3ozb - Revision history

OCA at 10:37, 21 February 2024

OCA — Wed, 21 Feb 2024 10:37:37 GMT

←Older revision		Revision as of 10:37, 21 February 2024
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	<StructureSection load='3ozb' size='340' side='right'caption='[[3ozb]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='3ozb' size='340' side='right'caption='[[3ozb]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3ozb]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Pseae Pseae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OZB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OZB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3ozb]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OZB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OZB FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=HPA:HYPOXANTHINE'>HPA</scene>~~, ~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.8Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>mtnP, PA14_25210 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~208964 PSEAE])</td~~><~~/tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HPA:HYPOXANTHINE'>HPA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>[https://en.wikipedia.org/wiki/S-methyl-5'-thioadenosine_phosphorylase S-methyl-5'-thioadenosine phosphorylase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.28 2.4.2.28] </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ozb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ozb OCA], [https://pdbe.org/3ozb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ozb RCSB], [https://www.ebi.ac.uk/pdbsum/3ozb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ozb ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ozb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ozb OCA], [https://pdbe.org/3ozb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ozb RCSB], [https://www.ebi.ac.uk/pdbsum/3ozb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ozb ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~A0A0H2ZBV8_PSEAB A0A0H2ZBV8_PSEAB]~~] Catalyzes the reversible phosphorylation of S-methyl-5'-thioinosine (MTI) to hypoxanthine and 5-methylthioribose-1-phosphate. Involved in the breakdown of S-methyl-5'-thioadenosine (MTA), a major by-product of polyamine biosynthesis. Catabolism of (MTA) occurs via deamination to MTI and phosphorolysis to hypoxanthine.[HAMAP-Rule:MF_01963]	+	[https://www.uniprot.org/uniprot/MTIP_PSEAE MTIP_PSEAE] Catalyzes the reversible phosphorylation of S-methyl-5'-thioinosine (MTI) to hypoxanthine and 5-methylthioribose-1-phosphate. Involved in the breakdown of S-methyl-5'-thioadenosine (MTA), a major by-product of polyamine biosynthesis. Catabolism of (MTA) occurs via deamination to MTI and phosphorolysis to hypoxanthine. Involved in quorum sensing.[HAMAP-Rule:MF_01963]<ref>PMID:21197954</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	The PA3004 gene of Pseudomonas aeruginosa PAO1 was originally annotated as a 5'-methylthioadenosine phosphorylase (MTAP). However, the PA3004 encoded protein uses 5'-methylthioinosine (MTI) as a preferred substrate and represents the only known example of a specific MTI phosphorylase (MTIP). MTIP does not utilize 5'-methylthioadenosine (MTA). Inosine is a weak substrate with a k(cat)/K(m) value 290-fold less than MTI and is the second best substrate identified. The crystal structure of P. aeruginosa MTIP (PaMTIP) in complex with hypoxanthine was determined to 2.8 A resolution and revealed a 3-fold symmetric homotrimer. The methylthioribose and phosphate binding regions of PaMTIP are similar to MTAPs, and the purine binding region is similar to that of purine nucleoside phosphorylases (PNPs). The catabolism of MTA in P. aeruginosa involves deamination to MTI and phosphorolysis to hypoxanthine (MTA --> MTI --> hypoxanthine). This pathway also exists in Plasmodium falciparum, where the purine nucleoside phosphorylase (PfPNP) acts on both inosine and MTI. Three tight-binding transition state analogue inhibitors of PaMTIP are identified with dissociation constants in the picomolar range. Inhibitor specificity suggests an early dissociative transition state for PaMTIP. Quorum sensing molecules are associated with MTA metabolism in bacterial pathogens suggesting PaMTIP as a potential therapeutic target.	+
-		+
-	Methylthioinosine phosphorylase from Pseudomonas aeruginosa. Structure and annotation of a novel enzyme in quorum sensing.,Guan R, Ho MC, Almo SC, Schramm VL Biochemistry. 2011 Feb 22;50(7):1247-54. Epub 2011 Jan 25. PMID:21197954<ref>PMID:21197954</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3ozb" style="background-color:#fffaf0;"></div~~>	+
	== References ==		== References ==
	<references/>		<references/>
Line 25:		Line 15:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Pseae]]~~	+	[[Category: Pseudomonas aeruginosa PAO1]]
-	~~[[Category: S-methyl-5'-thioadenosine phosphorylase~~]]	+	[[Category: Almo SC]]
-	[[Category: Almo~~, S C~~]]	+	[[Category: Guan R]]
-	[[Category: Guan, R]]	+	[[Category: Ho M]]
-	[[Category: Ho, M]]	+	[[Category: Schramm VL]]
-	[[Category: Schramm~~, V L]]~~	+
-	~~[[Category: 5'-methylthioinosine]]~~	+
-	~~[[Category: Phosphorylase]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 11:04, 18 May 2022

