3p1l - Revision history

OCA at 10:37, 21 February 2024

OCA — Wed, 21 Feb 2024 10:37:56 GMT

←Older revision		Revision as of 10:37, 21 February 2024
Line 3:		Line 3:
	<StructureSection load='3p1l' size='340' side='right'caption='[[3p1l]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='3p1l' size='340' side='right'caption='[[3p1l]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3p1l]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Ecoli Ecoli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P1L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3P1L FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3p1l]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P1L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3P1L FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=NA:SODIUM+ION'>NA</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.6Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~b2512, JW2496, yfgL ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=83333 ECOLI])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3p1l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p1l OCA], [https://pdbe.org/3p1l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3p1l RCSB], [https://www.ebi.ac.uk/pdbsum/3p1l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3p1l ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3p1l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p1l OCA], [https://pdbe.org/3p1l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3p1l RCSB], [https://www.ebi.ac.uk/pdbsum/3p1l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3p1l ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/BAMB_ECOLI BAMB_ECOLI]] Part of the outer membrane protein assembly complex, which is involved in assembly and insertion of beta-barrel proteins into the outer membrane. Nonessential member of the complex, which may orient the flexible periplasmic domain of BamA for interaction with other Bam components, chaperones and nascent outer membrane proteins.<ref>PMID:20378773</ref> <ref>PMID:21823654</ref> <ref>PMID:21586578</ref> <ref>PMID:21277859</ref>	+	[https://www.uniprot.org/uniprot/BAMB_ECOLI BAMB_ECOLI] Part of the outer membrane protein assembly complex, which is involved in assembly and insertion of beta-barrel proteins into the outer membrane. Nonessential member of the complex, which may orient the flexible periplasmic domain of BamA for interaction with other Bam components, chaperones and nascent outer membrane proteins.<ref>PMID:20378773</ref> <ref>PMID:21823654</ref> <ref>PMID:21586578</ref> <ref>PMID:21277859</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	In Gram-negative bacteria, the BAM (beta-barrel assembly machinery) complex catalyzes the essential process of assembling outer membrane proteins. The BAM complex in Escherichia coli consists of five proteins: one beta-barrel membrane protein, BamA, and four lipoproteins, BamB, BamC, BamD, and BamE. Despite their role in outer membrane protein biogenesis, there is currently a lack of functional and structural information on the lipoprotein components of the BAM complex. Here, we report the first crystal structure of BamB, the largest and most functionally characterized lipoprotein component of the BAM complex. The crystal structure shows that BamB has an eight-bladed beta-propeller structure, with four beta-strands making up each blade. Mapping onto the structure the residues previously shown to be important for BamA interaction reveals that these residues, despite being far apart in the amino acid sequence, are localized to form a continuous solvent-exposed surface on one side of the beta-propeller. Found on the same side of the beta-propeller is a cluster of residues conserved among BamB homologs. Interestingly, our structural comparison study suggests that other proteins with a BamB-like fold often participate in protein or ligand binding, and that the binding interface on these proteins is located on the surface that is topologically equivalent to where the conserved residues and the residues that are important for BamA interaction are found on BamB. Our structural and bioinformatic analyses, together with previous biochemical data, provide clues to where the BamA and possibly a substrate interaction interface may be located on BamB.	+
-		+
-	~~Crystal Structure of Escherichia coli BamB, a Lipoprotein Component of the beta-Barrel Assembly Machinery Complex.,Kim KH, Paetzel M J Mol Biol. 2010 Dec 17. PMID:21168416<ref>PMID:21168416</ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3p1l" style="background-color:#fffaf0;"></div~~>	+

	==See Also==		==See Also==
Line 26:		Line 17:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Ecoli~~]]	+	[[Category: Escherichia coli K-12]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Kim~~, K H~~]]	+	[[Category: Kim KH]]
-	[[Category: Paetzel, M]]	+	[[Category: Paetzel M]]
-	~~[[Category: Beta propeller]]~~	+
-	~~[[Category: Lipoprotein involved in beta-barrel assembly in e. coli]]~~	+
-	~~[[Category: Protein binding~~]]	+

