3np1 - Revision history

OCA at 06:48, 9 August 2023

OCA — Wed, 09 Aug 2023 06:48:49 GMT

←Older revision		Revision as of 06:48, 9 August 2023
Line 3:		Line 3:
	<StructureSection load='3np1' size='340' side='right'caption='[[3np1]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='3np1' size='340' side='right'caption='[[3np1]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3np1]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Rhopr Rhopr~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NP1 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3NP1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3np1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhodnius_prolixus Rhodnius prolixus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NP1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NP1 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CYN:CYANIDE+ION'>CYN</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3np1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3np1 OCA], [~~http~~://pdbe.org/3np1 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3np1 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3np1 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3np1 ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CYN:CYANIDE+ION'>CYN</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3np1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3np1 OCA], [https://pdbe.org/3np1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3np1 RCSB], [https://www.ebi.ac.uk/pdbsum/3np1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3np1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/NP1_RHOPR NP1_RHOPR]] Heme-based protein that deliver nitric oxide gas (NO) to the victim while feeding, resulting in vasodilation and inhibition of platelet aggregation. Also bind tightly to histamine, which is released by the host to induce wound healing.	+	[https://www.uniprot.org/uniprot/NP1_RHOPR NP1_RHOPR] Heme-based protein that deliver nitric oxide gas (NO) to the victim while feeding, resulting in vasodilation and inhibition of platelet aggregation. Also bind tightly to histamine, which is released by the host to induce wound healing.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 36:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Rhopr~~]]	+	[[Category: Rhodnius prolixus]]
-	[[Category: Andersen~~, J F~~]]	+	[[Category: Andersen JF]]
-	[[Category: Champagne~~, D E~~]]	+	[[Category: Champagne DE]]
-	[[Category: Montfort~~, W R~~]]	+	[[Category: Montfort WR]]
-	[[Category: Walker~~, F A~~]]	+	[[Category: Walker FA]]
-	[[Category: Weichsel, A]]	+	[[Category: Weichsel A]]
-	~~[[Category: Antihistamine]]~~	+
-	~~[[Category: Cyanide]]~~	+
-	~~[[Category: Ferric heme]]~~	+
-	~~[[Category: Histamine]]~~	+
-	~~[[Category: Lipocalin]]~~	+
-	~~[[Category: Nitric oxide transport]]~~	+
-	~~[[Category: Vasodilator~~]]	+

OCA at 16:55, 29 January 2020

OCA — Wed, 29 Jan 2020 16:55:55 GMT

←Older revision		Revision as of 16:55, 29 January 2020
Line 1:		Line 1:

	==CRYSTAL STRUCTURE OF THE COMPLEX OF NITROPHORIN 1 FROM RHODNIUS PROLIXUS WITH CYANIDE==		==CRYSTAL STRUCTURE OF THE COMPLEX OF NITROPHORIN 1 FROM RHODNIUS PROLIXUS WITH CYANIDE==
-	<StructureSection load='3np1' size='340' side='right' caption='[[3np1]], [[Resolution\|resolution]] 2.30Å' scene=''>	+	<StructureSection load='3np1' size='340' side='right'caption='[[3np1]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3np1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhopr Rhopr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NP1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3NP1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3np1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhopr Rhopr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NP1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3NP1 FirstGlance]. <br>
Line 35:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Rhopr]]		[[Category: Rhopr]]
	[[Category: Andersen, J F]]		[[Category: Andersen, J F]]

OCA at 06:05, 18 April 2018

OCA — Wed, 18 Apr 2018 06:05:41 GMT

←Older revision		Revision as of 06:05, 18 April 2018
Line 13:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/np/3np1_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/np/3np1_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 28:		Line 28:
	</div>		</div>
	<div class="pdbe-citations 3np1" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3np1" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Nitrophorin\|Nitrophorin]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 14:46, 5 August 2016

