3m0u - Revision history

OCA at 06:36, 19 July 2023

OCA — Wed, 19 Jul 2023 06:36:29 GMT

←Older revision		Revision as of 06:36, 19 July 2023
Line 3:		Line 3:
	<StructureSection load='3m0u' size='340' side='right'caption='[[3m0u]], [[Resolution\|resolution]] 1.10Å' scene=''>		<StructureSection load='3m0u' size='340' side='right'caption='[[3m0u]], [[Resolution\|resolution]] 1.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3m0u]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Chick Chick~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M0U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3M0U FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3m0u]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M0U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3M0U FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.1Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'~~>~~[[1shg\|1shg]], [[3i9q\|3i9q]], [[2f2w\|2f2w]], [[2f2x\|2f2x]], [[3m0p\|3m0p]], [[3m0q\|3m0q]], [[3m0r\|3m0r]], [[3m0s\|3m0s]], [[3m0t\|3m0t]]~~<~~/div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">SPTAN1, SPTA2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 CHICK])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3m0u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m0u OCA], [https://pdbe.org/3m0u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3m0u RCSB], [https://www.ebi.ac.uk/pdbsum/3m0u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3m0u ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3m0u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m0u OCA], [https://pdbe.org/3m0u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3m0u RCSB], [https://www.ebi.ac.uk/pdbsum/3m0u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3m0u ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/SPTN1_CHICK SPTN1_CHICK]] Morphologically, spectrin-like proteins appear to be related to spectrin, showing a flexible rod-like structure. They can bind actin but seem to differ in their calmodulin-binding activity. In nonerythroid tissues, spectrins, in association with some other proteins, may play an important role in membrane organization.	+	[https://www.uniprot.org/uniprot/SPTN1_CHICK SPTN1_CHICK] Morphologically, spectrin-like proteins appear to be related to spectrin, showing a flexible rod-like structure. They can bind actin but seem to differ in their calmodulin-binding activity. In nonerythroid tissues, spectrins, in association with some other proteins, may play an important role in membrane organization.
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	alpha-Spectrin SH3-domain (Spc-SH3) crystallization is characterized by very fast growth of the crystals in the presence of ammonium sulfate as a precipitant agent. The origin of this behaviour can be attributed to the presence of a proline residue that participates in a crystal contact mimicking the binding of proline-rich sequences to SH3 domains. This residue, Pro20, is located in the RT loop and is the main contact in one of the interfaces present in the orthorhombic Spc-SH3 crystal structures. In order to understand the molecular interactions that are responsible for the very fast crystal growth of the wild-type (WT) Spc-SH3 crystals, the crystal structure of a triple mutant in which the residues Ser19-Pro20-Arg21 in the RT loop have been replaced by Gly19-Asp20-Ser21 (GDS Spc-SH3 mutant) has been solved. The removal of the critical proline residue results in slower nucleation of the Spc-SH3 crystals and a different arrangement of the protein molecules in the unit cell, leading to a crystal that belongs to the tetragonal space group P4(1)2(1)2, with unit-cell parameters a = b = 42.231, c = 93.655 A, and that diffracts to 1.45 A resolution. For both WT Spc-SH3 and the GDS mutant, light-scattering experiments showed that a dimer was formed in solution within a few minutes of the addition of 2 M ammonium sulfate at pH 6.5 and allowed the proposal of a mechanism for the nucleation and crystal growth of Spc-SH3 in which the Pro20 residue plays a key role in the rate of crystal growth.	+
-		+
-	The effect of a proline residue on the rate of growth and the space group of alpha-spectrin SH3-domain crystals.,Camara-Artigas A, Andujar-Sanchez M, Ortiz-Salmeron E, Cuadri C, Casares S Acta Crystallogr D Biol Crystallogr. 2009 Dec;65(Pt 12):1247-52. Epub 2009, Nov 17. PMID:19966410<ref>PMID:19966410</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3m0u" style="background-color:#fffaf0;"></div>~~	+

