3o02 - Revision history

OCA at 10:02, 14 February 2024

OCA — Wed, 14 Feb 2024 10:02:27 GMT

←Older revision		Revision as of 10:02, 14 February 2024
Line 3:		Line 3:
	<StructureSection load='3o02' size='340' side='right'caption='[[3o02]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='3o02' size='340' side='right'caption='[[3o02]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3o02]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_typhimurium"_loeffler_1892 "bacillus typhimurium" loeffler 1892~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O02 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3O02 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3o02]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O02 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3O02 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=JN3:CHENODEOXYCHOLIC+ACID'>JN3</scene>~~, ~~<scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[3nzz\|3nzz]], [[3o00\|3o00]], [[3o01\|3o01]]~~</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=JN3:CHENODEOXYCHOLIC+ACID'>JN3</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">sipD, sspD, STM2883 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=90371 "Bacillus typhimurium" Loeffler 1892])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3o02 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o02 OCA], [https://pdbe.org/3o02 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3o02 RCSB], [https://www.ebi.ac.uk/pdbsum/3o02 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3o02 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3o02 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o02 OCA], [https://pdbe.org/3o02 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3o02 RCSB], [https://www.ebi.ac.uk/pdbsum/3o02 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3o02 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/SIPD_SALTY SIPD_SALTY]] Required for translocation of effector proteins via the type III secretion system SPI-1, which is essential for an efficient bacterial internalization. Probably acts by modulating the secretion of SipA, SipB, and SipC.<ref>PMID:8522512</ref> <ref>PMID:9194702</ref>	+	[https://www.uniprot.org/uniprot/SIPD_SALTY SIPD_SALTY] Required for translocation of effector proteins via the type III secretion system SPI-1, which is essential for an efficient bacterial internalization. Probably acts by modulating the secretion of SipA, SipB, and SipC.<ref>PMID:8522512</ref> <ref>PMID:9194702</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	The type III secretion system (T3SS) is a protein injection nanomachinery required for virulence by many human pathogenic bacteria including Salmonella and Shigella. An essential component of the T3SS is the tip protein and the Salmonella SipD and the Shigella IpaD tip proteins interact with bile salts, which serve as environmental sensors for these enteric pathogens. SipD and IpaD have long central coiled coils and their N-terminal regions form alpha-helical hairpins and a short helix alpha3 that pack against the coiled coil. Using AutoDock, others have predicted that the bile salt deoxycholate binds IpaD in a cleft formed by the alpha-helical hairpin and its long central coiled coil. NMR chemical shift mapping, however, indicated that the SipD residues most affected by bile salts are located in a disordered region near helix alpha3. Thus, how bile salts interact with SipD and IpaD is unclear. Here, we report the crystal structures of SipD in complex with the bile salts deoxycholate and chenodeoxycholate. Bile salts bind SipD in a region different from what was predicted for IpaD. In SipD, bile salts bind part of helix alpha3 and the C-terminus of the long central coiled coil, towards the C-terminus of the protein. We discuss the biological implication of the differences in how bile salts interact with SipD and IpaD.	+
-		+
-	The crystal structures of the Salmonella type III secretion system tip protein SipD in complex with deoxycholate and chenodeoxycholate.,Chatterjee S, Zhong D, Nordhues BA, Battaile KP, Lovell SW, De Guzman RN Protein Sci. 2010 Oct 28. PMID:21031487<ref>PMID:21031487</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3o02" style="background-color:#fffaf0;"></div~~>	+
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillus typhimurium loeffler 1892]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Battaile~~, K P~~]]	+	[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
-	[[Category: Chatterjee, S]]	+	[[Category: Battaile KP]]
-	[[Category: DeGuzman~~, R N~~]]	+	[[Category: Chatterjee S]]
-	[[Category: Lovell, S]]	+	[[Category: DeGuzman RN]]
-	[[Category: Nordhues~~, B A~~]]	+	[[Category: Lovell S]]
-	[[Category: Zhong, D]]	+	[[Category: Nordhues BA]]
-	~~[[Category: Bacterial needle tip protein]]~~	+	[[Category: Zhong D]]
-	~~[[Category: Cell invasion]]~~	+
-	~~[[Category: Coiled-coil]]~~	+
-	~~[[Category: Prgi]]~~	+
-	~~[[Category: Sipb]]~~	+
-	~~[[Category: Sipc~~]]	+

