3o4q - Revision history

OCA at 07:58, 12 July 2023

OCA — Wed, 12 Jul 2023 07:58:31 GMT

←Older revision		Revision as of 07:58, 12 July 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3o4q]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rous_sarcoma_virus Rous sarcoma virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O4Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3O4Q FirstGlance]. <br>		<table><tr><td colspan='2'>[[3o4q]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rous_sarcoma_virus Rous sarcoma virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O4Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3O4Q FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.55Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~OCY~~:~~HYDROXYETHYLCYSTEINE~~'>~~OCY~~</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=OCY:HYDROXYETHYLCYSTEINE'>OCY</scene></td></tr>
-	~~<tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;~~'>~~[[3o4n\|3o4n]]~~</~~div~~>~~</td></tr>~~	+
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pol ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11886 Rous sarcoma virus])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3o4q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o4q OCA], [https://pdbe.org/3o4q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3o4q RCSB], [https://www.ebi.ac.uk/pdbsum/3o4q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3o4q ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3o4q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o4q OCA], [https://pdbe.org/3o4q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3o4q RCSB], [https://www.ebi.ac.uk/pdbsum/3o4q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3o4q ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/POL_RSVSB POL_RSVSB] Capsid protein p27: Self-associates to form the irregular polyhedron core composed of hexamers and pentamers, that encapsulates the genomic RNA-nucleocapsid complex. Assembles as a tube in vitro. Binds to inositol hexakisphosphate (IP6), which allows the assembly of the polyhedral capsid.[UniProtKB:P03322] Plays a role in the oligomerization of the Gag polyprotein and in the stabilization of the immature particle. Essential layering element during tube assembly. Allows the cooperative binging of Gag to the host plasma membrane.[UniProtKB:P03322] Binds strongly to viral nucleic acids and promotes their packaging (By similarity). Plays a role in the maturation-stabilization of the viral dimeric RNA via highly structured zinc-binding motifs (By similarity).[UniProtKB:P03322][UniProtKB:P0C776] The aspartyl protease mediates proteolytic cleavages of Gag and Gag-Pol polyproteins during or shortly after the release of the virion from the plasma membrane. Cleavages take place as an ordered, step-wise cascade to yield mature proteins. This process is called maturation. Displays maximal activity during the budding process just prior to particle release from the cell.[PROSITE-ProRule:PRU00275] Catalyzes viral DNA integration into the host chromosome, by performing a series of DNA cutting and joining reactions (PubMed:9218451). This recombination event is an essential step in the viral replication cycle. Has a strong preference for using the 3'-OH at the viral DNA end as a nucleophile.[UniProtKB:P03354]<ref>PMID:9218451</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Rous sarcoma virus]]		[[Category: Rous sarcoma virus]]
-	[[Category: Ballandras, A]]	+	[[Category: Ballandras A]]
-	[[Category: Gouet, P]]	+	[[Category: Gouet P]]
-	[[Category: Robert, X]]	+	[[Category: Robert X]]
-	~~[[Category: Dna binding protein]]~~	+
-	~~[[Category: Dna integration process~~]]	+

OCA at 05:44, 6 October 2021

OCA — Wed, 06 Oct 2021 05:44:17 GMT

←Older revision		Revision as of 05:44, 6 October 2021
Line 1:		Line 1:

	==Crystal structure of the Rous Associated Virus Integrase catalytic domain A182T in citrate buffer pH 6.2==		==Crystal structure of the Rous Associated Virus Integrase catalytic domain A182T in citrate buffer pH 6.2==
-	<StructureSection load='3o4q' size='340' side='right' caption='[[3o4q]], [[Resolution\|resolution]] 1.55Å' scene=''>	+	<StructureSection load='3o4q' size='340' side='right'caption='[[3o4q]], [[Resolution\|resolution]] 1.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3o4q]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Rous_sarcoma_virus Rous sarcoma virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O4Q OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3O4Q FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3o4q]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rous_sarcoma_virus Rous sarcoma virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O4Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3O4Q FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=OCY:HYDROXYETHYLCYSTEINE'>OCY</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=OCY:HYDROXYETHYLCYSTEINE'>OCY</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3o4n\|3o4n]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3o4n\|3o4n]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pol ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11886 Rous sarcoma virus])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pol ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11886 Rous sarcoma virus])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3o4q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o4q OCA], [~~http~~://pdbe.org/3o4q PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3o4q RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3o4q PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3o4q ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3o4q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o4q OCA], [https://pdbe.org/3o4q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3o4q RCSB], [https://www.ebi.ac.uk/pdbsum/3o4q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3o4q ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 21:		Line 21:

	==See Also==		==See Also==
-	*[[Retroviral ~~Integrase~~\|Retroviral ~~Integrase~~]]	+	*[[Retroviral integrase 3D structures\|Retroviral integrase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Rous sarcoma virus]]		[[Category: Rous sarcoma virus]]
	[[Category: Ballandras, A]]		[[Category: Ballandras, A]]

OCA at 14:28, 4 August 2016

OCA — Thu, 04 Aug 2016 14:28:21 GMT

←Older revision		Revision as of 14:28, 4 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of the Rous Associated Virus Integrase catalytic domain A182T in citrate buffer pH 6.2==		==Crystal structure of the Rous Associated Virus Integrase catalytic domain A182T in citrate buffer pH 6.2==
	<StructureSection load='3o4q' size='340' side='right' caption='[[3o4q]], [[Resolution\|resolution]] 1.55Å' scene=''>		<StructureSection load='3o4q' size='340' side='right' caption='[[3o4q]], [[Resolution\|resolution]] 1.55Å' scene=''>
Line 7:		Line 8:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3o4n\|3o4n]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3o4n\|3o4n]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pol ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11886 Rous sarcoma virus])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pol ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11886 Rous sarcoma virus])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3o4q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o4q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3o4q RCSB], [http://www.ebi.ac.uk/pdbsum/3o4q PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3o4q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o4q OCA], [http://pdbe.org/3o4q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3o4q RCSB], [http://www.ebi.ac.uk/pdbsum/3o4q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3o4q ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3o4q" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 23:24, 3 January 2015

OCA — Sat, 03 Jan 2015 23:24:38 GMT

←Older revision		Revision as of 23:24, 3 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3o4q]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Rous_sarcoma_virus Rous sarcoma virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O4Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3O4Q FirstGlance]. <br>		<table><tr><td colspan='2'>[[3o4q]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Rous_sarcoma_virus Rous sarcoma virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O4Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3O4Q FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=OCY:HYDROXYETHYLCYSTEINE'>OCY</scene></td></tr>	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=OCY:HYDROXYETHYLCYSTEINE'>OCY</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3o4n\|3o4n]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3o4n\|3o4n]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pol ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11886 Rous sarcoma virus])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pol ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11886 Rous sarcoma virus])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3o4q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o4q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3o4q RCSB], [http://www.ebi.ac.uk/pdbsum/3o4q PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3o4q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o4q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3o4q RCSB], [http://www.ebi.ac.uk/pdbsum/3o4q PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 15:		Line 15:
	A crystal structure of the catalytic core domain of an avian sarcoma and leukemia virus integrase suggests an alternate dimeric assembly.,Ballandras A, Moreau K, Robert X, Confort MP, Merceron R, Haser R, Ronfort C, Gouet P PLoS One. 2011;6(8):e23032. Epub 2011 Aug 9. PMID:21857987<ref>PMID:21857987</ref>		A crystal structure of the catalytic core domain of an avian sarcoma and leukemia virus integrase suggests an alternate dimeric assembly.,Ballandras A, Moreau K, Robert X, Confort MP, Merceron R, Haser R, Ronfort C, Gouet P PLoS One. 2011;6(8):e23032. Epub 2011 Aug 9. PMID:21857987<ref>PMID:21857987</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+
		+	==See Also==
		+	*[[Retroviral Integrase\|Retroviral Integrase]]
	== References ==		== References ==
	<references/>		<references/>
Line 22:		Line 25:
	</StructureSection>		</StructureSection>
	[[Category: Rous sarcoma virus]]		[[Category: Rous sarcoma virus]]
-	[[Category: Ballandras, A.]]	+	[[Category: Ballandras, A]]
-	[[Category: Gouet, P.]]	+	[[Category: Gouet, P]]
-	[[Category: Robert, X.]]	+	[[Category: Robert, X]]
	[[Category: Dna binding protein]]		[[Category: Dna binding protein]]
	[[Category: Dna integration process]]		[[Category: Dna integration process]]