	==See Also==		==See Also==
-	*[[Spectrin\|Spectrin]]	+	*[[Spectrin 3D structures\|Spectrin 3D structures]]
-	~~== References ==~~	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Chick~~]]	+	[[Category: Gallus gallus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Camara-Artigas, A]]	+	[[Category: Camara-Artigas A]]
-	[[Category: Gavira~~, J A]]~~	+	[[Category: Gavira JA]]
-	~~[[Category: Actin capping]]~~	+
-	~~[[Category: Actin-binding]]~~	+
-	~~[[Category: Calmodulin-binding]]~~	+
-	~~[[Category: Cytoskeleton]]~~	+
-	~~[[Category: Phosphoprotein]]~~	+
-	~~[[Category: Sh3 domain]]~~	+
-	~~[[Category: Sh3-like barrel]]~~	+
-	~~[[Category: Signaling protein~~]]	+

OCA at 12:41, 13 October 2021

OCA — Wed, 13 Oct 2021 12:41:04 GMT

←Older revision		Revision as of 12:41, 13 October 2021
Line 1:		Line 1:

	==Crystal Structure of the R21D mutant of alpha-spectrin SH3 domain. Hexagonal crystal obtained in sodium formate at pH 6.5.==		==Crystal Structure of the R21D mutant of alpha-spectrin SH3 domain. Hexagonal crystal obtained in sodium formate at pH 6.5.==
-	<StructureSection load='3m0u' size='340' side='right' caption='[[3m0u]], [[Resolution\|resolution]] 1.10Å' scene=''>	+	<StructureSection load='3m0u' size='340' side='right'caption='[[3m0u]], [[Resolution\|resolution]] 1.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3m0u]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Chick Chick]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M0U OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3M0U FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3m0u]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Chick Chick]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M0U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3M0U FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1shg\|1shg]], [[3i9q\|3i9q]], [[2f2w\|2f2w]], [[2f2x\|2f2x]], [[3m0p\|3m0p]], [[3m0q\|3m0q]], [[3m0r\|3m0r]], [[3m0s\|3m0s]], [[3m0t\|3m0t]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1shg\|1shg]], [[3i9q\|3i9q]], [[2f2w\|2f2w]], [[2f2x\|2f2x]], [[3m0p\|3m0p]], [[3m0q\|3m0q]], [[3m0r\|3m0r]], [[3m0s\|3m0s]], [[3m0t\|3m0t]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SPTAN1, SPTA2 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 CHICK])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SPTAN1, SPTA2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 CHICK])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3m0u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m0u OCA], [~~http~~://pdbe.org/3m0u PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3m0u RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3m0u PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3m0u ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3m0u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m0u OCA], [https://pdbe.org/3m0u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3m0u RCSB], [https://www.ebi.ac.uk/pdbsum/3m0u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3m0u ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/SPTN1_CHICK SPTN1_CHICK]] Morphologically, spectrin-like proteins appear to be related to spectrin, showing a flexible rod-like structure. They can bind actin but seem to differ in their calmodulin-binding activity. In nonerythroid tissues, spectrins, in association with some other proteins, may play an important role in membrane organization.	+	[[https://www.uniprot.org/uniprot/SPTN1_CHICK SPTN1_CHICK]] Morphologically, spectrin-like proteins appear to be related to spectrin, showing a flexible rod-like structure. They can bind actin but seem to differ in their calmodulin-binding activity. In nonerythroid tissues, spectrins, in association with some other proteins, may play an important role in membrane organization.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 28:		Line 28:
	</StructureSection>		</StructureSection>
	[[Category: Chick]]		[[Category: Chick]]
		+	[[Category: Large Structures]]
	[[Category: Camara-Artigas, A]]		[[Category: Camara-Artigas, A]]
	[[Category: Gavira, J A]]		[[Category: Gavira, J A]]