OCA — Wed, 18 May 2022 11:04:39 GMT

←Older revision		Revision as of 11:04, 18 May 2022
Line 1:		Line 1:

	==Crystal Structure of 5'-methylthioinosine phosphorylase from Psedomonas aeruginosa in complex with hypoxanthine==		==Crystal Structure of 5'-methylthioinosine phosphorylase from Psedomonas aeruginosa in complex with hypoxanthine==
-	<StructureSection load='3ozb' size='340' side='right' caption='[[3ozb]], [[Resolution\|resolution]] 2.80Å' scene=''>	+	<StructureSection load='3ozb' size='340' side='right'caption='[[3ozb]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3ozb]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OZB OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3OZB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3ozb]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OZB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OZB FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HPA:HYPOXANTHINE'>HPA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HPA:HYPOXANTHINE'>HPA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mtnP, PA14_25210 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mtnP, PA14_25210 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/S-methyl-5'-thioadenosine_phosphorylase S-methyl-5'-thioadenosine phosphorylase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.28 2.4.2.28] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/S-methyl-5'-thioadenosine_phosphorylase S-methyl-5'-thioadenosine phosphorylase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.28 2.4.2.28] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3ozb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ozb OCA], [~~http~~://pdbe.org/3ozb PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3ozb RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3ozb PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3ozb ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ozb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ozb OCA], [https://pdbe.org/3ozb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ozb RCSB], [https://www.ebi.ac.uk/pdbsum/3ozb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ozb ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/A0A0H2ZBV8_PSEAB A0A0H2ZBV8_PSEAB]] Catalyzes the reversible phosphorylation of S-methyl-5'-thioinosine (MTI) to hypoxanthine and 5-methylthioribose-1-phosphate. Involved in the breakdown of S-methyl-5'-thioadenosine (MTA), a major by-product of polyamine biosynthesis. Catabolism of (MTA) occurs via deamination to MTI and phosphorolysis to hypoxanthine.[HAMAP-Rule:MF_01963]	+	[[https://www.uniprot.org/uniprot/A0A0H2ZBV8_PSEAB A0A0H2ZBV8_PSEAB]] Catalyzes the reversible phosphorylation of S-methyl-5'-thioinosine (MTI) to hypoxanthine and 5-methylthioribose-1-phosphate. Involved in the breakdown of S-methyl-5'-thioadenosine (MTA), a major by-product of polyamine biosynthesis. Catabolism of (MTA) occurs via deamination to MTI and phosphorolysis to hypoxanthine.[HAMAP-Rule:MF_01963]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Pseae]]		[[Category: Pseae]]
	[[Category: S-methyl-5'-thioadenosine phosphorylase]]		[[Category: S-methyl-5'-thioadenosine phosphorylase]]

