3ouv - Revision history

OCA at 11:19, 21 February 2024

OCA — Wed, 21 Feb 2024 11:19:00 GMT

←Older revision		Revision as of 11:19, 21 February 2024
Line 3:		Line 3:
	<StructureSection load='3ouv' size='340' side='right'caption='[[3ouv]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='3ouv' size='340' side='right'caption='[[3ouv]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3ouv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Mycta Mycta~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OUV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OUV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3ouv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Ra Mycobacterium tuberculosis H37Ra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OUV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OUV FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=MSE:SELENOMETHIONINE'>MSE</scene~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.004Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~pknB, MRA_0016 ([https~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=419947 MYCTA])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ouv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ouv OCA], [https://pdbe.org/3ouv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ouv RCSB], [https://www.ebi.ac.uk/pdbsum/3ouv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ouv ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ouv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ouv OCA], [https://pdbe.org/3ouv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ouv RCSB], [https://www.ebi.ac.uk/pdbsum/3ouv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ouv ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/A5TY84_MYCTA A5TY84_MYCTA]
-	Monitoring the environment with serine/threonine protein kinases is critical for growth and survival of Mycobacterium tuberculosis, a devastating human pathogen. Protein Kinase B (PknB) is a trans-membrane serine/threonine protein kinase that acts as an essential regulator of mycobacterial growth and division. The PknB extracellular domain (ECD) consists of four repeats homologous to penicillin-binding protein and serine/threonine kinase associated (PASTA) domains, and binds fragments of peptidoglycan. These properties suggest that PknB activity is modulated by ECD binding to peptidoglycan substructures, however the molecular mechanisms underpinning PknB regulation remain unclear. In this study, we report structural and genetic characterization of the PknB ECD. We determined the crystal structures of overlapping ECD fragments at near atomic resolution, built a model of the full ECD, and discovered a region on the C-terminal PASTA domain that has the properties of a ligand-binding site. Hydrophobic interaction between this surface and a bound molecule of citrate was observed in a crystal structure. Our genetic analyses in Mycobacterium tuberculosis showed that nonfunctional alleles were produced either by deletion of any of single PASTA domain or by mutation of individual conserved residues lining the putative ligand-binding surface of the C-terminal PASTA repeat. These results define two distinct structural features necessary for PknB signal transduction, a fully extended ECD and a conserved, membrane-distal putative ligand-binding site.	+
-		+
-	Structural and genetic analyses of the Mycobacterium tuberculosis Protein Kinase B sensor domain identify a potential ligand binding site.,Prigozhin DM, Papavinasasundaram KG, Baer CE, Murphy KC, Moskaleva A, Chen TY, Alber T, Sassetti CM J Biol Chem. 2016 Sep 6. pii: ~~jbc.M116.731760. PMID:27601474<ref>PMID:27601474<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®~~/~~PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 3ouv" style="background-color:#fffaf0;"><~~/~~div>~~	+

	==See Also==		==See Also==
	*[[Serine/threonine protein kinase 3D structures\|Serine/threonine protein kinase 3D structures]]		*[[Serine/threonine protein kinase 3D structures\|Serine/threonine protein kinase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Mycta~~]]	+	[[Category: Mycobacterium tuberculosis H37Ra]]
-	[[Category: Alber, T]]	+	[[Category: Alber T]]
-	[[Category: Chen~~, T Y~~]]	+	[[Category: Chen TY]]
-	[[Category: Prigozhin~~, D M]]~~	+	[[Category: Prigozhin DM]]
-	~~[[Category: Protein-ligand interaction]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 10:58, 18 May 2022

OCA — Wed, 18 May 2022 10:58:15 GMT

←Older revision		Revision as of 10:58, 18 May 2022
Line 1:		Line 1:

	==SeMet Derivative of L512M mutant of PASTA domain 3 of Mycobacterium tuberculosis PknB==		==SeMet Derivative of L512M mutant of PASTA domain 3 of Mycobacterium tuberculosis PknB==
-	<StructureSection load='3ouv' size='340' side='right' caption='[[3ouv]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='3ouv' size='340' side='right'caption='[[3ouv]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3ouv]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Mycta Mycta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OUV OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3OUV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3ouv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycta Mycta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OUV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OUV FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pknB, MRA_0016 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=419947 MYCTA])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pknB, MRA_0016 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=419947 MYCTA])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3ouv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ouv OCA], [~~http~~://pdbe.org/3ouv PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3ouv RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3ouv PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3ouv ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ouv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ouv OCA], [https://pdbe.org/3ouv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ouv RCSB], [https://www.ebi.ac.uk/pdbsum/3ouv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ouv ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 19:		Line 19:

	==See Also==		==See Also==
-	*[[Serine/threonine protein kinase\|Serine/threonine protein kinase]]	+	*[[Serine/threonine protein kinase 3D structures\|Serine/threonine protein kinase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Mycta]]		[[Category: Mycta]]
	[[Category: Alber, T]]		[[Category: Alber, T]]