OCA — Fri, 05 Aug 2016 14:46:54 GMT

←Older revision		Revision as of 14:46, 5 August 2016
Line 1:		Line 1:
		+
	==CRYSTAL STRUCTURE OF THE COMPLEX OF NITROPHORIN 1 FROM RHODNIUS PROLIXUS WITH CYANIDE==		==CRYSTAL STRUCTURE OF THE COMPLEX OF NITROPHORIN 1 FROM RHODNIUS PROLIXUS WITH CYANIDE==
	<StructureSection load='3np1' size='340' side='right' caption='[[3np1]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='3np1' size='340' side='right' caption='[[3np1]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3np1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Rhodnius_prolixus Rhodnius prolixus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NP1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3NP1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3np1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhopr Rhopr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NP1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3NP1 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CYN:CYANIDE+ION'>CYN</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CYN:CYANIDE+ION'>CYN</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3np1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3np1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3np1 RCSB], [http://www.ebi.ac.uk/pdbsum/3np1 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3np1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3np1 OCA], [http://pdbe.org/3np1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3np1 RCSB], [http://www.ebi.ac.uk/pdbsum/3np1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3np1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3np1 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 26:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3np1" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Rhodnius prolixus~~]]	+	[[Category: Rhopr]]
	[[Category: Andersen, J F]]		[[Category: Andersen, J F]]
	[[Category: Champagne, D E]]		[[Category: Champagne, D E]]

OCA at 19:41, 25 December 2014

OCA — Thu, 25 Dec 2014 19:41:16 GMT

←Older revision		Revision as of 19:41, 25 December 2014
Line 6:		Line 6:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3np1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3np1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3np1 RCSB], [http://www.ebi.ac.uk/pdbsum/3np1 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3np1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3np1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3np1 RCSB], [http://www.ebi.ac.uk/pdbsum/3np1 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/NP1_RHOPR NP1_RHOPR]] Heme-based protein that deliver nitric oxide gas (NO) to the victim while feeding, resulting in vasodilation and inhibition of platelet aggregation. Also bind tightly to histamine, which is released by the host to induce wound healing.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 06:53, 19 December 2014

OCA — Fri, 19 Dec 2014 06:53:14 GMT

←Older revision		Revision as of 06:53, 19 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3np1\| PDB=3np1 \| SCENE= }}~~	+	==CRYSTAL STRUCTURE OF THE COMPLEX OF NITROPHORIN 1 FROM RHODNIUS PROLIXUS WITH CYANIDE==
-	===CRYSTAL STRUCTURE OF THE COMPLEX OF NITROPHORIN 1 FROM RHODNIUS PROLIXUS WITH CYANIDE===	+	<StructureSection load='3np1' size='340' side='right' caption='[[3np1]], [[Resolution\|resolution]] 2.30Å' scene=''>
-	~~{{ABSTRACT_PUBMED_9546222}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3np1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhodnius_prolixus Rhodnius prolixus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NP1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3NP1 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CYN:CYANIDE+ION'>CYN</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3np1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3np1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3np1 RCSB], [http://www.ebi.ac.uk/pdbsum/3np1 PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/np/3np1_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The nitrophorins are heme-based proteins from the salivary glands of the blood-sucking insect Rhodnius prolixus that deliver nitric oxide gas (NO) to the victim while feeding, resulting in vasodilation and inhibition of platelet aggregation. The nitrophorins also bind tightly to histamine, which is released by the host to induce wound healing. Here we present three crystal structures of nitrophorin 1 (NP1): bound to cyanide, which binds in a manner similar to NO (2.3 A resolution); bound to histamine (2.0 A resolution); and bound to what appears to be NH3 from the crystallization solution (2.0 A resolution). The NP1 structures reveal heme to be sandwiched between strands of a lipocalin-like beta-barrel, and in an arrangement unlike any other gas-transport protein discovered to date. The heme is six-coordinate with a histidine (His 59) on the proximal side, and ligand in a spacious pocket on the distal side. The structures confirm that NO and histamine compete for the same binding pocket and become buried on binding. The dissociation constant for histamine binding was found to be 19 nM, approximately 100-fold lower than that for NO.

