3n5z - Revision history

OCA at 09:09, 6 September 2023

OCA — Wed, 06 Sep 2023 09:09:24 GMT

←Older revision		Revision as of 09:09, 6 September 2023
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	<StructureSection load='3n5z' size='340' side='right'caption='[[3n5z]], [[Resolution\|resolution]] 2.18Å' scene=''>		<StructureSection load='3n5z' size='340' side='right'caption='[[3n5z]], [[Resolution\|resolution]] 2.18Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3n5z]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Buffalo_rat Buffalo rat~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N5Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3N5Z FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3n5z]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N5Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3N5Z FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=H4B:5,6,7,8-TETRAHYDROBIOPTERIN'>H4B</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=XFM:6-(2-{6-[2-(2-AMINO-6-METHYLPYRIDIN-4-YL)ETHYL]PYRIDIN-2-YL}ETHYL)-4-METHYLPYRIDIN-2-AMINE'>XFM</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.18Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3n5p\|3n5p]], [[3n5q\|3n5q]], [[3n5r\|3n5r]], [[3n5s\|3n5s]], [[3n5t\|3n5t]], [[3n5v\|3n5v]], [[3n5w\|3n5w]], [[3n5x\|3n5x]], [[3n5y\|3n5y]], [[3n60\|3n60]], [[3n61\|3n61]], [[3n62\|3n62]], [[3n63\|3n63]], [[3n64\|3n64]], [[3n65\|3n65]], [[3n66\|3n66]], [[3n67\|3n67]], [[3n68\|3n68]], [[3n69\|3n69]], [[3n6a\|3n6a]], [[3n6b\|3n6b]], [[3n6c\|3n6c]], [[3n6d\|3n6d]], [[3n6e\|3n6e]], [[3n6f\|3n6f]], [[3n6g\|3n6g]]</div></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=H4B:5,6,7,8-TETRAHYDROBIOPTERIN'>H4B</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=XFM:6-(2-{6-[2-(2-AMINO-6-METHYLPYRIDIN-4-YL)ETHYL]PYRIDIN-2-YL}ETHYL)-4-METHYLPYRIDIN-2-AMINE'>XFM</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NOS3 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Nitric-oxide_synthase_(NADPH_dependent) Nitric-oxide synthase (NADPH dependent)], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.13.39 1.14.13.39] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3n5z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n5z OCA], [https://pdbe.org/3n5z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3n5z RCSB], [https://www.ebi.ac.uk/pdbsum/3n5z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3n5z ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3n5z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n5z OCA], [https://pdbe.org/3n5z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3n5z RCSB], [https://www.ebi.ac.uk/pdbsum/3n5z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3n5z ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/NOS1_RAT NOS1_RAT]] Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Inhibitory transmitter for non-adrenergic and non-cholinergic nerves in the colorectum. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR. Inhibitory transmitter for non-adrenergic and non-cholinergic nerves in the colorectum.	+	[https://www.uniprot.org/uniprot/NOS1_RAT NOS1_RAT] Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Inhibitory transmitter for non-adrenergic and non-cholinergic nerves in the colorectum. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR. Inhibitory transmitter for non-adrenergic and non-cholinergic nerves in the colorectum.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 28:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Buffalo rat]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Delker, S L~~]]	+	[[Category: Rattus norvegicus]]
-	[[Category: ~~Li, H~~]]	+	[[Category: Delker SL]]
-	[[Category: ~~Poulos, T L~~]]	+	[[Category: Li H]]
-	[[Category: ~~Heme enzyme]]~~	+	[[Category: Poulos TL]]
-	~~[[Category: Nitric oxide synthase]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Oxidoreductase-oxidoreductase inhibitor complex]]~~	+
-	~~[[Category: Substrate inhibitor]]~~	+
-	~~[[Category: Zn bindingss'~~]]	+

