3n5i - Revision history

OCA at 09:09, 6 September 2023

OCA — Wed, 06 Sep 2023 09:09:10 GMT

←Older revision		Revision as of 09:09, 6 September 2023
Line 3:		Line 3:
	<StructureSection load='3n5i' size='340' side='right'caption='[[3n5i]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='3n5i' size='340' side='right'caption='[[3n5i]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3n5i]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Ccug_52537 Ccug 52537~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N5I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3N5I FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3n5i]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Sphingosinicella_xenopeptidilytica Sphingosinicella xenopeptidilytica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N5I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3N5I FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~, ~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[3n2w\|3n2w]], [[3n33\|3n33]]~~</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">BapA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=364098 CCUG 52537])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3n5i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n5i OCA], [https://pdbe.org/3n5i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3n5i RCSB], [https://www.ebi.ac.uk/pdbsum/3n5i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3n5i ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3n5i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n5i OCA], [https://pdbe.org/3n5i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3n5i RCSB], [https://www.ebi.ac.uk/pdbsum/3n5i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3n5i ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/BAPA_SPHXN BAPA_SPHXN]] Beta-aminopeptidase that can cleave synthetic beta-peptides which consist of backbone-elongated beta-amino acid residues that are not processed by common proteolytic enzymes. Can cleave the beta-peptides beta-homoVal-beta-homoAla-beta-homoLeu and beta-homoAla-beta-homoLeu. Requires a beta-amino acid at the N-terminus of peptide substrates and cleaves the peptide bond between the N-terminal beta-amino acid and the amino acid at the second position of tripeptidic substrates of the general structure H-betahXaa-Ile-betahTyr-OH according to the following preferences with regard to the side chain of the N-terminal beta-amino acid: aliphatic and aromatic > OH-containing > hydrogen, basic and polar.<ref>PMID:16109932</ref> <ref>PMID:17064315</ref>	+	[https://www.uniprot.org/uniprot/BAPA_SPHXN BAPA_SPHXN] Beta-aminopeptidase that can cleave synthetic beta-peptides which consist of backbone-elongated beta-amino acid residues that are not processed by common proteolytic enzymes. Can cleave the beta-peptides beta-homoVal-beta-homoAla-beta-homoLeu and beta-homoAla-beta-homoLeu. Requires a beta-amino acid at the N-terminus of peptide substrates and cleaves the peptide bond between the N-terminal beta-amino acid and the amino acid at the second position of tripeptidic substrates of the general structure H-betahXaa-Ile-betahTyr-OH according to the following preferences with regard to the side chain of the N-terminal beta-amino acid: aliphatic and aromatic > OH-containing > hydrogen, basic and polar.<ref>PMID:16109932</ref> <ref>PMID:17064315</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 27:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Ccug 52537]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Geueke, B]]	+	[[Category: Sphingosinicella xenopeptidilytica]]
-	[[Category: Gruetter~~, M G~~]]	+	[[Category: Geueke B]]
-	[[Category: Heck, T]]	+	[[Category: Gruetter MG]]
-	[[Category: Kohler, H P]]	+	[[Category: Heck T]]
-	[[Category: Merz, T]]	+	[[Category: Kohler H-P]]
-	~~[[Category: Alpha-beta-beta-alpha sandwich]]~~	+	[[Category: Merz T]]
-	~~[[Category: Beta-aminopeptidase]]~~	+
-	~~[[Category: Beta-peptide]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Ntn hydrolase~~]]	+

OCA at 06:55, 12 May 2022

OCA — Thu, 12 May 2022 06:55:27 GMT

←Older revision		Revision as of 06:55, 12 May 2022
Line 1:		Line 1:

	==Crystal structure of the precursor (S250A mutant) of the N-terminal beta-aminopeptidase BapA==		==Crystal structure of the precursor (S250A mutant) of the N-terminal beta-aminopeptidase BapA==
-	<StructureSection load='3n5i' size='340' side='right' caption='[[3n5i]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='3n5i' size='340' side='right'caption='[[3n5i]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3n5i]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Ccug_52537 Ccug 52537]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N5I OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3N5I FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3n5i]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Ccug_52537 Ccug 52537]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N5I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3N5I FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3n2w\|3n2w]], [[3n33\|3n33]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3n2w\|3n2w]], [[3n33\|3n33]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BapA ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=364098 CCUG 52537])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BapA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=364098 CCUG 52537])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3n5i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n5i OCA], [~~http~~://pdbe.org/3n5i PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3n5i RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3n5i PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3n5i ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3n5i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n5i OCA], [https://pdbe.org/3n5i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3n5i RCSB], [https://www.ebi.ac.uk/pdbsum/3n5i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3n5i ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/BAPA_SPHXN BAPA_SPHXN]] Beta-aminopeptidase that can cleave synthetic beta-peptides which consist of backbone-elongated beta-amino acid residues that are not processed by common proteolytic enzymes. Can cleave the beta-peptides beta-homoVal-beta-homoAla-beta-homoLeu and beta-homoAla-beta-homoLeu. Requires a beta-amino acid at the N-terminus of peptide substrates and cleaves the peptide bond between the N-terminal beta-amino acid and the amino acid at the second position of tripeptidic substrates of the general structure H-betahXaa-Ile-betahTyr-OH according to the following preferences with regard to the side chain of the N-terminal beta-amino acid: aliphatic and aromatic > OH-containing > hydrogen, basic and polar.<ref>PMID:16109932</ref> <ref>PMID:17064315</ref>	+	[[https://www.uniprot.org/uniprot/BAPA_SPHXN BAPA_SPHXN]] Beta-aminopeptidase that can cleave synthetic beta-peptides which consist of backbone-elongated beta-amino acid residues that are not processed by common proteolytic enzymes. Can cleave the beta-peptides beta-homoVal-beta-homoAla-beta-homoLeu and beta-homoAla-beta-homoLeu. Requires a beta-amino acid at the N-terminus of peptide substrates and cleaves the peptide bond between the N-terminal beta-amino acid and the amino acid at the second position of tripeptidic substrates of the general structure H-betahXaa-Ile-betahTyr-OH according to the following preferences with regard to the side chain of the N-terminal beta-amino acid: aliphatic and aromatic > OH-containing > hydrogen, basic and polar.<ref>PMID:16109932</ref> <ref>PMID:17064315</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:

	==See Also==		==See Also==
-	*[[Aminopeptidase\|Aminopeptidase]]	+	*[[Aminopeptidase 3D structures\|Aminopeptidase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 28:		Line 28:
	</StructureSection>		</StructureSection>
	[[Category: Ccug 52537]]		[[Category: Ccug 52537]]
		+	[[Category: Large Structures]]
	[[Category: Geueke, B]]		[[Category: Geueke, B]]
	[[Category: Gruetter, M G]]		[[Category: Gruetter, M G]]

OCA at 13:19, 16 January 2019

OCA — Wed, 16 Jan 2019 13:19:56 GMT

←Older revision		Revision as of 13:19, 16 January 2019
Line 3:		Line 3:
	<StructureSection load='3n5i' size='340' side='right' caption='[[3n5i]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='3n5i' size='340' side='right' caption='[[3n5i]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3n5i]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N5I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3N5I FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3n5i]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Ccug_52537 Ccug 52537]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N5I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3N5I FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3n2w\|3n2w]], [[3n33\|3n33]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3n2w\|3n2w]], [[3n33\|3n33]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BapA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=364098 CCUG 52537])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3n5i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n5i OCA], [http://pdbe.org/3n5i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3n5i RCSB], [http://www.ebi.ac.uk/pdbsum/3n5i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3n5i ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3n5i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n5i OCA], [http://pdbe.org/3n5i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3n5i RCSB], [http://www.ebi.ac.uk/pdbsum/3n5i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3n5i ProSAT]</span></td></tr>
	</table>		</table>
Line 26:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Ccug 52537]]
	[[Category: Geueke, B]]		[[Category: Geueke, B]]
	[[Category: Gruetter, M G]]		[[Category: Gruetter, M G]]

