3o3f - Revision history

OCA at 10:03, 14 February 2024

OCA — Wed, 14 Feb 2024 10:03:12 GMT

←Older revision		Revision as of 10:03, 14 February 2024
Line 3:		Line 3:
	<StructureSection load='3o3f' size='340' side='right'caption='[[3o3f]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='3o3f' size='340' side='right'caption='[[3o3f]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3o3f]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_43589 Atcc 43589~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O3F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3O3F FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3o3f]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O3F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3O3F FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max~~-~~height: 3em;'>[[3o3g\|3o3g]]~~, [[~~3o3h~~\|~~3o3h~~]]~~, [[2etj\|2etj]]</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>rnhB, TM_0915 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~2336 ATCC 43589])</td~~><~~/tr>~~	+
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Ribonuclease_H Ribonuclease H], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.4 3.1.26.4]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3o3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o3f OCA], [https://pdbe.org/3o3f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3o3f RCSB], [https://www.ebi.ac.uk/pdbsum/3o3f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3o3f ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3o3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o3f OCA], [https://pdbe.org/3o3f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3o3f RCSB], [https://www.ebi.ac.uk/pdbsum/3o3f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3o3f ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/RNH2_THEMA RNH2_THEMA]] Endonuclease that specifically degrades the RNA of RNA-DNA hybrids (By similarity).	+	[https://www.uniprot.org/uniprot/RNH2_THEMA RNH2_THEMA] Endonuclease that specifically degrades the RNA of RNA-DNA hybrids (By similarity).
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Two classes of RNase H hydrolyze RNA of RNA/DNA hybrids. In contrast to RNase H1 that requires four ribonucleotides for cleavage, RNase H2 can nick duplex DNAs containing a single ribonucleotide, suggesting different in vivo substrates. We report here the crystal structures of a type 2 RNase H in complex with substrates containing a (5')RNA-DNA(3') junction. They revealed a unique mechanism of recognition and substrate-assisted cleavage. A conserved tyrosine residue distorts the nucleic acid at the junction, allowing the substrate to function in catalysis by participating in coordination of the active site metal ion. The biochemical and structural properties of RNase H2 explain the preference of the enzyme for junction substrates and establish the structural and mechanistic differences with RNase H1. Junction recognition is important for the removal of RNA embedded in DNA and may play an important role in DNA replication and repair.	+
-		+
-	Crystal Structures of RNase H2 in Complex with Nucleic Acid Reveal the Mechanism of RNA-DNA Junction Recognition and Cleavage.,Rychlik MP, Chon H, Cerritelli SM, Klimek P, Crouch RJ, Nowotny M Mol Cell. 2010 Nov 24;40(4):658-70. PMID:21095591<ref>PMID:21095591</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3o3f" style="background-color:#fffaf0;"></div>~~	+

	==See Also==		==See Also==
	*[[Ribonuclease 3D structures\|Ribonuclease 3D structures]]		*[[Ribonuclease 3D structures\|Ribonuclease 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 43589]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Ribonuclease H~~]]	+	[[Category: Thermotoga maritima]]
-	[[Category: Cerritelli~~, S M~~]]	+	[[Category: Cerritelli SM]]
-	[[Category: Chon, H]]	+	[[Category: Chon H]]
-	[[Category: Crouch~~, R J~~]]	+	[[Category: Crouch RJ]]
-	[[Category: Klimek, P]]	+	[[Category: Klimek P]]
-	[[Category: Nowotny, M]]	+	[[Category: Nowotny M]]
-	[[Category: Rychlik~~, M P]]~~	+	[[Category: Rychlik MP]]
-	~~[[Category: Hydrolase-dna-rna hybrid complex]]~~	+
-	~~[[Category: Nuclease]]~~	+
-	~~[[Category: Rnase h~~]]	+

OCA at 07:14, 12 May 2022

OCA — Thu, 12 May 2022 07:14:53 GMT

←Older revision		Revision as of 07:14, 12 May 2022
Line 1:		Line 1:

	==T. maritima RNase H2 D107N in complex with nucleic acid substrate and magnesium ions==		==T. maritima RNase H2 D107N in complex with nucleic acid substrate and magnesium ions==
-	<StructureSection load='3o3f' size='340' side='right' caption='[[3o3f]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='3o3f' size='340' side='right'caption='[[3o3f]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3o3f]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_43589 Atcc 43589]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O3F OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3O3F FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3o3f]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_43589 Atcc 43589]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O3F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3O3F FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3o3g\|3o3g]], [[3o3h\|3o3h]], [[2etj\|2etj]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3o3g\|3o3g]], [[3o3h\|3o3h]], [[2etj\|2etj]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rnhB, TM_0915 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2336 ATCC 43589])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rnhB, TM_0915 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2336 ATCC 43589])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Ribonuclease_H Ribonuclease H], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.4 3.1.26.4] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Ribonuclease_H Ribonuclease H], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.4 3.1.26.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3o3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o3f OCA], [~~http~~://pdbe.org/3o3f PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3o3f RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3o3f PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3o3f ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3o3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o3f OCA], [https://pdbe.org/3o3f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3o3f RCSB], [https://www.ebi.ac.uk/pdbsum/3o3f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3o3f ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/RNH2_THEMA RNH2_THEMA]] Endonuclease that specifically degrades the RNA of RNA-DNA hybrids (By similarity).	+	[[https://www.uniprot.org/uniprot/RNH2_THEMA RNH2_THEMA]] Endonuclease that specifically degrades the RNA of RNA-DNA hybrids (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:

	==See Also==		==See Also==
-	*[[Ribonuclease\|Ribonuclease]]	+	*[[Ribonuclease 3D structures\|Ribonuclease 3D structures]]
-	*[[Temp\|Temp]]	+
	== References ==		== References ==
	<references/>		<references/>
Line 30:		Line 29:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 43589]]		[[Category: Atcc 43589]]
		+	[[Category: Large Structures]]
	[[Category: Ribonuclease H]]		[[Category: Ribonuclease H]]
	[[Category: Cerritelli, S M]]		[[Category: Cerritelli, S M]]

OCA at 14:46, 4 August 2016

OCA — Thu, 04 Aug 2016 14:46:14 GMT

←Older revision		Revision as of 14:46, 4 August 2016
Line 1:		Line 1:
		+
	==T. maritima RNase H2 D107N in complex with nucleic acid substrate and magnesium ions==		==T. maritima RNase H2 D107N in complex with nucleic acid substrate and magnesium ions==
	<StructureSection load='3o3f' size='340' side='right' caption='[[3o3f]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='3o3f' size='340' side='right' caption='[[3o3f]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3o3f]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Thermotoga_maritima Thermotoga maritima~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O3F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3O3F FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3o3f]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43589 Atcc 43589]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O3F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3O3F FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3o3g\|3o3g]], [[3o3h\|3o3h]], [[2etj\|2etj]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3o3g\|3o3g]], [[3o3h\|3o3h]], [[2etj\|2etj]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rnhB, TM_0915 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2336 ~~Thermotoga maritima~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rnhB, TM_0915 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2336 ATCC 43589])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_H Ribonuclease H], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.4 3.1.26.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_H Ribonuclease H], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.4 3.1.26.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3o3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o3f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3o3f RCSB], [http://www.ebi.ac.uk/pdbsum/3o3f PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3o3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o3f OCA], [http://pdbe.org/3o3f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3o3f RCSB], [http://www.ebi.ac.uk/pdbsum/3o3f PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3o3f ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3o3f" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Ribonuclease\|Ribonuclease]]		*[[Ribonuclease\|Ribonuclease]]
	*[[Temp\|Temp]]		*[[Temp\|Temp]]
-	*[[User:Jaime.Prilusky/Test/tree\|User:Jaime.Prilusky/Test/tree]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 43589]]
	[[Category: Ribonuclease H]]		[[Category: Ribonuclease H]]
-	[[Category: Thermotoga maritima]]
	[[Category: Cerritelli, S M]]		[[Category: Cerritelli, S M]]
	[[Category: Chon, H]]		[[Category: Chon, H]]

OCA at 10:14, 25 December 2014

OCA — Thu, 25 Dec 2014 10:14:19 GMT

←Older revision		Revision as of 10:14, 25 December 2014
Line 9:		Line 9:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3o3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o3f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3o3f RCSB], [http://www.ebi.ac.uk/pdbsum/3o3f PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3o3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o3f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3o3f RCSB], [http://www.ebi.ac.uk/pdbsum/3o3f PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/RNH2_THEMA RNH2_THEMA]] Endonuclease that specifically degrades the RNA of RNA-DNA hybrids (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 22:
	==See Also==		==See Also==
	*[[Ribonuclease\|Ribonuclease]]		*[[Ribonuclease\|Ribonuclease]]
		+	*[[Temp\|Temp]]
	*[[User:Jaime.Prilusky/Test/tree\|User:Jaime.Prilusky/Test/tree]]		*[[User:Jaime.Prilusky/Test/tree\|User:Jaime.Prilusky/Test/tree]]
	== References ==		== References ==

