3ojg - Revision history

OCA at 11:16, 21 February 2024

OCA — Wed, 21 Feb 2024 11:16:58 GMT

←Older revision		Revision as of 11:16, 21 February 2024
Line 3:		Line 3:
	<StructureSection load='3ojg' size='340' side='right'caption='[[3ojg]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='3ojg' size='340' side='right'caption='[[3ojg]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3ojg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_kaustophilus"_prickett_1928 "bacillus~~ kaustophilus~~" prickett 1928~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OJG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OJG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3ojg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_kaustophilus Geobacillus kaustophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OJG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OJG FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=HL4:N-[(3S)-2-OXOTETRAHYDROFURAN-3-YL]BUTANAMIDE'>HL4</scene>, ~~<scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.6Å</td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=HL4:N-[(3S)-2-OXOTETRAHYDROFURAN-3-YL]BUTANAMIDE'>HL4</scene>, <scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">GK1506 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1462 "Bacillus kaustophilus" Prickett 1928])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ojg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ojg OCA], [https://pdbe.org/3ojg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ojg RCSB], [https://www.ebi.ac.uk/pdbsum/3ojg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ojg ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ojg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ojg OCA], [https://pdbe.org/3ojg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ojg RCSB], [https://www.ebi.ac.uk/pdbsum/3ojg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ojg ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/Q5KZU5_GEOKA Q5KZU5_GEOKA]
-	~~A thermostable quorum-quenching lactonase from Geobacillus kaustophilus HTA426 (GI~~: 56420041) was used as an initial template for in vitro directed evolution experiments. This enzyme belongs to the phosphotriesterase-like lactonase (PLL) group of enzymes within the amidohydrolase superfamily that hydrolyze N-acylhomoserine lactones (AHLs) that are involved in virulence pathways of quorum-sensing pathogenic bacteria. Here we have determined the N-butyryl-L-homoserine lactone-liganded structure of the catalytically inactive D266N mutant of this enzyme to a resolution of 1.6 A. Using a tunable, bioluminescence-based quorum-quenching molecular circuit, the catalytic efficiency was enhanced, and the AHL substrate range increased through two point mutations on the loops at the C-terminal ends of the third and seventh beta-strands. This E101N/~~R230I mutant had an increased value of k(cat)~~/~~K(m) of 72-fold toward 3-oxo-N-dodecanoyl-L-homoserine lactone~~. ~~The evolved mutant also exhibited lactonase activity toward N-butyryl-L-homoserine lactone, an AHL that was previously not hydrolyzed by the wild-type enzyme~~. Both the purified wild-type and mutant enzymes contain a mixture of zinc and iron and are colored purple and brown, respectively, at high concentrations. The origin of this coloration is suggested to be because of a charge transfer complex involving the beta-cation and Tyr-99 within the enzyme active site. Modulation of the charge transfer complex alters the lactonase activity of the mutant enzymes and is reflected in enzyme coloration changes. We attribute the observed enhancement in catalytic reactivity of the evolved enzyme to favorable modulations of the active site architecture toward productive geometries required for chemical catalysis.	+
-		+
-	Directed evolution of a thermostable quorum-quenching lactonase from the amidohydrolase superfamily.,Chow JY, Xue B, Lee KH, Tung A, Wu L, Robinson RC, Yew WS J Biol Chem. 2010 Dec 24;285(52):40911-20. Epub 2010 Oct 27. PMID:20980257<ref>PMID:20980257</~~ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3ojg" style="background-color:#fffaf0;"><~~/~~div>~~	+

	==See Also==		==See Also==
	*[[Phosphotriesterase 3D structures\|Phosphotriesterase 3D structures]]		*[[Phosphotriesterase 3D structures\|Phosphotriesterase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ kaustophilus ~~prickett 1928~~]]	+	[[Category: Geobacillus kaustophilus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Chow~~, J Y~~]]	+	[[Category: Chow JY]]
-	[[Category: Robinson~~, R C~~]]	+	[[Category: Robinson RC]]
-	[[Category: Tung, A]]	+	[[Category: Tung A]]
-	[[Category: Xue, B]]	+	[[Category: Xue B]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Lactonase~~]]	+

OCA at 10:45, 18 May 2022

OCA — Wed, 18 May 2022 10:45:35 GMT

←Older revision		Revision as of 10:45, 18 May 2022
Line 1:		Line 1:

	==Structure of an inactive lactonase from Geobacillus kaustophilus with bound N-butyryl-DL-homoserine lactone==		==Structure of an inactive lactonase from Geobacillus kaustophilus with bound N-butyryl-DL-homoserine lactone==
-	<StructureSection load='3ojg' size='340' side='right' caption='[[3ojg]], [[Resolution\|resolution]] 1.60Å' scene=''>	+	<StructureSection load='3ojg' size='340' side='right'caption='[[3ojg]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3ojg]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_kaustophilus"_prickett_1928 "bacillus kaustophilus" prickett 1928]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OJG OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3OJG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3ojg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_kaustophilus"_prickett_1928 "bacillus kaustophilus" prickett 1928]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OJG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OJG FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=HL4:N-[(3S)-2-OXOTETRAHYDROFURAN-3-YL]BUTANAMIDE'>HL4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=HL4:N-[(3S)-2-OXOTETRAHYDROFURAN-3-YL]BUTANAMIDE'>HL4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GK1506 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1462 "Bacillus kaustophilus" Prickett 1928])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GK1506 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1462 "Bacillus kaustophilus" Prickett 1928])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3ojg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ojg OCA], [~~http~~://pdbe.org/3ojg PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3ojg RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3ojg PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3ojg ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ojg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ojg OCA], [https://pdbe.org/3ojg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ojg RCSB], [https://www.ebi.ac.uk/pdbsum/3ojg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ojg ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 18:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 3ojg" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3ojg" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Phosphotriesterase 3D structures\|Phosphotriesterase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 23:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus kaustophilus prickett 1928]]		[[Category: Bacillus kaustophilus prickett 1928]]
		+	[[Category: Large Structures]]
	[[Category: Chow, J Y]]		[[Category: Chow, J Y]]
	[[Category: Robinson, R C]]		[[Category: Robinson, R C]]

OCA at 08:45, 8 November 2017

OCA — Wed, 08 Nov 2017 08:45:49 GMT

←Older revision		Revision as of 08:45, 8 November 2017
Line 18:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 3ojg" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3ojg" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Phosphotriesterase\|Phosphotriesterase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 20:43, 11 August 2016

OCA — Thu, 11 Aug 2016 20:43:53 GMT

←Older revision		Revision as of 20:43, 11 August 2016
Line 1:		Line 1:
		+
	==Structure of an inactive lactonase from Geobacillus kaustophilus with bound N-butyryl-DL-homoserine lactone==		==Structure of an inactive lactonase from Geobacillus kaustophilus with bound N-butyryl-DL-homoserine lactone==
	<StructureSection load='3ojg' size='340' side='right' caption='[[3ojg]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='3ojg' size='340' side='right' caption='[[3ojg]], [[Resolution\|resolution]] 1.60Å' scene=''>
Line 6:		Line 7:
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GK1506 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1462 "Bacillus kaustophilus" Prickett 1928])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GK1506 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1462 "Bacillus kaustophilus" Prickett 1928])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ojg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ojg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ojg RCSB], [http://www.ebi.ac.uk/pdbsum/3ojg PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ojg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ojg OCA], [http://pdbe.org/3ojg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ojg RCSB], [http://www.ebi.ac.uk/pdbsum/3ojg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ojg ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3ojg" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 14:25, 5 January 2015