OCA at 10:44, 28 May 2014

OCA — Wed, 28 May 2014 10:44:04 GMT

←Older revision		Revision as of 10:44, 28 May 2014
Line 1:		Line 1:
-	[[~~Image:~~3o4q.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of the Rous Associated Virus Integrase catalytic domain A182T in citrate buffer pH 6.2==
		+	<StructureSection load='3o4q' size='340' side='right' caption='[[3o4q]], [[Resolution\|resolution]] 1.55Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3o4q]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Rous_sarcoma_virus Rous sarcoma virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O4Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3O4Q FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=OCY:HYDROXYETHYLCYSTEINE'>OCY</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3o4n\|3o4n]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pol ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11886 Rous sarcoma virus])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3o4q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o4q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3o4q RCSB], [http://www.ebi.ac.uk/pdbsum/3o4q PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Integrase (IN) is an important therapeutic target in the search for anti-Human Immunodeficiency Virus (HIV) inhibitors. This enzyme is composed of three domains and is hard to crystallize in its full form. First structural results on IN were obtained on the catalytic core domain (CCD) of the avian Rous and Sarcoma Virus strain Schmidt-Ruppin A (RSV-A) and on the CCD of HIV-1 IN. A ribonuclease-H like motif was revealed as well as a dimeric interface stabilized by two pairs of alpha-helices (alpha1/alpha5, alpha5/alpha1). These structural features have been validated in other structures of IN CCDs. We have determined the crystal structure of the Rous-associated virus type-1 (RAV-1) IN CCD to 1.8 A resolution. RAV-1 IN shows a standard activity for integration and its CCD differs in sequence from that of RSV-A by a single accessible residue in position 182 (substitution A182T). Surprisingly, the CCD of RAV-1 IN associates itself with an unexpected dimeric interface characterized by three pairs of alpha-helices (alpha3/alpha5, alpha1/alpha1, alpha5/alpha3). A182 is not involved in this novel interface, which results from a rigid body rearrangement of the protein at its alpha1, alpha3, alpha5 surface. A new basic groove that is suitable for single-stranded nucleic acid binding is observed at the surface of the dimer. We have subsequently determined the structure of the mutant A182T of RAV-1 IN CCD and obtained a RSV-A IN CCD-like structure with two pairs of buried alpha-helices at the interface. Our results suggest that the CCD of avian INs can dimerize in more than one state. Such flexibility can further explain the multifunctionality of retroviral INs, which beside integration of dsDNA are implicated in different steps of the retroviral cycle in presence of viral ssRNA.

-	~~<!--~~	+	A crystal structure of the catalytic core domain of an avian sarcoma and leukemia virus integrase suggests an alternate dimeric assembly.,Ballandras A, Moreau K, Robert X, Confort MP, Merceron R, Haser R, Ronfort C, Gouet P PLoS One. 2011;6(8):e23032. Epub 2011 Aug 9. PMID:21857987<ref>PMID:21857987</ref>
-	~~The line below this paragraph~~, ~~containing "STRUCTURE_3o4q"~~, ~~creates the "Structure Box" on the page~~.	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_3o4q\| PDB=3o4q \| SCENE= }}~~	+

-	~~===Crystal structure~~ of the ~~Rous Associated Virus Integrase catalytic domain A182T in citrate buffer pH 6~~.~~2===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_21857987}}, adds the Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 21857987 is the PubMed ID number.~~	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_21857987}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3o4q]] is a 1 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Rous_sarcoma_virus Rous sarcoma virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O4Q OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:021857987<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Rous sarcoma virus]]		[[Category: Rous sarcoma virus]]
	[[Category: Ballandras, A.]]		[[Category: Ballandras, A.]]

