3osp - Revision history

OCA at 16:57, 1 November 2023

OCA — Wed, 01 Nov 2023 16:57:27 GMT

←Older revision		Revision as of 16:57, 1 November 2023
Line 3:		Line 3:
	<StructureSection load='3osp' size='340' side='right'caption='[[3osp]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='3osp' size='340' side='right'caption='[[3osp]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3osp]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_18824 Atcc 18824~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OSP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OSP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3osp]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OSP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OSP FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=DCP:~~2~~-DEOXYCYTIDINE-~~5~~-TRIPHOSPHATE'>DCP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=3DR:1,2-DIDEOXYRIBOFURANOSE-5-PHOSPHATE'>3DR</scene>, <scene name='pdbligand=DOC:2,3-DIDEOXYCYTIDINE-5-MONOPHOSPHATE'>DOC</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3DR:1,2-DIDEOXYRIBOFURANOSE-5-PHOSPHATE'>3DR</scene>, <scene name='pdbligand=DCP:2-DEOXYCYTIDINE-5-TRIPHOSPHATE'>DCP</scene>, <scene name='pdbligand=DOC:2,3-DIDEOXYCYTIDINE-5-MONOPHOSPHATE'>DOC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">O6339, REV1, YOR346W ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3osp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3osp OCA], [https://pdbe.org/3osp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3osp RCSB], [https://www.ebi.ac.uk/pdbsum/3osp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3osp ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3osp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3osp OCA], [https://pdbe.org/3osp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3osp RCSB], [https://www.ebi.ac.uk/pdbsum/3osp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3osp ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/REV1_YEAST REV1_YEAST]] Deoxycytidyl transferase involved in DNA repair. Transfers a dCMP residue from dCTP to the 3'-end of a DNA primer in a template-dependent reaction. May assist in the first step in the bypass of abasic lesions by the insertion of a nucleotide opposite the lesion. Required for normal induction of mutations by physical and chemical agents. Involved in mitochondrial DNA mutagenesis.<ref>PMID:8751446</ref> <ref>PMID:11316789</ref> <ref>PMID:16452144</ref>	+	[https://www.uniprot.org/uniprot/REV1_YEAST REV1_YEAST] Deoxycytidyl transferase involved in DNA repair. Transfers a dCMP residue from dCTP to the 3'-end of a DNA primer in a template-dependent reaction. May assist in the first step in the bypass of abasic lesions by the insertion of a nucleotide opposite the lesion. Required for normal induction of mutations by physical and chemical agents. Involved in mitochondrial DNA mutagenesis.<ref>PMID:8751446</ref> <ref>PMID:11316789</ref> <ref>PMID:16452144</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 3osp" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3osp" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[DNA polymerase 3D structures\|DNA polymerase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 18824]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Aggarwal, A K~~]]	+	[[Category: Saccharomyces cerevisiae]]
-	[[Category: ~~Nair, D T~~]]	+	[[Category: Aggarwal AK]]
-	[[Category: ~~Abasic site]]~~	+	[[Category: Nair DT]]
-	~~[[Category: Damage bypass]]~~	+
-	~~[[Category: Dna]]~~	+
-	~~[[Category: Dna polymerase]]~~	+
-	~~[[Category: Nucleus]]~~	+
-	~~[[Category: Transferase-dna complex~~]]	+

OCA at 10:55, 18 May 2022

OCA — Wed, 18 May 2022 10:55:46 GMT

←Older revision		Revision as of 10:55, 18 May 2022
Line 1:		Line 1:

	==Structure of rev1==		==Structure of rev1==
-	<StructureSection load='3osp' size='340' side='right' caption='[[3osp]], [[Resolution\|resolution]] 2.50Å' scene=''>	+	<StructureSection load='3osp' size='340' side='right'caption='[[3osp]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3osp]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OSP OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3OSP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3osp]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OSP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OSP FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DCP:2-DEOXYCYTIDINE-5-TRIPHOSPHATE'>DCP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DCP:2-DEOXYCYTIDINE-5-TRIPHOSPHATE'>DCP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=3DR:1,2-DIDEOXYRIBOFURANOSE-5-PHOSPHATE'>3DR</scene>, <scene name='pdbligand=DOC:2,3-DIDEOXYCYTIDINE-5-MONOPHOSPHATE'>DOC</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=3DR:1,2-DIDEOXYRIBOFURANOSE-5-PHOSPHATE'>3DR</scene>, <scene name='pdbligand=DOC:2,3-DIDEOXYCYTIDINE-5-MONOPHOSPHATE'>DOC</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">O6339, REV1, YOR346W ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">O6339, REV1, YOR346W ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3osp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3osp OCA], [~~http~~://pdbe.org/3osp PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3osp RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3osp PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3osp ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3osp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3osp OCA], [https://pdbe.org/3osp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3osp RCSB], [https://www.ebi.ac.uk/pdbsum/3osp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3osp ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/REV1_YEAST REV1_YEAST]] Deoxycytidyl transferase involved in DNA repair. Transfers a dCMP residue from dCTP to the 3'-end of a DNA primer in a template-dependent reaction. May assist in the first step in the bypass of abasic lesions by the insertion of a nucleotide opposite the lesion. Required for normal induction of mutations by physical and chemical agents. Involved in mitochondrial DNA mutagenesis.<ref>PMID:8751446</ref> <ref>PMID:11316789</ref> <ref>PMID:16452144</ref>	+	[[https://www.uniprot.org/uniprot/REV1_YEAST REV1_YEAST]] Deoxycytidyl transferase involved in DNA repair. Transfers a dCMP residue from dCTP to the 3'-end of a DNA primer in a template-dependent reaction. May assist in the first step in the bypass of abasic lesions by the insertion of a nucleotide opposite the lesion. Required for normal induction of mutations by physical and chemical agents. Involved in mitochondrial DNA mutagenesis.<ref>PMID:8751446</ref> <ref>PMID:11316789</ref> <ref>PMID:16452144</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 25:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 18824]]		[[Category: Atcc 18824]]
		+	[[Category: Large Structures]]
	[[Category: Aggarwal, A K]]		[[Category: Aggarwal, A K]]
	[[Category: Nair, D T]]		[[Category: Nair, D T]]

OCA at 09:11, 5 August 2016

OCA — Fri, 05 Aug 2016 09:11:24 GMT

←Older revision		Revision as of 09:11, 5 August 2016
Line 1:		Line 1:
		+
	==Structure of rev1==		==Structure of rev1==
	<StructureSection load='3osp' size='340' side='right' caption='[[3osp]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='3osp' size='340' side='right' caption='[[3osp]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3osp]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Saccharomyces_cerevisiae Saccharomyces cerevisiae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OSP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OSP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3osp]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OSP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OSP FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DCP:2-DEOXYCYTIDINE-5-TRIPHOSPHATE'>DCP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DCP:2-DEOXYCYTIDINE-5-TRIPHOSPHATE'>DCP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=3DR:1,2-DIDEOXYRIBOFURANOSE-5-PHOSPHATE'>3DR</scene>, <scene name='pdbligand=DOC:2,3-DIDEOXYCYTIDINE-5-MONOPHOSPHATE'>DOC</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=3DR:1,2-DIDEOXYRIBOFURANOSE-5-PHOSPHATE'>3DR</scene>, <scene name='pdbligand=DOC:2,3-DIDEOXYCYTIDINE-5-MONOPHOSPHATE'>DOC</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">O6339, REV1, YOR346W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ~~Saccharomyces cerevisiae~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">O6339, REV1, YOR346W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3osp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3osp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3osp RCSB], [http://www.ebi.ac.uk/pdbsum/3osp PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3osp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3osp OCA], [http://pdbe.org/3osp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3osp RCSB], [http://www.ebi.ac.uk/pdbsum/3osp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3osp ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3osp" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Saccharomyces cerevisiae~~]]	+	[[Category: Atcc 18824]]
	[[Category: Aggarwal, A K]]		[[Category: Aggarwal, A K]]
	[[Category: Nair, D T]]		[[Category: Nair, D T]]