-	~~==Function==~~	+	Crystal structures of a nitric oxide transport protein from a blood-sucking insect.,Weichsel A, Andersen JF, Champagne DE, Walker FA, Montfort WR Nat Struct Biol. 1998 Apr;5(4):304-9. PMID:9546222<ref>PMID:9546222</ref>
-	~~[[http://www.uniprot.org/uniprot/NP1_RHOPR NP1_RHOPR]] Heme-based protein that deliver~~ nitric oxide ~~gas (NO) to the victim while feeding, resulting in vasodilation and inhibition of platelet aggregation~~. ~~Also bind tightly to histamine~~, ~~which is released by the host to induce wound healing~~.	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[3np1]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Rhodnius_prolixus Rhodnius prolixus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3NP1 OCA].~~	+	</div>
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:009546222</ref><ref group="xtra">PMID:019140692</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Rhodnius prolixus]]		[[Category: Rhodnius prolixus]]
-	[[Category: Andersen, J F.]]	+	[[Category: Andersen, J F]]
-	[[Category: Champagne, D E.]]	+	[[Category: Champagne, D E]]
-	[[Category: Montfort, W R.]]	+	[[Category: Montfort, W R]]
-	[[Category: Walker, F A.]]	+	[[Category: Walker, F A]]
-	[[Category: Weichsel, A.]]	+	[[Category: Weichsel, A]]
	[[Category: Antihistamine]]		[[Category: Antihistamine]]
	[[Category: Cyanide]]		[[Category: Cyanide]]

OCA: Protected "3np1" [edit=sysop:move=sysop]

OCA — Wed, 24 Apr 2013 12:05:07 GMT

Protected "3np1" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:05, 24 April 2013

OCA at 12:05, 24 April 2013

OCA — Wed, 24 Apr 2013 12:05:02 GMT

←Older revision		Revision as of 12:05, 24 April 2013
Line 1:		Line 1:
-	{{Seed}}
-	[[Image:3np1.png\|left\|200px]]
-
-	<!--
-	The line below this paragraph, containing "STRUCTURE_3np1", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3np1\| PDB=3np1 \| SCENE= }}		{{STRUCTURE_3np1\| PDB=3np1 \| SCENE= }}
-
	===CRYSTAL STRUCTURE OF THE COMPLEX OF NITROPHORIN 1 FROM RHODNIUS PROLIXUS WITH CYANIDE===		===CRYSTAL STRUCTURE OF THE COMPLEX OF NITROPHORIN 1 FROM RHODNIUS PROLIXUS WITH CYANIDE===
		+	{{ABSTRACT_PUBMED_9546222}}

-		+	==Function==
-	~~<!--~~	+	[[http://www.uniprot.org/uniprot/NP1_RHOPR NP1_RHOPR]] Heme-based protein that deliver nitric oxide gas (NO) to the victim while feeding, resulting in vasodilation and inhibition of platelet aggregation. Also bind tightly to histamine, which is released by the host to induce wound healing.
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_9546222}}, adds the Publication Abstract to the page~~	+
-	~~(as it appears on PubMed at~~ http://www.~~pubmed~~.~~gov~~), ~~where 9546222~~ is the ~~PubMed ID number~~.	+
-	~~-->~~	+
-	~~{{ABSTRACT_PUBMED_9546222}}~~	+