OCA at 17:51, 5 August 2016

OCA — Fri, 05 Aug 2016 17:51:15 GMT

←Older revision		Revision as of 17:51, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal Structure of the R21D mutant of alpha-spectrin SH3 domain. Hexagonal crystal obtained in sodium formate at pH 6.5.==		==Crystal Structure of the R21D mutant of alpha-spectrin SH3 domain. Hexagonal crystal obtained in sodium formate at pH 6.5.==
	<StructureSection load='3m0u' size='340' side='right' caption='[[3m0u]], [[Resolution\|resolution]] 1.10Å' scene=''>		<StructureSection load='3m0u' size='340' side='right' caption='[[3m0u]], [[Resolution\|resolution]] 1.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3m0u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Gallus_gallus Gallus gallus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M0U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3M0U FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3m0u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chick Chick]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M0U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3M0U FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1shg\|1shg]], [[3i9q\|3i9q]], [[2f2w\|2f2w]], [[2f2x\|2f2x]], [[3m0p\|3m0p]], [[3m0q\|3m0q]], [[3m0r\|3m0r]], [[3m0s\|3m0s]], [[3m0t\|3m0t]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1shg\|1shg]], [[3i9q\|3i9q]], [[2f2w\|2f2w]], [[2f2x\|2f2x]], [[3m0p\|3m0p]], [[3m0q\|3m0q]], [[3m0r\|3m0r]], [[3m0s\|3m0s]], [[3m0t\|3m0t]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SPTAN1, SPTA2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 ~~Gallus gallus~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SPTAN1, SPTA2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 CHICK])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3m0u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m0u OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3m0u RCSB], [http://www.ebi.ac.uk/pdbsum/3m0u PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3m0u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m0u OCA], [http://pdbe.org/3m0u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3m0u RCSB], [http://www.ebi.ac.uk/pdbsum/3m0u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3m0u ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3m0u" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 25:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Gallus gallus~~]]	+	[[Category: Chick]]
	[[Category: Camara-Artigas, A]]		[[Category: Camara-Artigas, A]]
	[[Category: Gavira, J A]]		[[Category: Gavira, J A]]

OCA at 04:35, 25 December 2014

OCA — Thu, 25 Dec 2014 04:35:28 GMT

←Older revision		Revision as of 04:35, 25 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3m0u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m0u OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3m0u RCSB], [http://www.ebi.ac.uk/pdbsum/3m0u PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3m0u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m0u OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3m0u RCSB], [http://www.ebi.ac.uk/pdbsum/3m0u PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SPTN1_CHICK SPTN1_CHICK]] Morphologically, spectrin-like proteins appear to be related to spectrin, showing a flexible rod-like structure. They can bind actin but seem to differ in their calmodulin-binding activity. In nonerythroid tissues, spectrins, in association with some other proteins, may play an important role in membrane organization.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 17:51, 18 December 2014