OCA at 01:04, 25 December 2014

OCA — Thu, 25 Dec 2014 01:04:09 GMT

←Older revision		Revision as of 01:04, 25 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3osp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3osp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3osp RCSB], [http://www.ebi.ac.uk/pdbsum/3osp PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3osp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3osp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3osp RCSB], [http://www.ebi.ac.uk/pdbsum/3osp PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/REV1_YEAST REV1_YEAST]] Deoxycytidyl transferase involved in DNA repair. Transfers a dCMP residue from dCTP to the 3'-end of a DNA primer in a template-dependent reaction. May assist in the first step in the bypass of abasic lesions by the insertion of a nucleotide opposite the lesion. Required for normal induction of mutations by physical and chemical agents. Involved in mitochondrial DNA mutagenesis.<ref>PMID:8751446</ref> <ref>PMID:11316789</ref> <ref>PMID:16452144</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 07:26, 19 December 2014

OCA — Fri, 19 Dec 2014 07:26:21 GMT

←Older revision		Revision as of 07:26, 19 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3osp~~\| ~~PDB~~=3osp \| ~~SCENE~~= }}	+	==Structure of rev1==
-	===~~Structure of rev1~~===	+	<StructureSection load='3osp' size='340' side='right' caption='[[3osp]], [[Resolution\|resolution]] 2.50Å' scene=''>
-	~~{{ABSTRACT_PUBMED_21167175}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3osp]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OSP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OSP FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DCP:2-DEOXYCYTIDINE-5-TRIPHOSPHATE'>DCP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=3DR:1,2-DIDEOXYRIBOFURANOSE-5-PHOSPHATE'>3DR</scene>, <scene name='pdbligand=DOC:2,3-DIDEOXYCYTIDINE-5-MONOPHOSPHATE'>DOC</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">O6339, REV1, YOR346W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3osp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3osp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3osp RCSB], [http://www.ebi.ac.uk/pdbsum/3osp PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Abasic (apurinic/apyrimidinic) sites are among the most abundant DNA lesions in humans, and they present a strong block to replication. They are also highly mutagenic because when replicative DNA polymerases manage to insert a nucleotide opposite the lesion, they prefer to insert an A. Rev1, a member of Y-family DNA polymerases, does not obey the A-rule. This enzyme inserts a C opposite an abasic lesion with much greater catalytic efficiency than an A, G, or T. We present here the structure of yeast Rev1 in ternary complex with DNA containing an abasic lesion and with dCTP as the incoming nucleotide. The structure reveals a mechanism of synthesis across an abasic lesion that differs from that in other polymerases. The lesion is driven to an extrahelical position, and the incorporation of a C is mediated by an arginine (Arg324) that is conserved in all known orthologs of Rev1, including humans. The hydrophobic cavity that normally accommodates the unmodified G is instead filled with water molecules. Since Gs are especially prone to depurination through a spontaneous hydrolysis of the glycosidic bond, the ability of Rev1 to stabilize an abasic lesion in its active site and employ a surrogate arginine to incorporate a C provides a unique means for the "error-free" bypass of this noninstructional lesion.

-	~~==Function==~~	+	DNA synthesis across an abasic lesion by yeast REV1 DNA polymerase.,Nair DT, Johnson RE, Prakash L, Prakash S, Aggarwal AK J Mol Biol. 2011 Feb 11;406(1):18-28. Epub 2010 Dec 15. PMID:21167175<ref>PMID:21167175</ref>
-	~~[[http://www.uniprot.org/uniprot/REV1_YEAST REV1_YEAST]] Deoxycytidyl transferase involved in~~ DNA ~~repair. Transfers a dCMP residue from dCTP to the 3'-end of a DNA primer in a template-dependent reaction. May assist in the first step in the bypass of~~ abasic ~~lesions by the insertion of a nucleotide opposite the~~ lesion~~. Required for normal induction of mutations~~ by ~~physical and chemical agents. Involved in mitochondrial~~ DNA ~~mutagenesis~~.~~<ref>PMID~~:~~8751446</ref> <ref>~~PMID:~~11316789</ref>~~ <ref>PMID:~~16452144~~</ref>	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[3osp]] is~~ a ~~3 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3OSP OCA].~~	+	</div>
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:021167175</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
-	[[Category: Aggarwal, A K.]]	+	[[Category: Aggarwal, A K]]
-	[[Category: Nair, D T.]]	+	[[Category: Nair, D T]]
	[[Category: Abasic site]]		[[Category: Abasic site]]
	[[Category: Damage bypass]]		[[Category: Damage bypass]]