OCA at 07:12, 12 May 2022

OCA — Thu, 12 May 2022 07:12:40 GMT

←Older revision		Revision as of 07:12, 12 May 2022
Line 1:		Line 1:

	==The Crystal Structure of the Salmonella Type III Secretion System Tip Protein SipD in Complex with Chenodeoxycholate==		==The Crystal Structure of the Salmonella Type III Secretion System Tip Protein SipD in Complex with Chenodeoxycholate==
-	<StructureSection load='3o02' size='340' side='right' caption='[[3o02]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='3o02' size='340' side='right'caption='[[3o02]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3o02]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_typhimurium"_loeffler_1892 "bacillus typhimurium" loeffler 1892]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O02 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3O02 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3o02]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_typhimurium"_loeffler_1892 "bacillus typhimurium" loeffler 1892]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O02 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3O02 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=JN3:CHENODEOXYCHOLIC+ACID'>JN3</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=JN3:CHENODEOXYCHOLIC+ACID'>JN3</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3nzz\|3nzz]], [[3o00\|3o00]], [[3o01\|3o01]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3nzz\|3nzz]], [[3o00\|3o00]], [[3o01\|3o01]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">sipD, sspD, STM2883 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=90371 "Bacillus typhimurium" Loeffler 1892])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">sipD, sspD, STM2883 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=90371 "Bacillus typhimurium" Loeffler 1892])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3o02 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o02 OCA], [~~http~~://pdbe.org/3o02 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3o02 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3o02 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3o02 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3o02 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o02 OCA], [https://pdbe.org/3o02 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3o02 RCSB], [https://www.ebi.ac.uk/pdbsum/3o02 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3o02 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/SIPD_SALTY SIPD_SALTY]] Required for translocation of effector proteins via the type III secretion system SPI-1, which is essential for an efficient bacterial internalization. Probably acts by modulating the secretion of SipA, SipB, and SipC.<ref>PMID:8522512</ref> <ref>PMID:9194702</ref>	+	[[https://www.uniprot.org/uniprot/SIPD_SALTY SIPD_SALTY]] Required for translocation of effector proteins via the type III secretion system SPI-1, which is essential for an efficient bacterial internalization. Probably acts by modulating the secretion of SipA, SipB, and SipC.<ref>PMID:8522512</ref> <ref>PMID:9194702</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 25:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus typhimurium loeffler 1892]]		[[Category: Bacillus typhimurium loeffler 1892]]
		+	[[Category: Large Structures]]
	[[Category: Battaile, K P]]		[[Category: Battaile, K P]]
	[[Category: Chatterjee, S]]		[[Category: Chatterjee, S]]