OCA at 05:45, 11 January 2012

OCA — Wed, 11 Jan 2012 05:45:34 GMT

←Older revision		Revision as of 05:45, 11 January 2012
Line 1:		Line 1:
-	[[Image:3o4q.~~jpg~~\|left\|200px]]	+	[[Image:3o4q.png\|left\|200px]]

	<!--		<!--
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	===Crystal structure of the Rous Associated Virus Integrase catalytic domain A182T in citrate buffer pH 6.2===		===Crystal structure of the Rous Associated Virus Integrase catalytic domain A182T in citrate buffer pH 6.2===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21857987}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21857987 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21857987}}

	==About this Structure==		==About this Structure==
	[[3o4q]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Rous_sarcoma_virus Rous sarcoma virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O4Q OCA].		[[3o4q]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Rous_sarcoma_virus Rous sarcoma virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O4Q OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:021857987</ref><references group="xtra"/>
	[[Category: Rous sarcoma virus]]		[[Category: Rous sarcoma virus]]
	[[Category: Ballandras, A.]]		[[Category: Ballandras, A.]]

OCA at 06:53, 31 August 2011

OCA — Wed, 31 Aug 2011 06:53:45 GMT

←Older revision		Revision as of 06:53, 31 August 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3o4q.jpg\|left\|200px]]

-	The ~~entry 3o4q~~ is ~~ON HOLD~~ ~~until Aug 27 2012~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3o4q", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3o4q\| PDB=3o4q \| SCENE= }}

-	~~Authors: Ballandras, A., Robert, X., Gouet, P~~.	+	===Crystal structure of the Rous Associated Virus Integrase catalytic domain A182T in citrate buffer pH 6.2===

-	~~Description: Crystal~~ structure ~~of the~~ Rous ~~Associated Virus Integrase catalytic domain A182T in citrate buffer pH 6~~.2	+
		+	==About this Structure==
		+	[[3o4q]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Rous_sarcoma_virus Rous sarcoma virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O4Q OCA].
		+	[[Category: Rous sarcoma virus]]
		+	[[Category: Ballandras, A.]]
		+	[[Category: Gouet, P.]]
		+	[[Category: Robert, X.]]
		+	[[Category: Dna binding protein]]
		+	[[Category: Dna integration process]]

OCA: Protected "3o4q" [edit=sysop:move=sysop]

OCA — Wed, 03 Aug 2011 08:12:44 GMT

Protected "3o4q" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:12, 3 August 2011

OCA at 08:12, 3 August 2011

OCA — Wed, 03 Aug 2011 08:12:43 GMT

←Older revision		Revision as of 08:12, 3 August 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3o4q is ON HOLD until ~~Paper Publication~~	+	The entry 3o4q is ON HOLD until Aug 27 2012

	Authors: Ballandras, A., Robert, X., Gouet, P.		Authors: Ballandras, A., Robert, X., Gouet, P.

	Description: Crystal structure of the Rous Associated Virus Integrase catalytic domain A182T in citrate buffer pH 6.2		Description: Crystal structure of the Rous Associated Virus Integrase catalytic domain A182T in citrate buffer pH 6.2
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug 18 11:24:47 2010''

OCA at 07:17, 18 August 2010

OCA — Wed, 18 Aug 2010 07:17:14 GMT

←Older revision		Revision as of 07:17, 18 August 2010
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3o4q is ON HOLD	+	The entry 3o4q is ON HOLD until Paper Publication

	Authors: Ballandras, A., Robert, X., Gouet, P.		Authors: Ballandras, A., Robert, X., Gouet, P.

-	Description: Crystal structure of the ~~catalytic core domain of the Avian Leukemia and Sarcoma~~ Virus Integrase (A182T ~~mutant)~~	+	Description: Crystal structure of the Rous Associated Virus Integrase catalytic domain A182T in citrate buffer pH 6.2

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug ~~4 08~~:53:28 2010''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug 18 11:24:47 2010''