3n7j - Revision history

OCA at 09:10, 6 September 2023

OCA — Wed, 06 Sep 2023 09:10:02 GMT

←Older revision		Revision as of 09:10, 6 September 2023
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	==Crystal structure of botulinum neurotoxin serotype D binding domain==		==Crystal structure of botulinum neurotoxin serotype D binding domain==
-	<StructureSection load='3n7j' size='340' side='right' caption='[[3n7j]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='3n7j' size='340' side='right'caption='[[3n7j]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3n7j]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N7J OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3N7J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3n7j]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N7J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3N7J FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~>[[3n7k\|3n7k]], [[3n7l\|3n7l]], [[3n7m\|3n7m]]</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">botD, Clostridium phage d-16 phi ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=1491 "Bacillus botulinus" van Ermengem 1896])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3n7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n7j OCA], [https://pdbe.org/3n7j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3n7j RCSB], [https://www.ebi.ac.uk/pdbsum/3n7j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3n7j ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3n7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n7j OCA], [~~http~~://pdbe.org/3n7j PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3n7j RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3n7j PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3n7j ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~BXD_CLOBO BXD_CLOBO]~~] Botulinum toxin ~~acts~~ by inhibiting neurotransmitter release. ~~It binds~~ to ~~peripheral neuronal synapses~~, ~~is internalized and moves~~ by ~~retrograde transport~~ up the ~~axon~~ into the ~~spinal cord where it can move between postsynaptic~~ and ~~presynaptic~~ neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that cleaves the '60-Lys-\|-Leu-61' bond of ~~synaptobrevins~~-1 and -2.	+	[https://www.uniprot.org/uniprot/BXD_CBDP BXD_CBDP] Botulinum toxin causes flaccid paralysis by inhibiting neurotransmitter (acetylcholine) release from the presynaptic membranes of nerve terminals of the eukaryotic host skeletal and autonomic nervous system, with frequent heart or respiratory failure (PubMed:8175689, PubMed:16252491). Precursor of botulinum neurotoxin D for which a proteinaceous coreceptor is controversial. In double SV2A/SV2B knockout mice this toxin does not degrade its synaptobrevin target; introducing SV2A, SV2B or SV2C restores target cleavage (PubMed:21483489). Recognition of SV2 by this toxin does not occur via SV2 glycosylation or its large extracellular loop 4 (PubMed:21483489). Another group does not find a convincing interaction with SV2 (PubMed:21632541). Thus a protein receptor for this BoNT serotype has yet to be definitively proven. Recognizes at least 1 complex polysialylated ganglioside found on neural tissue. Electrical stimulation increases uptake of toxin in an ex vivo assay, presumably by transiently exposing a receptor usually found in eukaryotic target synaptic vesicles (PubMed:19650874, PubMed:21483489, PubMed:21632541). Upon synaptic vesicle recycling the toxin is taken up via the endocytic pathway; when the pH of the toxin-containing endosome drops a structural rearrangement occurs so that the N-terminus of the heavy chain (HC) forms pores that allows the light chain (LC) to translocate into the cytosol (By similarity). Once in the cytosol the disulfide bond linking the 2 subunits is reduced and LC cleaves its target protein on synaptic vesicles, preventing their fusion with the cytoplasmic membrane and thus neurotransmitter release (By similarity). Requires complex eukaryotic host polysialogangliosides for full neurotoxicity and for binding to neurons (PubMed:20704566, PubMed:21483489).[UniProtKB:P0DPI0]<ref>PMID:16252491</ref> <ref>PMID:19650874</ref> <ref>PMID:20704566</ref> <ref>PMID:21483489</ref> <ref>PMID:21632541</ref> <ref>PMID:8175689</ref> Has proteolytic activity (PubMed:8175689, PubMed:8197120). After translocation into the eukaryotic host cytosol, inhibits neurotransmitter release by acting as a zinc endopeptidase that cleaves the '61-Lys-\|-Leu-62' bond of synaptobrevin-1 (VAMP1), and the equivalent 'Lys-\|-Leu' sites in VAMP2 and VAMP3 (PubMed:8175689). Cleaves the '49-Lys-\|-Ile-50' bond of A.californica synaptobrevin (AC P35589) (PubMed:8197120). This chain probably has to be partially unfolded to translocate into the eukaryotic host cell cytosol (PubMed:15584922).<ref>PMID:8175689</ref> <ref>PMID:8197120</ref> <ref>PMID:15584922</ref> Responsible for host epithelial cell transcytosis, host nerve cell targeting and translocation of light chain (LC) into eukaryotic host cell cytosol. Composed of 3 subdomains; the translocation domain (TD), and N-terminus and C-terminus of the receptor-binding domain (RBD). The RBD is responsible for the adherence of the toxin to the eukaryotic target cell surface. The N-terminus of the TD wraps an extended belt around the perimeter of the LC, protecting Zn(2+) in the active site; it may also prevent premature LC dissociation from the translocation channel and protect toxin prior to translocation (PubMed:17907800). The TD inserts into synaptic vesicle membrane to allow translocation into the host cytosol (By similarity). The RBD binds eukaryotic host phosphatidylethanolamine, which may serve as toxin receptor (PubMed:16115873). Treatment of synaptosomes with proteinase K does not reduce HC binding, suggesting there is no protein receptor or it is protected from extracellular proteases (PubMed:16115873). HC significantly decreases uptake and toxicity of whole BoNT/D (PubMed:19650874, PubMed:21483489). HC also interferes with uptake of tetanus toxin (PubMed:19650874). Has 2 closely located carbohydrate-binding receptor sites and binds at least 1 GT1b ganglioside (PubMed:20704566). Bind gangliosides in the order GD2 > GT1b > GD1b (PubMed:21632541). Interacts with eukaryotic target protein SV2B (synaptic vesicle glycoprotein 2B) (PubMed:21483489). Expression of SV2A, SV2B or SV2C in mice knocked-out for the SV2 proteins restores entry of BoNT/D and cleavage of VAMP2, suggesting SV2 acts as its receptor (PubMed:21483489). Unlike BoNT/A and BoNT/E, toxin uptake is not mediated by large extracellular loop 4 of SV2 (PubMed:21483489). Another group finds very poor interaction with SV2 proteins, suggesting the possible protein receptor may not have been identified (PubMed:21632541).[UniProtKB:P0DPI0]<ref>PMID:16115873</ref> <ref>PMID:19650874</ref> <ref>PMID:20704566</ref> <ref>PMID:21483489</ref> <ref>PMID:21632541</ref> <ref>PMID:17907800</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	==See Also==		==See Also==
-	*[[Botulinum neurotoxin\|Botulinum neurotoxin]]	+	*[[Botulinum neurotoxin 3D structures\|Botulinum neurotoxin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus botulinus van ermengem 1896~~]]	+	[[Category: Clostridium botulinum]]
-	[[Category: Baldwin~~, M R~~]]	+	[[Category: Large Structures]]
-	[[Category: Barbieri~~, J T~~]]	+	[[Category: Baldwin MR]]
-	[[Category: Fu, Z]]	+	[[Category: Barbieri JT]]
-	[[Category: Karalewitz, A]]	+	[[Category: Fu Z]]
-	[[Category: Kim, J ~~J.P~~]]	+	[[Category: Karalewitz A]]
-	[[Category: Kroken, A]]	+	[[Category: Kim J-JP]]
-	~~[[Category: Botulinum neurotoxin]]~~	+	[[Category: Kroken A]]
-	~~[[Category: Ganglioside binding loop]]~~	+
-	~~[[Category: Hcr/d]]~~	+
-	~~[[Category: Toxin~~]]	+

