3mjx - Revision history

OCA at 14:05, 13 March 2024

OCA — Wed, 13 Mar 2024 14:05:47 GMT

←Older revision		Revision as of 14:05, 13 March 2024
Line 10:		Line 10:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/MYS2_DICDI MYS2_DICDI] Myosin is a protein that binds to actin and has ATPase activity that is activated by actin.		[https://www.uniprot.org/uniprot/MYS2_DICDI MYS2_DICDI] Myosin is a protein that binds to actin and has ATPase activity that is activated by actin.
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	We have identified pentabromopseudilin (PBP) as a potent inhibitor of myosin-dependent processes such as isometric tension development and unloaded shortening velocity. PBP-induced reductions in the rate constants for ATP binding, ATP hydrolysis and ADP dissociation extend the time required per myosin ATPase cycle in the absence and presence of actin. Additionally, coupling between the actin and nucleotide binding sites is reduced in the presence of the inhibitor. The selectivity of PBP differs from that observed with other myosin inhibitors. To elucidate the binding mode of PBP, we crystallized the Dictyostelium myosin-2 motor domain in the presence of Mg(2+)-ADP-meta-vanadate and PBP. The electron density for PBP is unambiguous and shows PBP to bind at a previously unknown allosteric site near the tip of the 50-kDa domain, at a distance of 16 A from the nucleotide binding site and 7.5 A away from the blebbistatin binding pocket.
-
-	The mechanism of pentabromopseudilin inhibition of myosin motor activity.,Fedorov R, Bohl M, Tsiavaliaris G, Hartmann FK, Taft MH, Baruch P, Brenner B, Martin R, Knolker HJ, Gutzeit HO, Manstein DJ Nat Struct Mol Biol. 2009 Jan;16(1):80-8. Epub 2009 Jan 4. PMID:19122661<ref>PMID:19122661</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3mjx" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Myosin 3D Structures\|Myosin 3D Structures]]		*[[Myosin 3D Structures\|Myosin 3D Structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 08:54, 6 September 2023

OCA — Wed, 06 Sep 2023 08:54:58 GMT

←Older revision		Revision as of 08:54, 6 September 2023
Line 3:		Line 3:
	<StructureSection load='3mjx' size='340' side='right'caption='[[3mjx]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='3mjx' size='340' side='right'caption='[[3mjx]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3mjx]] is a 1 chain structure with sequence from ~~[https://en.wikipedia.org/wiki/Dicdi Dicdi] and~~ [https://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MJX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3MJX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3mjx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MJX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3MJX FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AD9:ADP+METAVANADATE'>AD9</scene>, <scene name='pdbligand=BIT:(-)-1-PHENYL-1,2,3,4-TETRAHYDRO-4-HYDROXYPYRROLO[2,3-B]-7-METHYLQUINOLIN-4-ONE'>BIT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2jhr\|2jhr]], [[2jj9\|2jj9]], [[3bz7\|3bz7]], [[3bz8\|3bz8]], [[3bz9\|3bz9]], [[1yv3\|1yv3]]</div></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AD9:ADP+METAVANADATE'>AD9</scene>, <scene name='pdbligand=BIT:(-)-1-PHENYL-1,2,3,4-TETRAHYDRO-4-HYDROXYPYRROLO[2,3-B]-7-METHYLQUINOLIN-4-ONE'>BIT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mhcA, DDB_G0286355 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=44689 DICDI])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3mjx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mjx OCA], [https://pdbe.org/3mjx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3mjx RCSB], [https://www.ebi.ac.uk/pdbsum/3mjx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3mjx ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3mjx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mjx OCA], [https://pdbe.org/3mjx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3mjx RCSB], [https://www.ebi.ac.uk/pdbsum/3mjx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3mjx ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/MYS2_DICDI MYS2_DICDI]] Myosin is a protein that binds to actin and has ATPase activity that is activated by actin.	+	[https://www.uniprot.org/uniprot/MYS2_DICDI MYS2_DICDI] Myosin is a protein that binds to actin and has ATPase activity that is activated by actin.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 27:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Dicdi]]
	[[Category: Dictyostelium discoideum]]		[[Category: Dictyostelium discoideum]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Baruch, P]]	+	[[Category: Baruch P]]
-	[[Category: Bauer, S]]	+	[[Category: Bauer S]]
-	[[Category: Fedorov, R]]	+	[[Category: Fedorov R]]
-	[[Category: Manstein~~, D J]]~~	+	[[Category: Manstein DJ]]
-	~~[[Category: Actin-binding]]~~	+
-	~~[[Category: Adp-metavanadate]]~~	+
-	~~[[Category: Allosteric]]~~	+
-	~~[[Category: Atp-binding]]~~	+
-	~~[[Category: Blebbistatin]]~~	+
-	~~[[Category: Calmodulin-binding]]~~	+
-	~~[[Category: Contractile protein]]~~	+
-	~~[[Category: Methylation]]~~	+
-	~~[[Category: Motor protein]]~~	+
-	~~[[Category: Myosin]]~~	+
-	~~[[Category: Nucleotide-binding]]~~	+
-	~~[[Category: Phosphoprotein]]~~	+
-	~~[[Category: Structural protein~~]]	+