OCA at 08:39, 2 May 2013

OCA — Thu, 02 May 2013 08:39:01 GMT

←Older revision		Revision as of 08:39, 2 May 2013
Line 1:		Line 1:
-	[[Image:3osp.jpg\|left\|200px]]
-
-	<!--
-	The line below this paragraph, containing "STRUCTURE_3osp", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3osp\| PDB=3osp \| SCENE= }}		{{STRUCTURE_3osp\| PDB=3osp \| SCENE= }}
-
	===Structure of rev1===		===Structure of rev1===
		+	{{ABSTRACT_PUBMED_21167175}}

-		+	==Function==
-	~~<!--~~	+	[[http://www.uniprot.org/uniprot/REV1_YEAST REV1_YEAST]] Deoxycytidyl transferase involved in DNA repair. Transfers a dCMP residue from dCTP to the 3'-end of a DNA primer in a template-dependent reaction. May assist in the first step in the bypass of abasic lesions by the insertion of a nucleotide opposite the lesion. Required for normal induction of mutations by physical and chemical agents. Involved in mitochondrial DNA mutagenesis.<ref>PMID:8751446</ref> <ref>PMID:11316789</ref> <ref>PMID:16452144</ref>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_21167175}}, adds the Publication Abstract to the page~~	+
-	~~(as it appears on PubMed at~~ http://www.~~pubmed~~.~~gov), where 21167175 is the PubMed ID number~~.	+
-	-->	+
-	~~{{ABSTRACT_PUBMED_21167175}}~~	+

	==About this Structure==		==About this Structure==
Line 22:		Line 10:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~21167175~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:021167175</ref><references group="xtra"/><references/>
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
	[[Category: Aggarwal, A K.]]		[[Category: Aggarwal, A K.]]
	[[Category: Nair, D T.]]		[[Category: Nair, D T.]]
		+	[[Category: Abasic site]]
		+	[[Category: Damage bypass]]
		+	[[Category: Dna]]
		+	[[Category: Dna polymerase]]
		+	[[Category: Nucleus]]
		+	[[Category: Transferase-dna complex]]

OCA at 05:18, 13 April 2011

OCA — Wed, 13 Apr 2011 05:18:08 GMT

←Older revision		Revision as of 05:18, 13 April 2011
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-	~~'''Unreleased structure'''~~	+	[[Image:3osp.jpg\|left\|200px]]

-	The ~~entry 3osp~~ is ~~ON HOLD until sometime in~~ the ~~future~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3osp", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3osp\| PDB=3osp \| SCENE= }}

-	~~Authors: Nair, D.T., Aggarwal, A.K.~~	+	===Structure of rev1===

-	~~Description~~: Structure ~~of rev1~~	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21167175}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21167175 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21167175}}
		+
		+	==About this Structure==
		+	[[3osp]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OSP OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:21167175</ref><references group="xtra"/>
		+	[[Category: Saccharomyces cerevisiae]]
		+	[[Category: Aggarwal, A K.]]
		+	[[Category: Nair, D T.]]

OCA: Protected "3osp" [edit=sysop:move=sysop]

OCA — Wed, 26 Jan 2011 12:41:08 GMT

Protected "3osp" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:41, 26 January 2011

OCA at 12:41, 26 January 2011

OCA — Wed, 26 Jan 2011 12:41:07 GMT

←Older revision		Revision as of 12:41, 26 January 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3osp is ON HOLD	+	The entry 3osp is ON HOLD until sometime in the future

	Authors: Nair, D.T., Aggarwal, A.K.		Authors: Nair, D.T., Aggarwal, A.K.

	Description: Structure of rev1		Description: Structure of rev1
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct 6 05:49:07 2010''

OCA at 02:39, 6 October 2010

OCA — Wed, 06 Oct 2010 02:39:36 GMT

←Older revision		Revision as of 02:39, 6 October 2010
Line 3:		Line 3:
	The entry 3osp is ON HOLD		The entry 3osp is ON HOLD

-	Authors: Nair, D. T., Aggarwal, A. K.	+	Authors: Nair, D.T., Aggarwal, A.K.

-	Description: rev1	+	Description: Structure of rev1

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Sep 22 14~~:34:30 2010''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct 6 05:49:07 2010''