OCA at 10:48, 13 February 2019

OCA — Wed, 13 Feb 2019 10:48:00 GMT

←Older revision		Revision as of 10:48, 13 February 2019
Line 3:		Line 3:
	<StructureSection load='3ouv' size='340' side='right' caption='[[3ouv]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='3ouv' size='340' side='right' caption='[[3ouv]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3ouv]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OUV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OUV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3ouv]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycta Mycta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OUV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OUV FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pknB, MRA_0016 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=419947 MYCTA])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ouv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ouv OCA], [http://pdbe.org/3ouv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ouv RCSB], [http://www.ebi.ac.uk/pdbsum/3ouv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ouv ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ouv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ouv OCA], [http://pdbe.org/3ouv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ouv RCSB], [http://www.ebi.ac.uk/pdbsum/3ouv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ouv ProSAT]</span></td></tr>
	</table>		</table>
Line 23:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Mycta]]
	[[Category: Alber, T]]		[[Category: Alber, T]]
	[[Category: Chen, T Y]]		[[Category: Chen, T Y]]

OCA at 11:32, 15 December 2016

OCA — Thu, 15 Dec 2016 11:32:53 GMT

←Older revision		Revision as of 11:32, 15 December 2016
Line 3:		Line 3:
	<StructureSection load='3ouv' size='340' side='right' caption='[[3ouv]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='3ouv' size='340' side='right' caption='[[3ouv]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3ouv]] is a 1 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Mycta Mycta]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OUV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OUV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3ouv]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OUV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OUV FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pknB, MRA_0016 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=419947 MYCTA])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ouv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ouv OCA], [http://pdbe.org/3ouv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ouv RCSB], [http://www.ebi.ac.uk/pdbsum/3ouv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ouv ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ouv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ouv OCA], [http://pdbe.org/3ouv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ouv RCSB], [http://www.ebi.ac.uk/pdbsum/3ouv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ouv ProSAT]</span></td></tr>
	</table>		</table>
Line 24:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Mycta]]
	[[Category: Alber, T]]		[[Category: Alber, T]]
	[[Category: Chen, T Y]]		[[Category: Chen, T Y]]

OCA at 08:17, 14 September 2016

OCA — Wed, 14 Sep 2016 08:17:09 GMT

←Older revision		Revision as of 08:17, 14 September 2016
Line 1:		Line 1:
		+
	==SeMet Derivative of L512M mutant of PASTA domain 3 of Mycobacterium tuberculosis PknB==		==SeMet Derivative of L512M mutant of PASTA domain 3 of Mycobacterium tuberculosis PknB==
	<StructureSection load='3ouv' size='340' side='right' caption='[[3ouv]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='3ouv' size='340' side='right' caption='[[3ouv]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3ouv]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mycobacterium_tuberculosis_h37ra Mycobacterium tuberculosis h37ra~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OUV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OUV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3ouv]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycta Mycta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OUV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OUV FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pknB, MRA_0016 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=419947 ~~Mycobacterium tuberculosis H37Ra~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pknB, MRA_0016 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=419947 MYCTA])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ouv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ouv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ouv RCSB], [http://www.ebi.ac.uk/pdbsum/3ouv PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ouv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ouv OCA], [http://pdbe.org/3ouv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ouv RCSB], [http://www.ebi.ac.uk/pdbsum/3ouv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ouv ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Monitoring the environment with serine/threonine protein kinases is critical for growth and survival of Mycobacterium tuberculosis, a devastating human pathogen. Protein Kinase B (PknB) is a trans-membrane serine/threonine protein kinase that acts as an essential regulator of mycobacterial growth and division. The PknB extracellular domain (ECD) consists of four repeats homologous to penicillin-binding protein and serine/threonine kinase associated (PASTA) domains, and binds fragments of peptidoglycan. These properties suggest that PknB activity is modulated by ECD binding to peptidoglycan substructures, however the molecular mechanisms underpinning PknB regulation remain unclear. In this study, we report structural and genetic characterization of the PknB ECD. We determined the crystal structures of overlapping ECD fragments at near atomic resolution, built a model of the full ECD, and discovered a region on the C-terminal PASTA domain that has the properties of a ligand-binding site. Hydrophobic interaction between this surface and a bound molecule of citrate was observed in a crystal structure. Our genetic analyses in Mycobacterium tuberculosis showed that nonfunctional alleles were produced either by deletion of any of single PASTA domain or by mutation of individual conserved residues lining the putative ligand-binding surface of the C-terminal PASTA repeat. These results define two distinct structural features necessary for PknB signal transduction, a fully extended ECD and a conserved, membrane-distal putative ligand-binding site.
		+
		+	Structural and genetic analyses of the Mycobacterium tuberculosis Protein Kinase B sensor domain identify a potential ligand binding site.,Prigozhin DM, Papavinasasundaram KG, Baer CE, Murphy KC, Moskaleva A, Chen TY, Alber T, Sassetti CM J Biol Chem. 2016 Sep 6. pii: jbc.M116.731760. PMID:27601474<ref>PMID:27601474</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 3ouv" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Serine/threonine protein kinase\|Serine/threonine protein kinase]]		*[[Serine/threonine protein kinase\|Serine/threonine protein kinase]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Mycobacterium tuberculosis h37ra~~]]	+	[[Category: Mycta]]
	[[Category: Alber, T]]		[[Category: Alber, T]]
	[[Category: Chen, T Y]]		[[Category: Chen, T Y]]