OCA at 12:53, 5 August 2016

OCA — Fri, 05 Aug 2016 12:53:08 GMT

←Older revision		Revision as of 12:53, 5 August 2016
Line 1:		Line 1:
		+
	==The Crystal Structure of the Salmonella Type III Secretion System Tip Protein SipD in Complex with Chenodeoxycholate==		==The Crystal Structure of the Salmonella Type III Secretion System Tip Protein SipD in Complex with Chenodeoxycholate==
	<StructureSection load='3o02' size='340' side='right' caption='[[3o02]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='3o02' size='340' side='right' caption='[[3o02]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3o02]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Salmonella_enterica Salmonella enterica~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O02 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3O02 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3o02]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_typhimurium"_loeffler_1892 "bacillus typhimurium" loeffler 1892]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O02 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3O02 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=JN3:CHENODEOXYCHOLIC+ACID'>JN3</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=JN3:CHENODEOXYCHOLIC+ACID'>JN3</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3nzz\|3nzz]], [[3o00\|3o00]], [[3o01\|3o01]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3nzz\|3nzz]], [[3o00\|3o00]], [[3o01\|3o01]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">sipD, sspD, STM2883 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~28901 Salmonella enterica~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">sipD, sspD, STM2883 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=90371 "Bacillus typhimurium" Loeffler 1892])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3o02 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o02 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3o02 RCSB], [http://www.ebi.ac.uk/pdbsum/3o02 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3o02 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o02 OCA], [http://pdbe.org/3o02 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3o02 RCSB], [http://www.ebi.ac.uk/pdbsum/3o02 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3o02 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3o02" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Salmonella enterica~~]]	+	[[Category: Bacillus typhimurium loeffler 1892]]
	[[Category: Battaile, K P]]		[[Category: Battaile, K P]]
	[[Category: Chatterjee, S]]		[[Category: Chatterjee, S]]

OCA at 02:37, 25 December 2014

OCA — Thu, 25 Dec 2014 02:37:23 GMT

←Older revision		Revision as of 02:37, 25 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3o02 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o02 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3o02 RCSB], [http://www.ebi.ac.uk/pdbsum/3o02 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3o02 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o02 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3o02 RCSB], [http://www.ebi.ac.uk/pdbsum/3o02 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SIPD_SALTY SIPD_SALTY]] Required for translocation of effector proteins via the type III secretion system SPI-1, which is essential for an efficient bacterial internalization. Probably acts by modulating the secretion of SipA, SipB, and SipC.<ref>PMID:8522512</ref> <ref>PMID:9194702</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 07:24, 19 December 2014

OCA — Fri, 19 Dec 2014 07:24:20 GMT

←Older revision		Revision as of 07:24, 19 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3o02\| PDB=3o02 \| SCENE= }}~~	+	==The Crystal Structure of the Salmonella Type III Secretion System Tip Protein SipD in Complex with Chenodeoxycholate==
-	===The Crystal Structure of the Salmonella Type III Secretion System Tip Protein SipD in Complex with Chenodeoxycholate===	+	<StructureSection load='3o02' size='340' side='right' caption='[[3o02]], [[Resolution\|resolution]] 1.90Å' scene=''>
-	~~{{ABSTRACT_PUBMED_21031487}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3o02]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Salmonella_enterica Salmonella enterica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O02 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3O02 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=JN3:CHENODEOXYCHOLIC+ACID'>JN3</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3nzz\|3nzz]], [[3o00\|3o00]], [[3o01\|3o01]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">sipD, sspD, STM2883 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28901 Salmonella enterica])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3o02 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o02 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3o02 RCSB], [http://www.ebi.ac.uk/pdbsum/3o02 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The type III secretion system (T3SS) is a protein injection nanomachinery required for virulence by many human pathogenic bacteria including Salmonella and Shigella. An essential component of the T3SS is the tip protein and the Salmonella SipD and the Shigella IpaD tip proteins interact with bile salts, which serve as environmental sensors for these enteric pathogens. SipD and IpaD have long central coiled coils and their N-terminal regions form alpha-helical hairpins and a short helix alpha3 that pack against the coiled coil. Using AutoDock, others have predicted that the bile salt deoxycholate binds IpaD in a cleft formed by the alpha-helical hairpin and its long central coiled coil. NMR chemical shift mapping, however, indicated that the SipD residues most affected by bile salts are located in a disordered region near helix alpha3. Thus, how bile salts interact with SipD and IpaD is unclear. Here, we report the crystal structures of SipD in complex with the bile salts deoxycholate and chenodeoxycholate. Bile salts bind SipD in a region different from what was predicted for IpaD. In SipD, bile salts bind part of helix alpha3 and the C-terminus of the long central coiled coil, towards the C-terminus of the protein. We discuss the biological implication of the differences in how bile salts interact with SipD and IpaD.