OCA at 07:28, 9 March 2017

OCA — Thu, 09 Mar 2017 07:28:23 GMT

←Older revision		Revision as of 07:28, 9 March 2017
Line 3:		Line 3:
	<StructureSection load='3n5i' size='340' side='right' caption='[[3n5i]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='3n5i' size='340' side='right' caption='[[3n5i]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3n5i]] is a 4 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Ccug_52537 Ccug 52537]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N5I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3N5I FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3n5i]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N5I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3N5I FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3n2w\|3n2w]], [[3n33\|3n33]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3n2w\|3n2w]], [[3n33\|3n33]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BapA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=364098 CCUG 52537])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3n5i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n5i OCA], [http://pdbe.org/3n5i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3n5i RCSB], [http://www.ebi.ac.uk/pdbsum/3n5i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3n5i ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3n5i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n5i OCA], [http://pdbe.org/3n5i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3n5i RCSB], [http://www.ebi.ac.uk/pdbsum/3n5i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3n5i ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/BAPA_SPHXN BAPA_SPHXN]] Beta-aminopeptidase that can cleave synthetic beta-peptides which consist of backbone-elongated beta-amino acid residues that are not processed by common proteolytic enzymes. Can cleave the beta-peptides beta-homoVal-beta-homoAla-beta-homoLeu and beta-homoAla-beta-homoLeu. Requires a beta-amino acid at the N-terminus of peptide substrates and cleaves the peptide bond between the N-terminal beta-amino acid and the amino acid at the second position of tripeptidic substrates of the general structure H-betahXaa-Ile-betahTyr-OH according to the following preferences with regard to the side chain of the N-terminal beta-amino acid: aliphatic and aromatic > OH-containing > hydrogen, basic and polar.<ref>PMID:16109932</ref> <ref>PMID:17064315</ref>		[[http://www.uniprot.org/uniprot/BAPA_SPHXN BAPA_SPHXN]] Beta-aminopeptidase that can cleave synthetic beta-peptides which consist of backbone-elongated beta-amino acid residues that are not processed by common proteolytic enzymes. Can cleave the beta-peptides beta-homoVal-beta-homoAla-beta-homoLeu and beta-homoAla-beta-homoLeu. Requires a beta-amino acid at the N-terminus of peptide substrates and cleaves the peptide bond between the N-terminal beta-amino acid and the amino acid at the second position of tripeptidic substrates of the general structure H-betahXaa-Ile-betahTyr-OH according to the following preferences with regard to the side chain of the N-terminal beta-amino acid: aliphatic and aromatic > OH-containing > hydrogen, basic and polar.<ref>PMID:16109932</ref> <ref>PMID:17064315</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The beta-aminopeptidase BapA from Sphingosinicella xenopeptidilytica belongs to the N-terminal nucleophile (Ntn) hydrolases of the DmpA-like family and has the unprecedented property of cleaving N-terminal beta-amino acid residues from peptides. We determined the crystal structures of the native (alphabeta)(4) heterooctamer and of the 153 kDa precursor homotetramer at a resolution of 1.45 and 1.8 A, respectively. These structures together with mutational analyses strongly support mechanisms for autoproteolysis and catalysis that involve residues Ser250, Ser288, and Glu290. The autoproteolytic mechanism is different from the one so far described for Ntn hydrolases. The structures together with functional data also provide insight into the discriminating features of the active site cleft that determine substrate specificity.
		+
		+	Autoproteolytic and catalytic mechanisms for the beta-aminopeptidase BapA--a member of the Ntn hydrolase family.,Merz T, Heck T, Geueke B, Mittl PR, Briand C, Seebach D, Kohler HP, Grutter MG Structure. 2012 Nov 7;20(11):1850-60. doi: 10.1016/j.str.2012.07.017. Epub 2012, Sep 12. PMID:22980995<ref>PMID:22980995</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 3n5i" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 18:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Ccug 52537]]
	[[Category: Geueke, B]]		[[Category: Geueke, B]]
	[[Category: Gruetter, M G]]		[[Category: Gruetter, M G]]