OCA at 13:47, 4 May 2022

OCA — Wed, 04 May 2022 13:47:31 GMT

←Older revision		Revision as of 13:47, 4 May 2022
Line 1:		Line 1:

	==Crystal structure of myosin-2 motor domain in complex with ADP-Metavanadate and blebbistatin==		==Crystal structure of myosin-2 motor domain in complex with ADP-Metavanadate and blebbistatin==
-	<StructureSection load='3mjx' size='340' side='right' caption='[[3mjx]], [[Resolution\|resolution]] 2.20Å' scene=''>	+	<StructureSection load='3mjx' size='340' side='right'caption='[[3mjx]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3mjx]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Dicdi Dicdi] and [~~http~~://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MJX OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3MJX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3mjx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Dicdi Dicdi] and [https://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MJX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3MJX FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AD9:ADP+METAVANADATE'>AD9</scene>, <scene name='pdbligand=BIT:(-)-1-PHENYL-1,2,3,4-TETRAHYDRO-4-HYDROXYPYRROLO[2,3-B]-7-METHYLQUINOLIN-4-ONE'>BIT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AD9:ADP+METAVANADATE'>AD9</scene>, <scene name='pdbligand=BIT:(-)-1-PHENYL-1,2,3,4-TETRAHYDRO-4-HYDROXYPYRROLO[2,3-B]-7-METHYLQUINOLIN-4-ONE'>BIT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2jhr\|2jhr]], [[2jj9\|2jj9]], [[3bz7\|3bz7]], [[3bz8\|3bz8]], [[3bz9\|3bz9]], [[1yv3\|1yv3]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2jhr\|2jhr]], [[2jj9\|2jj9]], [[3bz7\|3bz7]], [[3bz8\|3bz8]], [[3bz9\|3bz9]], [[1yv3\|1yv3]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mhcA, DDB_G0286355 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=44689 DICDI])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mhcA, DDB_G0286355 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=44689 DICDI])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3mjx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mjx OCA], [~~http~~://pdbe.org/3mjx PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3mjx RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3mjx PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3mjx ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3mjx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mjx OCA], [https://pdbe.org/3mjx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3mjx RCSB], [https://www.ebi.ac.uk/pdbsum/3mjx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3mjx ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/MYS2_DICDI MYS2_DICDI]] Myosin is a protein that binds to actin and has ATPase activity that is activated by actin.	+	[[https://www.uniprot.org/uniprot/MYS2_DICDI MYS2_DICDI]] Myosin is a protein that binds to actin and has ATPase activity that is activated by actin.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:

	==See Also==		==See Also==
-	*[[Myosin\|Myosin]]	+	*[[Myosin 3D Structures\|Myosin 3D Structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 29:		Line 29:
	[[Category: Dicdi]]		[[Category: Dicdi]]
	[[Category: Dictyostelium discoideum]]		[[Category: Dictyostelium discoideum]]
		+	[[Category: Large Structures]]
	[[Category: Baruch, P]]		[[Category: Baruch, P]]
	[[Category: Bauer, S]]		[[Category: Bauer, S]]

OCA at 13:32, 16 January 2019

OCA — Wed, 16 Jan 2019 13:32:06 GMT

←Older revision		Revision as of 13:32, 16 January 2019
Line 3:		Line 3:
	<StructureSection load='3mjx' size='340' side='right' caption='[[3mjx]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='3mjx' size='340' side='right' caption='[[3mjx]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3mjx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Atcc_11735 Atcc 11735~~] and [http://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MJX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3MJX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3mjx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dicdi Dicdi] and [http://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MJX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3MJX FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AD9:ADP+METAVANADATE'>AD9</scene>, <scene name='pdbligand=BIT:(-)-1-PHENYL-1,2,3,4-TETRAHYDRO-4-HYDROXYPYRROLO[2,3-B]-7-METHYLQUINOLIN-4-ONE'>BIT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AD9:ADP+METAVANADATE'>AD9</scene>, <scene name='pdbligand=BIT:(-)-1-PHENYL-1,2,3,4-TETRAHYDRO-4-HYDROXYPYRROLO[2,3-B]-7-METHYLQUINOLIN-4-ONE'>BIT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2jhr\|2jhr]], [[2jj9\|2jj9]], [[3bz7\|3bz7]], [[3bz8\|3bz8]], [[3bz9\|3bz9]], [[1yv3\|1yv3]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2jhr\|2jhr]], [[2jj9\|2jj9]], [[3bz7\|3bz7]], [[3bz8\|3bz8]], [[3bz9\|3bz9]], [[1yv3\|1yv3]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mhcA, DDB_G0286355 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=44689 ~~ATCC 11735~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mhcA, DDB_G0286355 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=44689 DICDI])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3mjx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mjx OCA], [http://pdbe.org/3mjx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3mjx RCSB], [http://www.ebi.ac.uk/pdbsum/3mjx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3mjx ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3mjx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mjx OCA], [http://pdbe.org/3mjx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3mjx RCSB], [http://www.ebi.ac.uk/pdbsum/3mjx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3mjx ProSAT]</span></td></tr>
	</table>		</table>
Line 27:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Atcc 11735~~]]	+	[[Category: Dicdi]]
	[[Category: Dictyostelium discoideum]]		[[Category: Dictyostelium discoideum]]
	[[Category: Baruch, P]]		[[Category: Baruch, P]]