OCA — Thu, 18 Dec 2014 17:51:30 GMT

←Older revision		Revision as of 17:51, 18 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3m0u\| PDB=3m0u \| SCENE= }}~~	+	==Crystal Structure of the R21D mutant of alpha-spectrin SH3 domain. Hexagonal crystal obtained in sodium formate at pH 6.5.==
-	===Crystal Structure of the R21D mutant of alpha-spectrin SH3 domain. Hexagonal crystal obtained in sodium formate at pH 6.5.===	+	<StructureSection load='3m0u' size='340' side='right' caption='[[3m0u]], [[Resolution\|resolution]] 1.10Å' scene=''>
-	~~{{ABSTRACT_PUBMED_19966410}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3m0u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M0U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3M0U FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1shg\|1shg]], [[3i9q\|3i9q]], [[2f2w\|2f2w]], [[2f2x\|2f2x]], [[3m0p\|3m0p]], [[3m0q\|3m0q]], [[3m0r\|3m0r]], [[3m0s\|3m0s]], [[3m0t\|3m0t]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SPTAN1, SPTA2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 Gallus gallus])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3m0u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m0u OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3m0u RCSB], [http://www.ebi.ac.uk/pdbsum/3m0u PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	alpha-Spectrin SH3-domain (Spc-SH3) crystallization is characterized by very fast growth of the crystals in the presence of ammonium sulfate as a precipitant agent. The origin of this behaviour can be attributed to the presence of a proline residue that participates in a crystal contact mimicking the binding of proline-rich sequences to SH3 domains. This residue, Pro20, is located in the RT loop and is the main contact in one of the interfaces present in the orthorhombic Spc-SH3 crystal structures. In order to understand the molecular interactions that are responsible for the very fast crystal growth of the wild-type (WT) Spc-SH3 crystals, the crystal structure of a triple mutant in which the residues Ser19-Pro20-Arg21 in the RT loop have been replaced by Gly19-Asp20-Ser21 (GDS Spc-SH3 mutant) has been solved. The removal of the critical proline residue results in slower nucleation of the Spc-SH3 crystals and a different arrangement of the protein molecules in the unit cell, leading to a crystal that belongs to the tetragonal space group P4(1)2(1)2, with unit-cell parameters a = b = 42.231, c = 93.655 A, and that diffracts to 1.45 A resolution. For both WT Spc-SH3 and the GDS mutant, light-scattering experiments showed that a dimer was formed in solution within a few minutes of the addition of 2 M ammonium sulfate at pH 6.5 and allowed the proposal of a mechanism for the nucleation and crystal growth of Spc-SH3 in which the Pro20 residue plays a key role in the rate of crystal growth.

-	~~==Function==~~	+	The effect of a proline residue on the rate of growth and the space group of alpha-spectrin SH3-domain crystals.,Camara-Artigas A, Andujar-Sanchez M, Ortiz-Salmeron E, Cuadri C, Casares S Acta Crystallogr D Biol Crystallogr. 2009 Dec;65(Pt 12):1247-52. Epub 2009, Nov 17. PMID:19966410<ref>PMID:19966410</ref>
-	~~[[http://www~~.~~uniprot.org/uniprot/SPTN1_CHICK SPTN1_CHICK]] Morphologically~~, ~~spectrin~~-~~like proteins appear to be related to spectrin~~, ~~showing a flexible rod~~-~~like structure~~. ~~They can bind actin but seem to differ in their calmodulin~~-~~binding activity~~. ~~In nonerythroid tissues~~, ~~spectrins, in association with some other proteins, may play an important role in membrane organization~~.	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[3m0u]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Gallus_gallus Gallus gallus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3M0U OCA].~~	+	</div>

	==See Also==		==See Also==
	*[[Spectrin\|Spectrin]]		*[[Spectrin\|Spectrin]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:019966410</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Gallus gallus]]		[[Category: Gallus gallus]]
-	[[Category: Camara-Artigas, A.]]	+	[[Category: Camara-Artigas, A]]
-	[[Category: Gavira, J A.]]	+	[[Category: Gavira, J A]]
	[[Category: Actin capping]]		[[Category: Actin capping]]
	[[Category: Actin-binding]]		[[Category: Actin-binding]]

OCA at 06:28, 6 November 2013

OCA — Wed, 06 Nov 2013 06:28:40 GMT

←Older revision		Revision as of 06:28, 6 November 2013
Line 1:		Line 1:
-	[[Image:3m0u.png\|left\|200px]]
-
	{{STRUCTURE_3m0u\| PDB=3m0u \| SCENE= }}		{{STRUCTURE_3m0u\| PDB=3m0u \| SCENE= }}
-
	===Crystal Structure of the R21D mutant of alpha-spectrin SH3 domain. Hexagonal crystal obtained in sodium formate at pH 6.5.===		===Crystal Structure of the R21D mutant of alpha-spectrin SH3 domain. Hexagonal crystal obtained in sodium formate at pH 6.5.===
		+	{{ABSTRACT_PUBMED_19966410}}

-	~~{{ABSTRACT_PUBMED_19966410}}~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/SPTN1_CHICK SPTN1_CHICK]] Morphologically, spectrin-like proteins appear to be related to spectrin, showing a flexible rod-like structure. They can bind actin but seem to differ in their calmodulin-binding activity. In nonerythroid tissues, spectrins, in association with some other proteins, may play an important role in membrane organization.