-	~~==Function==~~	+	The crystal structures of the Salmonella type III secretion system tip protein SipD in complex with deoxycholate and chenodeoxycholate.,Chatterjee S, Zhong D, Nordhues BA, Battaile KP, Lovell SW, De Guzman RN Protein Sci. 2010 Oct 28. PMID:21031487<ref>PMID:21031487</ref>
-	~~[[http://www.uniprot.org/uniprot/SIPD_SALTY SIPD_SALTY]] Required for translocation~~ of ~~effector proteins via~~ the type III secretion system ~~SPI-1, which is essential for an efficient bacterial internalization~~. ~~Probably acts by modulating the secretion of SipA~~, ~~SipB~~, ~~and SipC~~.~~<ref>~~PMID:~~8522512</ref>~~ <ref>PMID:~~9194702~~</ref>	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[3o02]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Salmonella_enterica Salmonella enterica]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3O02 OCA].~~	+	</div>
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:021031487</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Salmonella enterica]]		[[Category: Salmonella enterica]]
-	[[Category: Battaile, K P.]]	+	[[Category: Battaile, K P]]
-	[[Category: Chatterjee, S.]]	+	[[Category: Chatterjee, S]]
-	[[Category: DeGuzman, R N.]]	+	[[Category: DeGuzman, R N]]
-	[[Category: Lovell, S.]]	+	[[Category: Lovell, S]]
-	[[Category: Nordhues, B A.]]	+	[[Category: Nordhues, B A]]
-	[[Category: Zhong, D.]]	+	[[Category: Zhong, D]]
	[[Category: Bacterial needle tip protein]]		[[Category: Bacterial needle tip protein]]
	[[Category: Cell invasion]]		[[Category: Cell invasion]]

OCA at 12:07, 24 April 2013

OCA — Wed, 24 Apr 2013 12:07:20 GMT

←Older revision		Revision as of 12:07, 24 April 2013
Line 1:		Line 1:
-	[[Image:3o02.png\|left\|200px]]
-
-	<!--
-	The line below this paragraph, containing "STRUCTURE_3o02", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3o02\| PDB=3o02 \| SCENE= }}		{{STRUCTURE_3o02\| PDB=3o02 \| SCENE= }}
-
	===The Crystal Structure of the Salmonella Type III Secretion System Tip Protein SipD in Complex with Chenodeoxycholate===		===The Crystal Structure of the Salmonella Type III Secretion System Tip Protein SipD in Complex with Chenodeoxycholate===
		+	{{ABSTRACT_PUBMED_21031487}}

-		+	==Function==
-	~~<!--~~	+	[[http://www.uniprot.org/uniprot/SIPD_SALTY SIPD_SALTY]] Required for translocation of effector proteins via the type III secretion system SPI-1, which is essential for an efficient bacterial internalization. Probably acts by modulating the secretion of SipA, SipB, and SipC.<ref>PMID:8522512</ref> <ref>PMID:9194702</ref>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_21031487}}, adds the Publication Abstract to the page~~	+
-	~~(as it appears on PubMed at~~ http://www.~~pubmed~~.~~gov)~~, ~~where 21031487~~ is the ~~PubMed ID number~~.	+
-	-->	+
-	~~{{ABSTRACT_PUBMED_21031487}}~~	+

	==About this Structure==		==About this Structure==
Line 22:		Line 10:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~21031487~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:021031487</ref><references group="xtra"/><references/>
	[[Category: Salmonella enterica]]		[[Category: Salmonella enterica]]
	[[Category: Battaile, K P.]]		[[Category: Battaile, K P.]]
Line 30:		Line 18:
	[[Category: Nordhues, B A.]]		[[Category: Nordhues, B A.]]
	[[Category: Zhong, D.]]		[[Category: Zhong, D.]]
		+	[[Category: Bacterial needle tip protein]]
		+	[[Category: Cell invasion]]
		+	[[Category: Coiled-coil]]
		+	[[Category: Prgi]]
		+	[[Category: Sipb]]
		+	[[Category: Sipc]]