OCA at 08:52, 8 November 2017

OCA — Wed, 08 Nov 2017 08:52:28 GMT

←Older revision		Revision as of 08:52, 8 November 2017
Line 9:		Line 9:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ozb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ozb OCA], [http://pdbe.org/3ozb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ozb RCSB], [http://www.ebi.ac.uk/pdbsum/3ozb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ozb ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ozb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ozb OCA], [http://pdbe.org/3ozb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ozb RCSB], [http://www.ebi.ac.uk/pdbsum/3ozb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ozb ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/A0A0H2ZBV8_PSEAB A0A0H2ZBV8_PSEAB]] Catalyzes the reversible phosphorylation of S-methyl-5'-thioinosine (MTI) to hypoxanthine and 5-methylthioribose-1-phosphate. Involved in the breakdown of S-methyl-5'-thioadenosine (MTA), a major by-product of polyamine biosynthesis. Catabolism of (MTA) occurs via deamination to MTI and phosphorolysis to hypoxanthine.[HAMAP-Rule:MF_01963]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 08:46, 11 August 2016

OCA — Thu, 11 Aug 2016 08:46:09 GMT

←Older revision		Revision as of 08:46, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal Structure of 5'-methylthioinosine phosphorylase from Psedomonas aeruginosa in complex with hypoxanthine==		==Crystal Structure of 5'-methylthioinosine phosphorylase from Psedomonas aeruginosa in complex with hypoxanthine==
	<StructureSection load='3ozb' size='340' side='right' caption='[[3ozb]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='3ozb' size='340' side='right' caption='[[3ozb]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3ozb]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Pseudomonas_aeruginosa Pseudomonas aeruginosa~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OZB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OZB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3ozb]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OZB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OZB FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HPA:HYPOXANTHINE'>HPA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HPA:HYPOXANTHINE'>HPA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mtnP, PA14_25210 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~287 Pseudomonas aeruginosa~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mtnP, PA14_25210 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/S-methyl-5'-thioadenosine_phosphorylase S-methyl-5'-thioadenosine phosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.28 2.4.2.28] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/S-methyl-5'-thioadenosine_phosphorylase S-methyl-5'-thioadenosine phosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.28 2.4.2.28] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ozb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ozb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ozb RCSB], [http://www.ebi.ac.uk/pdbsum/3ozb PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ozb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ozb OCA], [http://pdbe.org/3ozb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ozb RCSB], [http://www.ebi.ac.uk/pdbsum/3ozb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ozb ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3ozb" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Pseudomonas aeruginosa~~]]	+	[[Category: Pseae]]
	[[Category: S-methyl-5'-thioadenosine phosphorylase]]		[[Category: S-methyl-5'-thioadenosine phosphorylase]]
	[[Category: Almo, S C]]		[[Category: Almo, S C]]

OCA at 13:26, 6 November 2014

OCA — Thu, 06 Nov 2014 13:26:51 GMT

←Older revision		Revision as of 13:26, 6 November 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3ozb]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OZB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OZB FirstGlance]. <br>		<table><tr><td colspan='2'>[[3ozb]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OZB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OZB FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HPA:HYPOXANTHINE'>HPA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HPA:HYPOXANTHINE'>HPA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mtnP, PA14_25210 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 Pseudomonas aeruginosa])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mtnP, PA14_25210 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 Pseudomonas aeruginosa])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/S-methyl-5'-thioadenosine_phosphorylase S-methyl-5'-thioadenosine phosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.28 2.4.2.28] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/S-methyl-5'-thioadenosine_phosphorylase S-methyl-5'-thioadenosine phosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.28 2.4.2.28] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ozb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ozb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ozb RCSB], [http://www.ebi.ac.uk/pdbsum/3ozb PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ozb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ozb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ozb RCSB], [http://www.ebi.ac.uk/pdbsum/3ozb PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 14:		Line 14:
	Methylthioinosine phosphorylase from Pseudomonas aeruginosa. Structure and annotation of a novel enzyme in quorum sensing.,Guan R, Ho MC, Almo SC, Schramm VL Biochemistry. 2011 Feb 22;50(7):1247-54. Epub 2011 Jan 25. PMID:21197954<ref>PMID:21197954</ref>		Methylthioinosine phosphorylase from Pseudomonas aeruginosa. Structure and annotation of a novel enzyme in quorum sensing.,Guan R, Ho MC, Almo SC, Schramm VL Biochemistry. 2011 Feb 22;50(7):1247-54. Epub 2011 Jan 25. PMID:21197954<ref>PMID:21197954</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 22:		Line 22:
	[[Category: Pseudomonas aeruginosa]]		[[Category: Pseudomonas aeruginosa]]
	[[Category: S-methyl-5'-thioadenosine phosphorylase]]		[[Category: S-methyl-5'-thioadenosine phosphorylase]]
-	[[Category: Almo, S C.]]	+	[[Category: Almo, S C]]
-	[[Category: Guan, R.]]	+	[[Category: Guan, R]]
-	[[Category: Ho, M.]]	+	[[Category: Ho, M]]
-	[[Category: Schramm, V L.]]	+	[[Category: Schramm, V L]]
	[[Category: 5'-methylthioinosine]]		[[Category: 5'-methylthioinosine]]
	[[Category: Phosphorylase]]		[[Category: Phosphorylase]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 10:35, 28 May 2014