OCA at 16:28, 4 August 2016

OCA — Thu, 04 Aug 2016 16:28:29 GMT

←Older revision		Revision as of 16:28, 4 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of myosin-2 motor domain in complex with ADP-Metavanadate and blebbistatin==		==Crystal structure of myosin-2 motor domain in complex with ADP-Metavanadate and blebbistatin==
	<StructureSection load='3mjx' size='340' side='right' caption='[[3mjx]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='3mjx' size='340' side='right' caption='[[3mjx]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3mjx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MJX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3MJX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3mjx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_11735 Atcc 11735] and [http://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MJX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3MJX FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AD9:ADP+METAVANADATE'>AD9</scene>, <scene name='pdbligand=BIT:(-)-1-PHENYL-1,2,3,4-TETRAHYDRO-4-HYDROXYPYRROLO[2,3-B]-7-METHYLQUINOLIN-4-ONE'>BIT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AD9:ADP+METAVANADATE'>AD9</scene>, <scene name='pdbligand=BIT:(-)-1-PHENYL-1,2,3,4-TETRAHYDRO-4-HYDROXYPYRROLO[2,3-B]-7-METHYLQUINOLIN-4-ONE'>BIT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2jhr\|2jhr]], [[2jj9\|2jj9]], [[3bz7\|3bz7]], [[3bz8\|3bz8]], [[3bz9\|3bz9]], [[1yv3\|1yv3]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2jhr\|2jhr]], [[2jj9\|2jj9]], [[3bz7\|3bz7]], [[3bz8\|3bz8]], [[3bz9\|3bz9]], [[1yv3\|1yv3]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mhcA, DDB_G0286355 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=44689 ~~Dictyostelium discoideum~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mhcA, DDB_G0286355 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=44689 ATCC 11735])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3mjx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mjx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3mjx RCSB], [http://www.ebi.ac.uk/pdbsum/3mjx PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3mjx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mjx OCA], [http://pdbe.org/3mjx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3mjx RCSB], [http://www.ebi.ac.uk/pdbsum/3mjx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3mjx ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3mjx" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 25:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 11735]]
	[[Category: Dictyostelium discoideum]]		[[Category: Dictyostelium discoideum]]
	[[Category: Baruch, P]]		[[Category: Baruch, P]]

OCA at 10:59, 25 December 2014

OCA — Thu, 25 Dec 2014 10:59:28 GMT

←Older revision		Revision as of 10:59, 25 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3mjx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mjx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3mjx RCSB], [http://www.ebi.ac.uk/pdbsum/3mjx PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3mjx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mjx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3mjx RCSB], [http://www.ebi.ac.uk/pdbsum/3mjx PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MYS2_DICDI MYS2_DICDI]] Myosin is a protein that binds to actin and has ATPase activity that is activated by actin.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 09:41, 9 December 2014

OCA — Tue, 09 Dec 2014 09:41:56 GMT

←Older revision		Revision as of 09:41, 9 December 2014
Line 1:		Line 1:
-	[[~~Image:~~3mjx.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of myosin-2 motor domain in complex with ADP-Metavanadate and blebbistatin==
		+	<StructureSection load='3mjx' size='340' side='right' caption='[[3mjx]], [[Resolution\|resolution]] 2.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3mjx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MJX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3MJX FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AD9:ADP+METAVANADATE'>AD9</scene>, <scene name='pdbligand=BIT:(-)-1-PHENYL-1,2,3,4-TETRAHYDRO-4-HYDROXYPYRROLO[2,3-B]-7-METHYLQUINOLIN-4-ONE'>BIT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2jhr\|2jhr]], [[2jj9\|2jj9]], [[3bz7\|3bz7]], [[3bz8\|3bz8]], [[3bz9\|3bz9]], [[1yv3\|1yv3]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mhcA, DDB_G0286355 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=44689 Dictyostelium discoideum])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3mjx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mjx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3mjx RCSB], [http://www.ebi.ac.uk/pdbsum/3mjx PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	We have identified pentabromopseudilin (PBP) as a potent inhibitor of myosin-dependent processes such as isometric tension development and unloaded shortening velocity. PBP-induced reductions in the rate constants for ATP binding, ATP hydrolysis and ADP dissociation extend the time required per myosin ATPase cycle in the absence and presence of actin. Additionally, coupling between the actin and nucleotide binding sites is reduced in the presence of the inhibitor. The selectivity of PBP differs from that observed with other myosin inhibitors. To elucidate the binding mode of PBP, we crystallized the Dictyostelium myosin-2 motor domain in the presence of Mg(2+)-ADP-meta-vanadate and PBP. The electron density for PBP is unambiguous and shows PBP to bind at a previously unknown allosteric site near the tip of the 50-kDa domain, at a distance of 16 A from the nucleotide binding site and 7.5 A away from the blebbistatin binding pocket.

