[[Category: Lee, M]]	+	[[Category: Krebs C]]
-	[[Category: Lin, H]]	+	[[Category: Lee M]]
-	[[Category: Torelli~~, A T~~]]	+	[[Category: Lin H]]
-	[[Category: Wang, E]]	+	[[Category: Torelli AT]]
-	[[Category: Zhang, Y]]	+	[[Category: Wang E]]
-	[[Category: Zhu, X]]	+	[[Category: Zhang Y]]
-	~~[[Category: Biosynthetic protein]]~~	+	[[Category: Zhu X]]
-	~~[[Category: Diphthamide biosynthesis]]~~	+
-	~~[[Category: Gene triplication]]~~	+
-	~~[[Category: Iron-sulfur cluster]]~~	+
-	~~[[Category: Radical sam enzyme~~]]	+

OCA at 06:38, 14 October 2020

OCA — Wed, 14 Oct 2020 06:38:39 GMT

←Older revision		Revision as of 06:38, 14 October 2020
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	==Crystal structure of Dph2 from Pyrococcus horikoshii with 4Fe-4S cluster==		==Crystal structure of Dph2 from Pyrococcus horikoshii with 4Fe-4S cluster==
-	<StructureSection load='3lzd' size='340' side='right' caption='[[3lzd]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='3lzd' size='340' side='right'caption='[[3lzd]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3lzd]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/'pyrococcus_shinkaii' 'pyrococcus shinkaii']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LZD OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3LZD FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3lzd]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/'pyrococcus_shinkaii' 'pyrococcus shinkaii']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LZD OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3LZD FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3lzc\|3lzc]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3lzc\|3lzc]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3lzd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3lzd OCA], [http://pdbe.org/3lzd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3lzd RCSB], [http://www.ebi.ac.uk/pdbsum/3lzd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3lzd ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3lzd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3lzd OCA], [http://pdbe.org/3lzd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3lzd RCSB], [http://www.ebi.ac.uk/pdbsum/3lzd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3lzd ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 34:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Pyrococcus shinkaii]]		[[Category: Pyrococcus shinkaii]]
		+	[[Category: Large Structures]]
	[[Category: Dzikovski, B]]		[[Category: Dzikovski, B]]
	[[Category: Ealick, S E]]		[[Category: Ealick, S E]]

OCA at 07:19, 9 January 2019

OCA — Wed, 09 Jan 2019 07:19:03 GMT

←Older revision		Revision as of 07:19, 9 January 2019
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lz/3lzd_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lz/3lzd_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 10:12, 4 August 2016

OCA — Thu, 04 Aug 2016 10:12:21 GMT

←Older revision		Revision as of 10:12, 4 August 2016
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		+
	==Crystal structure of Dph2 from Pyrococcus horikoshii with 4Fe-4S cluster==		==Crystal structure of Dph2 from Pyrococcus horikoshii with 4Fe-4S cluster==
	<StructureSection load='3lzd' size='340' side='right' caption='[[3lzd]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='3lzd' size='340' side='right' caption='[[3lzd]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3lzd]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Pyrococcus_horikoshii Pyrococcus horikoshii~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LZD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3LZD FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3lzd]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/'pyrococcus_shinkaii' 'pyrococcus shinkaii']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LZD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3LZD FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3lzc\|3lzc]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3lzc\|3lzc]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3lzd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3lzd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3lzd RCSB], [http://www.ebi.ac.uk/pdbsum/3lzd PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3lzd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3lzd OCA], [http://pdbe.org/3lzd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3lzd RCSB], [http://www.ebi.ac.uk/pdbsum/3lzd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3lzd ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/~~O58832_PYRHO O58832_PYRHO~~]] Catalyzes the first step of diphthamide biosynthesis, i.e. the transfer of the 3-amino-3-carboxypropyl group from S-adenosyl-L-methionine (SAM) to the C2 position of the imidazole ring of the target histidine residue in translation elongation factor 2 (EF-2).<ref>PMID:20559380</ref>	+	[[http://www.uniprot.org/uniprot/DPH2_PYRHO DPH2_PYRHO]] Catalyzes the first step of diphthamide biosynthesis, i.e. the transfer of the 3-amino-3-carboxypropyl group from S-adenosyl-L-methionine (SAM) to the C2 position of the imidazole ring of the target histidine residue in translation elongation factor 2 (EF-2).<ref>PMID:20559380</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3lzd ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3lzd" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Pyrococcus ~~horikoshii~~]]	+	[[Category: Pyrococcus shinkaii]]
	[[Category: Dzikovski, B]]		[[Category: Dzikovski, B]]
	[[Category: Ealick, S E]]		[[Category: Ealick, S E]]