	==About this Structure==		==About this Structure==
-	~~3NP1~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Rhodnius_prolixus Rhodnius prolixus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NP1 OCA].	+	[[3np1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhodnius_prolixus Rhodnius prolixus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NP1 OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~9546222~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:009546222</ref><ref group="xtra">PMID:019140692</ref><references group="xtra"/><references/>
	[[Category: Rhodnius prolixus]]		[[Category: Rhodnius prolixus]]
	[[Category: Andersen, J F.]]		[[Category: Andersen, J F.]]
Line 37:		Line 24:
	[[Category: Nitric oxide transport]]		[[Category: Nitric oxide transport]]
	[[Category: Vasodilator]]		[[Category: Vasodilator]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 04:51:59 2009''

OCA at 02:52, 18 February 2009

OCA — Wed, 18 Feb 2009 02:52:01 GMT

←Older revision		Revision as of 02:52, 18 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	3NP1 is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Rhodnius_prolixus Rhodnius prolixus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NP1 OCA].	+	3NP1 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Rhodnius_prolixus Rhodnius prolixus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NP1 OCA].

	==Reference==		==Reference==
-	~~Crystal structures of a nitric oxide transport protein from a blood-sucking insect., Weichsel A, Andersen JF, Champagne DE, Walker FA, Montfort WR, Nat Struct Biol. 1998 Apr;5(4):304-9.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/9546222 9546222]~~	+	<ref group="xtra">PMID:9546222</ref><references group="xtra"/>
	[[Category: Rhodnius prolixus]]		[[Category: Rhodnius prolixus]]
-	[[Category: Single protein]]
	[[Category: Andersen, J F.]]		[[Category: Andersen, J F.]]
	[[Category: Champagne, D E.]]		[[Category: Champagne, D E.]]
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	[[Category: Vasodilator]]		[[Category: Vasodilator]]

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OCA at 09:57, 3 July 2008

OCA — Thu, 03 Jul 2008 09:57:57 GMT

←Older revision		Revision as of 09:57, 3 July 2008
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-	[[Image:3np1.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:3np1.png\|left\|200px]]

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	{{STRUCTURE_3np1\| PDB=3np1 \| SCENE= }}		{{STRUCTURE_3np1\| PDB=3np1 \| SCENE= }}

-	~~'''~~CRYSTAL STRUCTURE OF THE COMPLEX OF NITROPHORIN 1 FROM RHODNIUS PROLIXUS WITH CYANIDE~~'''~~	+	===CRYSTAL STRUCTURE OF THE COMPLEX OF NITROPHORIN 1 FROM RHODNIUS PROLIXUS WITH CYANIDE===


-	~~==Overview==~~	+	<!--
-	~~The nitrophorins are heme~~-~~based proteins from the salivary glands of the blood~~-~~sucking insect Rhodnius prolixus that deliver nitric oxide gas (NO) to the victim while feeding, resulting in vasodilation and inhibition of platelet aggregation.~~ The ~~nitrophorins also bind tightly to histamine~~, ~~which is released by~~ the ~~host~~ to ~~induce wound healing. Here we present three crystal structures of nitrophorin 1~~ (~~NP1)~~: ~~bound to cyanide, which binds in a manner similar to NO (2~~.~~3 A resolution); bound to histamine (2~~.~~0 A resolution~~)~~; and bound to what appears to be NH3 from the crystallization solution (2.0 A resolution). The NP1 structures reveal heme to be sandwiched between strands of a lipocalin-like beta-barrel~~, ~~and in an arrangement unlike any other gas-transport protein discovered to date. The heme~~ is ~~six-coordinate with a histidine (His 59) on~~ the ~~proximal side, and ligand in a spacious pocket on the distal side~~. ~~The structures confirm that NO and histamine compete for the same binding pocket and become buried on binding. The dissociation constant for histamine binding was found to be 19 nM, approximately 100~~-~~fold lower than that for NO.~~	+	The line below this paragraph, {{ABSTRACT_PUBMED_9546222}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 9546222 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_9546222}}

	==About this Structure==		==About this Structure==
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	[[Category: Nitric oxide transport]]		[[Category: Nitric oxide transport]]
	[[Category: Vasodilator]]		[[Category: Vasodilator]]
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