OCA at 13:46, 16 January 2019

OCA — Wed, 16 Jan 2019 13:46:38 GMT

←Older revision		Revision as of 13:46, 16 January 2019
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n7/3n7j_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n7/3n7j_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 12:23, 11 August 2016

OCA — Thu, 11 Aug 2016 12:23:18 GMT

←Older revision		Revision as of 12:23, 11 August 2016
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		+
	==Crystal structure of botulinum neurotoxin serotype D binding domain==		==Crystal structure of botulinum neurotoxin serotype D binding domain==
	<StructureSection load='3n7j' size='340' side='right' caption='[[3n7j]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='3n7j' size='340' side='right' caption='[[3n7j]], [[Resolution\|resolution]] 2.00Å' scene=''>
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	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3n7k\|3n7k]], [[3n7l\|3n7l]], [[3n7m\|3n7m]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3n7k\|3n7k]], [[3n7l\|3n7l]], [[3n7m\|3n7m]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">botD, Clostridium phage d-16 phi ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1491 "Bacillus botulinus" van Ermengem 1896])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">botD, Clostridium phage d-16 phi ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1491 "Bacillus botulinus" van Ermengem 1896])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3n7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n7j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3n7j RCSB], [http://www.ebi.ac.uk/pdbsum/3n7j PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3n7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n7j OCA], [http://pdbe.org/3n7j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3n7j RCSB], [http://www.ebi.ac.uk/pdbsum/3n7j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3n7j ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3n7j ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3n7j" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Botulinum neurotoxin\|Botulinum neurotoxin]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:06, 28 January 2015

