3p23 - Revision history

OCA at 10:37, 21 February 2024

OCA — Wed, 21 Feb 2024 10:37:59 GMT

←Older revision		Revision as of 10:37, 21 February 2024
Line 3:		Line 3:
	<StructureSection load='3p23' size='340' side='right'caption='[[3p23]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='3p23' size='340' side='right'caption='[[3p23]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3p23]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P23 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3P23 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3p23]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P23 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3P23 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.7Å</td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ERN1, IRE1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3p23 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p23 OCA], [https://pdbe.org/3p23 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3p23 RCSB], [https://www.ebi.ac.uk/pdbsum/3p23 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3p23 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3p23 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p23 OCA], [https://pdbe.org/3p23 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3p23 RCSB], [https://www.ebi.ac.uk/pdbsum/3p23 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3p23 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/ERN1_HUMAN ERN1_HUMAN]] Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.<ref>PMID:9637683</ref> <ref>PMID:11175748</ref> <ref>PMID:12637535</ref> [UniProtKB:Q9EQY0]	+	[https://www.uniprot.org/uniprot/ERN1_HUMAN ERN1_HUMAN] Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.<ref>PMID:9637683</ref> <ref>PMID:11175748</ref> <ref>PMID:12637535</ref> [UniProtKB:Q9EQY0]
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Ire1 (Ern1) is an unusual transmembrane protein kinase essential for the endoplasmic reticulum (ER) unfolded protein response (UPR). Activation of Ire1 by association of its N-terminal ER luminal domains promotes autophosphorylation by its cytoplasmic kinase domain, leading to activation of the C-terminal ribonuclease domain, which splices Xbp1 mRNA generating an active Xbp1s transcriptional activator. We have determined the crystal structure of the cytoplasmic portion of dephosphorylated human Ire1alpha bound to ADP, revealing the 'phosphoryl-transfer' competent dimeric face-to-face complex, which precedes and is distinct from the back-to-back RNase 'active' conformation described for yeast Ire1. We show that the Xbp1-specific ribonuclease activity depends on autophosphorylation, and that ATP-competitive inhibitors staurosporin and sunitinib, which inhibit autophosphorylation in vitro, also inhibit Xbp1 splicing in vivo. Furthermore, we demonstrate that activated Ire1alpha is a competent protein kinase, able to phosphorylate a heterologous peptide substrate. These studies identify human Ire1alpha as a target for development of ATP-competitive inhibitors that will modulate the UPR in human cells, which has particular relevance for myeloma and other secretory malignancies.	+
-		+
-	Structure of the Ire1 autophosphorylation complex and implications for the unfolded protein response.,Ali MM, Bagratuni T, Davenport EL, Nowak PR, Silva-Santisteban MC, Hardcastle A, McAndrews C, Rowlands MG, Morgan GJ, Aherne W, Collins I, Davies FE, Pearl LH EMBO J. 2011 Feb 11. PMID:21317875<ref>PMID:21317875</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3p23" style="background-color:#fffaf0;"></div>~~	+

	==See Also==		==See Also==
Line 26:		Line 17:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Ali~~, M M.U~~]]	+	[[Category: Ali MMU]]
-	[[Category: Pearl~~, L H]]~~	+	[[Category: Pearl LH]]
-	~~[[Category: Atp binding ssrna binding]]~~	+
-	~~[[Category: Dephosphorylated]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Kinase and rnase function]]~~	+
-	~~[[Category: Kinase domain]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 11:11, 18 May 2022

OCA — Wed, 18 May 2022 11:11:23 GMT

←Older revision		Revision as of 11:11, 18 May 2022
Line 1:		Line 1:

	==Crystal structure of the Human kinase and RNase domains in complex with ADP==		==Crystal structure of the Human kinase and RNase domains in complex with ADP==
-	<StructureSection load='3p23' size='340' side='right' caption='[[3p23]], [[Resolution\|resolution]] 2.70Å' scene=''>	+	<StructureSection load='3p23' size='340' side='right'caption='[[3p23]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3p23]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P23 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3P23 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3p23]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P23 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3P23 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ERN1, IRE1 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ERN1, IRE1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3p23 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p23 OCA], [~~http~~://pdbe.org/3p23 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3p23 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3p23 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3p23 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3p23 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p23 OCA], [https://pdbe.org/3p23 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3p23 RCSB], [https://www.ebi.ac.uk/pdbsum/3p23 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3p23 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/ERN1_HUMAN ERN1_HUMAN]] Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.<ref>PMID:9637683</ref> <ref>PMID:11175748</ref> <ref>PMID:12637535</ref> [UniProtKB:Q9EQY0]	+	[[https://www.uniprot.org/uniprot/ERN1_HUMAN ERN1_HUMAN]] Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.<ref>PMID:9637683</ref> <ref>PMID:11175748</ref> <ref>PMID:12637535</ref> [UniProtKB:Q9EQY0]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 27:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Ali, M M.U]]		[[Category: Ali, M M.U]]
	[[Category: Pearl, L H]]		[[Category: Pearl, L H]]