OCA at 11:10, 18 May 2022

OCA — Wed, 18 May 2022 11:10:41 GMT

←Older revision		Revision as of 11:10, 18 May 2022
Line 1:		Line 1:

	==Crystal structure of Escherichia coli BamB, a lipoprotein component of the beta-barrel assembly machinery complex, native crystals.==		==Crystal structure of Escherichia coli BamB, a lipoprotein component of the beta-barrel assembly machinery complex, native crystals.==
-	<StructureSection load='3p1l' size='340' side='right' caption='[[3p1l]], [[Resolution\|resolution]] 2.60Å' scene=''>	+	<StructureSection load='3p1l' size='340' side='right'caption='[[3p1l]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3p1l]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P1L OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3P1L FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3p1l]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P1L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3P1L FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b2512, JW2496, yfgL ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b2512, JW2496, yfgL ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3p1l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p1l OCA], [~~http~~://pdbe.org/3p1l PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3p1l RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3p1l PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3p1l ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3p1l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p1l OCA], [https://pdbe.org/3p1l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3p1l RCSB], [https://www.ebi.ac.uk/pdbsum/3p1l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3p1l ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/BAMB_ECOLI BAMB_ECOLI]] Part of the outer membrane protein assembly complex, which is involved in assembly and insertion of beta-barrel proteins into the outer membrane. Nonessential member of the complex, which may orient the flexible periplasmic domain of BamA for interaction with other Bam components, chaperones and nascent outer membrane proteins.<ref>PMID:20378773</ref> <ref>PMID:21823654</ref> <ref>PMID:21586578</ref> <ref>PMID:21277859</ref>	+	[[https://www.uniprot.org/uniprot/BAMB_ECOLI BAMB_ECOLI]] Part of the outer membrane protein assembly complex, which is involved in assembly and insertion of beta-barrel proteins into the outer membrane. Nonessential member of the complex, which may orient the flexible periplasmic domain of BamA for interaction with other Bam components, chaperones and nascent outer membrane proteins.<ref>PMID:20378773</ref> <ref>PMID:21823654</ref> <ref>PMID:21586578</ref> <ref>PMID:21277859</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 3p1l" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3p1l" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Bam complex 3D structures\|Bam complex 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 24:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: Ecoli]]		[[Category: Ecoli]]
		+	[[Category: Large Structures]]
	[[Category: Kim, K H]]		[[Category: Kim, K H]]
	[[Category: Paetzel, M]]		[[Category: Paetzel, M]]

OCA at 08:53, 8 November 2017

OCA — Wed, 08 Nov 2017 08:53:27 GMT

←Older revision		Revision as of 08:53, 8 November 2017
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 3p1l" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3p1l" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Bam complex\|Bam complex]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:23, 5 August 2016

OCA — Fri, 05 Aug 2016 11:23:27 GMT

←Older revision		Revision as of 11:23, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of Escherichia coli BamB, a lipoprotein component of the beta-barrel assembly machinery complex, native crystals.==		==Crystal structure of Escherichia coli BamB, a lipoprotein component of the beta-barrel assembly machinery complex, native crystals.==
	<StructureSection load='3p1l' size='340' side='right' caption='[[3p1l]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='3p1l' size='340' side='right' caption='[[3p1l]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3p1l]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia coli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P1L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3P1L FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3p1l]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P1L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3P1L FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b2512, JW2496, yfgL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~562 Escherichia coli~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b2512, JW2496, yfgL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3p1l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p1l OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3p1l RCSB], [http://www.ebi.ac.uk/pdbsum/3p1l PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3p1l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p1l OCA], [http://pdbe.org/3p1l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3p1l RCSB], [http://www.ebi.ac.uk/pdbsum/3p1l PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3p1l ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/~~YFGL_ECOLI YFGL_ECOLI~~]] Part of the outer membrane protein assembly complex, which is involved in assembly and insertion of beta-barrel proteins into the outer membrane. Nonessential member of the complex, which may orient the flexible periplasmic domain of BamA for interaction with other Bam components, chaperones and nascent outer membrane proteins.<ref>PMID:20378773</ref> <ref>PMID:21823654</ref> <ref>PMID:21586578</ref> <ref>PMID:21277859</ref>	+	[[http://www.uniprot.org/uniprot/BAMB_ECOLI BAMB_ECOLI]] Part of the outer membrane protein assembly complex, which is involved in assembly and insertion of beta-barrel proteins into the outer membrane. Nonessential member of the complex, which may orient the flexible periplasmic domain of BamA for interaction with other Bam components, chaperones and nascent outer membrane proteins.<ref>PMID:20378773</ref> <ref>PMID:21823654</ref> <ref>PMID:21586578</ref> <ref>PMID:21277859</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3p1l" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Bam complex\|Bam complex]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia coli~~]]	+	[[Category: Ecoli]]
	[[Category: Kim, K H]]		[[Category: Kim, K H]]
	[[Category: Paetzel, M]]		[[Category: Paetzel, M]]