OCA at 06:55, 12 May 2022

OCA — Thu, 12 May 2022 06:55:52 GMT

←Older revision		Revision as of 06:55, 12 May 2022
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	<StructureSection load='3n5z' size='340' side='right'caption='[[3n5z]], [[Resolution\|resolution]] 2.18Å' scene=''>		<StructureSection load='3n5z' size='340' side='right'caption='[[3n5z]], [[Resolution\|resolution]] 2.18Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3n5z]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N5Z OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3N5Z FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3n5z]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N5Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3N5Z FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=H4B:5,6,7,8-TETRAHYDROBIOPTERIN'>H4B</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=XFM:6-(2-{6-[2-(2-AMINO-6-METHYLPYRIDIN-4-YL)ETHYL]PYRIDIN-2-YL}ETHYL)-4-METHYLPYRIDIN-2-AMINE'>XFM</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=H4B:5,6,7,8-TETRAHYDROBIOPTERIN'>H4B</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=XFM:6-(2-{6-[2-(2-AMINO-6-METHYLPYRIDIN-4-YL)ETHYL]PYRIDIN-2-YL}ETHYL)-4-METHYLPYRIDIN-2-AMINE'>XFM</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3n5p\|3n5p]], [[3n5q\|3n5q]], [[3n5r\|3n5r]], [[3n5s\|3n5s]], [[3n5t\|3n5t]], [[3n5v\|3n5v]], [[3n5w\|3n5w]], [[3n5x\|3n5x]], [[3n5y\|3n5y]], [[3n60\|3n60]], [[3n61\|3n61]], [[3n62\|3n62]], [[3n63\|3n63]], [[3n64\|3n64]], [[3n65\|3n65]], [[3n66\|3n66]], [[3n67\|3n67]], [[3n68\|3n68]], [[3n69\|3n69]], [[3n6a\|3n6a]], [[3n6b\|3n6b]], [[3n6c\|3n6c]], [[3n6d\|3n6d]], [[3n6e\|3n6e]], [[3n6f\|3n6f]], [[3n6g\|3n6g]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3n5p\|3n5p]], [[3n5q\|3n5q]], [[3n5r\|3n5r]], [[3n5s\|3n5s]], [[3n5t\|3n5t]], [[3n5v\|3n5v]], [[3n5w\|3n5w]], [[3n5x\|3n5x]], [[3n5y\|3n5y]], [[3n60\|3n60]], [[3n61\|3n61]], [[3n62\|3n62]], [[3n63\|3n63]], [[3n64\|3n64]], [[3n65\|3n65]], [[3n66\|3n66]], [[3n67\|3n67]], [[3n68\|3n68]], [[3n69\|3n69]], [[3n6a\|3n6a]], [[3n6b\|3n6b]], [[3n6c\|3n6c]], [[3n6d\|3n6d]], [[3n6e\|3n6e]], [[3n6f\|3n6f]], [[3n6g\|3n6g]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NOS3 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NOS3 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Nitric-oxide_synthase_(NADPH_dependent) Nitric-oxide synthase (NADPH dependent)], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.13.39 1.14.13.39] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Nitric-oxide_synthase_(NADPH_dependent) Nitric-oxide synthase (NADPH dependent)], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.13.39 1.14.13.39] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3n5z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n5z OCA], [~~http~~://pdbe.org/3n5z PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3n5z RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3n5z PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3n5z ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3n5z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n5z OCA], [https://pdbe.org/3n5z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3n5z RCSB], [https://www.ebi.ac.uk/pdbsum/3n5z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3n5z ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/NOS1_RAT NOS1_RAT]] Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Inhibitory transmitter for non-adrenergic and non-cholinergic nerves in the colorectum. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR. Inhibitory transmitter for non-adrenergic and non-cholinergic nerves in the colorectum.	+	[[https://www.uniprot.org/uniprot/NOS1_RAT NOS1_RAT]] Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Inhibitory transmitter for non-adrenergic and non-cholinergic nerves in the colorectum. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR. Inhibitory transmitter for non-adrenergic and non-cholinergic nerves in the colorectum.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:

	==See Also==		==See Also==
-	*[[Nitric Oxide Synthase\|Nitric Oxide Synthase]]	+	*[[Nitric Oxide Synthase 3D structures\|Nitric Oxide Synthase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:24, 17 July 2019

OCA — Wed, 17 Jul 2019 11:24:24 GMT

←Older revision		Revision as of 11:24, 17 July 2019
Line 1:		Line 1:

	==Structure of neuronal nitric oxide synthase heme domain in complex with 4-(2-(6-(2-(6-amino-4-methylpyridin-2-yl)ethyl)pyridin-2-yl)ethyl)-6-methylpyridin-2-amine==		==Structure of neuronal nitric oxide synthase heme domain in complex with 4-(2-(6-(2-(6-amino-4-methylpyridin-2-yl)ethyl)pyridin-2-yl)ethyl)-6-methylpyridin-2-amine==
-	<StructureSection load='3n5z' size='340' side='right' caption='[[3n5z]], [[Resolution\|resolution]] 2.18Å' scene=''>	+	<StructureSection load='3n5z' size='340' side='right'caption='[[3n5z]], [[Resolution\|resolution]] 2.18Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3n5z]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N5Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3N5Z FirstGlance]. <br>		<table><tr><td colspan='2'>[[3n5z]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N5Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3N5Z FirstGlance]. <br>
Line 21:		Line 21:
	</div>		</div>
	<div class="pdbe-citations 3n5z" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3n5z" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Nitric Oxide Synthase\|Nitric Oxide Synthase]]
	== References ==		== References ==
	<references/>		<references/>
Line 26:		Line 29:
	</StructureSection>		</StructureSection>
	[[Category: Buffalo rat]]		[[Category: Buffalo rat]]
		+	[[Category: Large Structures]]
	[[Category: Delker, S L]]		[[Category: Delker, S L]]
	[[Category: Li, H]]		[[Category: Li, H]]
Line 34:		Line 38:
	[[Category: Oxidoreductase-oxidoreductase inhibitor complex]]		[[Category: Oxidoreductase-oxidoreductase inhibitor complex]]
	[[Category: Substrate inhibitor]]		[[Category: Substrate inhibitor]]
-	[[Category: Zn ~~bindings~~']]	+	[[Category: Zn bindingss']]

OCA at 08:24, 8 November 2017

OCA — Wed, 08 Nov 2017 08:24:23 GMT

←Older revision		Revision as of 08:24, 8 November 2017
Line 21:		Line 21:
	</div>		</div>
	<div class="pdbe-citations 3n5z" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3n5z" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Nitric Oxide Synthase\|Nitric Oxide Synthase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 21:48, 5 August 2016