OCA at 01:31, 5 August 2016

OCA — Fri, 05 Aug 2016 01:31:35 GMT

←Older revision		Revision as of 01:31, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of the Human kinase and RNase domains in complex with ADP==		==Crystal structure of the Human kinase and RNase domains in complex with ADP==
	<StructureSection load='3p23' size='340' side='right' caption='[[3p23]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='3p23' size='340' side='right' caption='[[3p23]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3p23]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P23 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3P23 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3p23]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P23 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3P23 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ERN1, IRE1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ERN1, IRE1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3p23 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p23 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3p23 RCSB], [http://www.ebi.ac.uk/pdbsum/3p23 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3p23 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p23 OCA], [http://pdbe.org/3p23 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3p23 RCSB], [http://www.ebi.ac.uk/pdbsum/3p23 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3p23 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3p23" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 24:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Ali, M M.U]]		[[Category: Ali, M M.U]]
	[[Category: Pearl, L H]]		[[Category: Pearl, L H]]

OCA at 21:37, 24 December 2014

OCA — Wed, 24 Dec 2014 21:37:47 GMT

←Older revision		Revision as of 21:37, 24 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3p23 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p23 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3p23 RCSB], [http://www.ebi.ac.uk/pdbsum/3p23 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3p23 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p23 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3p23 RCSB], [http://www.ebi.ac.uk/pdbsum/3p23 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ERN1_HUMAN ERN1_HUMAN]] Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.<ref>PMID:9637683</ref> <ref>PMID:11175748</ref> <ref>PMID:12637535</ref> [UniProtKB:Q9EQY0]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 06:40, 19 December 2014

OCA — Fri, 19 Dec 2014 06:40:51 GMT

←Older revision		Revision as of 06:40, 19 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3p23\| PDB=3p23 \| SCENE= }}~~	+	==Crystal structure of the Human kinase and RNase domains in complex with ADP==
-	===Crystal structure of the Human kinase and RNase domains in complex with ADP===	+	<StructureSection load='3p23' size='340' side='right' caption='[[3p23]], [[Resolution\|resolution]] 2.70Å' scene=''>
-	~~{{ABSTRACT_PUBMED_21317875}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3p23]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P23 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3P23 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ERN1, IRE1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3p23 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p23 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3p23 RCSB], [http://www.ebi.ac.uk/pdbsum/3p23 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Ire1 (Ern1) is an unusual transmembrane protein kinase essential for the endoplasmic reticulum (ER) unfolded protein response (UPR). Activation of Ire1 by association of its N-terminal ER luminal domains promotes autophosphorylation by its cytoplasmic kinase domain, leading to activation of the C-terminal ribonuclease domain, which splices Xbp1 mRNA generating an active Xbp1s transcriptional activator. We have determined the crystal structure of the cytoplasmic portion of dephosphorylated human Ire1alpha bound to ADP, revealing the 'phosphoryl-transfer' competent dimeric face-to-face complex, which precedes and is distinct from the back-to-back RNase 'active' conformation described for yeast Ire1. We show that the Xbp1-specific ribonuclease activity depends on autophosphorylation, and that ATP-competitive inhibitors staurosporin and sunitinib, which inhibit autophosphorylation in vitro, also inhibit Xbp1 splicing in vivo. Furthermore, we demonstrate that activated Ire1alpha is a competent protein kinase, able to phosphorylate a heterologous peptide substrate. These studies identify human Ire1alpha as a target for development of ATP-competitive inhibitors that will modulate the UPR in human cells, which has particular relevance for myeloma and other secretory malignancies.

-	~~==Function==~~	+	Structure of the Ire1 autophosphorylation complex and implications for the unfolded protein response.,Ali MM, Bagratuni T, Davenport EL, Nowak PR, Silva-Santisteban MC, Hardcastle A, McAndrews C, Rowlands MG, Morgan GJ, Aherne W, Collins I, Davies FE, Pearl LH EMBO J. 2011 Feb 11. PMID:21317875<ref>PMID:21317875</ref>
-	~~[[http://www.uniprot.org/uniprot/ERN1_HUMAN ERN1_HUMAN]] Senses unfolded proteins in the lumen~~ of the ~~endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent~~ unfolded-protein response ~~transcriptional activator and triggering growth arrest and apoptosis~~.~~<ref>~~PMID:~~9637683</ref>~~ <ref>PMID:~~11175748~~</ref> ~~<ref>PMID:12637535</ref> [UniProtKB:Q9EQY0]~~	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[3p23]] is~~ a ~~4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3P23 OCA].~~	+	</div>

	==See Also==		==See Also==
	*[[Ire1\|Ire1]]		*[[Ire1\|Ire1]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:021317875</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Ali, M M.U.]]	+	[[Category: Ali, M M.U]]
-	[[Category: Pearl, L H.]]	+	[[Category: Pearl, L H]]
	[[Category: Atp binding ssrna binding]]		[[Category: Atp binding ssrna binding]]
	[[Category: Dephosphorylated]]		[[Category: Dephosphorylated]]

