3n9k - Revision history

OCA at 16:44, 1 November 2023

OCA — Wed, 01 Nov 2023 16:44:16 GMT

←Older revision		Revision as of 16:44, 1 November 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3n9k]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_albicans Candida albicans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N9K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3N9K FirstGlance]. <br>		<table><tr><td colspan='2'>[[3n9k]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_albicans Candida albicans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N9K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3N9K FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PRD_900024:beta-laminaribiose'>PRD_900024</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PRD_900024:beta-laminaribiose'>PRD_900024</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3n9k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n9k OCA], [https://pdbe.org/3n9k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3n9k RCSB], [https://www.ebi.ac.uk/pdbsum/3n9k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3n9k ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3n9k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n9k OCA], [https://pdbe.org/3n9k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3n9k RCSB], [https://www.ebi.ac.uk/pdbsum/3n9k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3n9k ProSAT]</span></td></tr>
	</table>		</table>

OCA at 04:30, 30 September 2022

OCA — Fri, 30 Sep 2022 04:30:43 GMT

←Older revision		Revision as of 04:30, 30 September 2022
Line 1:		Line 1:

	==F229A/E292S Double Mutant of Exo-beta-1,3-glucanase from Candida albicans in Complex with Laminaritriose at 1.7 A==		==F229A/E292S Double Mutant of Exo-beta-1,3-glucanase from Candida albicans in Complex with Laminaritriose at 1.7 A==
-	<StructureSection load='3n9k' size='340' side='right' caption='[[3n9k]], [[Resolution\|resolution]] 1.70Å' scene=''>	+	<StructureSection load='3n9k' size='340' side='right'caption='[[3n9k]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3n9k]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_11006_[[candida_stellatoidea]] Atcc 11006 [[candida stellatoidea]]~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N9K OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3N9K FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3n9k]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_albicans Candida albicans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N9K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3N9K FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PRD_900024:beta-laminaribiose'>PRD_900024</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1cz1\|1cz1]]~~, ~~[[1eqc\|1eqc]], [[2pbo\|2pbo]], [[2pb1\|2pb1]], [[2pf0\|2pf0]], [[2pc8\|2pc8]]~~<~~/td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3n9k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n9k OCA], [https://pdbe.org/3n9k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3n9k RCSB], [https://www.ebi.ac.uk/pdbsum/3n9k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3n9k ProSAT]</span></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b></td><td class="sblockDat">XOG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5476 ATCC 11006 [[Candida stellatoidea]]])</td></tr>~~	+
-	~~<tr id='activity~~'>~~<td class="sblockLbl"><b>Activity:~~</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucan_1,3-beta-glucosidase Glucan 1,3-beta-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.58 3.2.1.58] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3n9k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n9k OCA], [~~http~~://pdbe.org/3n9k PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3n9k RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3n9k PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3n9k ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~EXG_CANAL EXG_CANAL]~~] Beta-glucanases participate in the metabolism of beta-glucan, the main structural component of the cell wall. ~~It could~~ also function biosynthetically as a transglycosylase.	+	[https://www.uniprot.org/uniprot/EXG1_CANAL EXG1_CANAL] Major glucan 1,3-beta-glucosidase required for cell wall integrity. Beta-glucanases participate in the metabolism of beta-glucan, the main structural component of the cell wall. Can also function biosynthetically as a transglycosylase. Functions to deliver glucan from the cell to the extracellular matrix. Does not appear to impact cell wall glucan content of biofilm cells, nor is it necessary for filamentation or biofilm formation. Involved in cell-substrate and cell-cell adhesion. Adhesion to host-cell surfaces is the first critical step during mucosal infection. XOG1 is target of human antimicrobial peptide LL-37 for inhibition of cell adhesion.<ref>PMID:21713010</ref> <ref>PMID:22876186</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	==See Also==		==See Also==
-	*[[Beta-glucosidase\|Beta-glucosidase]]	+	*[[Beta-glucosidase 3D structures\|Beta-glucosidase 3D structures]]
-	*[[Glucanase\|Glucanase]]	+	*[[Glucanase 3D structures\|Glucanase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Glucan 1,3-beta-glucosidase~~]]	+	[[Category: Candida albicans]]
-	[[Category: ~~Cutfield, J F~~]]	+	[[Category: Large Structures]]
-	[[Category: Cutfield~~, S M~~]]	+	[[Category: Cutfield JF]]
-	[[Category: ~~Nakatani, Y~~]]	+	[[Category: Cutfield SM]]
-	[[Category: ~~Aromatic entranceway/clamp]]~~	+	[[Category: Nakatani Y]]
-	~~[[Category: Exoglucanase]]~~	+
-	~~[[Category: Glycoside hydrolase]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Laminaritriose]]~~	+
-	~~[[Category: Protein-carbohydrate interaction]]~~	+
-	~~[[Category: Site-directed mutagenesis~~]]	+