OCA at 05:52, 5 August 2016

OCA — Fri, 05 Aug 2016 05:52:58 GMT

←Older revision		Revision as of 05:52, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of the precursor (S250A mutant) of the N-terminal beta-aminopeptidase BapA==		==Crystal structure of the precursor (S250A mutant) of the N-terminal beta-aminopeptidase BapA==
	<StructureSection load='3n5i' size='340' side='right' caption='[[3n5i]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='3n5i' size='340' side='right' caption='[[3n5i]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3n5i]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Sphingosinicella_xenopeptidilytica Sphingosinicella xenopeptidilytica~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N5I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3N5I FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3n5i]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Ccug_52537 Ccug 52537]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N5I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3N5I FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3n2w\|3n2w]], [[3n33\|3n33]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3n2w\|3n2w]], [[3n33\|3n33]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BapA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=364098 ~~Sphingosinicella xenopeptidilytica~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BapA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=364098 CCUG 52537])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3n5i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n5i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3n5i RCSB], [http://www.ebi.ac.uk/pdbsum/3n5i PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3n5i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n5i OCA], [http://pdbe.org/3n5i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3n5i RCSB], [http://www.ebi.ac.uk/pdbsum/3n5i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3n5i ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/~~Q52VH2_9SPHN Q52VH2_9SPHN~~]] Beta-aminopeptidase that can cleave synthetic beta-peptides which consist of backbone-elongated beta-amino acid residues that are not processed by common proteolytic enzymes. Can cleave the beta-peptides beta-homoVal-beta-homoAla-beta-homoLeu and beta-homoAla-beta-homoLeu. Requires a beta-amino acid at the N-terminus of peptide substrates and cleaves the peptide bond between the N-terminal beta-amino acid and the amino acid at the second position of tripeptidic substrates of the general structure H-betahXaa-Ile-betahTyr-OH according to the following preferences with regard to the side chain of the N-terminal beta-amino acid: aliphatic and aromatic > OH-containing > hydrogen, basic and polar.<ref>PMID:16109932</ref> <ref>PMID:17064315</ref>	+	[[http://www.uniprot.org/uniprot/BAPA_SPHXN BAPA_SPHXN]] Beta-aminopeptidase that can cleave synthetic beta-peptides which consist of backbone-elongated beta-amino acid residues that are not processed by common proteolytic enzymes. Can cleave the beta-peptides beta-homoVal-beta-homoAla-beta-homoLeu and beta-homoAla-beta-homoLeu. Requires a beta-amino acid at the N-terminus of peptide substrates and cleaves the peptide bond between the N-terminal beta-amino acid and the amino acid at the second position of tripeptidic substrates of the general structure H-betahXaa-Ile-betahTyr-OH according to the following preferences with regard to the side chain of the N-terminal beta-amino acid: aliphatic and aromatic > OH-containing > hydrogen, basic and polar.<ref>PMID:16109932</ref> <ref>PMID:17064315</ref>

	==See Also==		==See Also==
Line 17:		Line 18:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Sphingosinicella xenopeptidilytica~~]]	+	[[Category: Ccug 52537]]
	[[Category: Geueke, B]]		[[Category: Geueke, B]]
	[[Category: Gruetter, M G]]		[[Category: Gruetter, M G]]

OCA at 16:17, 25 December 2014

OCA — Thu, 25 Dec 2014 16:17:30 GMT

←Older revision		Revision as of 16:17, 25 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3n5i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n5i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3n5i RCSB], [http://www.ebi.ac.uk/pdbsum/3n5i PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3n5i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n5i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3n5i RCSB], [http://www.ebi.ac.uk/pdbsum/3n5i PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/Q52VH2_9SPHN Q52VH2_9SPHN]] Beta-aminopeptidase that can cleave synthetic beta-peptides which consist of backbone-elongated beta-amino acid residues that are not processed by common proteolytic enzymes. Can cleave the beta-peptides beta-homoVal-beta-homoAla-beta-homoLeu and beta-homoAla-beta-homoLeu. Requires a beta-amino acid at the N-terminus of peptide substrates and cleaves the peptide bond between the N-terminal beta-amino acid and the amino acid at the second position of tripeptidic substrates of the general structure H-betahXaa-Ile-betahTyr-OH according to the following preferences with regard to the side chain of the N-terminal beta-amino acid: aliphatic and aromatic > OH-containing > hydrogen, basic and polar.<ref>PMID:16109932</ref> <ref>PMID:17064315</ref>

	==See Also==		==See Also==
	*[[Aminopeptidase\|Aminopeptidase]]		*[[Aminopeptidase\|Aminopeptidase]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 09:19, 9 December 2014