OCA — Wed, 28 May 2014 10:35:37 GMT

←Older revision		Revision as of 10:35, 28 May 2014
Line 1:		Line 1:
-	[[~~Image:~~3ozb.~~jpg~~\|~~left~~\|~~200px~~]]	+	==Crystal Structure of 5'-methylthioinosine phosphorylase from Psedomonas aeruginosa in complex with hypoxanthine==
		+	<StructureSection load='3ozb' size='340' side='right' caption='[[3ozb]], [[Resolution\|resolution]] 2.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3ozb]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OZB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OZB FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HPA:HYPOXANTHINE'>HPA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mtnP, PA14_25210 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 Pseudomonas aeruginosa])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/S-methyl-5'-thioadenosine_phosphorylase S-methyl-5'-thioadenosine phosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.28 2.4.2.28] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ozb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ozb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ozb RCSB], [http://www.ebi.ac.uk/pdbsum/3ozb PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The PA3004 gene of Pseudomonas aeruginosa PAO1 was originally annotated as a 5'-methylthioadenosine phosphorylase (MTAP). However, the PA3004 encoded protein uses 5'-methylthioinosine (MTI) as a preferred substrate and represents the only known example of a specific MTI phosphorylase (MTIP). MTIP does not utilize 5'-methylthioadenosine (MTA). Inosine is a weak substrate with a k(cat)/K(m) value 290-fold less than MTI and is the second best substrate identified. The crystal structure of P. aeruginosa MTIP (PaMTIP) in complex with hypoxanthine was determined to 2.8 A resolution and revealed a 3-fold symmetric homotrimer. The methylthioribose and phosphate binding regions of PaMTIP are similar to MTAPs, and the purine binding region is similar to that of purine nucleoside phosphorylases (PNPs). The catabolism of MTA in P. aeruginosa involves deamination to MTI and phosphorolysis to hypoxanthine (MTA --> MTI --> hypoxanthine). This pathway also exists in Plasmodium falciparum, where the purine nucleoside phosphorylase (PfPNP) acts on both inosine and MTI. Three tight-binding transition state analogue inhibitors of PaMTIP are identified with dissociation constants in the picomolar range. Inhibitor specificity suggests an early dissociative transition state for PaMTIP. Quorum sensing molecules are associated with MTA metabolism in bacterial pathogens suggesting PaMTIP as a potential therapeutic target.

-	~~<!--~~	+	Methylthioinosine phosphorylase from Pseudomonas aeruginosa. Structure and annotation of a novel enzyme in quorum sensing.,Guan R, Ho MC, Almo SC, Schramm VL Biochemistry. 2011 Feb 22;50(7):1247-54. Epub 2011 Jan 25. PMID:21197954<ref>PMID:21197954</ref>
-	~~The line below this paragraph~~, ~~containing "STRUCTURE_3ozb"~~, ~~creates the "Structure Box" on the page~~.	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~	+
-	~~or leave the SCENE parameter empty for the default display.~~	+
-	-->	+
-	~~{{STRUCTURE_3ozb\| PDB=3ozb \| SCENE= }}~~	+