OCA — Mon, 05 Jan 2015 14:25:53 GMT

←Older revision		Revision as of 14:25, 5 January 2015
Line 1:		Line 1:
-	~~{{STRUCTURE_3ojg\| PDB=3ojg \| SCENE= }}~~	+	==Structure of an inactive lactonase from Geobacillus kaustophilus with bound N-butyryl-DL-homoserine lactone==
-	===Structure of an inactive lactonase from Geobacillus kaustophilus with bound N-butyryl-DL-homoserine lactone===	+	<StructureSection load='3ojg' size='340' side='right' caption='[[3ojg]], [[Resolution\|resolution]] 1.60Å' scene=''>
-	~~{{ABSTRACT_PUBMED_20980257}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3ojg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_kaustophilus"_prickett_1928 "bacillus kaustophilus" prickett 1928]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OJG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OJG FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=HL4:N-[(3S)-2-OXOTETRAHYDROFURAN-3-YL]BUTANAMIDE'>HL4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GK1506 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1462 "Bacillus kaustophilus" Prickett 1928])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ojg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ojg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ojg RCSB], [http://www.ebi.ac.uk/pdbsum/3ojg PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	A thermostable quorum-quenching lactonase from Geobacillus kaustophilus HTA426 (GI: 56420041) was used as an initial template for in vitro directed evolution experiments. This enzyme belongs to the phosphotriesterase-like lactonase (PLL) group of enzymes within the amidohydrolase superfamily that hydrolyze N-acylhomoserine lactones (AHLs) that are involved in virulence pathways of quorum-sensing pathogenic bacteria. Here we have determined the N-butyryl-L-homoserine lactone-liganded structure of the catalytically inactive D266N mutant of this enzyme to a resolution of 1.6 A. Using a tunable, bioluminescence-based quorum-quenching molecular circuit, the catalytic efficiency was enhanced, and the AHL substrate range increased through two point mutations on the loops at the C-terminal ends of the third and seventh beta-strands. This E101N/R230I mutant had an increased value of k(cat)/K(m) of 72-fold toward 3-oxo-N-dodecanoyl-L-homoserine lactone. The evolved mutant also exhibited lactonase activity toward N-butyryl-L-homoserine lactone, an AHL that was previously not hydrolyzed by the wild-type enzyme. Both the purified wild-type and mutant enzymes contain a mixture of zinc and iron and are colored purple and brown, respectively, at high concentrations. The origin of this coloration is suggested to be because of a charge transfer complex involving the beta-cation and Tyr-99 within the enzyme active site. Modulation of the charge transfer complex alters the lactonase activity of the mutant enzymes and is reflected in enzyme coloration changes. We attribute the observed enhancement in catalytic reactivity of the evolved enzyme to favorable modulations of the active site architecture toward productive geometries required for chemical catalysis.

-	~~==About this Structure==~~	+	Directed evolution of a thermostable quorum-quenching lactonase from the amidohydrolase superfamily.,Chow JY, Xue B, Lee KH, Tung A, Wu L, Robinson RC, Yew WS J Biol Chem. 2010 Dec 24;285(52):40911-20. Epub 2010 Oct 27. PMID:20980257<ref>PMID:20980257</ref>
-	~~[[3ojg]] is~~ a ~~1 chain structure with sequence~~ from ~~[http~~:~~//en~~.~~wikipedia~~.~~org/wiki/"bacillus_kaustophilus"_prickett_1928 "bacillus kaustophilus" prickett 1928]. Full crystallographic information is available from [http~~://~~oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=3OJG OCA].~~	+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

	==See Also==		==See Also==
	*[[Phosphotriesterase\|Phosphotriesterase]]		*[[Phosphotriesterase\|Phosphotriesterase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:020980257</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Bacillus kaustophilus prickett 1928]]		[[Category: Bacillus kaustophilus prickett 1928]]
-	[[Category: Chow, J Y.]]	+	[[Category: Chow, J Y]]
-	[[Category: Robinson, R C.]]	+	[[Category: Robinson, R C]]
-	[[Category: Tung, A.]]	+	[[Category: Tung, A]]
-	[[Category: Xue, B.]]	+	[[Category: Xue, B]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Lactonase]]		[[Category: Lactonase]]

OCA at 07:14, 26 March 2014

OCA — Wed, 26 Mar 2014 07:14:23 GMT

←Older revision		Revision as of 07:14, 26 March 2014
Line 1:		Line 1:
-	[[Image:3ojg.png\|left\|200px]]
-
	{{STRUCTURE_3ojg\| PDB=3ojg \| SCENE= }}		{{STRUCTURE_3ojg\| PDB=3ojg \| SCENE= }}
-
	===Structure of an inactive lactonase from Geobacillus kaustophilus with bound N-butyryl-DL-homoserine lactone===		===Structure of an inactive lactonase from Geobacillus kaustophilus with bound N-butyryl-DL-homoserine lactone===
-		+	{{ABSTRACT_PUBMED_20980257}}
-	{{~~ABSTRACT_PUBMED_020980257~~}}	+

	==About this Structure==		==About this Structure==
-	[[3ojg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Geobacillus_kaustophilus Geobacillus~~ kaustophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OJG OCA].	+	[[3ojg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_kaustophilus"_prickett_1928 "bacillus kaustophilus" prickett 1928]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OJG OCA].

