3n7z - Revision history

OCA at 17:06, 22 March 2017

OCA — Wed, 22 Mar 2017 17:06:02 GMT

←Older revision		Revision as of 17:06, 22 March 2017
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		+
	==Crystal structure of acetyltransferase from Bacillus anthracis==		==Crystal structure of acetyltransferase from Bacillus anthracis==
	<StructureSection load='3n7z' size='340' side='right' caption='[[3n7z]], [[Resolution\|resolution]] 2.75Å' scene=''>		<StructureSection load='3n7z' size='340' side='right' caption='[[3n7z]], [[Resolution\|resolution]] 2.75Å' scene=''>
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	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BAS2743 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=260799 Bacillus anthracis (strain Sterne)])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BAS2743 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=260799 Bacillus anthracis (strain Sterne)])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3n7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n7z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3n7z RCSB], [http://www.ebi.ac.uk/pdbsum/3n7z PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3n7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n7z OCA], [http://pdbe.org/3n7z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3n7z RCSB], [http://www.ebi.ac.uk/pdbsum/3n7z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3n7z ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3n7z ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3n7z" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:48, 17 June 2015

OCA — Wed, 17 Jun 2015 08:48:06 GMT

←Older revision		Revision as of 08:48, 17 June 2015
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	<StructureSection load='3n7z' size='340' side='right' caption='[[3n7z]], [[Resolution\|resolution]] 2.75Å' scene=''>		<StructureSection load='3n7z' size='340' side='right' caption='[[3n7z]], [[Resolution\|resolution]] 2.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3n7z]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bacillus_anthracis~~ Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N7Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3N7Z FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3n7z]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis_(strain_sterne) Bacillus anthracis (strain sterne)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N7Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3N7Z FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BAS2743 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~1392~~ Bacillus anthracis])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BAS2743 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=260799 Bacillus anthracis (strain Sterne)])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3n7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n7z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3n7z RCSB], [http://www.ebi.ac.uk/pdbsum/3n7z PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3n7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n7z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3n7z RCSB], [http://www.ebi.ac.uk/pdbsum/3n7z PDBsum]</span></td></tr>
	</table>		</table>
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Proteins from the enhanced intracellular survival (Eis) family are versatile acetyltransferases that acetylate amines at multiple positions of several aminoglycosides (AGs). Their upregulation confers drug resistance. Homologues of Eis are present in diverse bacteria, including many pathogens. Eis from Mycobacterium tuberculosis (Eis_Mtb) has been well characterized. In this study, we explored the AG specificity and catalytic efficiency of the Eis family protein from Bacillus anthracis (Eis_Ban). Kinetic analysis of specificity and catalytic efficiency of acetylation of six AGs indicates that Eis_Ban displays significant differences from Eis_Mtb in both substrate binding and catalytic efficiency. The number of acetylated amines was also different for several AGs, indicating a distinct regiospecificity of Eis_Ban. Furthermore, most recently identified inhibitors of Eis_Mtb did not inhibit Eis_Ban, underscoring the differences between these two enzymes. To explain these differences, we determined an Eis_Ban crystal structure. The comparison of the crystal structures of Eis_Ban and Eis_Mtb demonstrates that critical residues lining their respective substrate binding pockets differ substantially, explaining their distinct specificities. Our results suggest that acetyltransferases of the Eis family evolved divergently to garner distinct specificities while conserving catalytic efficiency, possibly to counter distinct chemical challenges. The unique specificity features of these enzymes can be utilized as tools for developing AGs with novel modifications and help guide specific AG treatments to avoid Eis-mediated resistance.
		+
		+	Biochemical and Structural Analysis of an Eis Family Aminoglycoside Acetyltransferase from Bacillus anthracis.,Green KD, Biswas T, Chang C, Wu R, Chen W, Janes BK, Chalupska D, Gornicki P, Hanna PC, Tsodikov OV, Joachimiak A, Garneau-Tsodikova S Biochemistry. 2015 May 26;54(20):3197-206. doi: 10.1021/acs.biochem.5b00244. Epub, 2015 May 12. PMID:25928210<ref>PMID:25928210</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillus anthracis]]
	[[Category: Chang, C]]		[[Category: Chang, C]]
	[[Category: Gornicki, P]]		[[Category: Gornicki, P]]

OCA at 16:41, 18 December 2014

OCA — Thu, 18 Dec 2014 16:41:05 GMT

←Older revision		Revision as of 16:41, 18 December 2014
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-	~~{{STRUCTURE_3n7z\| PDB=3n7z \| SCENE= }}~~	+	==Crystal structure of acetyltransferase from Bacillus anthracis==
-	===Crystal structure of acetyltransferase from Bacillus anthracis===	+	<StructureSection load='3n7z' size='340' side='right' caption='[[3n7z]], [[Resolution\|resolution]] 2.75Å' scene=''>
-		+	== Structural highlights ==
-	==~~About this Structure~~==	+	<table><tr><td colspan='2'>[[3n7z]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N7Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3N7Z FirstGlance]. <br>
-	[[3n7z]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N7Z OCA].	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BAS2743 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1392 Bacillus anthracis])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3n7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n7z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3n7z RCSB], [http://www.ebi.ac.uk/pdbsum/3n7z PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n7/3n7z_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	__TOC__
		+	</StructureSection>
	[[Category: Bacillus anthracis]]		[[Category: Bacillus anthracis]]
-	[[Category: Chang, C.]]	+	[[Category: Chang, C]]
-	[[Category: Gornicki, P.]]	+	[[Category: Gornicki, P]]
-	[[Category: Joachimiak, A~~.]]~~	+	[[Category: Joachimiak, A]]
-	~~[[Category: MCSG, Midwest Center for Structural Genomics.]]~~	+
-	~~[[Category: Wu, R.]]~~	+
-	~~[[Category: Zhang, R.]]~~	+
-	~~[[Category: Mcsg]]~~	+
-	~~[[Category: Midwest center for structural genomic]]~~	+
-	~~[[Category: Protein structure initiative]]~~	+
-	~~[[Category: Psi2~~]]	+
	[[Category: Structural genomic]]		[[Category: Structural genomic]]
		+	[[Category: Wu, R]]
		+	[[Category: Zhang, R]]
		+	[[Category: Mcsg]]
		+	[[Category: PSI, Protein structure initiative]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA: Protected "3n7z" [edit=sysop:move=sysop]

