3nsj - Revision history

OCA at 00:33, 28 December 2023

OCA — Thu, 28 Dec 2023 00:33:49 GMT

←Older revision		Revision as of 00:33, 28 December 2023
Line 3:		Line 3:
	<StructureSection load='3nsj' size='340' side='right'caption='[[3nsj]], [[Resolution\|resolution]] 2.75Å' scene=''>		<StructureSection load='3nsj' size='340' side='right'caption='[[3nsj]], [[Resolution\|resolution]] 2.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3nsj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NSJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NSJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3nsj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NSJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NSJ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.75Å</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Prf1, Pfp ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3nsj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nsj OCA], [https://pdbe.org/3nsj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3nsj RCSB], [https://www.ebi.ac.uk/pdbsum/3nsj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3nsj ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3nsj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nsj OCA], [https://pdbe.org/3nsj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3nsj RCSB], [https://www.ebi.ac.uk/pdbsum/3nsj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3nsj ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/PERF_MOUSE PERF_MOUSE]] Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes.<ref>PMID:3261391</ref> <ref>PMID:8164737</ref> <ref>PMID:7526382</ref> <ref>PMID:19446473</ref> <ref>PMID:21037563</ref>	+	[https://www.uniprot.org/uniprot/PERF_MOUSE PERF_MOUSE] Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes.<ref>PMID:3261391</ref> <ref>PMID:8164737</ref> <ref>PMID:7526382</ref> <ref>PMID:19446473</ref> <ref>PMID:21037563</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lk3 transgenic mice~~]]	+	[[Category: Mus musculus]]
-	[[Category: Caradoc-Davies~~, T T~~]]	+	[[Category: Caradoc-Davies TT]]
-	[[Category: Law~~, R H~~]]	+	[[Category: Law RH]]
-	[[Category: Whisstock~~, J C]]~~	+	[[Category: Whisstock JC]]
-	~~[[Category: Immune system]]~~	+
-	~~[[Category: Pore forming protein~~]]	+

OCA at 07:08, 12 May 2022

OCA — Thu, 12 May 2022 07:08:40 GMT

←Older revision		Revision as of 07:08, 12 May 2022
Line 1:		Line 1:

	==The X-ray crystal structure of lymphocyte perforin==		==The X-ray crystal structure of lymphocyte perforin==
-	<StructureSection load='3nsj' size='340' side='right' caption='[[3nsj]], [[Resolution\|resolution]] 2.75Å' scene=''>	+	<StructureSection load='3nsj' size='340' side='right'caption='[[3nsj]], [[Resolution\|resolution]] 2.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3nsj]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NSJ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3NSJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3nsj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NSJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NSJ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Prf1, Pfp ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Prf1, Pfp ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3nsj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nsj OCA], [~~http~~://pdbe.org/3nsj PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3nsj RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3nsj PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3nsj ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3nsj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nsj OCA], [https://pdbe.org/3nsj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3nsj RCSB], [https://www.ebi.ac.uk/pdbsum/3nsj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3nsj ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/PERF_MOUSE PERF_MOUSE]] Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes.<ref>PMID:3261391</ref> <ref>PMID:8164737</ref> <ref>PMID:7526382</ref> <ref>PMID:19446473</ref> <ref>PMID:21037563</ref>	+	[[https://www.uniprot.org/uniprot/PERF_MOUSE PERF_MOUSE]] Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes.<ref>PMID:3261391</ref> <ref>PMID:8164737</ref> <ref>PMID:7526382</ref> <ref>PMID:19446473</ref> <ref>PMID:21037563</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 21:

	==See Also==		==See Also==
-	*[[Cytolysin\|Cytolysin]]	+	*[[Cytolysin 3D structures\|Cytolysin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Lk3 transgenic mice]]		[[Category: Lk3 transgenic mice]]
	[[Category: Caradoc-Davies, T T]]		[[Category: Caradoc-Davies, T T]]

OCA at 10:09, 30 January 2019

OCA — Wed, 30 Jan 2019 10:09:28 GMT

←Older revision		Revision as of 10:09, 30 January 2019
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 3nsj" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3nsj" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Cytolysin\|Cytolysin]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 23:21, 4 August 2016