OCA at 13:14, 16 January 2019

OCA — Wed, 16 Jan 2019 13:14:35 GMT

←Older revision		Revision as of 13:14, 16 January 2019
Line 16:		Line 16:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n9/3n9k_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n9/3n9k_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 16:00, 4 August 2016

OCA — Thu, 04 Aug 2016 16:00:54 GMT

←Older revision		Revision as of 16:00, 4 August 2016
Line 1:		Line 1:
		+
	==F229A/E292S Double Mutant of Exo-beta-1,3-glucanase from Candida albicans in Complex with Laminaritriose at 1.7 A==		==F229A/E292S Double Mutant of Exo-beta-1,3-glucanase from Candida albicans in Complex with Laminaritriose at 1.7 A==
	<StructureSection load='3n9k' size='340' side='right' caption='[[3n9k]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='3n9k' size='340' side='right' caption='[[3n9k]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3n9k]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Candida_albicans Candida albicans~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N9K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3N9K FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3n9k]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_11006_[[candida_stellatoidea]] Atcc 11006 [[candida stellatoidea]]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N9K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3N9K FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1cz1\|1cz1]], [[1eqc\|1eqc]], [[2pbo\|2pbo]], [[2pb1\|2pb1]], [[2pf0\|2pf0]], [[2pc8\|2pc8]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1cz1\|1cz1]], [[1eqc\|1eqc]], [[2pbo\|2pbo]], [[2pb1\|2pb1]], [[2pf0\|2pf0]], [[2pc8\|2pc8]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">XOG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5476 Candida ~~albicans~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">XOG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5476 ATCC 11006 [[Candida stellatoidea]]])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucan_1,3-beta-glucosidase Glucan 1,3-beta-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.58 3.2.1.58] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucan_1,3-beta-glucosidase Glucan 1,3-beta-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.58 3.2.1.58] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3n9k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n9k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3n9k RCSB], [http://www.ebi.ac.uk/pdbsum/3n9k PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3n9k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n9k OCA], [http://pdbe.org/3n9k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3n9k RCSB], [http://www.ebi.ac.uk/pdbsum/3n9k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3n9k ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3n9k ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 29:		Line 30:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3n9k" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 37:		Line 39:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Candida albicans]]
	[[Category: Glucan 1,3-beta-glucosidase]]		[[Category: Glucan 1,3-beta-glucosidase]]
	[[Category: Cutfield, J F]]		[[Category: Cutfield, J F]]

OCA at 10:49, 25 December 2014

OCA — Thu, 25 Dec 2014 10:49:55 GMT

←Older revision		Revision as of 10:49, 25 December 2014
Line 9:		Line 9:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3n9k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n9k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3n9k RCSB], [http://www.ebi.ac.uk/pdbsum/3n9k PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3n9k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n9k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3n9k RCSB], [http://www.ebi.ac.uk/pdbsum/3n9k PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/EXG_CANAL EXG_CANAL]] Beta-glucanases participate in the metabolism of beta-glucan, the main structural component of the cell wall. It could also function biosynthetically as a transglycosylase.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 09:08, 9 December 2014

