3otk - Revision history

OCA at 11:18, 21 February 2024

OCA — Wed, 21 Feb 2024 11:18:46 GMT

←Older revision		Revision as of 11:18, 21 February 2024
Line 3:		Line 3:
	<StructureSection load='3otk' size='340' side='right'caption='[[3otk]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='3otk' size='340' side='right'caption='[[3otk]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3otk]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OTK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OTK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3otk]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OTK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OTK FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HTO:HEPTANE-1,2,3-TRIOL'>HTO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene~~>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Gcnt1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=HTO:HEPTANE-1,2,3-TRIOL'>HTO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Beta-1,3-galactosyl-O-glycosyl-glycoprotein_beta-1,6-N-_acetylglucosaminyltransferase Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N- acetylglucosaminyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.102 2.4.1.102] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3otk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3otk OCA], [https://pdbe.org/3otk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3otk RCSB], [https://www.ebi.ac.uk/pdbsum/3otk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3otk ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3otk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3otk OCA], [https://pdbe.org/3otk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3otk RCSB], [https://www.ebi.ac.uk/pdbsum/3otk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3otk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/GCNT1_MOUSE GCNT1_MOUSE]] Forms critical branches in O-glycans.<ref>PMID:7983056</ref>	+	[https://www.uniprot.org/uniprot/GCNT1_MOUSE GCNT1_MOUSE] Forms critical branches in O-glycans.<ref>PMID:7983056</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Leukocyte-type core 2 beta1,6-N-acetylglucosaminyltransferase (C2GnT-L) is an inverting, metal-ion-independent glycosyltransferase that catalyzes the formation of mucin-type core 2 O-glycans. C2GnT-L belongs to the GT-A fold, yet it lacks the metal ion binding DXD motif characteristic of other nucleoside disphosphate GT-A fold glycosyltransferases. To shed light on the basis for its metal ion independence, we have solved the X-ray crystal structure (2.3 A resolution) of a mutant form of C2GnT-L (C217S) in complex with the nucleotide sugar product UDP and, using site-directed mutagenesis, examined the roles of R378 and K401 in both substrate binding and catalysis. The structure shows that C2GnT-L exists in an "open" conformation and a "closed" conformation and that, in the latter, R378 and K401 interact with the beta-phosphate moiety of the bound UDP. The two conformations are likely to be important in catalysis, but the conformational changes that lead to their interconversion do not resemble the nucleotide-sugar-mediated loop ordering observed in other GT-A glycosyltransferases. R378 and K401 were found to be important in substrate binding and/or catalysis, an observation consistent with the suggestion that they serve the same role played by metal ion in all of the other GT-A glycosyltransferases studied to date. Notably, R378 and K401 appear to function in a manner similar to that of the arginine and lysine residues contained in the RX(4-5)K motif found in the retaining GT-B glycosyltransferases.	+
-		+
-	Structural and mechanistic characterization of leukocyte-type core 2 beta1,6-N-acetylglucosaminyltransferase: a metal-ion-independent GT-A glycosyltransferase.,Pak JE, Satkunarajah M, Seetharaman J, Rini JM J Mol Biol. 2011 Dec 16;414(5):798-811. Epub 2011 Oct 26. PMID:22056345<ref>PMID:22056345</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3otk" style="background-color:#fffaf0;"></div~~>	+

	==See Also==		==See Also==
Line 28:		Line 18:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N- acetylglucosaminyltransferase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lk3 transgenic mice~~]]	+	[[Category: Mus musculus]]
-	[[Category: Pak~~, J E~~]]	+	[[Category: Pak JE]]
-	[[Category: Rini~~, J M]]~~	+	[[Category: Rini JM]]
-	~~[[Category: Glycosyltransferase]]~~	+
-	~~[[Category: Golgi]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 10:56, 18 May 2022

OCA — Wed, 18 May 2022 10:56:49 GMT

←Older revision		Revision as of 10:56, 18 May 2022
Line 1:		Line 1:

	==Structure and mechanisim of core 2 beta1,6-n-acetylglucosaminyltransferase: a Metal-ion independent gt-a glycosyltransferase==		==Structure and mechanisim of core 2 beta1,6-n-acetylglucosaminyltransferase: a Metal-ion independent gt-a glycosyltransferase==
-	<StructureSection load='3otk' size='340' side='right' caption='[[3otk]], [[Resolution\|resolution]] 2.30Å' scene=''>	+	<StructureSection load='3otk' size='340' side='right'caption='[[3otk]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3otk]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OTK OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3OTK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3otk]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OTK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OTK FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HTO:HEPTANE-1,2,3-TRIOL'>HTO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HTO:HEPTANE-1,2,3-TRIOL'>HTO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Gcnt1 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Gcnt1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Beta-1,3-galactosyl-O-glycosyl-glycoprotein_beta-1,6-N-_acetylglucosaminyltransferase Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N- acetylglucosaminyltransferase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.102 2.4.1.102] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Beta-1,3-galactosyl-O-glycosyl-glycoprotein_beta-1,6-N-_acetylglucosaminyltransferase Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N- acetylglucosaminyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.102 2.4.1.102] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3otk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3otk OCA], [~~http~~://pdbe.org/3otk PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3otk RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3otk PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3otk ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3otk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3otk OCA], [https://pdbe.org/3otk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3otk RCSB], [https://www.ebi.ac.uk/pdbsum/3otk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3otk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/GCNT1_MOUSE GCNT1_MOUSE]] Forms critical branches in O-glycans.<ref>PMID:7983056</ref>	+	[[https://www.uniprot.org/uniprot/GCNT1_MOUSE GCNT1_MOUSE]] Forms critical branches in O-glycans.<ref>PMID:7983056</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:

	==See Also==		==See Also==
-	*[[Glycosyltransferase\|Glycosyltransferase]]	+	*[[Glycosyltransferase 3D structures\|Glycosyltransferase 3D structures]]
-	*[[O-GlcNAc transferase\|O-GlcNAc transferase]]	+	*[[O-GlcNAc transferase 3D structures\|O-GlcNAc transferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 29:		Line 29:
	</StructureSection>		</StructureSection>
	[[Category: Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N- acetylglucosaminyltransferase]]		[[Category: Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N- acetylglucosaminyltransferase]]
		+	[[Category: Large Structures]]
	[[Category: Lk3 transgenic mice]]		[[Category: Lk3 transgenic mice]]
	[[Category: Pak, J E]]		[[Category: Pak, J E]]

OCA at 18:27, 5 August 2016

OCA — Fri, 05 Aug 2016 18:27:30 GMT

←Older revision		Revision as of 18:27, 5 August 2016
Line 1:		Line 1:
		+
	==Structure and mechanisim of core 2 beta1,6-n-acetylglucosaminyltransferase: a Metal-ion independent gt-a glycosyltransferase==		==Structure and mechanisim of core 2 beta1,6-n-acetylglucosaminyltransferase: a Metal-ion independent gt-a glycosyltransferase==
	<StructureSection load='3otk' size='340' side='right' caption='[[3otk]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='3otk' size='340' side='right' caption='[[3otk]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3otk]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mus_musculus Mus musculus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OTK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OTK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3otk]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OTK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OTK FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HTO:HEPTANE-1,2,3-TRIOL'>HTO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HTO:HEPTANE-1,2,3-TRIOL'>HTO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Gcnt1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 ~~Mus musculus~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Gcnt1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-1,3-galactosyl-O-glycosyl-glycoprotein_beta-1,6-N-_acetylglucosaminyltransferase Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N- acetylglucosaminyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.102 2.4.1.102] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-1,3-galactosyl-O-glycosyl-glycoprotein_beta-1,6-N-_acetylglucosaminyltransferase Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N- acetylglucosaminyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.102 2.4.1.102] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3otk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3otk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3otk RCSB], [http://www.ebi.ac.uk/pdbsum/3otk PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3otk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3otk OCA], [http://pdbe.org/3otk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3otk RCSB], [http://www.ebi.ac.uk/pdbsum/3otk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3otk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3otk" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 27:		Line 29:
	</StructureSection>		</StructureSection>
	[[Category: Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N- acetylglucosaminyltransferase]]		[[Category: Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N- acetylglucosaminyltransferase]]
-	[[Category: ~~Mus musculus~~]]	+	[[Category: Lk3 transgenic mice]]
	[[Category: Pak, J E]]		[[Category: Pak, J E]]
	[[Category: Rini, J M]]		[[Category: Rini, J M]]

OCA at 20:57, 25 December 2014

OCA — Thu, 25 Dec 2014 20:57:51 GMT

←Older revision		Revision as of 20:57, 25 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3otk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3otk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3otk RCSB], [http://www.ebi.ac.uk/pdbsum/3otk PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3otk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3otk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3otk RCSB], [http://www.ebi.ac.uk/pdbsum/3otk PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/GCNT1_MOUSE GCNT1_MOUSE]] Forms critical branches in O-glycans.<ref>PMID:7983056</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 06:39, 19 December 2014