-	~~{{STRUCTURE_3mjx\| PDB=3mjx \| SCENE= }}~~	+	The mechanism of pentabromopseudilin inhibition of myosin motor activity.,Fedorov R, Bohl M, Tsiavaliaris G, Hartmann FK, Taft MH, Baruch P, Brenner B, Martin R, Knolker HJ, Gutzeit HO, Manstein DJ Nat Struct Mol Biol. 2009 Jan;16(1):80-8. Epub 2009 Jan 4. PMID:19122661<ref>PMID:19122661</ref>

-	~~===Crystal structure~~ of ~~myosin-2 motor domain in complex with ADP-Metavanadate and blebbistatin===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_19122661}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3mjx]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3MJX OCA].~~	+

	==See Also==		==See Also==
	*[[Myosin\|Myosin]]		*[[Myosin\|Myosin]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:019122661<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Dictyostelium discoideum]]		[[Category: Dictyostelium discoideum]]
-	[[Category: Baruch, P.]]	+	[[Category: Baruch, P]]
-	[[Category: Bauer, S.]]	+	[[Category: Bauer, S]]
-	[[Category: Fedorov, R.]]	+	[[Category: Fedorov, R]]
-	[[Category: Manstein, D J.]]	+	[[Category: Manstein, D J]]
	[[Category: Actin-binding]]		[[Category: Actin-binding]]
	[[Category: Adp-metavanadate]]		[[Category: Adp-metavanadate]]

OCA at 15:54, 20 October 2012

OCA — Sat, 20 Oct 2012 15:54:42 GMT

←Older revision		Revision as of 15:54, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3mjx]] is a 1 chain structure ~~of [[Myosin]]~~ with sequence from [http://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MJX OCA].	+	[[3mjx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MJX OCA].

	==See Also==		==See Also==

OCA at 19:53, 26 July 2012

OCA — Thu, 26 Jul 2012 19:53:18 GMT

←Older revision		Revision as of 19:53, 26 July 2012
Line 1:		Line 1:
-	[[Image:3mjx.~~jpg~~\|left\|200px]]	+	[[Image:3mjx.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3mjx", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3mjx\| PDB=3mjx \| SCENE= }}		{{STRUCTURE_3mjx\| PDB=3mjx \| SCENE= }}

	===Crystal structure of myosin-2 motor domain in complex with ADP-Metavanadate and blebbistatin===		===Crystal structure of myosin-2 motor domain in complex with ADP-Metavanadate and blebbistatin===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_19122661}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 19122661 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_19122661}}		{{ABSTRACT_PUBMED_19122661}}

	==About this Structure==		==About this Structure==
-	[[3mjx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MJX OCA].	+	[[3mjx]] is a 1 chain structure of [[Myosin]] with sequence from [http://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MJX OCA].
		+
		+	==See Also==
		+	*[[Myosin\|Myosin]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~19122661~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019122661</ref><references group="xtra"/>
	[[Category: Dictyostelium discoideum]]		[[Category: Dictyostelium discoideum]]
	[[Category: Baruch, P.]]		[[Category: Baruch, P.]]
Line 28:		Line 20:
	[[Category: Fedorov, R.]]		[[Category: Fedorov, R.]]
	[[Category: Manstein, D J.]]		[[Category: Manstein, D J.]]
		+	[[Category: Actin-binding]]
		+	[[Category: Adp-metavanadate]]
		+	[[Category: Allosteric]]
		+	[[Category: Atp-binding]]
		+	[[Category: Blebbistatin]]
		+	[[Category: Calmodulin-binding]]
		+	[[Category: Contractile protein]]
		+	[[Category: Methylation]]
		+	[[Category: Motor protein]]
		+	[[Category: Myosin]]
		+	[[Category: Nucleotide-binding]]
		+	[[Category: Phosphoprotein]]
		+	[[Category: Structural protein]]

OCA: Protected "3mjx" [edit=sysop:move=sysop]

OCA — Wed, 27 Apr 2011 07:17:12 GMT

Protected "3mjx" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:17, 27 April 2011