OCA — Wed, 28 Jan 2015 11:06:43 GMT

←Older revision		Revision as of 11:06, 28 January 2015
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	<StructureSection load='3n7j' size='340' side='right' caption='[[3n7j]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='3n7j' size='340' side='right' caption='[[3n7j]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3n7j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Clostridium_botulinum Clostridium botulinum~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N7J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3N7J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3n7j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N7J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3N7J FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3n7k\|3n7k]], [[3n7l\|3n7l]], [[3n7m\|3n7m]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3n7k\|3n7k]], [[3n7l\|3n7l]], [[3n7m\|3n7m]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">botD, Clostridium phage d-16 phi ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1491 ~~Clostridium botulinum~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">botD, Clostridium phage d-16 phi ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1491 "Bacillus botulinus" van Ermengem 1896])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3n7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n7j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3n7j RCSB], [http://www.ebi.ac.uk/pdbsum/3n7j PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3n7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n7j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3n7j RCSB], [http://www.ebi.ac.uk/pdbsum/3n7j PDBsum]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Clostridium botulinum~~]]	+	[[Category: Bacillus botulinus van ermengem 1896]]
	[[Category: Baldwin, M R]]		[[Category: Baldwin, M R]]
	[[Category: Barbieri, J T]]		[[Category: Barbieri, J T]]
	[[Category: Fu, Z]]		[[Category: Fu, Z]]
	[[Category: Karalewitz, A]]		[[Category: Karalewitz, A]]
-	[[Category: Kim, J-J P]]	+	[[Category: Kim, J J.P]]
	[[Category: Kroken, A]]		[[Category: Kroken, A]]
	[[Category: Botulinum neurotoxin]]		[[Category: Botulinum neurotoxin]]

OCA at 10:08, 25 December 2014

OCA — Thu, 25 Dec 2014 10:08:10 GMT

←Older revision		Revision as of 10:08, 25 December 2014
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3n7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n7j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3n7j RCSB], [http://www.ebi.ac.uk/pdbsum/3n7j PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3n7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n7j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3n7j RCSB], [http://www.ebi.ac.uk/pdbsum/3n7j PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/BXD_CLOBO BXD_CLOBO]] Botulinum toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that cleaves the '60-Lys-\|-Leu-61' bond of synaptobrevins-1 and -2.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 16:32, 18 December 2014

OCA — Thu, 18 Dec 2014 16:32:25 GMT

←Older revision		Revision as of 16:32, 18 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3n7j\| PDB=3n7j \| SCENE= }}~~	+	==Crystal structure of botulinum neurotoxin serotype D binding domain==
-	===Crystal structure of botulinum neurotoxin serotype D binding domain===	+	<StructureSection load='3n7j' size='340' side='right' caption='[[3n7j]], [[Resolution\|resolution]] 2.00Å' scene=''>
-	~~{{ABSTRACT_PUBMED_20731382}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3n7j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N7J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3N7J FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3n7k\|3n7k]], [[3n7l\|3n7l]], [[3n7m\|3n7m]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">botD, Clostridium phage d-16 phi ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1491 Clostridium botulinum])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3n7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n7j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3n7j RCSB], [http://www.ebi.ac.uk/pdbsum/3n7j PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n7/3n7j_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The botulinum neurotoxins (BoNTs) are the most potent protein toxins for humans. There are seven serotypes of BoNTs (A-G) based on a lack of cross antiserum neutralization. BoNTs utilize gangliosides as components of the host receptors for binding and entry into neurons. Members of BoNT/C and BoNT/D serotypes include mosaic toxins that are organized in D/C and C/D toxins. One D/C mosaic toxin, BoNT/D-South Africa (BoNT/D-SA), was not fully neutralized by immunization with BoNT serotype C or D, which stimulated this study. Here the crystal structures of the receptor binding domains of BoNT/C, BoNT/D, and BoNT/D-SA are presented. Biochemical and cell binding studies show that BoNT/C and BoNT/D-SA possess unique mechanisms for ganglioside binding. These studies provide new information about how the BoNTs can enter host cells as well as a basis for understanding the immunological diversity of these neurotoxins.