	==See Also==		==See Also==
Line 14:		Line 10:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:020980257</ref><references group="xtra"/>	+	<ref group="xtra">PMID:020980257</ref><references group="xtra"/><references/>
-	[[Category: ~~Geobacillus~~ kaustophilus]]	+	[[Category: Bacillus kaustophilus prickett 1928]]
	[[Category: Chow, J Y.]]		[[Category: Chow, J Y.]]
	[[Category: Robinson, R C.]]		[[Category: Robinson, R C.]]

OCA at 22:24, 20 October 2012

OCA — Sat, 20 Oct 2012 22:24:49 GMT

←Older revision		Revision as of 22:24, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3ojg]] is a 1 chain structure ~~of [[Phosphotriesterase]]~~ with sequence from [http://en.wikipedia.org/wiki/Geobacillus_kaustophilus Geobacillus kaustophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OJG OCA].	+	[[3ojg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Geobacillus_kaustophilus Geobacillus kaustophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OJG OCA].

	==See Also==		==See Also==

OCA: Protected "3ojg" [edit=sysop:move=sysop]

OCA — Fri, 27 Jul 2012 16:13:22 GMT

Protected "3ojg" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:13, 27 July 2012

OCA at 16:13, 27 July 2012

OCA — Fri, 27 Jul 2012 16:13:20 GMT

←Older revision		Revision as of 16:13, 27 July 2012
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-	~~{{Seed}}~~	+	[[Image:3ojg.png\|left\|200px]]
-	[[Image:3ojg.~~jpg~~\|left\|200px]]	+

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3ojg", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3ojg\| PDB=3ojg \| SCENE= }}		{{STRUCTURE_3ojg\| PDB=3ojg \| SCENE= }}

	===Structure of an inactive lactonase from Geobacillus kaustophilus with bound N-butyryl-DL-homoserine lactone===		===Structure of an inactive lactonase from Geobacillus kaustophilus with bound N-butyryl-DL-homoserine lactone===

		+	{{ABSTRACT_PUBMED_020980257}}

	==About this Structure==		==About this Structure==
-	~~3OJG~~ is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Geobacillus_kaustophilus Geobacillus kaustophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OJG OCA].	+	[[3ojg]] is a 1 chain structure of [[Phosphotriesterase]] with sequence from [http://en.wikipedia.org/wiki/Geobacillus_kaustophilus Geobacillus kaustophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OJG OCA].
		+
		+	==See Also==
		+	*[[Phosphotriesterase\|Phosphotriesterase]]
		+
		+	==Reference==
		+	<ref group="xtra">PMID:020980257</ref><references group="xtra"/>
	[[Category: Geobacillus kaustophilus]]		[[Category: Geobacillus kaustophilus]]
	[[Category: Chow, J Y.]]		[[Category: Chow, J Y.]]
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	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Lactonase]]		[[Category: Lactonase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct 27 12:15:13 2010''

OCA at 09:04, 27 October 2010

OCA — Wed, 27 Oct 2010 09:04:52 GMT

←Older revision		Revision as of 09:04, 27 October 2010
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-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:3ojg.jpg\|left\|200px]]

-	The ~~entry 3ojg~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3ojg", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3ojg\| PDB=3ojg \| SCENE= }}

-	~~Authors: Xue, B., Chow, J.Y., Tung, A., Robinson, R.C.~~	+	===Structure of an inactive lactonase from Geobacillus kaustophilus with bound N-butyryl-DL-homoserine lactone===

-	Description: Structure of an inactive lactonase from Geobacillus kaustophilus with bound N-butyryl-DL-homoserine lactone

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct ~~13 10~~:31:21 2010''	+	==About this Structure==
		+	3OJG is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Geobacillus_kaustophilus Geobacillus kaustophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OJG OCA].
		+	[[Category: Geobacillus kaustophilus]]
		+	[[Category: Chow, J Y.]]
		+	[[Category: Robinson, R C.]]
		+	[[Category: Tung, A.]]
		+	[[Category: Xue, B.]]
		+	[[Category: Hydrolase]]
		+	[[Category: Lactonase]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct 27 12:15:13 2010''