OCA — Wed, 17 Apr 2013 20:52:22 GMT

Protected "3n7z" [edit=sysop:move=sysop]

←Older revision

Revision as of 20:52, 17 April 2013

OCA at 20:52, 17 April 2013

OCA — Wed, 17 Apr 2013 20:52:19 GMT

←Older revision		Revision as of 20:52, 17 April 2013
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-	{{Seed}}
-	[[Image:3n7z.jpg\|left\|200px]]
-
-	<!--
-	The line below this paragraph, containing "STRUCTURE_3n7z", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3n7z\| PDB=3n7z \| SCENE= }}		{{STRUCTURE_3n7z\| PDB=3n7z \| SCENE= }}
-
	===Crystal structure of acetyltransferase from Bacillus anthracis===		===Crystal structure of acetyltransferase from Bacillus anthracis===
-

	==About this Structure==		==About this Structure==
-	~~3N7Z~~ is a 6 ~~chains~~ structure with ~~sequences~~ from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N7Z OCA].	+	[[3n7z]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N7Z OCA].
	[[Category: Bacillus anthracis]]		[[Category: Bacillus anthracis]]
	[[Category: Chang, C.]]		[[Category: Chang, C.]]
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	[[Category: Structural genomic]]		[[Category: Structural genomic]]
	[[Category: Transferase]]		[[Category: Transferase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 16 08:44:24 2010''

OCA at 04:39, 16 June 2010

OCA — Wed, 16 Jun 2010 04:39:24 GMT

←Older revision		Revision as of 04:39, 16 June 2010
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-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3n7z.jpg\|left\|200px]]

-	The ~~entry 3n7z~~ is ~~ON HOLD~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3n7z", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3n7z\| PDB=3n7z \| SCENE= }}

-	~~Authors: Chang, C., Wu, R., Gornicki, P., Zhang, R., Joachimiak, A., Midwest Center for Structural Genomics (MCSG)~~	+	===Crystal structure of acetyltransferase from Bacillus anthracis===

-	Description: Crystal structure of acetyltransferase from Bacillus anthracis

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Thu~~ Jun ~~10 10~~:58:27 2010''	+	==About this Structure==
		+	3N7Z is a 6 chains structure with sequences from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N7Z OCA].
		+	[[Category: Bacillus anthracis]]
		+	[[Category: Chang, C.]]
		+	[[Category: Gornicki, P.]]
		+	[[Category: Joachimiak, A.]]
		+	[[Category: MCSG, Midwest Center for Structural Genomics.]]
		+	[[Category: Wu, R.]]
		+	[[Category: Zhang, R.]]
		+	[[Category: Mcsg]]
		+	[[Category: Midwest center for structural genomic]]
		+	[[Category: Protein structure initiative]]
		+	[[Category: Psi2]]
		+	[[Category: Structural genomic]]
		+	[[Category: Transferase]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 16 08:44:24 2010''

OCA at 06:53, 10 June 2010

OCA — Thu, 10 Jun 2010 06:53:40 GMT

←Older revision		Revision as of 06:53, 10 June 2010
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	The entry 3n7z is ON HOLD		The entry 3n7z is ON HOLD

-	Authors: Chang, ~~Changsoo~~, Wu, R., Gornicki, P., Zhang, R., Joachimiak, ~~Andrzej~~	+	Authors: Chang, C., Wu, R., Gornicki, P., Zhang, R., Joachimiak, A., Midwest Center for Structural Genomics (MCSG)

	Description: Crystal structure of acetyltransferase from Bacillus anthracis		Description: Crystal structure of acetyltransferase from Bacillus anthracis

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed~~ Jun ~~2 08~~:29:55 2010''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 10 10:58:27 2010''

OCA: New page: '''Unreleased structure''' The entry 3n7z is ON HOLD Authors: Chang, Changsoo, Wu, R., Gornicki, P., Zhang, R., Joachimiak, Andrzej Description: Crystal structure of acetyltransferase ...

OCA — Wed, 02 Jun 2010 04:25:27 GMT

New page: '''Unreleased structure''' The entry 3n7z is ON HOLD Authors: Chang, Changsoo, Wu, R., Gornicki, P., Zhang, R., Joachimiak, Andrzej Description: Crystal structure of acetyltransferase ...

New page

'''Unreleased structure'''

The entry 3n7z is ON HOLD

Authors: Chang, Changsoo, Wu, R., Gornicki, P., Zhang, R., Joachimiak, Andrzej

Description: Crystal structure of acetyltransferase from Bacillus anthracis

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 2 08:29:55 2010''