OCA at 08:46, 24 December 2014

OCA — Wed, 24 Dec 2014 08:46:07 GMT

←Older revision		Revision as of 08:46, 24 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3lzd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3lzd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3lzd RCSB], [http://www.ebi.ac.uk/pdbsum/3lzd PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3lzd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3lzd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3lzd RCSB], [http://www.ebi.ac.uk/pdbsum/3lzd PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/O58832_PYRHO O58832_PYRHO]] Catalyzes the first step of diphthamide biosynthesis, i.e. the transfer of the 3-amino-3-carboxypropyl group from S-adenosyl-L-methionine (SAM) to the C2 position of the imidazole ring of the target histidine residue in translation elongation factor 2 (EF-2).<ref>PMID:20559380</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 16:57, 18 December 2014

OCA — Thu, 18 Dec 2014 16:57:07 GMT

←Older revision		Revision as of 16:57, 18 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3lzd\| PDB=3lzd \| SCENE= }}~~	+	==Crystal structure of Dph2 from Pyrococcus horikoshii with 4Fe-4S cluster==
-	===Crystal structure of Dph2 from Pyrococcus horikoshii with 4Fe-4S cluster===	+	<StructureSection load='3lzd' size='340' side='right' caption='[[3lzd]], [[Resolution\|resolution]] 2.10Å' scene=''>
-	~~{{ABSTRACT_PUBMED_20559380}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3lzd]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LZD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3LZD FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3lzc\|3lzc]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3lzd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3lzd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3lzd RCSB], [http://www.ebi.ac.uk/pdbsum/3lzd PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lz/3lzd_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Archaeal and eukaryotic translation elongation factor 2 contain a unique post-translationally modified histidine residue called diphthamide, which is the target of diphtheria toxin. The biosynthesis of diphthamide was proposed to involve three steps, with the first being the formation of a C-C bond between the histidine residue and the 3-amino-3-carboxypropyl group of S-adenosyl-l-methionine (SAM). However, further details of the biosynthesis remain unknown. Here we present structural and biochemical evidence showing that the first step of diphthamide biosynthesis in the archaeon Pyrococcus horikoshii uses a novel iron-sulphur-cluster enzyme, Dph2. Dph2 is a homodimer and each of its monomers can bind a [4Fe-4S] cluster. Biochemical data suggest that unlike the enzymes in the radical SAM superfamily, Dph2 does not form the canonical 5'-deoxyadenosyl radical. Instead, it breaks the C(gamma,Met)-S bond of SAM and generates a 3-amino-3-carboxypropyl radical. Our results suggest that P. horikoshii Dph2 represents a previously unknown, SAM-dependent, [4Fe-4S]-containing enzyme that catalyses unprecedented chemistry.

-	~~==Function==~~	+	Diphthamide biosynthesis requires an organic radical generated by an iron-sulphur enzyme.,Zhang Y, Zhu X, Torelli AT, Lee M, Dzikovski B, Koralewski RM, Wang E, Freed J, Krebs C, Ealick SE, Lin H Nature. 2010 Jun 17;465(7300):891-6. PMID:20559380<ref>PMID:20559380</ref>
-	~~[[http://www.uniprot.org/uniprot/O58832_PYRHO O58832_PYRHO]] Catalyzes the first step of diphthamide~~ biosynthesis, i.~~e. the transfer of the 3-amino-3-carboxypropyl group from S-adenosyl-L-methionine~~ (~~SAM~~) ~~to the C2 position of the imidazole ring of the target histidine residue in translation elongation factor 2 (EF~~-2).<ref>PMID:20559380</ref>	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[3lzd]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3LZD OCA].~~	+	</div>
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:020559380</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Pyrococcus horikoshii]]		[[Category: Pyrococcus horikoshii]]
-	[[Category: Dzikovski, B.]]	+	[[Category: Dzikovski, B]]
-	[[Category: Ealick, S E.]]	+	[[Category: Ealick, S E]]
-	[[Category: Freed, J.]]	+	[[Category: Freed, J]]
-	[[Category: Koralewski, R M.]]	+	[[Category: Koralewski, R M]]
-	[[Category: Krebs, C.]]	+	[[Category: Krebs, C]]
-	[[Category: Lee, M.]]	+	[[Category: Lee, M]]
-	[[Category: Lin, H.]]	+	[[Category: Lin, H]]
-	[[Category: Torelli, A T.]]	+	[[Category: Torelli, A T]]
-	[[Category: Wang, E.]]	+	[[Category: Wang, E]]
-	[[Category: Zhang, Y.]]	+	[[Category: Zhang, Y]]
-	[[Category: Zhu, X.]]	+	[[Category: Zhu, X]]
	[[Category: Biosynthetic protein]]		[[Category: Biosynthetic protein]]
	[[Category: Diphthamide biosynthesis]]		[[Category: Diphthamide biosynthesis]]

OCA: Protected "3lzd" [edit=sysop:move=sysop]

OCA — Wed, 10 Apr 2013 23:32:32 GMT

Protected "3lzd" [edit=sysop:move=sysop]