OCA — Thu, 04 Aug 2016 23:21:36 GMT

←Older revision		Revision as of 23:21, 4 August 2016
Line 1:		Line 1:
		+
	==The X-ray crystal structure of lymphocyte perforin==		==The X-ray crystal structure of lymphocyte perforin==
	<StructureSection load='3nsj' size='340' side='right' caption='[[3nsj]], [[Resolution\|resolution]] 2.75Å' scene=''>		<StructureSection load='3nsj' size='340' side='right' caption='[[3nsj]], [[Resolution\|resolution]] 2.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3nsj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mus_musculus Mus musculus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NSJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3NSJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3nsj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NSJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3NSJ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Prf1, Pfp ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 ~~Mus musculus~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Prf1, Pfp ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3nsj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nsj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3nsj RCSB], [http://www.ebi.ac.uk/pdbsum/3nsj PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3nsj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nsj OCA], [http://pdbe.org/3nsj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3nsj RCSB], [http://www.ebi.ac.uk/pdbsum/3nsj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3nsj ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3nsj" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Mus musculus~~]]	+	[[Category: Lk3 transgenic mice]]
	[[Category: Caradoc-Davies, T T]]		[[Category: Caradoc-Davies, T T]]
	[[Category: Law, R H]]		[[Category: Law, R H]]

OCA at 20:37, 24 December 2014

OCA — Wed, 24 Dec 2014 20:37:59 GMT

←Older revision		Revision as of 20:37, 24 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3nsj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nsj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3nsj RCSB], [http://www.ebi.ac.uk/pdbsum/3nsj PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3nsj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nsj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3nsj RCSB], [http://www.ebi.ac.uk/pdbsum/3nsj PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PERF_MOUSE PERF_MOUSE]] Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes.<ref>PMID:3261391</ref> <ref>PMID:8164737</ref> <ref>PMID:7526382</ref> <ref>PMID:19446473</ref> <ref>PMID:21037563</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 07:03, 19 December 2014

OCA — Fri, 19 Dec 2014 07:03:49 GMT

←Older revision		Revision as of 07:03, 19 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3nsj~~\| ~~PDB~~=3nsj \| ~~SCENE~~= }}	+	==The X-ray crystal structure of lymphocyte perforin==
-	===The X-ray crystal structure of ~~lymphocyte~~ perforin~~===~~	+	<StructureSection load='3nsj' size='340' side='right' caption='[[3nsj]], [[Resolution\|resolution]] 2.75Å' scene=''>
-	~~{{ABSTRACT_PUBMED_21037563}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3nsj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NSJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3NSJ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Prf1, Pfp ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3nsj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nsj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3nsj RCSB], [http://www.ebi.ac.uk/pdbsum/3nsj PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Natural killer cells and cytotoxic T lymphocytes accomplish the critically important function of killing virus-infected and neoplastic cells. They do this by releasing the pore-forming protein perforin and granzyme proteases from cytoplasmic granules into the cleft formed between the abutting killer and target cell membranes. Perforin, a 67-kilodalton multidomain protein, oligomerizes to form pores that deliver the pro-apoptopic granzymes into the cytosol of the target cell. The importance of perforin is highlighted by the fatal consequences of congenital perforin deficiency, with more than 50 different perforin mutations linked to familial haemophagocytic lymphohistiocytosis (type 2 FHL). Here we elucidate the mechanism of perforin pore formation by determining the X-ray crystal structure of monomeric murine perforin, together with a cryo-electron microscopy reconstruction of the entire perforin pore. Perforin is a thin 'key-shaped' molecule, comprising an amino-terminal membrane attack complex perforin-like (MACPF)/cholesterol dependent cytolysin (CDC) domain followed by an epidermal growth factor (EGF) domain that, together with the extreme carboxy-terminal sequence, forms a central shelf-like structure. A C-terminal C2 domain mediates initial, Ca(2+)-dependent membrane binding. Most unexpectedly, however, electron microscopy reveals that the orientation of the perforin MACPF domain in the pore is inside-out relative to the subunit arrangement in CDCs. These data reveal remarkable flexibility in the mechanism of action of the conserved MACPF/CDC fold and provide new insights into how related immune defence molecules such as complement proteins assemble into pores.

-	~~==Function==~~	+	The structural basis for membrane binding and pore formation by lymphocyte perforin.,Law RH, Lukoyanova N, Voskoboinik I, Caradoc-Davies TT, Baran K, Dunstone MA, D'Angelo ME, Orlova EV, Coulibaly F, Verschoor S, Browne KA, Ciccone A, Kuiper MJ, Bird PI, Trapani JA, Saibil HR, Whisstock JC Nature. 2010 Nov 18;468(7322):447-51. Epub 2010 Oct 31. PMID:21037563<ref>PMID:21037563</ref>
-	~~[[http://www~~.~~uniprot.org/uniprot/PERF_MOUSE PERF_MOUSE]] Plays a key role in secretory granule~~-~~dependent cell death~~, ~~and in defense against virus-infected or neoplastic cells~~. ~~Can insert into the membrane of target cells in its calcium~~-~~bound form, oligomerize and form large pores~~. ~~Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes~~.~~<ref>~~PMID:~~3261391</ref> <ref>PMID:8164737</ref> <ref>PMID:7526382</ref> <ref>PMID:19446473</ref>~~ <ref>PMID:21037563</ref>	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[3nsj]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Mus_musculus Mus musculus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3NSJ OCA].~~	+	</div>
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:021037563</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
-	[[Category: Caradoc-Davies, T T.]]	+	[[Category: Caradoc-Davies, T T]]
-	[[Category: Law, R H.]]	+	[[Category: Law, R H]]
-	[[Category: Whisstock, J C.]]	+	[[Category: Whisstock, J C]]
	[[Category: Immune system]]		[[Category: Immune system]]
	[[Category: Pore forming protein]]		[[Category: Pore forming protein]]