-	~~==About this Structure==~~	+	Identification of a Unique Ganglioside Binding Loop within Botulinum Neurotoxins C and D-SA .,Karalewitz AP, Kroken AR, Fu Z, Baldwin MR, Kim JJ, Barbieri JT Biochemistry. 2010 Aug 23. PMID:20731382<ref>PMID:20731382</ref>
-	~~[[3n7j]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Clostridium_botulinum Clostridium botulinum]~~. ~~Full crystallographic information is available from [http~~:/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3N7J OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:020731382</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Clostridium botulinum]]		[[Category: Clostridium botulinum]]
-	[[Category: Baldwin, M R.]]	+	[[Category: Baldwin, M R]]
-	[[Category: Barbieri, J T.]]	+	[[Category: Barbieri, J T]]
-	[[Category: Fu, Z.]]	+	[[Category: Fu, Z]]
-	[[Category: Karalewitz, A.]]	+	[[Category: Karalewitz, A]]
-	[[Category: Kim, J-J P.]]	+	[[Category: Kim, J-J P]]
-	[[Category: Kroken, A.]]	+	[[Category: Kroken, A]]
	[[Category: Botulinum neurotoxin]]		[[Category: Botulinum neurotoxin]]
	[[Category: Ganglioside binding loop]]		[[Category: Ganglioside binding loop]]
	[[Category: Hcr/d]]		[[Category: Hcr/d]]
	[[Category: Toxin]]		[[Category: Toxin]]

OCA: Protected "3n7j" [edit=sysop:move=sysop]

OCA — Wed, 17 Apr 2013 21:15:24 GMT

Protected "3n7j" [edit=sysop:move=sysop]

←Older revision

Revision as of 21:15, 17 April 2013

OCA at 21:15, 17 April 2013

OCA — Wed, 17 Apr 2013 21:15:22 GMT

←Older revision		Revision as of 21:15, 17 April 2013
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-	{{Seed}}
-	[[Image:3n7j.png\|left\|200px]]
-
-	<!--
-	The line below this paragraph, containing "STRUCTURE_3n7j", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3n7j\| PDB=3n7j \| SCENE= }}		{{STRUCTURE_3n7j\| PDB=3n7j \| SCENE= }}
-
	===Crystal structure of botulinum neurotoxin serotype D binding domain===		===Crystal structure of botulinum neurotoxin serotype D binding domain===
-
-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_20731382}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 20731382 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_20731382}}		{{ABSTRACT_PUBMED_20731382}}

	==About this Structure==		==About this Structure==
-	~~3N7J~~ is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N7J OCA].	+	[[3n7j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N7J OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~20731382~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:020731382</ref><references group="xtra"/><references/>
	[[Category: Clostridium botulinum]]		[[Category: Clostridium botulinum]]
	[[Category: Baldwin, M R.]]		[[Category: Baldwin, M R.]]
Line 35:		Line 19:
	[[Category: Hcr/d]]		[[Category: Hcr/d]]
	[[Category: Toxin]]		[[Category: Toxin]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 29 07:46:38 2010''

OCA at 04:37, 29 September 2010

OCA — Wed, 29 Sep 2010 04:37:23 GMT

←Older revision		Revision as of 04:37, 29 September 2010
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	{{Seed}}		{{Seed}}
-	[[Image:3n7j.~~jpg~~\|left\|200px]]	+	[[Image:3n7j.png\|left\|200px]]

	<!--		<!--
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	[[Category: Toxin]]		[[Category: Toxin]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Fri~~ Sep ~~10 13~~:59:45 2010''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 29 07:46:38 2010''

OCA at 09:51, 10 September 2010

OCA — Fri, 10 Sep 2010 09:51:16 GMT

←Older revision		Revision as of 09:51, 10 September 2010
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-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3n7j.jpg\|left\|200px]]

-	The ~~entry 3n7j~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3n7j", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3n7j\| PDB=3n7j \| SCENE= }}

-	~~Authors: Fu, Z., Baldwin, M.R., Karalewitz, A., Kroken, A., Barbieri, J.T., Kim, J-J.P.~~	+	===Crystal structure of botulinum neurotoxin serotype D binding domain===

-	Description: Crystal structure of botulinum neurotoxin serotype D binding domain

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Jun 16 08~~:29:20 2010''	+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_20731382}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 20731382 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_20731382}}
		+
		+	==About this Structure==
		+	3N7J is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N7J OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:20731382</ref><references group="xtra"/>
		+	[[Category: Clostridium botulinum]]
		+	[[Category: Baldwin, M R.]]
		+	[[Category: Barbieri, J T.]]
		+	[[Category: Fu, Z.]]
		+	[[Category: Karalewitz, A.]]
		+	[[Category: Kim, J-J P.]]
		+	[[Category: Kroken, A.]]
		+	[[Category: Botulinum neurotoxin]]
		+	[[Category: Ganglioside binding loop]]
		+	[[Category: Hcr/d]]
		+	[[Category: Toxin]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Sep 10 13:59:45 2010''