OCA: Protected "3o02" [edit=sysop:move=sysop]

OCA — Wed, 05 Jan 2011 15:43:55 GMT

Protected "3o02" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:43, 5 January 2011

OCA at 15:43, 5 January 2011

OCA — Wed, 05 Jan 2011 15:43:54 GMT

←Older revision		Revision as of 15:43, 5 January 2011
Line 1:		Line 1:
-	~~{{Seed}}~~	+	[[Image:3o02.png\|left\|200px]]
-	[[Image:3o02.~~jpg~~\|left\|200px]]	+

	<!--		<!--
Line 20:		Line 19:

	==About this Structure==		==About this Structure==
-	~~3O02~~ is a 2 ~~chains~~ structure with ~~sequences~~ from [http://en.wikipedia.org/wiki/Salmonella_enterica Salmonella enterica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O02 OCA].	+	[[3o02]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Salmonella_enterica Salmonella enterica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O02 OCA].

	==Reference==		==Reference==
Line 31:		Line 30:
	[[Category: Nordhues, B A.]]		[[Category: Nordhues, B A.]]
	[[Category: Zhong, D.]]		[[Category: Zhong, D.]]
-	[[Category: Bacterial needle tip protein]]
-	[[Category: Cell invasion]]
-	[[Category: Coiled-coil]]
-	[[Category: Prgi]]
-	[[Category: Sipb]]
-	[[Category: Sipc]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Nov 18 00:51:44 2010''

OCA at 21:40, 17 November 2010

OCA — Wed, 17 Nov 2010 21:40:30 GMT

←Older revision		Revision as of 21:40, 17 November 2010
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3o02.jpg\|left\|200px]]

-	The ~~entry 3o02~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3o02", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3o02\| PDB=3o02 \| SCENE= }}

-	~~Authors: Chatterjee, S., Zhong, D., Nordhues, B.A., Battaile, K.P., Lovell, S., DeGuzman, R.N.~~	+	===The Crystal Structure of the Salmonella Type III Secretion System Tip Protein SipD in Complex with Chenodeoxycholate===

-	Description: The Crystal Structure of the Salmonella Type III Secretion System Tip Protein SipD in Complex with Chenodeoxycholate

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Aug 4 08~~:51:08 2010''	+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21031487}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21031487 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21031487}}
		+
		+	==About this Structure==
		+	3O02 is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Salmonella_enterica Salmonella enterica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O02 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:21031487</ref><references group="xtra"/>
		+	[[Category: Salmonella enterica]]
		+	[[Category: Battaile, K P.]]
		+	[[Category: Chatterjee, S.]]
		+	[[Category: DeGuzman, R N.]]
		+	[[Category: Lovell, S.]]
		+	[[Category: Nordhues, B A.]]
		+	[[Category: Zhong, D.]]
		+	[[Category: Bacterial needle tip protein]]
		+	[[Category: Cell invasion]]
		+	[[Category: Coiled-coil]]
		+	[[Category: Prgi]]
		+	[[Category: Sipb]]
		+	[[Category: Sipc]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Nov 18 00:51:44 2010''

OCA at 04:44, 4 August 2010

OCA — Wed, 04 Aug 2010 04:44:09 GMT

←Older revision		Revision as of 04:44, 4 August 2010
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3o02 is ON HOLD	+	The entry 3o02 is ON HOLD until Paper Publication

-	Authors: Chatterjee, S, Zhong, D, Nordhues, B.A., Battaile, K.P., Lovell, S, DeGuzman, R.N.	+	Authors: Chatterjee, S., Zhong, D., Nordhues, B.A., Battaile, K.P., Lovell, S., DeGuzman, R.N.

	Description: The Crystal Structure of the Salmonella Type III Secretion System Tip Protein SipD in Complex with Chenodeoxycholate		Description: The Crystal Structure of the Salmonella Type III Secretion System Tip Protein SipD in Complex with Chenodeoxycholate

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Jul 28 12~~:27:11 2010''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug 4 08:51:08 2010''