OCA: Protected "3nsj" [edit=sysop:move=sysop]

OCA — Wed, 24 Apr 2013 11:44:07 GMT

Protected "3nsj" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:44, 24 April 2013

OCA at 11:44, 24 April 2013

OCA — Wed, 24 Apr 2013 11:44:01 GMT

←Older revision		Revision as of 11:44, 24 April 2013
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-	{{Seed}}
-	[[Image:3nsj.png\|left\|200px]]
-
-	<!--
-	The line below this paragraph, containing "STRUCTURE_3nsj", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3nsj\| PDB=3nsj \| SCENE= }}		{{STRUCTURE_3nsj\| PDB=3nsj \| SCENE= }}
-
	===The X-ray crystal structure of lymphocyte perforin===		===The X-ray crystal structure of lymphocyte perforin===
		+	{{ABSTRACT_PUBMED_21037563}}

-		+	==Function==
-	~~<!--~~	+	[[http://www.uniprot.org/uniprot/PERF_MOUSE PERF_MOUSE]] Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes.<ref>PMID:3261391</ref> <ref>PMID:8164737</ref> <ref>PMID:7526382</ref> <ref>PMID:19446473</ref> <ref>PMID:21037563</ref>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_21037563}}, adds the Publication Abstract to the page~~	+
-	~~(as it appears on PubMed at~~ http://www.~~pubmed~~.~~gov)~~, ~~where 21037563 is the PubMed ID number~~.	+
-	-->	+
-	~~{{ABSTRACT_PUBMED_21037563}}~~	+

	==About this Structure==		==About this Structure==
-	~~3NSJ~~ is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NSJ OCA].	+	[[3nsj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NSJ OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~21037563~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:021037563</ref><references group="xtra"/><references/>
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Caradoc-Davies, T T.]]		[[Category: Caradoc-Davies, T T.]]
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	[[Category: Immune system]]		[[Category: Immune system]]
	[[Category: Pore forming protein]]		[[Category: Pore forming protein]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 15 08:12:16 2010''

OCA at 04:59, 15 December 2010

OCA — Wed, 15 Dec 2010 04:59:57 GMT

←Older revision		Revision as of 04:59, 15 December 2010
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	{{Seed}}		{{Seed}}
-	[[Image:3nsj.~~jpg~~\|left\|200px]]	+	[[Image:3nsj.png\|left\|200px]]

	<!--		<!--
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	===The X-ray crystal structure of lymphocyte perforin===		===The X-ray crystal structure of lymphocyte perforin===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21037563}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21037563 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21037563}}

	==About this Structure==		==About this Structure==
	3NSJ is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NSJ OCA].		3NSJ is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NSJ OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:21037563</ref><references group="xtra"/>
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Caradoc-Davies, T T.]]		[[Category: Caradoc-Davies, T T.]]
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	[[Category: Pore forming protein]]		[[Category: Pore forming protein]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Nov 3 11~~:11:59 2010''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 15 08:12:16 2010''

OCA at 08:01, 3 November 2010

OCA — Wed, 03 Nov 2010 08:01:21 GMT

←Older revision		Revision as of 08:01, 3 November 2010
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-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3nsj.jpg\|left\|200px]]

-	The ~~entry 3nsj~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3nsj", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3nsj\| PDB=3nsj \| SCENE= }}

-	~~Authors: Law, R.H., Whisstock, J.C., Caradoc~~-~~Davies, T.T.~~	+	===The X-ray crystal structure of lymphocyte perforin===

-	Description: The X-ray crystal structure of lymphocyte perforin

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Oct 13 10~~:29:46 2010''	+	==About this Structure==
		+	3NSJ is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NSJ OCA].
		+	[[Category: Mus musculus]]
		+	[[Category: Caradoc-Davies, T T.]]
		+	[[Category: Law, R H.]]
		+	[[Category: Whisstock, J C.]]
		+	[[Category: Immune system]]
		+	[[Category: Pore forming protein]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Nov 3 11:11:59 2010''