OCA — Fri, 19 Dec 2014 06:39:09 GMT

←Older revision		Revision as of 06:39, 19 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3otk\| PDB=3otk \| SCENE= }}~~	+	==Structure and mechanisim of core 2 beta1,6-n-acetylglucosaminyltransferase: a Metal-ion independent gt-a glycosyltransferase==
-	===Structure and mechanisim of core 2 beta1,6-n-acetylglucosaminyltransferase: a Metal-ion independent gt-a glycosyltransferase===	+	<StructureSection load='3otk' size='340' side='right' caption='[[3otk]], [[Resolution\|resolution]] 2.30Å' scene=''>
-	~~{{ABSTRACT_PUBMED_22056345}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3otk]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OTK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OTK FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HTO:HEPTANE-1,2,3-TRIOL'>HTO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Gcnt1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-1,3-galactosyl-O-glycosyl-glycoprotein_beta-1,6-N-_acetylglucosaminyltransferase Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N- acetylglucosaminyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.102 2.4.1.102] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3otk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3otk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3otk RCSB], [http://www.ebi.ac.uk/pdbsum/3otk PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Leukocyte-type core 2 beta1,6-N-acetylglucosaminyltransferase (C2GnT-L) is an inverting, metal-ion-independent glycosyltransferase that catalyzes the formation of mucin-type core 2 O-glycans. C2GnT-L belongs to the GT-A fold, yet it lacks the metal ion binding DXD motif characteristic of other nucleoside disphosphate GT-A fold glycosyltransferases. To shed light on the basis for its metal ion independence, we have solved the X-ray crystal structure (2.3 A resolution) of a mutant form of C2GnT-L (C217S) in complex with the nucleotide sugar product UDP and, using site-directed mutagenesis, examined the roles of R378 and K401 in both substrate binding and catalysis. The structure shows that C2GnT-L exists in an "open" conformation and a "closed" conformation and that, in the latter, R378 and K401 interact with the beta-phosphate moiety of the bound UDP. The two conformations are likely to be important in catalysis, but the conformational changes that lead to their interconversion do not resemble the nucleotide-sugar-mediated loop ordering observed in other GT-A glycosyltransferases. R378 and K401 were found to be important in substrate binding and/or catalysis, an observation consistent with the suggestion that they serve the same role played by metal ion in all of the other GT-A glycosyltransferases studied to date. Notably, R378 and K401 appear to function in a manner similar to that of the arginine and lysine residues contained in the RX(4-5)K motif found in the retaining GT-B glycosyltransferases.

-	~~==Function==~~	+	Structural and mechanistic characterization of leukocyte-type core 2 beta1,6-N-acetylglucosaminyltransferase: a metal-ion-independent GT-A glycosyltransferase.,Pak JE, Satkunarajah M, Seetharaman J, Rini JM J Mol Biol. 2011 Dec 16;414(5):798-811. Epub 2011 Oct 26. PMID:22056345<ref>PMID:22056345</ref>
-	~~[[http~~:~~//www~~.~~uniprot~~.~~org/uniprot/GCNT1_MOUSE GCNT1_MOUSE]] Forms critical branches in O~~-~~glycans~~.<ref>PMID:~~7983056~~</ref>	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[3otk]] is~~ a ~~4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Mus_musculus Mus musculus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3OTK OCA].~~	+	</div>

	==See Also==		==See Also==
	*[[Glycosyltransferase\|Glycosyltransferase]]		*[[Glycosyltransferase\|Glycosyltransferase]]
	*[[O-GlcNAc transferase\|O-GlcNAc transferase]]		*[[O-GlcNAc transferase\|O-GlcNAc transferase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:022056345</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N- acetylglucosaminyltransferase]]		[[Category: Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N- acetylglucosaminyltransferase]]
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
-	[[Category: Pak, J E.]]	+	[[Category: Pak, J E]]
-	[[Category: Rini, J M.]]	+	[[Category: Rini, J M]]
	[[Category: Glycosyltransferase]]		[[Category: Glycosyltransferase]]
	[[Category: Golgi]]		[[Category: Golgi]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 06:16, 2 October 2013