OCA at 01:59, 25 December 2014

OCA — Thu, 25 Dec 2014 01:59:28 GMT

←Older revision		Revision as of 01:59, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3p1l]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P1L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3P1L FirstGlance]. <br>		<table><tr><td colspan='2'>[[3p1l]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P1L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3P1L FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b2512, JW2496, yfgL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b2512, JW2496, yfgL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3p1l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p1l OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3p1l RCSB], [http://www.ebi.ac.uk/pdbsum/3p1l PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3p1l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p1l OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3p1l RCSB], [http://www.ebi.ac.uk/pdbsum/3p1l PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/YFGL_ECOLI YFGL_ECOLI]] Part of the outer membrane protein assembly complex, which is involved in assembly and insertion of beta-barrel proteins into the outer membrane. Nonessential member of the complex, which may orient the flexible periplasmic domain of BamA for interaction with other Bam components, chaperones and nascent outer membrane proteins.<ref>PMID:20378773</ref> <ref>PMID:21823654</ref> <ref>PMID:21586578</ref> <ref>PMID:21277859</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 13:		Line 15:
	Crystal Structure of Escherichia coli BamB, a Lipoprotein Component of the beta-Barrel Assembly Machinery Complex.,Kim KH, Paetzel M J Mol Biol. 2010 Dec 17. PMID:21168416<ref>PMID:21168416</ref>		Crystal Structure of Escherichia coli BamB, a Lipoprotein Component of the beta-Barrel Assembly Machinery Complex.,Kim KH, Paetzel M J Mol Biol. 2010 Dec 17. PMID:21168416<ref>PMID:21168416</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 20:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Kim, K H.]]	+	[[Category: Kim, K H]]
-	[[Category: Paetzel, M.]]	+	[[Category: Paetzel, M]]
	[[Category: Beta propeller]]		[[Category: Beta propeller]]
	[[Category: Lipoprotein involved in beta-barrel assembly in e. coli]]		[[Category: Lipoprotein involved in beta-barrel assembly in e. coli]]
	[[Category: Protein binding]]		[[Category: Protein binding]]

OCA at 10:31, 28 May 2014

OCA — Wed, 28 May 2014 10:31:23 GMT

←Older revision		Revision as of 10:31, 28 May 2014
Line 1:		Line 1:
-	[[~~Image:~~3p1l.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of Escherichia coli BamB, a lipoprotein component of the beta-barrel assembly machinery complex, native crystals.==
		+	<StructureSection load='3p1l' size='340' side='right' caption='[[3p1l]], [[Resolution\|resolution]] 2.60Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3p1l]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P1L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3P1L FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b2512, JW2496, yfgL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3p1l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p1l OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3p1l RCSB], [http://www.ebi.ac.uk/pdbsum/3p1l PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In Gram-negative bacteria, the BAM (beta-barrel assembly machinery) complex catalyzes the essential process of assembling outer membrane proteins. The BAM complex in Escherichia coli consists of five proteins: one beta-barrel membrane protein, BamA, and four lipoproteins, BamB, BamC, BamD, and BamE. Despite their role in outer membrane protein biogenesis, there is currently a lack of functional and structural information on the lipoprotein components of the BAM complex. Here, we report the first crystal structure of BamB, the largest and most functionally characterized lipoprotein component of the BAM complex. The crystal structure shows that BamB has an eight-bladed beta-propeller structure, with four beta-strands making up each blade. Mapping onto the structure the residues previously shown to be important for BamA interaction reveals that these residues, despite being far apart in the amino acid sequence, are localized to form a continuous solvent-exposed surface on one side of the beta-propeller. Found on the same side of the beta-propeller is a cluster of residues conserved among BamB homologs. Interestingly, our structural comparison study suggests that other proteins with a BamB-like fold often participate in protein or ligand binding, and that the binding interface on these proteins is located on the surface that is topologically equivalent to where the conserved residues and the residues that are important for BamA interaction are found on BamB. Our structural and bioinformatic analyses, together with previous biochemical data, provide clues to where the BamA and possibly a substrate interaction interface may be located on BamB.