OCA — Fri, 05 Aug 2016 21:48:05 GMT

←Older revision		Revision as of 21:48, 5 August 2016
Line 1:		Line 1:
		+
	==Structure of neuronal nitric oxide synthase heme domain in complex with 4-(2-(6-(2-(6-amino-4-methylpyridin-2-yl)ethyl)pyridin-2-yl)ethyl)-6-methylpyridin-2-amine==		==Structure of neuronal nitric oxide synthase heme domain in complex with 4-(2-(6-(2-(6-amino-4-methylpyridin-2-yl)ethyl)pyridin-2-yl)ethyl)-6-methylpyridin-2-amine==
	<StructureSection load='3n5z' size='340' side='right' caption='[[3n5z]], [[Resolution\|resolution]] 2.18Å' scene=''>		<StructureSection load='3n5z' size='340' side='right' caption='[[3n5z]], [[Resolution\|resolution]] 2.18Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3n5z]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Rattus_norvegicus Rattus norvegicus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N5Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3N5Z FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3n5z]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N5Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3N5Z FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=H4B:5,6,7,8-TETRAHYDROBIOPTERIN'>H4B</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=XFM:6-(2-{6-[2-(2-AMINO-6-METHYLPYRIDIN-4-YL)ETHYL]PYRIDIN-2-YL}ETHYL)-4-METHYLPYRIDIN-2-AMINE'>XFM</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=H4B:5,6,7,8-TETRAHYDROBIOPTERIN'>H4B</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=XFM:6-(2-{6-[2-(2-AMINO-6-METHYLPYRIDIN-4-YL)ETHYL]PYRIDIN-2-YL}ETHYL)-4-METHYLPYRIDIN-2-AMINE'>XFM</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3n5p\|3n5p]], [[3n5q\|3n5q]], [[3n5r\|3n5r]], [[3n5s\|3n5s]], [[3n5t\|3n5t]], [[3n5v\|3n5v]], [[3n5w\|3n5w]], [[3n5x\|3n5x]], [[3n5y\|3n5y]], [[3n60\|3n60]], [[3n61\|3n61]], [[3n62\|3n62]], [[3n63\|3n63]], [[3n64\|3n64]], [[3n65\|3n65]], [[3n66\|3n66]], [[3n67\|3n67]], [[3n68\|3n68]], [[3n69\|3n69]], [[3n6a\|3n6a]], [[3n6b\|3n6b]], [[3n6c\|3n6c]], [[3n6d\|3n6d]], [[3n6e\|3n6e]], [[3n6f\|3n6f]], [[3n6g\|3n6g]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3n5p\|3n5p]], [[3n5q\|3n5q]], [[3n5r\|3n5r]], [[3n5s\|3n5s]], [[3n5t\|3n5t]], [[3n5v\|3n5v]], [[3n5w\|3n5w]], [[3n5x\|3n5x]], [[3n5y\|3n5y]], [[3n60\|3n60]], [[3n61\|3n61]], [[3n62\|3n62]], [[3n63\|3n63]], [[3n64\|3n64]], [[3n65\|3n65]], [[3n66\|3n66]], [[3n67\|3n67]], [[3n68\|3n68]], [[3n69\|3n69]], [[3n6a\|3n6a]], [[3n6b\|3n6b]], [[3n6c\|3n6c]], [[3n6d\|3n6d]], [[3n6e\|3n6e]], [[3n6f\|3n6f]], [[3n6g\|3n6g]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NOS3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 ~~Rattus norvegicus~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NOS3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitric-~~oxide_synthase~~ Nitric-oxide synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.13.39 1.14.13.39] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitric-oxide_synthase_(NADPH_dependent) Nitric-oxide synthase (NADPH dependent)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.13.39 1.14.13.39] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3n5z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n5z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3n5z RCSB], [http://www.ebi.ac.uk/pdbsum/3n5z PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3n5z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n5z OCA], [http://pdbe.org/3n5z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3n5z RCSB], [http://www.ebi.ac.uk/pdbsum/3n5z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3n5z ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3n5z" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 26:		Line 28:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Nitric-oxide synthase]]~~	+	[[Category: Buffalo rat]]
-	~~[[Category: Rattus norvegicus~~]]	+
	[[Category: Delker, S L]]		[[Category: Delker, S L]]
	[[Category: Li, H]]		[[Category: Li, H]]

OCA at 22:11, 25 December 2014

OCA — Thu, 25 Dec 2014 22:11:27 GMT

←Older revision		Revision as of 22:11, 25 December 2014
Line 9:		Line 9:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3n5z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n5z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3n5z RCSB], [http://www.ebi.ac.uk/pdbsum/3n5z PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3n5z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n5z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3n5z RCSB], [http://www.ebi.ac.uk/pdbsum/3n5z PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/NOS1_RAT NOS1_RAT]] Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Inhibitory transmitter for non-adrenergic and non-cholinergic nerves in the colorectum. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR. Inhibitory transmitter for non-adrenergic and non-cholinergic nerves in the colorectum.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 09:37, 9 December 2014