OCA — Wed, 02 Oct 2013 06:16:54 GMT

←Older revision		Revision as of 06:16, 2 October 2013
Line 1:		Line 1:
-	[[Image:3otk.png\|left\|200px]]
-
	{{STRUCTURE_3otk\| PDB=3otk \| SCENE= }}		{{STRUCTURE_3otk\| PDB=3otk \| SCENE= }}
-
	===Structure and mechanisim of core 2 beta1,6-n-acetylglucosaminyltransferase: a Metal-ion independent gt-a glycosyltransferase===		===Structure and mechanisim of core 2 beta1,6-n-acetylglucosaminyltransferase: a Metal-ion independent gt-a glycosyltransferase===
		+	{{ABSTRACT_PUBMED_22056345}}

		+	==Function==
		+	[[http://www.uniprot.org/uniprot/GCNT1_MOUSE GCNT1_MOUSE]] Forms critical branches in O-glycans.<ref>PMID:7983056</ref>

	==About this Structure==		==About this Structure==
Line 12:		Line 12:
	*[[Glycosyltransferase\|Glycosyltransferase]]		*[[Glycosyltransferase\|Glycosyltransferase]]
	*[[O-GlcNAc transferase\|O-GlcNAc transferase]]		*[[O-GlcNAc transferase\|O-GlcNAc transferase]]
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022056345</ref><references group="xtra"/><references/>
	[[Category: Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N- acetylglucosaminyltransferase]]		[[Category: Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N- acetylglucosaminyltransferase]]
	[[Category: Mus musculus]]		[[Category: Mus musculus]]

OCA at 21:33, 20 October 2012

OCA — Sat, 20 Oct 2012 21:33:45 GMT

←Older revision		Revision as of 21:33, 20 October 2012
Line 7:		Line 7:

	==About this Structure==		==About this Structure==
-	[[3otk]] is a 4 chain structure ~~of [[Glycosyltransferase]] and [[O-GlcNAc transferase]]~~ with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OTK OCA].	+	[[3otk]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OTK OCA].

	==See Also==		==See Also==

OCA at 13:36, 27 July 2012

OCA — Fri, 27 Jul 2012 13:36:44 GMT

←Older revision		Revision as of 13:36, 27 July 2012
Line 1:		Line 1:
-	[[Image:3otk.~~jpg~~\|left\|200px]]	+	[[Image:3otk.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3otk", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3otk\| PDB=3otk \| SCENE= }}		{{STRUCTURE_3otk\| PDB=3otk \| SCENE= }}

Line 13:		Line 7:

	==About this Structure==		==About this Structure==
-	[[3otk]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OTK OCA].	+	[[3otk]] is a 4 chain structure of [[Glycosyltransferase]] and [[O-GlcNAc transferase]] with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OTK OCA].
		+
		+	==See Also==
		+	*[[Glycosyltransferase\|Glycosyltransferase]]
		+	*[[O-GlcNAc transferase\|O-GlcNAc transferase]]
	[[Category: Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N- acetylglucosaminyltransferase]]		[[Category: Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N- acetylglucosaminyltransferase]]
	[[Category: Mus musculus]]		[[Category: Mus musculus]]

OCA at 06:32, 14 September 2011

OCA — Wed, 14 Sep 2011 06:32:27 GMT

←Older revision		Revision as of 06:32, 14 September 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3otk.jpg\|left\|200px]]

-	The ~~entry 3otk~~ is ~~ON HOLD~~ ~~until Sep 12 2012~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3otk", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3otk\| PDB=3otk \| SCENE= }}

-	~~Authors~~: ~~Pak, J.E., Rini, J.M.~~	+	===Structure and mechanisim of core 2 beta1,6-n-acetylglucosaminyltransferase: a Metal-ion independent gt-a glycosyltransferase===

-	~~Description~~: X-~~ray crystal structure of murine Core 2 b1~~,6-N-acetylglucosaminyltransferase ~~(C2GnT-L) in complex with UDP~~	+
		+	==About this Structure==
		+	[[3otk]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OTK OCA].
		+	[[Category: Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N- acetylglucosaminyltransferase]]
		+	[[Category: Mus musculus]]
		+	[[Category: Pak, J E.]]
		+	[[Category: Rini, J M.]]
		+	[[Category: Glycosyltransferase]]
		+	[[Category: Golgi]]
		+	[[Category: Transferase]]

OCA: Protected "3otk" [edit=sysop:move=sysop]

OCA — Wed, 31 Aug 2011 06:13:00 GMT

Protected "3otk" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:13, 31 August 2011