OCA at 08:39, 21 August 2013

OCA — Wed, 21 Aug 2013 08:39:15 GMT

←Older revision		Revision as of 08:39, 21 August 2013
Line 1:		Line 1:
-	[[Image:3p23.png\|left\|200px]]
-
	{{STRUCTURE_3p23\| PDB=3p23 \| SCENE= }}		{{STRUCTURE_3p23\| PDB=3p23 \| SCENE= }}
-
	===Crystal structure of the Human kinase and RNase domains in complex with ADP===		===Crystal structure of the Human kinase and RNase domains in complex with ADP===
		+	{{ABSTRACT_PUBMED_21317875}}

-	~~{{ABSTRACT_PUBMED_21317875}}~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/ERN1_HUMAN ERN1_HUMAN]] Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.<ref>PMID:9637683</ref> <ref>PMID:11175748</ref> <ref>PMID:12637535</ref> [UniProtKB:Q9EQY0]

	==About this Structure==		==About this Structure==
Line 14:		Line 13:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:021317875</ref><references group="xtra"/>	+	<ref group="xtra">PMID:021317875</ref><references group="xtra"/><references/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Ali, M M.U.]]		[[Category: Ali, M M.U.]]

OCA at 09:23, 20 October 2012

OCA — Sat, 20 Oct 2012 09:23:00 GMT

←Older revision		Revision as of 09:23, 20 October 2012
Line 1:		Line 1:
	[[Image:3p23.png\|left\|200px]]		[[Image:3p23.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3p23", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3p23\| PDB=3p23 \| SCENE= }}		{{STRUCTURE_3p23\| PDB=3p23 \| SCENE= }}

	===Crystal structure of the Human kinase and RNase domains in complex with ADP===		===Crystal structure of the Human kinase and RNase domains in complex with ADP===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_21317875}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 21317875 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_21317875}}		{{ABSTRACT_PUBMED_21317875}}

	==About this Structure==		==About this Structure==
	[[3p23]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P23 OCA].		[[3p23]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P23 OCA].
		+
		+	==See Also==
		+	*[[Ire1\|Ire1]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~21317875~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:021317875</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Ali, M M.U.]]		[[Category: Ali, M M.U.]]
	[[Category: Pearl, L H.]]		[[Category: Pearl, L H.]]
		+	[[Category: Atp binding ssrna binding]]
		+	[[Category: Dephosphorylated]]
		+	[[Category: Hydrolase]]
		+	[[Category: Kinase and rnase function]]
		+	[[Category: Kinase domain]]
		+	[[Category: Transferase]]

OCA at 07:31, 16 March 2011

OCA — Wed, 16 Mar 2011 07:31:24 GMT

←Older revision		Revision as of 07:31, 16 March 2011
Line 1:		Line 1:
-	[[Image:3p23.~~jpg~~\|left\|200px]]	+	[[Image:3p23.png\|left\|200px]]

	<!--		<!--
Line 19:		Line 19:

	==About this Structure==		==About this Structure==
-	[[3p23]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Thermomicrobium_roseum Thermomicrobium roseum~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P23 OCA].	+	[[3p23]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P23 OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:21317875</ref><references group="xtra"/>		<ref group="xtra">PMID:21317875</ref><references group="xtra"/>
-	[[Category: ~~Thermomicrobium roseum~~]]	+	[[Category: Homo sapiens]]
	[[Category: Ali, M M.U.]]		[[Category: Ali, M M.U.]]
	[[Category: Pearl, L H.]]		[[Category: Pearl, L H.]]

OCA: Protected "3p23" [edit=sysop:move=sysop]

OCA — Wed, 23 Feb 2011 09:52:33 GMT

Protected "3p23" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:52, 23 February 2011

OCA at 09:52, 23 February 2011

OCA — Wed, 23 Feb 2011 09:52:30 GMT

←Older revision		Revision as of 09:52, 23 February 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3p23.jpg\|left\|200px]]

-	The ~~entry 3p23~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3p23", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3p23\| PDB=3p23 \| SCENE= }}

-	~~Authors: Ali, M.M.U., Pearl, L.H.~~	+	===Crystal structure of the Human kinase and RNase domains in complex with ADP===

-	Description: Crystal structure of the Human kinase and RNase domains in complex with ADP

-	~~''Page seeded by~~ [http://oca.weizmann.ac.il/oca OCA ] ~~on Wed Oct 13 10~~:33:~~03 2010''~~	+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21317875}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21317875 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21317875}}
		+
		+	==About this Structure==
		+	[[3p23]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermomicrobium_roseum Thermomicrobium roseum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P23 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:21317875</ref><references group="xtra"/>
		+	[[Category: Thermomicrobium roseum]]
		+	[[Category: Ali, M M.U.]]
		+	[[Category: Pearl, L H.]]