OCA at 13:38, 9 December 2014

OCA — Tue, 09 Dec 2014 13:38:19 GMT

←Older revision		Revision as of 13:38, 9 December 2014
Line 1:		Line 1:
-	~~[[Image:3ouv.png\|left\|200px]]~~	+	==SeMet Derivative of L512M mutant of PASTA domain 3 of Mycobacterium tuberculosis PknB==
-		+	<StructureSection load='3ouv' size='340' side='right' caption='[[3ouv]], [[Resolution\|resolution]] 2.00Å' scene=''>
-	~~{{STRUCTURE_3ouv\| PDB=3ouv \| SCENE= }}~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[3ouv]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_h37ra Mycobacterium tuberculosis h37ra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OUV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OUV FirstGlance]. <br>
-	===SeMet Derivative of L512M mutant of PASTA domain 3 of Mycobacterium tuberculosis PknB===	+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pknB, MRA_0016 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=419947 Mycobacterium tuberculosis H37Ra])</td></tr>
-		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ouv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ouv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ouv RCSB], [http://www.ebi.ac.uk/pdbsum/3ouv PDBsum]</span></td></tr>
-	==~~About this Structure~~==	+	</table>
-	[[3ouv]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_h37ra Mycobacterium tuberculosis h37ra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OUV OCA].	+

	==See Also==		==See Also==
	*[[Serine/threonine protein kinase\|Serine/threonine protein kinase]]		*[[Serine/threonine protein kinase\|Serine/threonine protein kinase]]
		+	__TOC__
		+	</StructureSection>
	[[Category: Mycobacterium tuberculosis h37ra]]		[[Category: Mycobacterium tuberculosis h37ra]]
-	[[Category: Alber, T.]]	+	[[Category: Alber, T]]
-	[[Category: Chen, T Y.]]	+	[[Category: Chen, T Y]]
-	[[Category: Prigozhin, D M.]]	+	[[Category: Prigozhin, D M]]
	[[Category: Protein-ligand interaction]]		[[Category: Protein-ligand interaction]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 17:35, 20 October 2012

OCA — Sat, 20 Oct 2012 17:35:47 GMT

←Older revision		Revision as of 17:35, 20 October 2012
Line 7:		Line 7:

	==About this Structure==		==About this Structure==
-	[[3ouv]] is a 1 chain structure ~~of [[Serine/threonine protein kinase]]~~ with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_h37ra Mycobacterium tuberculosis h37ra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OUV OCA].	+	[[3ouv]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_h37ra Mycobacterium tuberculosis h37ra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OUV OCA].

	==See Also==		==See Also==

OCA at 01:25, 27 July 2012

OCA — Fri, 27 Jul 2012 01:25:53 GMT

←Older revision		Revision as of 01:25, 27 July 2012
Line 1:		Line 1:
-	[[Image:3ouv.~~jpg~~\|left\|200px]]	+	[[Image:3ouv.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3ouv", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3ouv\| PDB=3ouv \| SCENE= }}		{{STRUCTURE_3ouv\| PDB=3ouv \| SCENE= }}

Line 13:		Line 7:

	==About this Structure==		==About this Structure==
-	[[3ouv]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_h37ra Mycobacterium tuberculosis h37ra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OUV OCA].	+	[[3ouv]] is a 1 chain structure of [[Serine/threonine protein kinase]] with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_h37ra Mycobacterium tuberculosis h37ra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OUV OCA].
		+
		+	==See Also==
		+	*[[Serine/threonine protein kinase\|Serine/threonine protein kinase]]
	[[Category: Mycobacterium tuberculosis h37ra]]		[[Category: Mycobacterium tuberculosis h37ra]]
	[[Category: Alber, T.]]		[[Category: Alber, T.]]

OCA at 06:06, 21 September 2011

OCA — Wed, 21 Sep 2011 06:06:10 GMT

←Older revision		Revision as of 06:06, 21 September 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3ouv.jpg\|left\|200px]]

-	The ~~entry 3ouv~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3ouv", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3ouv\| PDB=3ouv \| SCENE= }}

-	~~Authors: Prigozhin, D.~~	+	===SeMet Derivative of L512M mutant of PASTA domain 3 of Mycobacterium tuberculosis PknB===

-	~~Description~~: ~~SeMet Derivative of Mycobacterial Extracellular Domain~~.	+
		+	==About this Structure==
		+	[[3ouv]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_h37ra Mycobacterium tuberculosis h37ra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OUV OCA].
		+	[[Category: Mycobacterium tuberculosis h37ra]]
		+	[[Category: Alber, T.]]
		+	[[Category: Chen, T Y.]]
		+	[[Category: Prigozhin, D M.]]
		+	[[Category: Protein-ligand interaction]]
		+	[[Category: Transferase]]

OCA: Protected "3ouv" [edit=sysop:move=sysop]

OCA — Wed, 29 Dec 2010 18:12:21 GMT

Protected "3ouv" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:12, 29 December 2010