OCA — Tue, 09 Dec 2014 09:08:35 GMT

←Older revision		Revision as of 09:08, 9 December 2014
Line 1:		Line 1:
-	[[~~Image:~~3n9k.~~png~~\|~~left~~\|200px]]	+	==F229A/E292S Double Mutant of Exo-beta-1,3-glucanase from Candida albicans in Complex with Laminaritriose at 1.7 A==
		+	<StructureSection load='3n9k' size='340' side='right' caption='[[3n9k]], [[Resolution\|resolution]] 1.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3n9k]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Candida_albicans Candida albicans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N9K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3N9K FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1cz1\|1cz1]], [[1eqc\|1eqc]], [[2pbo\|2pbo]], [[2pb1\|2pb1]], [[2pf0\|2pf0]], [[2pc8\|2pc8]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">XOG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5476 Candida albicans])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucan_1,3-beta-glucosidase Glucan 1,3-beta-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.58 3.2.1.58] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3n9k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n9k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3n9k RCSB], [http://www.ebi.ac.uk/pdbsum/3n9k PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n9/3n9k_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Candida albicans exo-beta-1,3-glucanase (Exg; EC 3.2.1.58) is implicated in cell wall beta-d-glucan remodelling through its glucosyl hydrolase and/or transglucosylase activities. A pair of antiparallel phenylalanyl residues (F144 and F258) flank the entrance to the active site pocket. Various Exg mutants were studied using steady-state kinetics and crystallography aiming to understand the roles played by these residues in positioning the beta-1,3-d-glucan substrate. Mutations at the Phe-Phe entranceway demonstrated the requirement for double-sided CH/pi interactions at the +1 subsite, and the necessity for phenylalanine rather than tyrosine or tryptophan. The Tyr-Tyr double mutations introduced ordered water molecules into the entranceway. A third Phe residue (F229) nearby was evaluated as a possible +2 subsite. The inactive double mutant E292S/F229A complexed with laminaritriose has provided the first picture of substrate binding to Exg and demonstrated how the Phe-Phe arrangement acts as a clamp at the +1 subsite. The terminal sugar at the -1 site showed displacement from the position of a monosaccharide analogue with interchange of water molecules and sugar hydroxyls. An unexpected additional glucose binding site, well removed from the active site, was revealed. This site may enable Exg to associate with the branched glucan structure of the C. albicans cell wall.

-	~~{{STRUCTURE_3n9k\| PDB=3n9k \| SCENE= }}~~	+	Carbohydrate binding sites in Candida albicans exo-beta-1,3-glucanase and the role of the Phe-Phe 'clamp' at the active site entrance.,Patrick WM, Nakatani Y, Cutfield SM, Sharpe ML, Ramsay RJ, Cutfield JF FEBS J. 2010 Sep 10. doi: 10.1111/j.1742-4658.2010.07869.x. PMID:20875088<ref>PMID:20875088</ref>

-	~~===F229A~~/~~E292S Double Mutant of Exo-beta-1~~,~~3-glucanase from Candida albicans in Complex with Laminaritriose at 1.7 A===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_20875088}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3n9k]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Candida_albicans Candida albicans]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3N9K OCA].~~	+

	==See Also==		==See Also==
	*[[Beta-glucosidase\|Beta-glucosidase]]		*[[Beta-glucosidase\|Beta-glucosidase]]
	*[[Glucanase\|Glucanase]]		*[[Glucanase\|Glucanase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:020875088<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Candida albicans]]		[[Category: Candida albicans]]
	[[Category: Glucan 1,3-beta-glucosidase]]		[[Category: Glucan 1,3-beta-glucosidase]]
-	[[Category: Cutfield, J F.]]	+	[[Category: Cutfield, J F]]
-	[[Category: Cutfield, S M.]]	+	[[Category: Cutfield, S M]]
-	[[Category: Nakatani, Y.]]	+	[[Category: Nakatani, Y]]
	[[Category: Aromatic entranceway/clamp]]		[[Category: Aromatic entranceway/clamp]]
	[[Category: Exoglucanase]]		[[Category: Exoglucanase]]

OCA at 15:49, 20 October 2012

OCA — Sat, 20 Oct 2012 15:49:38 GMT

←Older revision		Revision as of 15:49, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3n9k]] is a 1 chain structure ~~of [[Beta-glucosidase]] and [[Glucanase]]~~ with sequence from [http://en.wikipedia.org/wiki/Candida_albicans Candida albicans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N9K OCA].	+	[[3n9k]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Candida_albicans Candida albicans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N9K OCA].