OCA — Tue, 09 Dec 2014 09:19:47 GMT

←Older revision		Revision as of 09:19, 9 December 2014
Line 1:		Line 1:
-	~~[[Image:3n5i.png\|left\|200px]]~~	+	==Crystal structure of the precursor (S250A mutant) of the N-terminal beta-aminopeptidase BapA==
-		+	<StructureSection load='3n5i' size='340' side='right' caption='[[3n5i]], [[Resolution\|resolution]] 1.80Å' scene=''>
-	~~{{STRUCTURE_3n5i\| PDB=3n5i \| SCENE= }}~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[3n5i]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Sphingosinicella_xenopeptidilytica Sphingosinicella xenopeptidilytica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N5I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3N5I FirstGlance]. <br>
-	===Crystal structure of the precursor (S250A mutant) of the N-terminal beta-aminopeptidase BapA===	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3n2w\|3n2w]], [[3n33\|3n33]]</td></tr>
-		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BapA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=364098 Sphingosinicella xenopeptidilytica])</td></tr>
-	==~~About this Structure~~==	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3n5i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n5i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3n5i RCSB], [http://www.ebi.ac.uk/pdbsum/3n5i PDBsum]</span></td></tr>
-	[[3n5i]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Sphingosinicella_xenopeptidilytica Sphingosinicella xenopeptidilytica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N5I OCA].	+	</table>

	==See Also==		==See Also==
	*[[Aminopeptidase\|Aminopeptidase]]		*[[Aminopeptidase\|Aminopeptidase]]
		+	__TOC__
		+	</StructureSection>
	[[Category: Sphingosinicella xenopeptidilytica]]		[[Category: Sphingosinicella xenopeptidilytica]]
-	[[Category: Geueke, B.]]	+	[[Category: Geueke, B]]
-	[[Category: Gruetter, M G.]]	+	[[Category: Gruetter, M G]]
-	[[Category: Heck, T.]]	+	[[Category: Heck, T]]
-	[[Category: Kohler, H P.]]	+	[[Category: Kohler, H P]]
-	[[Category: Merz, T.]]	+	[[Category: Merz, T]]
	[[Category: Alpha-beta-beta-alpha sandwich]]		[[Category: Alpha-beta-beta-alpha sandwich]]
	[[Category: Beta-aminopeptidase]]		[[Category: Beta-aminopeptidase]]

OCA at 18:48, 20 October 2012

OCA — Sat, 20 Oct 2012 18:48:26 GMT

←Older revision		Revision as of 18:48, 20 October 2012
Line 7:		Line 7:

	==About this Structure==		==About this Structure==
-	[[3n5i]] is a 4 chain structure ~~of [[Aminopeptidase]]~~ with sequence from [http://en.wikipedia.org/wiki/Sphingosinicella_xenopeptidilytica Sphingosinicella xenopeptidilytica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N5I OCA].	+	[[3n5i]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Sphingosinicella_xenopeptidilytica Sphingosinicella xenopeptidilytica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N5I OCA].

	==See Also==		==See Also==

OCA at 05:17, 27 July 2012

OCA — Fri, 27 Jul 2012 05:17:56 GMT

←Older revision		Revision as of 05:17, 27 July 2012
Line 1:		Line 1:
-	[[Image:3n5i.~~jpg~~\|left\|200px]]	+	[[Image:3n5i.png\|left\|200px]]

-	<!--
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	{{STRUCTURE_3n5i\| PDB=3n5i \| SCENE= }}		{{STRUCTURE_3n5i\| PDB=3n5i \| SCENE= }}

Line 13:		Line 7:

	==About this Structure==		==About this Structure==
-	[[3n5i]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Sphingosinicella_xenopeptidilytica Sphingosinicella xenopeptidilytica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N5I OCA].	+	[[3n5i]] is a 4 chain structure of [[Aminopeptidase]] with sequence from [http://en.wikipedia.org/wiki/Sphingosinicella_xenopeptidilytica Sphingosinicella xenopeptidilytica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N5I OCA].
		+
		+	==See Also==
		+	*[[Aminopeptidase\|Aminopeptidase]]
	[[Category: Sphingosinicella xenopeptidilytica]]		[[Category: Sphingosinicella xenopeptidilytica]]
	[[Category: Geueke, B.]]		[[Category: Geueke, B.]]
Line 20:		Line 17:
	[[Category: Kohler, H P.]]		[[Category: Kohler, H P.]]
	[[Category: Merz, T.]]		[[Category: Merz, T.]]
		+	[[Category: Alpha-beta-beta-alpha sandwich]]
		+	[[Category: Beta-aminopeptidase]]
		+	[[Category: Beta-peptide]]
		+	[[Category: Hydrolase]]
		+	[[Category: Ntn hydrolase]]

OCA: Protected "3n5i" [edit=sysop:move=sysop]

OCA — Wed, 08 Jun 2011 05:18:56 GMT

Protected "3n5i" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:18, 8 June 2011