OCA at 11:02, 9 December 2014

OCA — Tue, 09 Dec 2014 11:02:42 GMT

←Older revision		Revision as of 11:02, 9 December 2014
Line 1:		Line 1:
-	[[~~Image:~~3o3f.~~png~~\|~~left~~\|~~200px~~]]	+	==T. maritima RNase H2 D107N in complex with nucleic acid substrate and magnesium ions==
		+	<StructureSection load='3o3f' size='340' side='right' caption='[[3o3f]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3o3f]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O3F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3O3F FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3o3g\|3o3g]], [[3o3h\|3o3h]], [[2etj\|2etj]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rnhB, TM_0915 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2336 Thermotoga maritima])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_H Ribonuclease H], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.4 3.1.26.4] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3o3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o3f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3o3f RCSB], [http://www.ebi.ac.uk/pdbsum/3o3f PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Two classes of RNase H hydrolyze RNA of RNA/DNA hybrids. In contrast to RNase H1 that requires four ribonucleotides for cleavage, RNase H2 can nick duplex DNAs containing a single ribonucleotide, suggesting different in vivo substrates. We report here the crystal structures of a type 2 RNase H in complex with substrates containing a (5')RNA-DNA(3') junction. They revealed a unique mechanism of recognition and substrate-assisted cleavage. A conserved tyrosine residue distorts the nucleic acid at the junction, allowing the substrate to function in catalysis by participating in coordination of the active site metal ion. The biochemical and structural properties of RNase H2 explain the preference of the enzyme for junction substrates and establish the structural and mechanistic differences with RNase H1. Junction recognition is important for the removal of RNA embedded in DNA and may play an important role in DNA replication and repair.

-	~~{{STRUCTURE_3o3f\| PDB=3o3f \| SCENE= }}~~	+	Crystal Structures of RNase H2 in Complex with Nucleic Acid Reveal the Mechanism of RNA-DNA Junction Recognition and Cleavage.,Rychlik MP, Chon H, Cerritelli SM, Klimek P, Crouch RJ, Nowotny M Mol Cell. 2010 Nov 24;40(4):658-70. PMID:21095591<ref>PMID:21095591</ref>

-	~~===T. maritima RNase H2 D107N in complex with nucleic acid substrate and magnesium ions===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_21095591}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3o3f]] is~~ a ~~3 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Thermotoga_maritima Thermotoga maritima]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3O3F OCA].~~	+

	==See Also==		==See Also==
	*[[Ribonuclease\|Ribonuclease]]		*[[Ribonuclease\|Ribonuclease]]
-		+	*[[User:Jaime.Prilusky/Test/tree\|User:Jaime.Prilusky/Test/tree]]
-	==~~Reference~~==	+	== References ==
-	<~~ref group="xtra">PMID:021095591<~~/~~ref~~><~~references group="xtra"~~/>	+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Ribonuclease H]]		[[Category: Ribonuclease H]]
	[[Category: Thermotoga maritima]]		[[Category: Thermotoga maritima]]
-	[[Category: Cerritelli, S M.]]	+	[[Category: Cerritelli, S M]]
-	[[Category: Chon, H.]]	+	[[Category: Chon, H]]
-	[[Category: Crouch, R J.]]	+	[[Category: Crouch, R J]]
-	[[Category: Klimek, P.]]	+	[[Category: Klimek, P]]
-	[[Category: Nowotny, M.]]	+	[[Category: Nowotny, M]]
-	[[Category: Rychlik, M P.]]	+	[[Category: Rychlik, M P]]
	[[Category: Hydrolase-dna-rna hybrid complex]]		[[Category: Hydrolase-dna-rna hybrid complex]]
	[[Category: Nuclease]]		[[Category: Nuclease]]
	[[Category: Rnase h]]		[[Category: Rnase h]]

OCA at 15:31, 20 October 2012

OCA — Sat, 20 Oct 2012 15:31:08 GMT

←Older revision		Revision as of 15:31, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3o3f]] is a 3 chain structure ~~of [[Ribonuclease]]~~ with sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O3F OCA].	+	[[3o3f]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O3F OCA].