-	~~===Crystal Structure~~ of ~~5'-methylthioinosine phosphorylase from Psedomonas aeruginosa in complex with hypoxanthine===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_21197954}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 21197954 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_21197954}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3ozb]] is a 6 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OZB OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:021197954<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Pseudomonas aeruginosa]]		[[Category: Pseudomonas aeruginosa]]
	[[Category: S-methyl-5'-thioadenosine phosphorylase]]		[[Category: S-methyl-5'-thioadenosine phosphorylase]]

OCA: Protected "3ozb" [edit=sysop:move=sysop]

OCA — Wed, 10 Aug 2011 05:57:02 GMT

Protected "3ozb" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:57, 10 August 2011

OCA at 05:56, 10 August 2011

OCA — Wed, 10 Aug 2011 05:56:58 GMT

←Older revision		Revision as of 05:56, 10 August 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3ozb.jpg\|left\|200px]]

-	The ~~entry 3ozb~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3ozb", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3ozb\| PDB=3ozb \| SCENE= }}

-	~~Authors: Ho, M., Guan, R., Almo, S.C., Schramm, V.L.~~	+	===Crystal Structure of 5'-methylthioinosine phosphorylase from Psedomonas aeruginosa in complex with hypoxanthine===

-	Description: Crystal Structure of 5'-methylthioinosine phosphorylase from Psedomonas aeruginosa in complex with hypoxanthine

-	~~''Page seeded by~~ [http://oca.weizmann.ac.il/oca OCA ] ~~on Wed Nov 24 13~~:56:~~32 2010~~''	+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21197954}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21197954 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21197954}}
		+
		+	==About this Structure==
		+	[[3ozb]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OZB OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:021197954</ref><references group="xtra"/>
		+	[[Category: Pseudomonas aeruginosa]]
		+	[[Category: S-methyl-5'-thioadenosine phosphorylase]]
		+	[[Category: Almo, S C.]]
		+	[[Category: Guan, R.]]
		+	[[Category: Ho, M.]]
		+	[[Category: Schramm, V L.]]
		+	[[Category: 5'-methylthioinosine]]
		+	[[Category: Phosphorylase]]
		+	[[Category: Transferase]]

OCA at 10:45, 24 November 2010

OCA — Wed, 24 Nov 2010 10:45:01 GMT

←Older revision		Revision as of 10:45, 24 November 2010
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3ozb is ON HOLD	+	The entry 3ozb is ON HOLD until Paper Publication

	Authors: Ho, M., Guan, R., Almo, S.C., Schramm, V.L.		Authors: Ho, M., Guan, R., Almo, S.C., Schramm, V.L.
Line 7:		Line 7:
	Description: Crystal Structure of 5'-methylthioinosine phosphorylase from Psedomonas aeruginosa in complex with hypoxanthine		Description: Crystal Structure of 5'-methylthioinosine phosphorylase from Psedomonas aeruginosa in complex with hypoxanthine

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Oct 6 05~~:50:09 2010''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Nov 24 13:56:32 2010''

OCA: New page: '''Unreleased structure''' The entry 3ozb is ON HOLD Authors: Ho, M., Guan, R., Almo, S.C., Schramm, V.L. Description: Crystal Structure of 5'-methylthioinosine phosphorylase from Psed...

OCA — Wed, 06 Oct 2010 02:40:38 GMT

New page: '''Unreleased structure''' The entry 3ozb is ON HOLD Authors: Ho, M., Guan, R., Almo, S.C., Schramm, V.L. Description: Crystal Structure of 5'-methylthioinosine phosphorylase from Psed...

New page

'''Unreleased structure'''

The entry 3ozb is ON HOLD

Authors: Ho, M., Guan, R., Almo, S.C., Schramm, V.L.

Description: Crystal Structure of 5'-methylthioinosine phosphorylase from Psedomonas aeruginosa in complex with hypoxanthine

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct 6 05:50:09 2010''