	==See Also==		==See Also==

OCA: Protected "3n9k" [edit=sysop:move=sysop]

OCA — Thu, 26 Jul 2012 19:38:15 GMT

Protected "3n9k" [edit=sysop:move=sysop]

←Older revision

Revision as of 19:38, 26 July 2012

OCA at 19:38, 26 July 2012

OCA — Thu, 26 Jul 2012 19:38:14 GMT

←Older revision		Revision as of 19:38, 26 July 2012
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-	{{Seed}}
	[[Image:3n9k.png\|left\|200px]]		[[Image:3n9k.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3n9k", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3n9k\| PDB=3n9k \| SCENE= }}		{{STRUCTURE_3n9k\| PDB=3n9k \| SCENE= }}

	===F229A/E292S Double Mutant of Exo-beta-1,3-glucanase from Candida albicans in Complex with Laminaritriose at 1.7 A===		===F229A/E292S Double Mutant of Exo-beta-1,3-glucanase from Candida albicans in Complex with Laminaritriose at 1.7 A===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_20875088}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 20875088 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_20875088}}		{{ABSTRACT_PUBMED_20875088}}

	==About this Structure==		==About this Structure==
-	~~3N9K~~ is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Candida_albicans Candida albicans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N9K OCA].	+	[[3n9k]] is a 1 chain structure of [[Beta-glucosidase]] and [[Glucanase]] with sequence from [http://en.wikipedia.org/wiki/Candida_albicans Candida albicans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N9K OCA].
		+
		+	==See Also==
		+	*[[Beta-glucosidase\|Beta-glucosidase]]
		+	*[[Glucanase\|Glucanase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~20875088~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:020875088</ref><references group="xtra"/>
	[[Category: Candida albicans]]		[[Category: Candida albicans]]
	[[Category: Glucan 1,3-beta-glucosidase]]		[[Category: Glucan 1,3-beta-glucosidase]]
Line 36:		Line 28:
	[[Category: Protein-carbohydrate interaction]]		[[Category: Protein-carbohydrate interaction]]
	[[Category: Site-directed mutagenesis]]		[[Category: Site-directed mutagenesis]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct 27 11:35:52 2010''

OCA at 08:25, 27 October 2010

OCA — Wed, 27 Oct 2010 08:25:30 GMT

←Older revision		Revision as of 08:25, 27 October 2010
Line 1:		Line 1:
	{{Seed}}		{{Seed}}
-	[[Image:3n9k.~~jpg~~\|left\|200px]]	+	[[Image:3n9k.png\|left\|200px]]

	<!--		<!--
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	===F229A/E292S Double Mutant of Exo-beta-1,3-glucanase from Candida albicans in Complex with Laminaritriose at 1.7 A===		===F229A/E292S Double Mutant of Exo-beta-1,3-glucanase from Candida albicans in Complex with Laminaritriose at 1.7 A===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_20875088}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 20875088 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_20875088}}

	==About this Structure==		==About this Structure==
	3N9K is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Candida_albicans Candida albicans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N9K OCA].		3N9K is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Candida_albicans Candida albicans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N9K OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:20875088</ref><references group="xtra"/>
	[[Category: Candida albicans]]		[[Category: Candida albicans]]
	[[Category: Glucan 1,3-beta-glucosidase]]		[[Category: Glucan 1,3-beta-glucosidase]]
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	[[Category: Site-directed mutagenesis]]		[[Category: Site-directed mutagenesis]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Sep 15 10~~:50:56 2010''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct 27 11:35:52 2010''