-	~~<!--~~	+	Crystal Structure of Escherichia coli BamB, a Lipoprotein Component of the beta-Barrel Assembly Machinery Complex.,Kim KH, Paetzel M J Mol Biol. 2010 Dec 17. PMID:21168416<ref>PMID:21168416</ref>
-	~~The line below this paragraph~~, ~~containing "STRUCTURE_3p1l", creates~~ the ~~"Structure Box" on the page~~.	+
-	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded)~~,	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_3p1l\| PDB=3p1l \| SCENE= }}~~	+

-	~~===Crystal structure of Escherichia coli BamB~~, a ~~lipoprotein component~~ of the ~~beta-barrel assembly machinery complex, native crystals~~.~~===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_21168416}}, adds the Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 21168416 is the PubMed ID number.~~	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_21168416}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3p1l]] is a 1 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P1L OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:021168416<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Kim, K H.]]		[[Category: Kim, K H.]]

OCA at 08:25, 16 May 2012

OCA — Wed, 16 May 2012 08:25:58 GMT

←Older revision		Revision as of 08:25, 16 May 2012
Line 22:		Line 22:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~21168416~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:021168416</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Kim, K H.]]		[[Category: Kim, K H.]]
-	[[Category: Paetzel, M W.]]	+	[[Category: Paetzel, M.]]
		+	[[Category: Beta propeller]]
		+	[[Category: Lipoprotein involved in beta-barrel assembly in e. coli]]
		+	[[Category: Protein binding]]

OCA at 05:48, 9 March 2011

OCA — Wed, 09 Mar 2011 05:48:11 GMT

←Older revision		Revision as of 05:48, 9 March 2011
Line 26:		Line 26:
	[[Category: Kim, K H.]]		[[Category: Kim, K H.]]
	[[Category: Paetzel, M W.]]		[[Category: Paetzel, M W.]]
-	[[Category: Beta propeller]]
-	[[Category: Lipoprotein involved in beta-barrel assembly in e. coli]]
-	[[Category: Protein binding]]

OCA: Protected "3p1l" [edit=sysop:move=sysop]

OCA — Wed, 29 Dec 2010 17:57:18 GMT

Protected "3p1l" [edit=sysop:move=sysop]

←Older revision

Revision as of 17:57, 29 December 2010

OCA at 17:57, 29 December 2010

OCA — Wed, 29 Dec 2010 17:57:16 GMT

←Older revision		Revision as of 17:57, 29 December 2010
Line 1:		Line 1:
-	~~{{Seed}}~~	+	[[Image:3p1l.png\|left\|200px]]
-	[[Image:3p1l.~~jpg~~\|left\|200px]]	+

	<!--		<!--
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	===Crystal structure of Escherichia coli BamB, a lipoprotein component of the beta-barrel assembly machinery complex, native crystals.===		===Crystal structure of Escherichia coli BamB, a lipoprotein component of the beta-barrel assembly machinery complex, native crystals.===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21168416}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21168416 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21168416}}

	==About this Structure==		==About this Structure==
-	~~3P1L~~ is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P1L OCA].	+	[[3p1l]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P1L OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:21168416</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Kim, K H.]]		[[Category: Kim, K H.]]
Line 21:		Line 29:
	[[Category: Lipoprotein involved in beta-barrel assembly in e. coli]]		[[Category: Lipoprotein involved in beta-barrel assembly in e. coli]]
	[[Category: Protein binding]]		[[Category: Protein binding]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 22 09:58:14 2010''