OCA — Tue, 09 Dec 2014 09:37:18 GMT

←Older revision		Revision as of 09:37, 9 December 2014
Line 1:		Line 1:
-	[[~~Image:~~3n5z.~~png~~\|~~left~~\|~~200px~~]]	+	==Structure of neuronal nitric oxide synthase heme domain in complex with 4-(2-(6-(2-(6-amino-4-methylpyridin-2-yl)ethyl)pyridin-2-yl)ethyl)-6-methylpyridin-2-amine==
		+	<StructureSection load='3n5z' size='340' side='right' caption='[[3n5z]], [[Resolution\|resolution]] 2.18Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3n5z]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N5Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3N5Z FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=H4B:5,6,7,8-TETRAHYDROBIOPTERIN'>H4B</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=XFM:6-(2-{6-[2-(2-AMINO-6-METHYLPYRIDIN-4-YL)ETHYL]PYRIDIN-2-YL}ETHYL)-4-METHYLPYRIDIN-2-AMINE'>XFM</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3n5p\|3n5p]], [[3n5q\|3n5q]], [[3n5r\|3n5r]], [[3n5s\|3n5s]], [[3n5t\|3n5t]], [[3n5v\|3n5v]], [[3n5w\|3n5w]], [[3n5x\|3n5x]], [[3n5y\|3n5y]], [[3n60\|3n60]], [[3n61\|3n61]], [[3n62\|3n62]], [[3n63\|3n63]], [[3n64\|3n64]], [[3n65\|3n65]], [[3n66\|3n66]], [[3n67\|3n67]], [[3n68\|3n68]], [[3n69\|3n69]], [[3n6a\|3n6a]], [[3n6b\|3n6b]], [[3n6c\|3n6c]], [[3n6d\|3n6d]], [[3n6e\|3n6e]], [[3n6f\|3n6f]], [[3n6g\|3n6g]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NOS3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Rattus norvegicus])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitric-oxide_synthase Nitric-oxide synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.13.39 1.14.13.39] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3n5z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n5z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3n5z RCSB], [http://www.ebi.ac.uk/pdbsum/3n5z PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In previous studies [Delker, S. L., et al. (2010), J. Am. Chem. Soc. 132, 5437-5442], we determined the crystal structures of neuronal nitric oxide synthase (nNOS) in complex with nNOS-selective chiral pyrrolidine inhibitors, designed to have an aminopyridine group bound over the heme where it can electrostatically interact with the conserved active site Glu residue. However, in addition to the expected binding mode with the (S,S)-cis inhibitors, an unexpected "flipped" orientation was observed for the (R,R)-cis enantiomers. In the flipped mode, the aminopyridine extends out of the active site where it interacts with one heme propionate. This prompted us to design and synthesize symmetric "double-headed" inhibitors with an aminopyridine at each end of a bridging ring structure [Xue, F., Delker, S. L., Li, H., Fang, J., Jamal, J., Martasek, P., Roman, L. J., Poulos, T. L., and Silverman, R. B. Symmetric double-headed aminopyridines, a novel strategy for potent and membrane-permeable inhibitors of neuronal nitric oxide synthase. J. Med. Chem. (submitted for publication)]. One aminopyridine should interact with the active site Glu and the other with the heme propionate. Crystal structures of these double-headed aminopyridine inhibitors in complexes with nNOS show unexpected and significant protein and heme conformational changes induced by inhibitor binding that result in removal of the tetrahydrobiopterin (H(4)B) cofactor and creation of a new Zn(2+) site. These changes are due to binding of a second inhibitor molecule that results in the displacement of H(4)B and the placement of the inhibitor pyridine group in position to serve as a Zn(2+) ligand together with Asp, His, and a chloride ion. Binding of the second inhibitor molecule and generation of the Zn(2+) site do not occur in eNOS. Structural requirements for creation of the new Zn(2+) site in nNOS were analyzed in detail. These observations open the way for the potential design of novel inhibitors selective for nNOS.