	==See Also==		==See Also==

OCA: Protected "3o3f" [edit=sysop:move=sysop]

OCA — Thu, 26 Jul 2012 18:38:12 GMT

Protected "3o3f" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:38, 26 July 2012

OCA at 18:38, 26 July 2012

OCA — Thu, 26 Jul 2012 18:38:11 GMT

←Older revision		Revision as of 18:38, 26 July 2012
Line 1:		Line 1:
-	~~{{Seed}}~~	+	[[Image:3o3f.png\|left\|200px]]
-	[[Image:3o3f.~~jpg~~\|left\|200px]]	+

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3o3f", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3o3f\| PDB=3o3f \| SCENE= }}		{{STRUCTURE_3o3f\| PDB=3o3f \| SCENE= }}

	===T. maritima RNase H2 D107N in complex with nucleic acid substrate and magnesium ions===		===T. maritima RNase H2 D107N in complex with nucleic acid substrate and magnesium ions===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_21095591}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 21095591 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_21095591}}		{{ABSTRACT_PUBMED_21095591}}

	==About this Structure==		==About this Structure==
-	~~3O3F~~ is a 3 ~~chains~~ structure with ~~sequences~~ from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O3F OCA].	+	[[3o3f]] is a 3 chain structure of [[Ribonuclease]] with sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O3F OCA].
		+
		+	==See Also==
		+	*[[Ribonuclease\|Ribonuclease]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~21095591~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:021095591</ref><references group="xtra"/>
	[[Category: Ribonuclease H]]		[[Category: Ribonuclease H]]
	[[Category: Thermotoga maritima]]		[[Category: Thermotoga maritima]]
Line 35:		Line 26:
	[[Category: Nuclease]]		[[Category: Nuclease]]
	[[Category: Rnase h]]		[[Category: Rnase h]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 8 11:51:34 2010''

OCA at 08:39, 8 December 2010

OCA — Wed, 08 Dec 2010 08:39:33 GMT

←Older revision		Revision as of 08:39, 8 December 2010
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3o3f.jpg\|left\|200px]]

-	The ~~entry 3o3f~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3o3f", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3o3f\| PDB=3o3f \| SCENE= }}

-	~~Authors: Rychlik, M.P., Chon, H., Cerritelli, S.M., Klimek, P., Crouch, R.J., Nowotny, M~~.	+	===T. maritima RNase H2 D107N in complex with nucleic acid substrate and magnesium ions===

-	Description: T. maritima RNase H2 D107N in complex with nucleic acid substrate and magnesium ions

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Aug 18~~ 11:24:33 2010''	+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21095591}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21095591 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21095591}}
		+
		+	==About this Structure==
		+	3O3F is a 3 chains structure with sequences from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O3F OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:21095591</ref><references group="xtra"/>
		+	[[Category: Ribonuclease H]]
		+	[[Category: Thermotoga maritima]]
		+	[[Category: Cerritelli, S M.]]
		+	[[Category: Chon, H.]]
		+	[[Category: Crouch, R J.]]
		+	[[Category: Klimek, P.]]
		+	[[Category: Nowotny, M.]]
		+	[[Category: Rychlik, M P.]]
		+	[[Category: Hydrolase-dna-rna hybrid complex]]
		+	[[Category: Nuclease]]
		+	[[Category: Rnase h]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 8 11:51:34 2010''

OCA at 07:16, 18 August 2010

OCA — Wed, 18 Aug 2010 07:16:59 GMT

←Older revision		Revision as of 07:16, 18 August 2010
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3o3f is ON HOLD	+	The entry 3o3f is ON HOLD until Paper Publication

	Authors: Rychlik, M.P., Chon, H., Cerritelli, S.M., Klimek, P., Crouch, R.J., Nowotny, M.		Authors: Rychlik, M.P., Chon, H., Cerritelli, S.M., Klimek, P., Crouch, R.J., Nowotny, M.
Line 7:		Line 7:
	Description: T. maritima RNase H2 D107N in complex with nucleic acid substrate and magnesium ions		Description: T. maritima RNase H2 D107N in complex with nucleic acid substrate and magnesium ions

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug ~~4 08~~:52:37 2010''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug 18 11:24:33 2010''