	==About this Structure==		==About this Structure==
Line 14:		Line 13:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:019966410</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019966410</ref><references group="xtra"/><references/>
	[[Category: Gallus gallus]]		[[Category: Gallus gallus]]
	[[Category: Camara-Artigas, A.]]		[[Category: Camara-Artigas, A.]]

OCA at 12:43, 20 October 2012

OCA — Sat, 20 Oct 2012 12:43:10 GMT

←Older revision		Revision as of 12:43, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3m0u]] is a 1 chain structure ~~of [[Spectrin]]~~ with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M0U OCA].	+	[[3m0u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M0U OCA].

	==See Also==		==See Also==

OCA at 09:38, 26 July 2012

OCA — Thu, 26 Jul 2012 09:38:47 GMT

←Older revision		Revision as of 09:38, 26 July 2012
Line 1:		Line 1:
-	[[Image:3m0u.~~jpg~~\|left\|200px]]	+	[[Image:3m0u.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3m0u", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3m0u\| PDB=3m0u \| SCENE= }}		{{STRUCTURE_3m0u\| PDB=3m0u \| SCENE= }}

	===Crystal Structure of the R21D mutant of alpha-spectrin SH3 domain. Hexagonal crystal obtained in sodium formate at pH 6.5.===		===Crystal Structure of the R21D mutant of alpha-spectrin SH3 domain. Hexagonal crystal obtained in sodium formate at pH 6.5.===

		+	{{ABSTRACT_PUBMED_19966410}}

	==About this Structure==		==About this Structure==
-	[[3m0u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M0U OCA].	+	[[3m0u]] is a 1 chain structure of [[Spectrin]] with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M0U OCA].
		+
		+	==See Also==
		+	*[[Spectrin\|Spectrin]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~19966410~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019966410</ref><references group="xtra"/>
	[[Category: Gallus gallus]]		[[Category: Gallus gallus]]
	[[Category: Camara-Artigas, A.]]		[[Category: Camara-Artigas, A.]]
	[[Category: Gavira, J A.]]		[[Category: Gavira, J A.]]
		+	[[Category: Actin capping]]
		+	[[Category: Actin-binding]]
		+	[[Category: Calmodulin-binding]]
		+	[[Category: Cytoskeleton]]
		+	[[Category: Phosphoprotein]]
		+	[[Category: Sh3 domain]]
		+	[[Category: Sh3-like barrel]]
		+	[[Category: Signaling protein]]

OCA: Protected "3m0u" [edit=sysop:move=sysop]

OCA — Wed, 19 Jan 2011 06:22:25 GMT

Protected "3m0u" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:22, 19 January 2011

OCA at 06:22, 19 January 2011

OCA — Wed, 19 Jan 2011 06:22:20 GMT

←Older revision		Revision as of 06:22, 19 January 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3m0u.jpg\|left\|200px]]

-	The ~~entry 3m0u~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3m0u", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3m0u\| PDB=3m0u \| SCENE= }}

-	~~Authors: Gavira, J~~.A.~~, Camara-Artigas, A~~.	+	===Crystal Structure of the R21D mutant of alpha-spectrin SH3 domain. Hexagonal crystal obtained in sodium formate at pH 6.5.===

-	Description: Crystal Structure of the R21D mutant of alpha-spectrin SH3 domain. Hexagonal crystal obtained in sodium formate at pH 6.5.

-	~~''Page seeded by~~ [http://oca.weizmann.ac.il/oca OCA ] ~~on Wed Apr 14 09~~:21:~~17 2010''~~	+	==About this Structure==
		+	[[3m0u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M0U OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:19966410</ref><references group="xtra"/>
		+	[[Category: Gallus gallus]]
		+	[[Category: Camara-Artigas, A.]]
		+	[[Category: Gavira, J A.]]