-	~~{{STRUCTURE_3n5z\| PDB=3n5z \| SCENE= }}~~	+	Role of zinc in isoform-selective inhibitor binding to neuronal nitric oxide synthase .,Delker SL, Xue F, Li H, Jamal J, Silverman RB, Poulos TL Biochemistry. 2010 Dec 28;49(51):10803-10. Epub 2010 Dec 7. PMID:21138269<ref>PMID:21138269</ref>

-	~~===Structure~~ of ~~neuronal nitric oxide synthase heme domain in complex with 4-(2-(6-(2-(6-amino-4-methylpyridin-2-yl)ethyl)pyridin-2-yl)ethyl)-6-methylpyridin-2-amine===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_21138269}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3n5z]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Rattus_norvegicus Rattus norvegicus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3N5Z OCA].~~	+

	==See Also==		==See Also==
	*[[Nitric Oxide Synthase\|Nitric Oxide Synthase]]		*[[Nitric Oxide Synthase\|Nitric Oxide Synthase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:021138269<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Nitric-oxide synthase]]		[[Category: Nitric-oxide synthase]]
	[[Category: Rattus norvegicus]]		[[Category: Rattus norvegicus]]
-	[[Category: Delker, S L.]]	+	[[Category: Delker, S L]]
-	[[Category: Li, H.]]	+	[[Category: Li, H]]
-	[[Category: Poulos, T L.]]	+	[[Category: Poulos, T L]]
	[[Category: Heme enzyme]]		[[Category: Heme enzyme]]
	[[Category: Nitric oxide synthase]]		[[Category: Nitric oxide synthase]]

OCA at 22:14, 20 October 2012

OCA — Sat, 20 Oct 2012 22:14:32 GMT

←Older revision		Revision as of 22:14, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3n5z]] is a 2 chain structure ~~of [[Nitric Oxide Synthase]]~~ with sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N5Z OCA].	+	[[3n5z]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N5Z OCA].

	==See Also==		==See Also==

OCA at 15:40, 27 July 2012

OCA — Fri, 27 Jul 2012 15:40:21 GMT

←Older revision		Revision as of 15:40, 27 July 2012
Line 1:		Line 1:
-	[[Image:3n5z.~~jpg~~\|left\|200px]]	+	[[Image:3n5z.png\|left\|200px]]

-	<!--
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	{{STRUCTURE_3n5z\| PDB=3n5z \| SCENE= }}		{{STRUCTURE_3n5z\| PDB=3n5z \| SCENE= }}

	===Structure of neuronal nitric oxide synthase heme domain in complex with 4-(2-(6-(2-(6-amino-4-methylpyridin-2-yl)ethyl)pyridin-2-yl)ethyl)-6-methylpyridin-2-amine===		===Structure of neuronal nitric oxide synthase heme domain in complex with 4-(2-(6-(2-(6-amino-4-methylpyridin-2-yl)ethyl)pyridin-2-yl)ethyl)-6-methylpyridin-2-amine===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_21138269}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 21138269 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_21138269}}		{{ABSTRACT_PUBMED_21138269}}

	==About this Structure==		==About this Structure==
-	[[3n5z]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N5Z OCA].	+	[[3n5z]] is a 2 chain structure of [[Nitric Oxide Synthase]] with sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N5Z OCA].
		+
		+	==See Also==
		+	*[[Nitric Oxide Synthase\|Nitric Oxide Synthase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~21138269~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:021138269</ref><references group="xtra"/>
	[[Category: Nitric-oxide synthase]]		[[Category: Nitric-oxide synthase]]
	[[Category: Rattus norvegicus]]		[[Category: Rattus norvegicus]]
Line 28:		Line 20:
	[[Category: Li, H.]]		[[Category: Li, H.]]
	[[Category: Poulos, T L.]]		[[Category: Poulos, T L.]]
		+	[[Category: Heme enzyme]]
		+	[[Category: Nitric oxide synthase]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Oxidoreductase-oxidoreductase inhibitor complex]]
		+	[[Category: Substrate inhibitor]]
		+	[[Category: Zn bindings']]

OCA: Protected "3n5z" [edit=sysop:move=sysop]

OCA — Wed, 09 Feb 2011 06:09:10 GMT

Protected "3n5z" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:09, 9 February 2011