←Older revision

Revision as of 23:32, 10 April 2013

OCA at 23:32, 10 April 2013

OCA — Wed, 10 Apr 2013 23:32:29 GMT

←Older revision		Revision as of 23:32, 10 April 2013
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-	{{Seed}}
-	[[Image:3lzd.jpg\|left\|200px]]
-
-	<!--
-	The line below this paragraph, containing "STRUCTURE_3lzd", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3lzd\| PDB=3lzd \| SCENE= }}		{{STRUCTURE_3lzd\| PDB=3lzd \| SCENE= }}
-
	===Crystal structure of Dph2 from Pyrococcus horikoshii with 4Fe-4S cluster===		===Crystal structure of Dph2 from Pyrococcus horikoshii with 4Fe-4S cluster===
		+	{{ABSTRACT_PUBMED_20559380}}

-		+	==Function==
-	~~<!--~~	+	[[http://www.uniprot.org/uniprot/O58832_PYRHO O58832_PYRHO]] Catalyzes the first step of diphthamide biosynthesis, i.e. the transfer of the 3-amino-3-carboxypropyl group from S-adenosyl-L-methionine (SAM) to the C2 position of the imidazole ring of the target histidine residue in translation elongation factor 2 (EF-2).<ref>PMID:20559380</ref>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_20559380}}, adds the Publication Abstract to the page~~	+
-	~~(as it appears on PubMed at~~ http://www.~~pubmed~~.~~gov), where 20559380 is~~ the ~~PubMed ID number~~.	+
-	-->	+
-	~~{{ABSTRACT_PUBMED_20559380}}~~	+

	==About this Structure==		==About this Structure==
-	~~3LZD~~ is a 2 ~~chains~~ structure with ~~sequences~~ from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LZD OCA].	+	[[3lzd]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LZD OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~20559380~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:020559380</ref><references group="xtra"/><references/>
	[[Category: Pyrococcus horikoshii]]		[[Category: Pyrococcus horikoshii]]
	[[Category: Dzikovski, B.]]		[[Category: Dzikovski, B.]]
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	[[Category: Iron-sulfur cluster]]		[[Category: Iron-sulfur cluster]]
	[[Category: Radical sam enzyme]]		[[Category: Radical sam enzyme]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 14 16:20:25 2010''

OCA at 12:14, 14 July 2010

OCA — Wed, 14 Jul 2010 12:14:17 GMT

←Older revision		Revision as of 12:14, 14 July 2010
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3lzd.jpg\|left\|200px]]

-	The ~~entry 3lzd~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3lzd", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3lzd\| PDB=3lzd \| SCENE= }}

-	~~Authors: Torelli, A.T., Zhang, Y., Zhu, X., Lee, M., Dzikovski, B., Koralewski, R.M., Wang, E., Freed, J., Krebs, C., Lin, H., Ealick, S.E.~~	+	===Crystal structure of Dph2 from Pyrococcus horikoshii with 4Fe-4S cluster===

-	Description: Crystal structure of Dph2 from Pyrococcus horikoshii with 4Fe-4S cluster

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Apr 21 09~~:34:41 2010''	+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_20559380}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 20559380 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_20559380}}
		+
		+	==About this Structure==
		+	3LZD is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LZD OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:20559380</ref><references group="xtra"/>
		+	[[Category: Pyrococcus horikoshii]]
		+	[[Category: Dzikovski, B.]]
		+	[[Category: Ealick, S E.]]
		+	[[Category: Freed, J.]]
		+	[[Category: Koralewski, R M.]]
		+	[[Category: Krebs, C.]]
		+	[[Category: Lee, M.]]
		+	[[Category: Lin, H.]]
		+	[[Category: Torelli, A T.]]
		+	[[Category: Wang, E.]]
		+	[[Category: Zhang, Y.]]
		+	[[Category: Zhu, X.]]
		+	[[Category: Biosynthetic protein]]
		+	[[Category: Diphthamide biosynthesis]]
		+	[[Category: Gene triplication]]
		+	[[Category: Iron-sulfur cluster]]
		+	[[Category: Radical sam enzyme]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 14 16:20:25 2010''

OCA at 06:34, 21 April 2010

OCA — Wed, 21 Apr 2010 06:34:42 GMT

←Older revision		Revision as of 06:34, 21 April 2010
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3lzd is ON HOLD	+	The entry 3lzd is ON HOLD until Paper Publication

	Authors: Torelli, A.T., Zhang, Y., Zhu, X., Lee, M., Dzikovski, B., Koralewski, R.M., Wang, E., Freed, J., Krebs, C., Lin, H., Ealick, S.E.		Authors: Torelli, A.T., Zhang, Y., Zhu, X., Lee, M., Dzikovski, B., Koralewski, R.M., Wang, E., Freed, J., Krebs, C., Lin, H., Ealick, S.E.
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	Description: Crystal structure of Dph2 from Pyrococcus horikoshii with 4Fe-4S cluster		Description: Crystal structure of Dph2 from Pyrococcus horikoshii with 4Fe-4S cluster

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Mar 10 13~~:17:20 2010''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 21 09:34:41 2010''